Abstract:
:Using the immunoblot technique, we found that an incubation of purified human myelin in 10 mM Tris-HCl buffer at pH 7.5 resulted in the conversion of the myelin-associated glycoprotein (MAG) to a smaller derivative (dMAG). Exogenously added 5 mM CaCl2 accelerated the conversion of MAG. In buffer containing more than 100 microM of EGTA, the conversion was inhibited. In addition, the existence of endogenous calcium in purified myelin was confirmed using atomic absorption spectroscopy. The conversion was also inhibited partially by one of the thiol protease inhibitors, E-64 analogue (E-64-a). These observations suggest that the conversion of MAG is mediated by calcium-activated neutral protease (CANP)-like enzyme.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Sato S,Yanagisawa K,Miyatake Tdoi
10.1007/BF00964509subject
Has Abstractpub_date
1984-05-01 00:00:00pages
629-35issue
5eissn
0364-3190issn
1573-6903journal_volume
9pub_type
杂志文章abstract::The carnitine/acylcarnitine transporter is a transport system whose function is essential for the mitochondrial β-oxidation of fatty acids. Here, the presence of carnitine/acylcarnitine carrier (CACT) in nervous tissue and its sub-cellular localization in dorsal root ganglia (DRG) neurons have been investigated. Weste...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-1168-z
更新日期:2013-12-01 00:00:00
abstract::Ingestion of ammonium induces hyperammonemia which increases tubulin content in cerebrum but not in cerebellum. We have dissected 11 discrete areas of cerebrum and quantified the tubulin content in control and hyperammonemic rats. An heterogeneity in the induction of tubulin is shown. The areas more affected are ventr...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965516
更新日期:1989-12-01 00:00:00
abstract::We have developed a coupled Xenopus oocyte expression system for evaluating the functional effects of mutations in known or suspected adhesion molecules, which allows for a very rapid assessment of intercellular adhesion. As a model protein, we first used Protein zero (Po), an adhesion molecule that mediates self-adhe...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1010999622760
更新日期:2001-06-01 00:00:00
abstract::Suspensions of rat brain microsomes, synaptosomes, and synaptic vesicles were able to convert adenosine to inosine by means of adenosine deaminase. Isosbestic points of this transformation, at 222, 250 and 281 nm, remained unchanged with time-course. This fact suggests that adenosine deaminase (ADA, E.C. 3.5.4.4) is l...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965316
更新日期:1986-04-01 00:00:00
abstract::N-methyl-D-aspartate (NMDA) inhibits carbachol-stimulated phosphoinositide breakdown in rat brain cortical slices but not in isolated membranes (1). To gain insight into the mechanisms, we examined the effects of NMDA on carbachol-stimulated [3H]inositol phosphate and intermediates of phosphatidylinositol cycle accumu...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1022426204583
更新日期:1997-05-01 00:00:00
abstract::The influence of hypothyroidism (HO) induced by treatment with propylthiouracil on lipid composition, receptor responsiveness of M1-muscarinic receptors (M1AChRs) and metabotropic glutamate receptors (mGluRs) as well as on protein kinase C (PKC) activity was investigated in the cerebral cortex of Lewis rats. HO did no...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF02527750
更新日期:1996-05-01 00:00:00
abstract::Kainic acid-induced seizures produced early (2 hr) generalized edema and later (24 and 48 hr) necrotic edema in temporal cortex and hippocampus as measured by specific gravity changes. Mannitol given during the seizure partially protected against the early edema and prevented the necrotic edema indicating early edema ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF01000241
更新日期:1987-06-01 00:00:00
abstract::It is thought that changes in gene expression in the brain mediate chronic ethanol-induced complex behaviors such as tolerance, dependence, and sensitization, and also relate to ethanol-induced brain toxicity. Using high-density filter-based cDNA microarrays (GeneFilters), we analyzed the expression of over 5000 genes...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1020937728506
更新日期:2002-10-01 00:00:00
abstract::We analyzed two disease model groups with rats infected by Japanese encephalitis virus (JEV), a 90-day group and a 180-day group after JEV infection. The time measured by the modified pole test showed that motor activities in these two groups were slower than those of age-matched control groups. Striatal dopamine (DA)...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-006-9197-5
