Regulation of acetate kinase isozymes and its importance for mixed-acid fermentation in Lactococcus lactis.

Abstract:

:Acetate kinase (ACK) converts acetyl phosphate to acetate along with the generation of ATP in the pathway for mixed-acid fermentation in Lactococcus lactis. The reverse reaction yields acetyl phosphate for assimilation purposes. Remarkably, L. lactis has two ACK isozymes, and the corresponding genes are present in an operon. We purified both enzymes (AckA1 and AckA2) from L. lactis MG1363 and determined their oligomeric state, specific activities, and allosteric regulation. Both proteins form homodimeric complexes, as shown by size exclusion chromatography and static light-scattering measurements. The turnover number of AckA1 is about an order of magnitude higher than that of AckA2 for the reaction in either direction. The Km values for acetyl phosphate, ATP, and ADP are similar for both enzymes. However, AckA2 has a higher affinity for acetate than does AckA1, suggesting an important role under acetate-limiting conditions despite the lower activity. Fructose-1,6-bisphosphate, glyceraldehyde-3-phosphate, and phospho-enol-pyruvate inhibit the activities of AckA1 and AckA2 to different extents. The allosteric regulation of AckA1 and AckA2 and the pool sizes of the glycolytic intermediates are consistent with a switch from homolactic to mixed-acid fermentation upon slowing of the growth rate.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Puri P,Goel A,Bochynska A,Poolman B

doi

10.1128/JB.01277-13

subject

Has Abstract

pub_date

2014-04-01 00:00:00

pages

1386-93

issue

7

eissn

0021-9193

issn

1098-5530

pii

JB.01277-13

journal_volume

196

pub_type

杂志文章
  • Energetics of glycylglycine transport in Escherichia coli.

    abstract::The transport system for glycylglycine in Escherichia coli behaves like a shock-sensitive transport system. The initial rate of transport is reduced 85% by subjecting whole cells to osmotic shock, and glycylglycine is not transported by membrane vesicles. The energetics of transport was studied with strain ML 308-225 ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.120.1.139-146.1974

    authors: Cowell JL

    更新日期:1974-10-01 00:00:00

  • Genomic interrogation of the dassie bacillus reveals it as a unique RD1 mutant within the Mycobacterium tuberculosis complex.

    abstract::Despite their remarkable genetic homology, members of the Mycobacterium tuberculosis complex express very different phenotypes, most notably in their spectra of clinical presentation. For example, M. tuberculosis is regarded as pathogenic to humans, whereas members having deleted RD1, such as Mycobacterium microti and...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.1.104-109.2003

    authors: Mostowy S,Cousins D,Behr MA

    更新日期:2004-01-01 00:00:00

  • A collagen-binding adhesin, Acb, and ten other putative MSCRAMM and pilus family proteins of Streptococcus gallolyticus subsp. gallolyticus (Streptococcus bovis Group, biotype I).

    abstract::Members of the Streptococcus bovis group are important causes of endocarditis. However, factors associated with their pathogenicity, such as adhesins, remain uncharacterized. We recently demonstrated that endocarditis-derived Streptococcus gallolyticus subsp. gallolyticus isolates frequently adhere to extracellular ma...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00909-09

    authors: Sillanpää J,Nallapareddy SR,Qin X,Singh KV,Muzny DM,Kovar CL,Nazareth LV,Gibbs RA,Ferraro MJ,Steckelberg JM,Weinstock GM,Murray BE

    更新日期:2009-11-01 00:00:00

  • Complete genome sequence of Leuconostoc mesenteroides subsp. mesenteroides strain J18, isolated from kimchi.

    abstract::Leuconostoc mesenteroides subsp. mesenteroides is one of the most predominant lactic acid bacterial groups during kimchi fermentation. Here, we report the complete genome sequence of L. mesenteroides subsp. mesenteroides J18, which was isolated from kimchi. The genome of the strain consists of a 1,896,561-bp chromosom...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06498-11

    authors: Jung JY,Lee SH,Lee SH,Jeon CO

    更新日期:2012-02-01 00:00:00

  • Repair of extensive ionizing-radiation DNA damage at 95 degrees C in the hyperthermophilic archaeon Pyrococcus furiosus.

    abstract::We investigated the capacity of the hyperthermophile Pyrococcus furiosus for DNA repair by measuring survival at high levels of 60Co gamma-irradiation. The P. furiosus 2-Mb chromosome was fragmented into pieces ranging from 500 kb to shorter than 30 kb at a dose of 2,500 Gy and was fully restored upon incubation at 95...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.14.4643-4645.1997

    authors: DiRuggiero J,Santangelo N,Nackerdien Z,Ravel J,Robb FT

    更新日期:1997-07-01 00:00:00

  • Factors affecting growth and nitrogen fixation of Spirillum lipoferum.

