Abstract:
:CsgA mutants of Myxococcus xanthus appear to be defective in producing an extracellular molecule essential for the developmental behaviors of this bacterium. The csgA gene encodes a 17.7-kilodalton polypeptide whose function and cellular location were investigated with immunological probes. Large quantities of the CsgA gene product were obtained from a lacZ-csgA translational gene fusion expressed in Escherichia coli. The chimeric 21-kilodalton protein was purified by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Affinity-purified polyclonal antibodies raised against the fusion protein were used to determine the cellular location of the native CsgA protein by colloidal gold labeling and transmission electron microscopy. Between 1,100 and 2,200 extracellular molecules of CsgA per developing M. xanthus cell were detected, most of which were associated with the extracellular matrix. The anti-CsgA antibodies inhibited wild-type development unless they were first neutralized with the fusion protein. Together these results suggest that the CsgA gene product has an essential, extracellular function during development, possibly as a pheromone.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Shimkets LJ,Rafiee Hdoi
10.1128/jb.172.9.5299-5306.1990subject
Has Abstractpub_date
1990-09-01 00:00:00pages
5299-306issue
9eissn
0021-9193issn
1098-5530journal_volume
172pub_type
杂志文章abstract::Studies of gene expression using fusions to lacZ demonstrated that the Bacillus subtilis dacB gene, encoding penicillin-binding protein 5*, is in an operon with two downstream genes, spmA and spmB. Mutations affecting any one of these three genes resulted in the production of spores with reduced heat resistance. The c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.16.4721-4729.1995
更新日期:1995-08-01 00:00:00
abstract::We isolated and characterized crp mutations in Escherichia coli that allow cyclic AMP (cAMP) receptor protein to function without cAMP. These mutants defined a region involved in the cAMP-induced allosteric change of cAMP receptor protein that is necessary for activation of the protein. Currently, we have isolated int...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.4.1417-1422.1988
更新日期:1988-04-01 00:00:00
abstract::The production of extracellular enzymes such as pectate lyase (Pel), polygalacturonase (Peh), cellulase (Cel), and protease (Prt) is activated by the cell density (quorum)-sensing signal, N-(3-oxohexanoyl)-L-homoserine lactone (HSL); plant signals; and aep genes during postexponential growth of Erwinia carotovora subs...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.17.5108-5115.1995
更新日期:1995-09-01 00:00:00
abstract::Production of Bacillus subtilis exoproteases is positively regulated by the DegS-DegU two-component regulatory system and other regulatory factors including DegR and ProB. It was shown that the expression of degR was virtually abolished in a sigD mutant and that the transcriptional initiation site in vivo is preceded ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.1.216-222.1996
更新日期:1996-01-01 00:00:00
abstract::A pLAFR3 cosmid clone designated pVir2 containing a 25-kilobase (kb) DNA insert was isolated from a wild-type Pseudomonas solanacearum GMI1000 genomic library. This cosmid was shown to complement all but one of the nine Tn5-induced mutants which have been isolated after random mutagenesis and which have lost both path...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.12.5626-5632.1987
更新日期:1987-12-01 00:00:00
abstract::The TatA and TatB proteins are essential components of the twin arginine protein translocation pathway in Escherichia coli. C-terminal truncation analysis of the TatA protein revealed that a plasmid-expressed TatA protein shortened by 40 amino acids is still fully competent to support protein translocation. Similar tr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.21.5871-5879.2002
更新日期:2002-11-01 00:00:00
abstract::The promoter region of the pColV-K30-encoded operon specifying biosynthesis and transport of the siderophore aerobactin was subjected to deletion analysis to determine the smallest DNA sequence affording iron regulation of a iucA'-'lacZ gene fusion. A 78-base-pair (bp) region containing the main (P1) promoter retained...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.6.2624-2630.1987
更新日期:1987-06-01 00:00:00
abstract::Choline starvation of the Neurospora crassa chol-1 mutant leads to a decrease in respiration through the cytochrome chain and a concomitant induction of the alternate oxidase. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.126.1.542-543.1976
