Physiology of Sporeforming Bacteria Associated with Insects II. Lipids of Vegetative Cells.

Abstract:

:Lipid composition was studied in two strains each of mid-log phase cells of Bacillus thuringiensis, B. larvae, B. popilliae, B. alvei, and B. lentimorbus. Total lipids varied from 2.5 to 3.5% of the cell dry weight of B. thuringiensis to 4.3 to 5.0% of B. popilliae. Phospholipids in the organisms examined ranged from 55 to 79% of total lipids; neutral lipids averaged from 13 to 45%. Common phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and lysophosphatidylethanolamine. 1,2-Diglycerides, methyl esters, free fatty acids, and hydrocarbons were found in all the organisms studied. Branched-chain fatty acids constituted more than 50% of the total fatty acids in B. thuringiensis, B. larvae, B. popilliae, and B. alvei, whereas, in B. lentimorbus, normal-chain acids constituted more than 50%. Anteiso-C(15) (12-methyltetradeconoate) was the most abundant acid (30 to 50%) in B. alvei, B. larvae, B. popilliae, and B. lentimorbus. In contrast, B. thuringiensis contained more iso-C(13) (7%), iso-C(15) (17%), normal-C(16) (24%), and iso-C(17) (18%) than anteiso-C(15) (6%). The distribution of individual fatty acids was similar in the phospholipids and neutral lipids of each organism. However, the total amount of iso, anteiso, and normal isomers differed.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Bulla LA,Bennett GA,Shotwell OL

doi

10.1128/JB.104.3.1246-1253.1970

keywords:

subject

Has Abstract

pub_date

1970-12-01 00:00:00

pages

1246-53

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

104

pub_type

杂志文章
  • Generic identification of L-forms by polyacrylamide gel electrophoretic comparison of extracts from parent strains and their derived L-forms.

    abstract::A polyacrylamide gel electrophoretic method was used for identification of L-forms in the genera Streptococcus, Staphylococcus, and Proteus, by comparing phenol-acetic acid-water extracts of homologous parent L-form pairs to one another and to other pairs. The method requires only milligram quantities of material for ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.1.71-77.1969

    authors: King JR,Theodore TS,Cole RM

    更新日期:1969-10-01 00:00:00

  • Regulation of acetate kinase isozymes and its importance for mixed-acid fermentation in Lactococcus lactis.

    abstract::Acetate kinase (ACK) converts acetyl phosphate to acetate along with the generation of ATP in the pathway for mixed-acid fermentation in Lactococcus lactis. The reverse reaction yields acetyl phosphate for assimilation purposes. Remarkably, L. lactis has two ACK isozymes, and the corresponding genes are present in an ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01277-13

    authors: Puri P,Goel A,Bochynska A,Poolman B

    更新日期:2014-04-01 00:00:00

  • Plasmid and transposon transfer to Thiobacillus ferrooxidans.

    abstract::The broad-host-range IncP plasmids RP4, R68.45, RP1::Tn501, and pUB307 were transferred to acidophilic, obligately chemolithotrophic Thiobacillus ferrooxidans from Escherichia coli by conjugation. A genetic marker of kanamycin resistance was expressed in T. ferrooxidans. Plasmid RP4 was transferred back to E. coli fro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.10.2892-2897.1994

    authors: Peng JB,Yan WM,Bao XZ

    更新日期:1994-05-01 00:00:00

  • Proteolytic adaptor for transfer-messenger RNA-tagged proteins from alpha-proteobacteria.

    abstract::We have identified an analog of SspB, the proteolytic adaptor for transfer-messenger RNA (tmRNA)-tagged proteins, in Caulobacter crescentus. C. crescentus SspB shares limited sequence similarity with Escherichia coli SspB but binds the tmRNA tag in vitro and is required for optimal proteolysis of tagged proteins in vi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01387-06

    authors: Lessner FH,Venters BJ,Keiler KC

    更新日期:2007-01-01 00:00:00

  • Competitive advantage provided by bacterial motility in the formation of nodules by Rhizobium meliloti.

    abstract::The effect of motility on the competitive success of Rhizobium meliloti in nodule production was investigated. A motile strain formed more nodules than expected when mixed at various unfavorable ratios with either flagellated or nonflagellated nonmotile derivatives. We conclude that motility confers a selective advant...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.148.2.728-729.1981

    authors: Ames P,Bergman K

    更新日期:1981-11-01 00:00:00

  • Function of S-adenosylmethionine in germinating yeast ascospores.