更新日期:2006-12-01 00:00:00
abstract::Endogenous phosphorylation of proteins from rat brain synaptosomal plasma membranes was studied in vitro. Cyclic AMP (cAMP) markedly stimulated(32)P incorporation in three protein bands with molecular weights of 75,000, 57,000, and 54,000, respectively. The effect of the behaviorally active peptide ACTH1-24 on this en...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965607
更新日期:1976-12-01 00:00:00
abstract::Protein kinase C has recently attracted considerable attention because of its importance in the control of cell division, cell differentiation, and signal transduction across the cell membrane. The activity of this enzyme is altered by several lipids such as diacylglycerol, free fatty acids, lipoxins, gangliosides, an...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/BF00973288
更新日期:1988-06-01 00:00:00
abstract::Changes in serotonin (5-HT) and 5-hydroxy indole acetic acid (5-HIAA), its major metabolite, in cerebral cortex, corpus striatum and hippocampus were investigated at 10th and 21st days of chronic ethanol ingestion in Wistar rats. Ethanol (7.2% v/v) was given to rats in a modified liquid diet. Biochemical analysis was ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1007579705230
更新日期:2000-02-01 00:00:00
abstract::Acrylamide, a prototype neurotoxin, alters neurofilament protein (NF) gene expression in rat brain. Levels of mRNA coding for neurofilament protein subunits NF-L, NF-M, and NF-H have been determined by Northern blot analysis using 32P-labeled cDNA probes. Acrylamide given acutely (100 mg/kg, single intraperitoneal inj...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00967449
更新日期:1994-07-01 00:00:00
abstract::Tumor Necrosis Factor Receptor Super Family 6 gene (TNFRSF6), also known as FAS, encodes the Fas antigen, a cell surface receptor mediating cell apoptosis, situated on chromosome 10q located near the region of linkage to sporadic Alzheimer's disease (sAD). FAS levels have been reported elevated in the brain of AD pati...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9329-6
更新日期:2007-09-01 00:00:00
abstract::Shape-shifting, a phenomenon wide-spread in folklore, refers to the ability to physically change from one identity to another, typically from an innocuous entity to a destructive one. The amino acid D-serine over the last 25 years has "shape-shifted" into several identities: a purported glial transmitter activating N-...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-020-03014-1
更新日期:2020-06-01 00:00:00
abstract::Olfactory ensheathing cells (OECs) and Schwann cells (SCs) are closely-related cell types with regeneration-promoting properties. Comparative gene expression analysis is particularly relevant since it may explain cell type-specific effects and guide the use of each cell type into special clinical applications. In the ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-1006-3
更新日期:2013-05-01 00:00:00
abstract::Adult rabbit brain contains the enzymatic machinery to convert deoxyuridine to deoxyuridine triphosphate (dUTP). Although dUTP as dUMP can be readily incorporated into DNA in place of thymidine monophosphate, we detected no (3H)dUMP in newly synthesized (3H)DNA in adult rabbit brain after the intraventricular injectio...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965201
更新日期:1983-08-01 00:00:00
abstract::Staurosporine was found to bring about complete growth inhibition of human glioma cell lines. U87 MG cells were arrested in S phase while U373 MG cells in G2/M phase on staurosporine treatment. Consistent with this observation, no change in G1 phase regulators viz., Cyclin D1, D3 and CDK4 was seen on staurosporine tre...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-006-9068-0
更新日期:2006-05-01 00:00:00
abstract::The experiments reported in this paper address the hypothesis that [3H]leucine enkephalin labels both mu and delta receptors. As reported by other workers, this peptide dissociates from rat brain membranes in a biphasic manner. This is consistent with a two site binding model which hypothesizes that the peptide labels...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00963918
更新日期:1983-02-01 00:00:00