更新日期:1976-04-01 00:00:00
abstract::Clostridium perfringens is an anaerobic Gram-positive pathogen that causes many human and animal diseases, including food poisoning and gas gangrene. C. perfringens lacks flagella but possesses type IV pili (TFP). We have previously shown that C. perfringens can glide across an agar surface in long filaments composed ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01288-12
更新日期:2013-02-01 00:00:00
abstract::The organization and transcriptional control of chromosomal cat genes (required for dissimilation of catechol by the beta-ketoadipate pathway) in the Pseudomonas putida biotype strain (ATCC 12633) are reported. Nucleotide sequence reveals that catR is separated by 135 bp from the divergently transcribed catBC,A; catC ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.2.401-412.1995
更新日期:1995-01-01 00:00:00
abstract::The antigenically related coaggregation receptor polysaccharides (RPS) of Streptococcus oralis strains C104 and SK144 mediate recognition of these bacteria by other members of the dental plaque biofilm community. In the present study, the structure of strain SK144 RPS was established by high resolution NMR spectroscop...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01532-08
更新日期:2009-03-01 00:00:00
abstract::The acetogenic bacterium Clostridium thermoautotrophicum, grown on methanol, glucose, or CO2-H2, contained high levels of corrinoids, formate dehydrogenase, tetrahydrofolate enzymes, carbon monoxide dehydrogenase, and hydrogenase. Cell-free extracts catalyzed pyruvate-dependent formation of acetate from methyltetrahyd...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.1.507-509.1982
更新日期:1982-07-01 00:00:00
abstract::We report the draft genome sequence of Lactobacillus salivarius SMXD51, isolated from the cecum of healthy chickens showing an activity against Campylobacter--the food-borne pathogen that is the most common cause of gastroenteritis in the European Union (EU)--and potentially interesting features for a probiotic strain...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00344-12
更新日期:2012-06-01 00:00:00
abstract::Three polynucleotide phosphorylase mutations, isolated in heavily mutagenized Escherichia coli strains Q7, Q13, and Q27, were characterized after their transfer by P1 transduction to nearly isogenic strains which lack ribonuclease I. Each strain has a different altered form of polynucleotide phosphorylase. One enzyme ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.3.1437-1443.1969
更新日期:1969-03-01 00:00:00
abstract::Of 16 restriction endonucleases known to hydrolyze rare 6- or 8-base recognition sequences that were tested, only SpeI, NotI, AscI, and SfiI generated fragments of chromosomal DNA from Yersinia pestis, the causative agent of bubonic plague, of sufficient length to permit physical analysis by pulsed-field gel electroph...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.7.2078-2086.1992
更新日期:1992-04-01 00:00:00
abstract::Flagellar phase variation of Salmonella is a phenomenon where two flagellin genes, fliC (phase 1) and fljB (phase 2), are expressed alternately. This is controlled by the inversion of a DNA segment containing the promoter for the fljB gene. The fljB gene constitutes an operon with the fljA gene, which encodes a negati...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.3.958-967.2006
更新日期:2006-02-01 00:00:00
abstract::Bacteria have evolved multiple strategies to sense and rapidly adapt to challenging and ever-changing environmental conditions. The ability to alter membrane lipid composition, a key component of the cellular envelope, is crucial for bacterial survival and adaptation in response to environmental stress. However, the p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00849-16
更新日期:2017-06-13 00:00:00
abstract::Mutations in the tcmII-tcmIV region of the Streptomyces glaucescens chromosome block the C-3 and C-8 O-methylations of the polyketide antibiotic tetracenomycin C (Tcm C). The nucleotide sequence of this region reveals the presence of two genes, tcmN and tcmO, whose deduced protein products display similarity to the hy...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.6.1810-1820.1992
更新日期:1992-03-01 00:00:00
abstract::Quorum sensing control mediated by N-acyl homoserine lactone (AHL) signaling molecules has been established as a key feature of the regulation of exoenzyme production in many gram-negative bacteria. In Chromobacterium violaceum ATCC 31532 a number of phenotypic characteristics, including production of the purple pigme...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.17.4435-4441.1998