    abstract::Spirillum lipoferum grows vigorously on malate, succinate, lactate, or pyruvate, moderately on galactose or acetate, and poorly on glucose or citrate. It reduces 15N2. Acetylene reduction rates decrease rapidly when the pH of the culture rises above 7.8. The organism is highly aerobic and had doubling times as low as ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.3.1248-1254.1976

    authors: Okon Y,Albrecht SL,Burris RH

    更新日期:1976-09-01 00:00:00

  • Correlation between acrasins and spore germination inhibitors in cellular slime molds.

    abstract::Discadenine,3-(3-amino-3-carboxypropyl)-N6-delta 2-isopentenyladenine, which inhibits spore germination, was previously found in Dictyostelium discoideum. Studies on the distribution of discadenine in different species of cellular slime molds by high-pressure liquid chromatography showed that discadenine is present in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.143.2.715-719.1980

    authors: Taya Y,Yamada T,Nishimura S

    更新日期:1980-08-01 00:00:00

  • Deletion of the 389 N-terminal residues of the transcriptional activator AREA does not result in nitrogen metabolite derepression in Aspergillus nidulans.

    abstract::Utilizing a homologous gene replacement in order to retain the native promoter and 5' and 3' untranslated messenger regions (and thereby ensure physiological validity), we have shown that deletion of the N-terminal 389 amino acids of the transcriptional activator AREA does not result in nitrogen metabolite derepressio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.21.5762-5764.1998

    authors: Caddick MX,Arst HN Jr

    更新日期:1998-11-01 00:00:00

  • INDUCIBLE RESISTANCE TO ERYTHROMYCIN IN STAPHYLOCOCCUS AUREUS.

    abstract::Weaver, Judith R. (Iowa State University, Ames), and P. A. Pattee. Inducible resistance to erythromycin in Staphylococcus aureus. J. Bacteriol. 88:574-580. 1964.-The dissociated resistance of Staphylococcus aureus to erythromycin was examined and was found to possess the characteristics of an inducible enzyme. The ind...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.3.574-580.1964

    authors: WEAVER JR,PATTEE PA

    更新日期:1964-09-01 00:00:00

  • Organization and transcriptional regulation of the Escherichia coli K-12 D-serine tolerance locus.

    abstract::We have reinvestigated the genetic organization and the transcription regulation of the dsd operon of Escherichia coli. By combining genetic and biochemical studies, it is demonstrated that the regulatory region of the operon and the gene encoding the specific regulator of D-serine tolerance (dsdC) had been misplaced ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.22.6456-6461.1995

    authors: Nørregaard-Madsen M,McFall E,Valentin-Hansen P

    更新日期:1995-11-01 00:00:00

  • c-di-AMP Accumulation Impairs Muropeptide Synthesis in Listeria monocytogenes.

    abstract::Cyclic di-AMP (c-di-AMP) is an essential and ubiquitous second messenger among bacteria. c-di-AMP regulates many cellular pathways through direct binding to several molecular targets in bacterial cells. c-di-AMP depletion is well known to destabilize the bacterial cell wall, resulting in increased bacteriolysis and en...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00307-20

    authors: Massa SM,Sharma AD,Siletti C,Tu Z,Godfrey JJ,Gutheil WG,Huynh TN

    更新日期:2020-11-19 00:00:00

  • Multiple formate dehydrogenase enzymes in the facultative methylotroph Methylobacterium extorquens AM1 are dispensable for growth on methanol.

    abstract::Formate dehydrogenase has traditionally been assumed to play an essential role in energy generation during growth on C(1) compounds. However, this assumption has not yet been experimentally tested in methylotrophic bacteria. In this study, a whole-genome analysis approach was used to identify three different formate d...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.1.22-28.2004

    authors: Chistoserdova L,Laukel M,Portais JC,Vorholt JA,Lidstrom ME

    更新日期:2004-01-01 00:00:00

  • Interaction of the Gifsy-1 Xis protein with the Gifsy-1 attP sequence.

    abstract::The Gifsy-1 phage integrates site specifically into the Salmonella chromosome via an integrase-mediated site-specific recombination mechanism. Initial genetic analysis suggests that Gifsy-1 integrase-mediated excision of the Gifsy-1 phage is influenced by proteins encoded by both the Gifsy-1 and the Gifsy-2 phages. Ou...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00577-07

    authors: Flanigan A,Gardner JF

    更新日期:2007-09-01 00:00:00

  • PilMNOPQ from the Pseudomonas aeruginosa type IV pilus system form a transenvelope protein interaction network that interacts with PilA.

