Structural differences in the catalytic subunits of form I and form II ribulose 1,5-bisphosphate carboxylase/oxygenase from Rhodopseudomonas sphaeroides.

abstract：:The β-barrel assembly machinery (BAM) complex is the core machinery for the assembly of β-barrel membrane proteins, and inhibition of BAM complex activity is lethal to bacteria. Discovery of integral membrane proteins that are key to pathogenesis and yet do not require assistance from the BAM complex raises the questi...

journal_title：Journal of bacteriology

authors： Hay ID,Belousoff MJ,Dunstan RA,Bamert RS,Lithgow T

更新日期：2018-02-07 00:00:00

abstract：:We constructed a conditional mutant of pbpA in which transcription of the gene was placed under the control of an IPTG (isopropyl-beta-D-thiogalactopyranoside)-inducible promoter in order to explore the role of PBP1 in growth, cell wall structure, and cell division. A methicillin-resistant strain and an isogenic methi...

journal_title：Journal of bacteriology

authors： Pereira SF,Henriques AO,Pinho MG,de Lencastre H,Tomasz A

更新日期：2007-05-01 00:00:00

abstract：:The phoP-phoQ-regulated pagC locus is essential for full virulence and survival within macrophages of Salmonella typhimurium. The protein product, DNA sequence, and transcript of pagC were determined. The pagC locus encodes a single 188-amino-acid membrane protein that is similar to the ail-encoded eucaryotic cell inv...

journal_title：Journal of bacteriology

authors： Pulkkinen WS,Miller SI

更新日期：1991-01-01 00:00:00

abstract：:Escherichia coli W3110 contains four types of IS1 elements in the chromosome. Using an insertion element entrapping system, we collected 116 IS1 plasmid insertion mutants, which resulted from a minimum of 26 independent IS1 insertion events. All of them had insertions of IS1 of the IS1A (IS1E and IS1G) type. Inspectio...

journal_title：Journal of bacteriology

authors： Chen JH,Hsu WB,Hwang JL

更新日期：1998-10-01 00:00:00

abstract：:Micromonospora strains have been isolated from diverse niches, including soil, water, and marine sediments and root nodules of diverse symbiotic plants. In this work, we report the genome sequence of Micromonospora lupini Lupac 08 isolated from root nodules of the wild legume Lupinus angustifolious. ...

journal_title：Journal of bacteriology

authors： Alonso-Vega P,Normand P,Bacigalupe R,Pujic P,Lajus A,Vallenet D,Carro L,Coll P,Trujillo ME

更新日期：2012-08-01 00:00:00

abstract：:The fec region of the Escherichia coli chromosome determines a citrate-dependent iron(III) transport system. The nucleotide sequence of fec revealed five genes, fecABCDE, which are transcribed from fecA to fecE. The fecA gene encodes a previously described outer membrane receptor protein. The fecB gene product is form...

journal_title：Journal of bacteriology

authors： Staudenmaier H,Van Hove B,Yaraghi Z,Braun V

更新日期：1989-05-01 00:00:00

abstract：:Streptomyces coelicolor Müller contains two superoxide dismutases (SODs), nickel-containing (NiSOD) and iron- and zinc-containing SOD (FeZnSOD). The sodF gene encoding FeZnSOD was isolated by using PCR primers corresponding to the N-terminal peptide sequence of the purified FeZnSOD and a C-terminal region conserved am...

journal_title：Journal of bacteriology

authors： Kim EJ,Chung HJ,Suh B,Hah YC,Roe JH

更新日期：1998-04-01 00:00:00

abstract：:We have extended our previous studies of the DNA sequences required for growth rate-dependent control of rRNA transcription in Escherichia coli. Utilizing a reporter system suitable for evaluation of promoters with low activities, we have found that the core promoter region of rrnB P1 (-41 to +1 with respect to the tr...

journal_title：Journal of bacteriology

authors： Bartlett MS,Gourse RL

更新日期：1994-09-01 00:00:00

abstract：:We have identified three transposable gene-activating elements from Pseudomonas cepacia on the basis of their abilities to increase expression of the lac genes of the broad-host-range plasmid pGC91.14 (pRP1::Tn951). When introduced into auxotrophic derivatives of P. cepacia 249 (ATCC 17616), this plasmid failed to con...

journal_title：Journal of bacteriology

authors： Wood MS,Lory C,Lessie TG

更新日期：1990-04-01 00:00:00

abstract：:Firshein, W. (Wesleyan University, Middletown, Conn.). Effects of deoxyribonucleic acid products on deoxyribonucleic acid synthesis of virulent and avirulent pneumococci. J. Bacteriol. 82:169-180. 1961.-Cell suspensions of virulent pneumococci synthesize a greater amount of deoxyribonucleic acid (DNA) per cell in the ...