更新日期:1998-09-01 00:00:00
abstract::Polyamines are required for rapid phospholipid turnover in Escherichia coli growing in media of low osmolarity. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.116.3.1479-1481.1973
更新日期:1973-12-01 00:00:00
abstract::A simple three-step procedure is described which yields electrophoretically homogeneous preparations of ribitol-5-phosphate dehydrogenase and xylitol-5-phosphate dehydrogenase. The former enzyme is a 115,000-molecular-weight protein composed of two subunits of identical size and is specific for its substrate, ribitol....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.4.1651-1655.1987
更新日期:1987-04-01 00:00:00
abstract::The importance of the physiological state of a culture of Saccharomyces cerevisiae for tolerance to sudden osmotic dehydration was studied, and it was investigated whether specific osmotolerance factors were demonstrable. The microcalorimeter was used to monitor growth, and different physiological states of the cultur...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.10.4562-4568.1988
更新日期:1988-10-01 00:00:00
abstract::Mutations in two chromosomal genes of Escherichia coli, cpxA and cpxB, produced a temperature-sensitive growth defect that was remedied specifically by the addition of isoleucine and valine to the minimal medium. This auxotrophy was manifested only when the medium contained exogenous leucine, suggesting that mutant ce...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.1.68-73.1980
更新日期:1980-10-01 00:00:00
abstract::Recombinant plasmid pNP5, consisting of plasmid pMB9 on which the uvrB gene is cloned, fully complements for the defects due to chromosomal uvrB mutations in the presence of the amber suppressor sup-6 or supF. Correndonuclease II activity was also completely restored in in UvrB strains containing both plasmid pNP5 and...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.1.48-53.1979
更新日期:1979-07-01 00:00:00
abstract::In aquatic environments, Caulobacter spp. can be found at the boundary between liquid and air known as the neuston. I report an approach to study temporal features of Caulobacter crescentus colonization and pellicle biofilm development at the air-liquid interface and have defined the role of cell surface structures in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00064-19
更新日期:2019-08-22 00:00:00
abstract::Genetic exchange mechanisms, to our knowledge, have not been reported for Legionella pneumophila, and consequently, studies on the genetic organization of L. pneumophila have not appeared in the literature. Here, we describe gene transfer mediated by broad host range conjugative plasmids in Legionella spp. Escherichia...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.161.1.80-84.1985
更新日期:1985-01-01 00:00:00
abstract::Mycoplasma penetrans is a newly identified species of the genus MYCOPLASMA: It was first isolated from a urine sample from a human immunodeficiency virus (HIV)-infected patient. M. penetrans changes its surface antigen profile with high frequency. The changes originate from ON<==>OFF phase variations of the P35 family...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.1.231-242.2003
更新日期:2003-01-01 00:00:00
abstract::The direction of chromosome replication in a temperature-sensitive initiation mutant of Escherichia coli (CT28) is shown autoradiographically to be bidirectional. This mode of replication persists even when the rate of replication is reduced by slow growth in succinate minimal medium or in the presence of chlorampheni...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.1.346-352.1976
更新日期:1976-01-01 00:00:00
abstract::During development of the symbiotic soil bacterium Sinorhizobium meliloti into nitrogen-fixing bacteroids, DNA replication and cell division cease and the cells undergo profound metabolic and morphological changes. Regulatory genes controlling the early stages of this process have not been identified. As a first step ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.10.3204-3210.2001
更新日期:2001-05-01 00:00:00
abstract::Sneathia amnii is a poorly characterized emerging pathogen that has been implicated in amnionitis and urethritis. We found that S. amnii damages fetal membranes, and we identified and purified a cytotoxic exotoxin that lyses human red blood cells and damages cells from fetal membranes. The gene appears to be cotranscr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00162-20
更新日期:2020-06-09 00:00:00