    abstract::Germination and outgrowth of ascospores of Saccharomyces cerevisiae 4579 require both methionine and adenine, whereas leucine is only required for outgrowth. The methionine requirement may be satisfied by S-adenosylmethionine, but this sulfonium compound will not substitute for adenine. Between 30 and 70 min of protei...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.131.1.63-68.1977

    authors: Choih SJ,Ferro AJ,Shapiro SK

    更新日期:1977-07-01 00:00:00

  • Recognition of DNA by three ferric uptake regulator (Fur) homologs in Bacillus subtilis.

    abstract::Bacillus subtilis contains three Fur homologs: Fur, PerR, and Zur. Despite significant sequence similarities, they respond to different stimuli and regulate different sets of genes. DNA target site comparisons indicate that all three paralogs recognize operators with a core 7-1-7 inverted repeat. The corresponding con...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.21.6348-6357.2003

    authors: Fuangthong M,Helmann JD

    更新日期:2003-11-01 00:00:00

  • Poly-beta-hydroxybutyrate membrane structure and its relationship to genetic transformability in Escherichia coli.

    abstract::The effects of competence-inducing treatments on the composition and organization of membrane lipids in Escherichia coli K-12, DH1, DH5, HB101, and RR1 were investigated for two widely used protocols in which transformability is developed at low temperatures in Ca2+ buffers. At stages during each procedure, the lipid ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.168.2.553-562.1986

    authors: Reusch RN,Hiske TW,Sadoff HL

    更新日期:1986-11-01 00:00:00

  • Identification of a secreted cholesterol-dependent cytolysin (mitilysin) from Streptococcus mitis.

    abstract::We have detected a cholesterol-dependent cytolysin, which we have named mitilysin, in a small number of Streptococcus mitis isolates. We have sequenced the mitilysin gene from seven isolates of S. mitis. Comparisons with the pneumococcal pneumolysin gene show 15 amino acid substitutions. S. mitis appear to release mit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01092-06

    authors: Jefferies J,Nieminen L,Kirkham LA,Johnston C,Smith A,Mitchell TJ

    更新日期:2007-01-01 00:00:00

  • Comparative structural and molecular characterization of ribitol-5-phosphate-containing Streptococcus oralis coaggregation receptor polysaccharides.

    abstract::The antigenically related coaggregation receptor polysaccharides (RPS) of Streptococcus oralis strains C104 and SK144 mediate recognition of these bacteria by other members of the dental plaque biofilm community. In the present study, the structure of strain SK144 RPS was established by high resolution NMR spectroscop...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01532-08

    authors: Yang J,Ritchey M,Yoshida Y,Bush CA,Cisar JO

    更新日期:2009-03-01 00:00:00

  • Identification and disruption of lisRK, a genetic locus encoding a two-component signal transduction system involved in stress tolerance and virulence in Listeria monocytogenes.

    abstract::lisRK encodes a two-component regulatory system in the food pathogen Listeria monocytogenes LO28. Following identification of the operon in an acid-tolerant Tn917 mutant, a deletion in the histidine kinase component was shown to result in a growth phase variation in acid tolerance, an ability to grow in high ethanol c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.21.6840-6843.1999

    authors: Cotter PD,Emerson N,Gahan CG,Hill C

    更新日期:1999-11-01 00:00:00

  • Initial proteome analysis of model microorganism Haemophilus influenzae strain Rd KW20.

    abstract::The proteome of Haemophilus influenzae strain Rd KW20 was analyzed by liquid chromatography (LC) coupled with ion trap tandem mass spectrometry (MS/MS). This approach does not require a gel electrophoresis step and provides a rapidly developed snapshot of the proteome. In order to gain insight into the central metabol...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.15.4593-4602.2003

    authors: Kolker E,Purvine S,Galperin MY,Stolyar S,Goodlett DR,Nesvizhskii AI,Keller A,Xie T,Eng JK,Yi E,Hood L,Picone AF,Cherny T,Tjaden BC,Siegel AF,Reilly TJ,Makarova KS,Palsson BO,Smith AL

    更新日期:2003-08-01 00:00:00

  • Infrequent cleavage of cloned Anabaena variabilis DNA by restriction endonucleases from A. variabilis.

    abstract::A cosmid vector has been constructed, using a lambda replicon. A library of cosmids from Anabaena variabilis ATCC 29413 based on use of this vector is shown to be highly deficient in sites for the two type II restriction endonucleases found in that organism. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.160.2.781-784.1984

    authors: Herrero A,Elhai J,Hohn B,Wolk CP

    更新日期:1984-11-01 00:00:00

  • Genomic insights into the convergence and pathogenicity factors of Campylobacter jejuni and Campylobacter coli species.