journal_title：Journal of bacteriology

authors： FIRSHEIN W

更新日期：1961-08-01 00:00:00

abstract：:The protein motive force of metabolizing Bacillus subtilis cells was only slightly affected by changes in the external pH between 5 and 8, although the electrical component and the chemical component of the proton motive force contributed differently at different external pH. The electrical component of the proton mot...

journal_title：Journal of bacteriology

authors： Shioi JI,Matsuura S,Imae Y

更新日期：1980-12-01 00:00:00

abstract：:UDP-N-acetylmuramic acid:L-alanine ligase (MurC) catalyzes the addition of the first amino acid to the cytoplasmic precursor of the bacterial cell wall peptidoglycan. The crystal structures of Haemophilus influenzae MurC in complex with its substrate UDP-N-acetylmuramic acid (UNAM) and Mg(2+) and of a fully assembled ...

journal_title：Journal of bacteriology

authors： Mol CD,Brooun A,Dougan DR,Hilgers MT,Tari LW,Wijnands RA,Knuth MW,McRee DE,Swanson RV

更新日期：2003-07-01 00:00:00

abstract：:The replication of the R factor Rts1 in Proteus mirabilis was examined by using the technique of CsCl density-gradient centrifugation. The proportion of Rts1 deoxyribonucleic acid (DNA) relative to the host chromosomal DNA (% R-DNA) was 7% in both exponential and stationary growth phases in Penassay Broth and suppleme...

journal_title：Journal of bacteriology

authors： Terawaki Y,Rownd R

更新日期：1972-02-01 00:00:00

abstract：:In response to molecular oxygen and/or fixed nitrogen, the product of the Klebsiella pneumoniae nitrogen fixation L (nifL) gene inhibits NifA-mediated transcriptional activation. Nitrogen regulation of NifL function occurs at two levels: transcription of the nifLA operon is regulated by the general Ntr system, and the...

journal_title：Journal of bacteriology

authors： He L,Soupene E,Kustu S

更新日期：1997-12-01 00:00:00

abstract：:Although competence normally develops only in glucose-minimal salts media, mecA and mecB mutations permit the expression of competence and of late competence genes in complex media as well (D. Dubnau and M. Roggiani, J. Bacteriol. 172:4048-4055, 1990). The expression of late competence genes is dependent on the produc...

journal_title：Journal of bacteriology

authors： Roggiani M,Hahn J,Dubnau D

更新日期：1990-07-01 00:00:00

abstract：:The major coat protein of the bacteriophage f1 is synthesized during infection of Escherichia coli and becomes tightly associated with the host membrane. This synthesis was studied in conjunction with the strain BB26-36, a mutant defective in phospholipid synthesis, to investigate basic questions concerning membrane p...

journal_title：Journal of bacteriology

authors： Cashman JS,Webster RE

更新日期：1977-03-01 00:00:00

abstract：:The heptapeptide-nucleotide microcin C (McC) targets aspartyl-tRNA synthetase. Upon its entry into a susceptible cell, McC is processed to release a nonhydrolyzable aspartyl-adenylate that inhibits aspartyl-tRNA synthetase, leading to the cessation of translation and cell growth. Here, we surveyed Escherichia coli cel...

journal_title：Journal of bacteriology

authors： Kazakov T,Vondenhoff GH,Datsenko KA,Novikova M,Metlitskaya A,Wanner BL,Severinov K

更新日期：2008-04-01 00:00:00

abstract：:Previous determination of the nucleotide sequence of the iol region of the Bacillus subtilis genome allowed us to predict the structure of the iol operon for myo-inositol catabolism, consisting of 10 iol genes (iolA to iouJ); iolG corresponds to idh, encoding myo-inositol 2-dehydrogenase (Idh). Primer extension analys...

journal_title：Journal of bacteriology

authors： Yoshida KI,Aoyama D,Ishio I,Shibayama T,Fujita Y

更新日期：1997-07-01 00:00:00

abstract：:One step in de novo pyridoxine (vitamin B6) and pyridoxal 5'-phosphate biosynthesis was predicted to be an oxidation catalyzed by an unidentified D-erythrose-4-phosphate dehydrogenase (E4PDH). To help identify this E4PDH, we purified the Escherichia coli K-12 gapA- and gapB-encoded dehydrogenases to homogeneity and te...

journal_title：Journal of bacteriology

authors： Zhao G,Pease AJ,Bharani N,Winkler ME

更新日期：1995-05-01 00:00:00

abstract：:Using an oligonucleotide microarray, we searched for previously unrecognized transcription units in intergenic regions in the genome of Bacillus subtilis, with an emphasis on identifying small genes activated during spore formation. Nineteen transcription units were identified, 11 of which were shown to depend on one ...