abstract::Despite immense advances in the treatment strategies, the effective treatment of patients suffering from neuropathic pain remains challenging. Saikosaponin a possesses anti-inflammatory activity. However, the role of saikosaponin a in neuropathic pain is still unclear. Therefore, the objective of this study was to inv...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-014-1407-y
更新日期:2014-11-01 00:00:00
abstract::The lysosomal degradation of several sphingolipids requires the presence of four small glycoproteins called saposins, generated by proteolytic processing of a common precursor, prosaposin. Saposins share several structural properties, including six similarly located cysteines forming three disulfide bridges with the s...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1023/a:1022530508763
更新日期:1999-02-01 00:00:00
abstract::The current study investigated the neuroprotective activity of idebenone against pilocarpine-induced seizures and hippocampal injury in rats. Idebenone is a ubiquinone analog with antioxidant, and ATP replenishment effects. It is well tolerated and has low toxicity. Previous studies reported the protective effects of ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-014-1236-z
更新日期:2014-02-01 00:00:00
abstract::The effects of graded doses of D-pipecolic acid (0.005-5 micrograms/animals s.c.) on tolerance to the hypothermic effect of ethanol (4 g/kg i.p.) were investigated in mice. D-Pipecolic acid itself did not change the core temperature or the acute hypothermic response to a single dose of ethanol. Repeated D-pipecolic ac...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00967746
更新日期:1986-12-01 00:00:00
abstract::A previous study conducted in this laboratory revealed a decrease in total cholinesterase (total ChE) in the cerebral cortex, hippocampus and striatum in aged rats (24 months) of various strains, as compared with young animals (3 months). The purpose of the present experiments was to extend the study to other brain ar...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00969013
更新日期:1992-08-01 00:00:00
abstract::Tanshinone I (TsI) is an important lipophilic diterpene extracted from Danshen (Radix Salvia miltiorrhizae) and has been used in Asia for the treatment of cerebrovascular diseases such as ischemic stroke. In this study, we examined the neuroprotective effect of TsI against ischemic damage and its neuroprotective mecha...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-014-1312-4
更新日期:2014-07-01 00:00:00
abstract::Clinical studies have demonstrated that decreased adiponectin is associated with the development of Type 2 diabetes mellitus (T2DM) and Alzheimer's disease (AD). We focused on determining the neuroprotective effect offered by adiponectin against streptozotocin-induced brain damage in ICV-STZ rat model. We found that a...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-017-2426-2
更新日期:2018-02-01 00:00:00
abstract::The role of repulsive guidance molecule A (RGMa) in cerebral ischemia remains unclear. In the study, we examined the expression of RGMa in ischemic brain tissues following focal cerebral ischemia/reperfusion (IR) in rats. An established middle cerebral artery suture occlusion model was employed. A dipolar electrode wa...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-012-0809-y
更新日期:2012-09-01 00:00:00
abstract::Chlorpyrifos (CPF), an organophosphorus insecticide is known to cause ill health in non-target animals by inducing oxidative stress. In this study influence of cold stress (15°C and 20°C) and age as modulating factors on CPF induced oxidative stress was addressed to assess age-related differences and vulnerability in ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0311-3
更新日期:2011-02-01 00:00:00
abstract::Evidence was accumulated indicating that cyclic nucleotides are involved in regulation of growth, differentiation and function of lymphoid cells. It was previously shown that the N-fragment (1-4) of thymosin beta 4 (Ac-Ser-Asp-Lys-Pro-OH) inhibits in vivo the entry of cell populations into S-phase. In the course of th...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00995153
更新日期:1995-01-01 00:00:00
abstract::Epilepsy is a disorder of the brain characterized by an enduring predisposition to generate epileptic seizures. The glycolytic inhibitor 2-deoxy-D-glucose (2-DG) has been reported to exert antiepileptic effects by upregulating KATP subunits (kir6.1 and kir6.2). We evaluated whether 2-DG exhibits anti-seizure effect by...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-019-02734-3
更新日期:2019-04-01 00:00:00