    abstract::Whether or not bacteria form coherent evolutionary groups via means of genetic exchange and, hence, elicit distinct species boundaries remains an unsettled issue. A recent report implied that not only may the former be true but also, in fact, the clearly distinct Campylobacter jejuni and Campylobacter coli species may...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00519-09

    authors: Caro-Quintero A,Rodriguez-Castaño GP,Konstantinidis KT

    更新日期:2009-09-01 00:00:00

  • Stimulation of deletions in the Escherichia coli chromosome by partially induced Mucts62 prophages.

    abstract::Deletion of bacterial DNA fragments is stimulated in induced Mucts62 lysogens. The host genes located proximally to the prophage are more frequently lost than those which are unlinked to the Mu genome. Genes located on either side of a Mu genome are deleted in the same manner. Like the other Mu-induced rearrangements,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.136.2.477-483.1978

    authors: Faelen M,Toussaint A

    更新日期:1978-11-01 00:00:00

  • Gene transfer to the desiccation-tolerant cyanobacterium Chroococcidiopsis.

    abstract::The coccoid cyanobacterium Chroococcidiopsis dominates microbial communities in the most extreme arid hot and cold deserts. These communities withstand constraints that result from multiple cycles of drying and wetting and/or prolonged desiccation, through mechanisms which remain poorly understood. Here we describe th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.7.2298-2305.2001

    authors: Billi D,Friedmann EI,Helm RF,Potts M

    更新日期:2001-04-01 00:00:00

  • Relation of catalase to substrate utilization by Mycoplasma pneumoniae.

    abstract::No catalase activity was detected in four strains of glucose-grown Mycoplasma pneumoniae at any time during the replication of the organism. Exogenous catalase dramatically increased the O(2) uptake with glycerol, presumably by releasing inhibition caused by hydrogen peroxide. The effect of added catalase on the O(2) ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.4.1425-1430.1968

    authors: Low IE,Eaton MD,Proctor P

    更新日期:1968-04-01 00:00:00

  • Gastrointestinal microflora of mutton birds (Puffinus tenuirostris) in relation to "limy" disease.

    abstract::Mushin, Rose (University of Melbourne, Victoria, Australia) and Frances M. Ashburner. Gastrointestinal microflora of mutton birds (Puffinus tenuirostris) in relation to "limy" disease. J. Bacteriol. 83:1260-1267. 1962.-The aerobic bacterial intestinal flora of mutton birds, healthy and affected with "limy" disease, wa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.6.1260-1267.1962

    authors: MUSHIN R,ASHBURNER FM

    更新日期:1962-06-01 00:00:00

  • Purification and properties of formylglutamate amidohydrolase from Pseudomonas putida.

    abstract::Formylglutamate amidohydrolase (FGase) catalyzes the terminal reaction in the five-step pathway for histidine utilization in Pseudomonas putida. By this action, N-formyl-L-glutamate (FG) is hydrolyzed to produce L-glutamate plus formate. Urocanate, the first product in the pathway, induced all five enzymes, but FG was...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.10.4696-4702.1987

    authors: Hu L,Mulfinger LM,Phillips AT

    更新日期:1987-10-01 00:00:00

  • Structural studies on lipoteichoic acids from four Listeria strains.

    abstract::The lipoteichoic acids were isolated from phenol extracts of four Listeria strains representing serotypes 4a, 4b, 6a, and 6 to compare the differences in structure of amphiphilic polysaccharides from various serotypes of Listeria spp. The lipoteichoic acids from the four strains examined had the same structure in both...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.168.1.115-122.1986

    authors: Uchikawa K,Sekikawa I,Azuma I

    更新日期:1986-10-01 00:00:00

  • Escherichia coli mutant Y16 is a double mutant carrying thermosensitive ftsH and ftsI mutations.

    abstract::The Escherichia coli mutant Y16, which shows thermosensitive colony formation and filamentation with reduced amounts of penicillin-binding protein 3 (PBP3), has mutations in the ftsI gene encoding PBP3 and in the ftsH gene. The ftsI mutation markedly reduces the amount of PBP3 at 42 degrees C, whereas the amount of th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.7.2416-2417.1992

    authors: Begg KJ,Tomoyasu T,Donachie WD,Khattar M,Niki H,Yamanaka K,Hiraga S,Ogura T

    更新日期:1992-04-01 00:00:00

  • Disruption of a rhodaneselike gene results in cysteine auxotrophy in Saccharopolyspora erythraea.

    abstract::A 3,373-base-pair DNA segment from a clone fortuitously isolated from Saccharopolyspora erythraea by hybridization to an oligodeoxynucleotide probe was sequenced. Computer-assisted analysis of the nucleotide sequence reveals three closely linked Streptomyces open reading frames plus a fourth converging on the others. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.1.350-360.1990

    authors: Donadio S,Shafiee A,Hutchinson CR

    更新日期:1990-01-01 00:00:00

  • Regulated nitrate transport in the cyanobacterium Anacystis nidulans.