journal_title：Journal of bacteriology

authors： Schmalisch M,Maiques E,Nikolov L,Camp AH,Chevreux B,Muffler A,Rodriguez S,Perkins J,Losick R

更新日期：2010-10-01 00:00:00

abstract：:We have used Tn917lacZ to mutagenize the Bacillus subtilis chromosome and have isolated mutants that are defective in chemotaxis and motility. Mapping of the transposon inserts identified two new loci. Mutations in one of these loci generated mutants that had paralyzed flagella. Accordingly, we designate this a mot lo...

journal_title：Journal of bacteriology

authors： Zuberi AR,Ying CW,Parker HM,Ordal GW

更新日期：1990-12-01 00:00:00

abstract：:Synchronized cultures of Anacystis nidulans (Synechococcus PCC 6301), an obligate phototroph, are obtained by incubating exponential cultures in the dark for 12 to 16 h. A temporal and sequential order of macromolecular synthesis is observed within the cell division cycle of a synchronously dividing culture in the lig...

journal_title：Journal of bacteriology

authors： Asato Y

更新日期：1983-03-01 00:00:00

abstract：:The porin composition of the Escherichia coli cell envelope was analyzed during growth at different external pHs (pHo) as a function of the acetyl phosphate (AcP) level (DeltaackA pta or ackA mutant, pyruvate or glucose as the carbon source) in the presence or absence of EnvZ. Our results indicate that the AcP level i...

journal_title：Journal of bacteriology

authors： Heyde M,Laloi P,Portalier R

更新日期：2000-01-01 00:00:00

abstract：:Anabaena variabilis ATCC 29413 has one Mo nitrogenase that is made under oxic growth conditions in specialized cells called heterocysts and a second Mo nitrogenase that is made only under anoxic conditions in vegetative cells. The two large nif gene clusters responsible for these two nitrogenases are under the control...

journal_title：Journal of bacteriology

authors： Vernon SA,Pratte BS,Thiel T

更新日期：2017-01-30 00:00:00

abstract：:Lipid composition was studied in two strains each of mid-log phase cells of Bacillus thuringiensis, B. larvae, B. popilliae, B. alvei, and B. lentimorbus. Total lipids varied from 2.5 to 3.5% of the cell dry weight of B. thuringiensis to 4.3 to 5.0% of B. popilliae. Phospholipids in the organisms examined ranged from ...

journal_title：Journal of bacteriology

authors： Bulla LA,Bennett GA,Shotwell OL

更新日期：1970-12-01 00:00:00

abstract：:Using PCR to create a probe based on conserved region 2 of sigma factors, we have cloned the sigA gene coding for the major sigma factor of Rhizobium meliloti. The 684-residue protein encoded by the sigA gene was expressed in vitro in coupled transcription-translation experiments with R. meliloti extracts and migrated...

journal_title：Journal of bacteriology

authors： Rushing BG,Long SR

更新日期：1995-12-01 00:00:00

abstract：:Components of membranes isolated from Spiroplasma citri and corn stunt spiroplasma grown at 28 degrees C were analyzed. On a protein basis, lipid phosphorus was lower and cholesterol was higher in S. citri. Only minor differences between the two species were found in fatty acid composition, reduced nicotinamide adenin...

journal_title：Journal of bacteriology

authors： Mudd JB,Lee IM,Liu HY,Calavan EC

更新日期：1979-02-01 00:00:00

abstract：:Mutants lacking an enzyme of the oxidative branch of the hexose monophosphate shunt, 6-phosphogluconolactonase (pgl), have been selected as a new class of glucose-negative derivatives of a phosphoglucose isomerase (pgi) mutant. Glucose negativity is not as complete as in mutants lacking phosphoglucose isomerase and gl...

journal_title：Journal of bacteriology

authors： Kupor SR,Fraenkel DG

更新日期：1969-12-01 00:00:00

abstract：:Bacillus subtilis, which grows under aerobic conditions, employs fatty acid desaturase (Des) to fluidize its membrane when subjected to temperature downshift. Des requires molecular oxygen for its activity, and its expression is regulated by DesK-DesR, a two-component system. Transcription of des is induced by the tem...

journal_title：Journal of bacteriology

authors： Beranová J,Mansilla MC,de Mendoza D,Elhottová D,Konopásek I

更新日期：2010-08-01 00:00:00

abstract：:Several enzymes and metabolites concerned with carbohydrate metabolism were examined in mice infected with Listeria monocytogenes. Liver glycogen and glucose decreased parallel to severity of infection. The concentration of glucose in the blood fell to abnormally low levels with a hypoglycemia being most evident at 72...

journal_title：Journal of bacteriology

authors： Wilder MS,Sword CP

更新日期：1967-02-01 00:00:00