Abstract:
:Ciliary ganglion neurons and nonneurons can be separated from each other, based on the greater adhesivity of the nonneurons to untreated tissue culture plastic in the presence of serum. When the separation was carried out in the presence of Serum Plus (a commercially available supplemented serum), two populations of neurons were distinguished. Neurons in the first class (50-60% of total) adhered to plastic within 15 min, tended to aggregate into clumps, and were not well supported in long term culture by brain extracts. Neuronal adhesion to plastic was inhibited by heparin but not by chondroitin sulfate. Neurons in the second class did not attach to plastic for up to 90 min (and could thus be purified), were not as prone to aggregation, and were quantitatively supported for long periods (greater than 2 weeks) by the neurotrophic factor(s) present in extracts of injured brain. Although no direct evidence is provided, these populations may correspond to the well characterized ciliary and choroid neurons.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Needels DL,Nieto-Sampedro M,Cotman CWdoi
10.1007/BF00966312subject
Has Abstractpub_date
1987-10-01 00:00:00pages
901-7issue
10eissn
0364-3190issn
1573-6903journal_volume
12pub_type
杂志文章abstract::The developmental profiles of acetylcholinesterase and choline acetyltransferase in chick optic tectum and retina cell aggregates, over a 30-day period, have been determined and compared with the corresponding developmental curves obtained in vivo. Both acetylcholinesterase and choline acetyltransferase activities in ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965466
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journal_title:Neurochemical research
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journal_title:Neurochemical research
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doi:10.1007/BF00965575
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journal_title:Neurochemical research
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更新日期:1991-01-01 00:00:00
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更新日期:2008-01-01 00:00:00
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更新日期:2010-02-01 00:00:00
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journal_title:Neurochemical research
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