    abstract::Intracellular accumulation of nitrate, indicative of the operation of an active nitrate transport system, has been measured in intact cells of the cyanobacterium Anacystis nidulans. The ability of the cells to accumulate nitrate was effectively hindered by either ammonium addition or selective inhibition of CO2 fixati...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.9.4376-4378.1987

    authors: Lara C,Romero JM,Guerrero MG

    更新日期:1987-09-01 00:00:00

  • Mechanism of tuberculostasis in mammalian serum. II. Induction of serum tuberculostasis in guinea pigs.

    abstract::The growth of tubercle bacilli in serum samples of untreated animals depends upon the availability of ionic iron which serves as a growth factor in supporting bacillary multiplication. The amount of available iron in serum is determined by the ratio between iron-saturated and iron-free transferrin; a low value for the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.1.64-70.1969

    authors: Kochan I,Golden CA,Bukovic JA

    更新日期:1969-10-01 00:00:00

  • Regulation of expression of the Escherichia coli K-12 mtr gene by TyrR protein and Trp repressor.

    abstract::The Escherichia coli K-12 mtr gene, which encodes a tryptophan-specific permease, was cloned, and its nucleotide sequence was determined. The precise location of the mtr gene at 69 min on the E. coli chromosome was determined. The mtr gene product was identified as a 414-amino-acid residue protein with a calculated mo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.13.4133-4143.1991

    authors: Sarsero JP,Wookey PJ,Pittard AJ

    更新日期:1991-07-01 00:00:00

  • Characterization of a gene, glnL, the product of which is involved in the regulation of nitrogen utilization in Escherichia coli.

    abstract::DNA was prepared from a strain of Escherichia coli bearing a mutation which confers the GlnC phenotype (inability to reduce the expression of glnA and other nitrogen-regulated operons in response to ammonia in the growth medium). A fragment of this DNA carrying glnA, the structural gene for glutamine synthetase, was c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.150.1.214-220.1982

    authors: Chen YM,Backman K,Magasanik B

    更新日期:1982-04-01 00:00:00

  • A Ti plasmid-encoded enzyme required for degradation of mannopine is functionally homologous to the T-region-encoded enzyme required for synthesis of this opine in crown gall tumors.

    abstract::The mocC gene encoded by the octopine/mannityl opine-type Ti plasmid pTi15955 is related at the nucleotide sequence level to mas1' encoded by the T region of this plasmid. While Mas1 is required for the synthesis of mannopine (MOP) by crown gall tumor cells, MocC is essential for the utilization of MOP by Agrobacteriu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.11.3285-3292.1996

    authors: Kim KS,Chilton WS,Farrand SK

    更新日期:1996-06-01 00:00:00

  • Influences of capsule on cell shape and chain formation of wild-type and pcsB mutants of serotype 2 Streptococcus pneumoniae.

    abstract::PcsB is a protein of unknown function that plays a critical role in cell division in Streptococcus pneumoniae and other ovococcus species of Streptococcus. We constructed isogenic sets of mutants expressing different amounts of PcsB in laboratory strain R6 and virulent serotype 2 strain D39 to evaluate its cellular ro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01505-08

    authors: Barendt SM,Land AD,Sham LT,Ng WL,Tsui HC,Arnold RJ,Winkler ME

    更新日期:2009-05-01 00:00:00

  • Sequence of molecular events involved in induction of allophanate hydrolase.

    abstract::Addition of urea to an uninduced culture of Saccharomyces at 22 C results in appearance of allophanate hydrolase activity after a lag of 12 min. We have previously demonstrated that both ribonucleic acid (RNA) and protein synthesis are needed for this induction to occur. To elucidate the time intervals occupied by kno...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.126.1.198-204.1976

    authors: Bossinger J,Cooper TG

    更新日期:1976-04-01 00:00:00

  • The legacy of HfrH: mutations in the two-component system CreBC are responsible for the unusual phenotype of an Escherichia coli arcA mutant.

    abstract::Strains derived from HfrH carrying the arcA2 null mutation exhibit a higher respiratory rate, enhanced glucose consumption, and a more-reduced intracellular redox state than arcA deletion mutants of a different lineage. The phenotype of the arcA2 mutants was due to the presence of a creC constitutive mutation introduc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00040-08

    authors: Nikel PI,de Almeida A,Pettinari MJ,Méndez BS

    更新日期:2008-05-01 00:00:00