    abstract::Pseudomonas aeruginosa type IV pili (T4P) are virulence factors that promote infection of cystic fibrosis and immunosuppressed patients. As the absence of T4P impairs colonization, they are attractive targets for the development of novel therapeutics. Genes in the pilMNOPQ operon are important for both T4P assembly an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00032-13

    authors: Tammam S,Sampaleanu LM,Koo J,Manoharan K,Daubaras M,Burrows LL,Howell PL

    更新日期:2013-05-01 00:00:00

  • The ABC-type multidrug resistance transporter LmrCD is responsible for an extrusion-based mechanism of bile acid resistance in Lactococcus lactis.

    abstract::Upon prolonged exposure to cholate and other toxic compounds, Lactococcus lactis develops a multidrug resistance phenotype that has been attributed to an elevated expression of the heterodimeric ABC-type multidrug transporter LmrCD. To investigate the molecular basis of bile acid resistance in L. lactis and to evaluat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00485-08

    authors: Zaidi AH,Bakkes PJ,Lubelski J,Agustiandari H,Kuipers OP,Driessen AJ

    更新日期:2008-11-01 00:00:00

  • Tripartite Regulation of the glpFKD Operon Involved in Glycerol Catabolism by GylR, Crp, and SigF in Mycobacterium smegmatis.

    abstract::The glpD (MSMEG_6761) gene encoding glycerol-3-phosphate dehydrogenase was shown to be crucial for M. smegmatis to utilize glycerol as the sole carbon source. The glpD gene likely forms the glpFKD operon together with glpF and glpK, encoding a glycerol facilitator and glycerol kinase, respectively. The gylR (MSMEG_675...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00511-19

    authors: Bong HJ,Ko EM,Song SY,Ko IJ,Oh JI

    更新日期:2019-11-20 00:00:00

  • Conserved cytoplasmic loops are important for both the transport and chemotaxis functions of PcaK, a protein from Pseudomonas putida with 12 membrane-spanning regions.

    abstract::Chemotaxis to the aromatic acid 4-hydroxybenzoate (4-HBA) by Pseudomonas putida is mediated by PcaK, a membrane-bound protein that also functions as a 4-HBA transporter. PcaK belongs to the major facilitator superfamily (MFS) of transport proteins, none of which have so far been implicated in chemotaxis. Work with two...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.16.5068-5074.1999

    authors: Ditty JL,Harwood CS

    更新日期:1999-08-01 00:00:00

  • EFFECT OF SUGARS AND OTHER CARBON COMPOUNDS ON GERMINATION AND POSTGERMINATIVE DEVELOPMENT OF BACILLUS MEGATERIUM SPORES.

    abstract::Hyatt, Mildred T. (Pioneering Research Division. U.S. Army Natick Laboratories, Natick, Mass.), and Hillel S. Levinson. Effect of sugars and other carbon compounds on germination and postgerminative development of Bacillus megaterium spores. J. Bacteriol. 88:1403-1415. 1964.-A total of 77 carbon-containing compounds w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.5.1403-1415.1964

    authors: HYATT MT,LEVINSON HS

    更新日期:1964-11-01 00:00:00

  • Biochemical and serological evidence for an RNase E-like activity in halophilic Archaea.

    abstract::Endoribonuclease RNase E appears to control the rate-limiting step that mediates the degradation of many mRNA species in bacteria. In this work, an RNase E-like activity in Archaea is described. An endoribonucleolytic activity from the extreme halophile Haloarcula marismortui showed the same RNA substrate specificity ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.4.1180-1185.1997

    authors: Franzetti B,Sohlberg B,Zaccai G,von Gabain A

    更新日期:1997-02-01 00:00:00

  • Induction and repression of nitrate reductase in Neurospora crassa.

    abstract::Synthesis of wild-type Neurospora crassa assimilatory nitrate reductase is induced in the presence of nitrate ions and repressed in the presence of ammonium ions. Effects of several Neurospora mutations on the regulation of this enzyme are shown: (i) the mutants, nit-1 and nit-3, involving separate lesions, lack reduc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.133.2.671-679.1978

    authors: Dantzig AH,Zurowski WK,Ball TM,Nason A

    更新日期:1978-02-01 00:00:00

  • CsgA, an extracellular protein essential for Myxococcus xanthus development.

    abstract::CsgA mutants of Myxococcus xanthus appear to be defective in producing an extracellular molecule essential for the developmental behaviors of this bacterium. The csgA gene encodes a 17.7-kilodalton polypeptide whose function and cellular location were investigated with immunological probes. Large quantities of the Csg...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.9.5299-5306.1990

    authors: Shimkets LJ,Rafiee H

    更新日期:1990-09-01 00:00:00

  • Cell wall of Mycobacterium lepraemurium strain Hawaii.

    abstract::The chemical properties of the cell wall of Mycobacterium lepraemurium strain Hawaii were investigated. Five subunits of the cell wall, arabinose mycolate, mycolic acids, tetrapeptide (Ala-Gln-diaminopimelic acid-Ala), disaccharide (N-acetylglucosaminyl-beta-1,4-N-glycolylmuramic acid), and arabinogalactan, were obtai...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.1.515-518.1973

    authors: Azuma I,Yamamura Y,Tanaka Y,Kosaka K,Mori T

    更新日期:1973-01-01 00:00:00

  • Uptake of N,N'-diacetylchitobiose [(GlcNAc)2] via the phosphotransferase system is essential for chitinase production by Serratia marcescens 2170.

    abstract::The chiR gene of Serratia marcescens 2170, encoding a LysR-type transcriptional activator, was identified previously as an essential factor for expression of chitinases and a chitin-binding protein, CBP21. To identify other genes that are essential for chitinase production, transposon mutagenesis with mini-Tn5Km1 was ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.6.1776-1782.2003

    authors: Uchiyama T,Kaneko R,Yamaguchi J,Inoue A,Yanagida T,Nikaidou N,Regue M,Watanabe T

    更新日期:2003-03-01 00:00:00

  • Amplification of lac cannot account for adaptive mutation to Lac+ in Escherichia coli.

    abstract::When the Lac- strain of Escherichia coli, FC40, is incubated with lactose as its sole carbon and energy source, Lac+ revertants arise at a constant rate, a phenomenon known as adaptive mutation. Two alternative models for adaptive mutation have been proposed: (i) recombination-dependent mutation, which specifies that ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01706-06

    authors: Stumpf JD,Poteete AR,Foster PL

    更新日期:2007-03-01 00:00:00

  • Natural transformation in Campylobacter species.

    abstract::Growing cells of Campylobacter coli and C. jejuni were naturally transformed by naked DNA without the requirement for any special treatment. Transformation frequencies for homologous chromosomal DNA were approximately 10(-3) transformants per recipient cell in C. coli and 10(-4) in C. jejuni. Maximum competence was fo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.2.949-955.1990

    authors: Wang Y,Taylor DE

    更新日期:1990-02-01 00:00:00

  • Periplasmic nitrate reductase (NapABC enzyme) supports anaerobic respiration by Escherichia coli K-12.

    abstract::Periplasmic nitrate reductase (NapABC enzyme) has been characterized from a variety of proteobacteria, especially Paracoccus pantotrophus. Whole-genome sequencing of Escherichia coli revealed the structural genes napFDAGHBC, which encode NapABC enzyme and associated electron transfer components. E. coli also expresses...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.5.1314-1323.2002

    authors: Stewart V,Lu Y,Darwin AJ

    更新日期:2002-03-01 00:00:00

  • Pyrrolidone carboxylyl peptidase in Streptococcus cremoris: dependence on an interaction with membrane components.

    abstract::A study of the distribution of pyrrolidone carboxylyl peptidase (PCP) activity among cell fractions of Streptococcus cremoris HP revealed that this enzyme is associated with a particulate fraction, which mainly consists of membrane material. This location could only be established using a gentle nonmechanical method f...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.3.1281-1288.1977

    authors: Exterkate FA

    更新日期:1977-03-01 00:00:00

  • Aspartate transcarbamylase synthesis ceases prior to inactivation of the enzyme in Bacillus subtilis.

    abstract::Aspartate transcarbamylase is synthesized during exponential growth of Bacillus subtilis and is inactivated when the cells enter the stationary phase. This work is a study of the regulation of aspartate transcarbamylase synthesis during growth and the stationary phase. Using specific immunoprecipitation of aspartate t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.135.3.943-951.1978

    authors: Maurizi MR,Switzer RL

    更新日期:1978-09-01 00:00:00

  • Anaerobiosis induces expression of ant, a new Escherichia coli locus with a role in anaerobic electron transport.

    abstract::Escherichia coli has a formate hydrogenlyase system which allows it to maintain an electron balance during anaerobic growth by passing electrons from formate to H+ ions, thus generating H2. The Mu d1(Ap lac) bacteriophage was used to generate mutants that were defective in passing electrons from formate to benzyl viol...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.158.1.180-186.1984

    authors: Yerkes JH,Casson LP,Honkanen AK,Walker GC

    更新日期:1984-04-01 00:00:00

  • Genome sequence of lineage III Listeria monocytogenes strain HCC23.

    abstract::More than 98% of reported human listeriosis cases are caused by Listeria monocytogenes serotypes within lineages I and II. Serotypes within lineage III (4a and 4c) are commonly isolated from environmental and food specimens. We report the first complete genome sequence of a lineage III isolate, HCC23, which will be us...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05236-11

    authors: Steele CL,Donaldson JR,Paul D,Banes MM,Arick T,Bridges SM,Lawrence ML

    更新日期:2011-07-01 00:00:00