The ABC-type multidrug resistance transporter LmrCD is responsible for an extrusion-based mechanism of bile acid resistance in Lactococcus lactis.

Abstract:

:Upon prolonged exposure to cholate and other toxic compounds, Lactococcus lactis develops a multidrug resistance phenotype that has been attributed to an elevated expression of the heterodimeric ABC-type multidrug transporter LmrCD. To investigate the molecular basis of bile acid resistance in L. lactis and to evaluate the contribution of efflux-based mechanisms in this process, the drug-sensitive L. lactis NZ9000 DeltalmrCD strain was challenged with cholate. A resistant strain was obtained that, compared to the parental strain, showed (i) significantly improved resistance toward several bile acids but not to drugs, (ii) morphological changes, and (iii) an altered susceptibility to antimicrobial peptides. Transcriptome and transport analyses suggest that the acquired resistance is unrelated to elevated transport activity but, instead, results from a multitude of stress responses, changes to the cell envelope, and metabolic changes. In contrast, wild-type cells induce the expression of lmrCD upon exposure to cholate, whereupon the cholate is actively extruded from the cells. Together, these data suggest a central role for an efflux-based mechanism in bile acid resistance and implicate LmrCD as the main system responsible in L. lactis.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Zaidi AH,Bakkes PJ,Lubelski J,Agustiandari H,Kuipers OP,Driessen AJ

doi

10.1128/JB.00485-08

subject

Has Abstract

pub_date

2008-11-01 00:00:00

pages

7357-66

issue

22

eissn

0021-9193

issn

1098-5530

pii

JB.00485-08

journal_volume

190

pub_type

杂志文章
  • EFFECT OF TETRACYCLINES ON THE INTRACELLULAR AMINO ACIDS OF MOLDS.

    abstract::Freeman, Bob A. (University of Chicago, Chicago, Ill.) and Richard Circo. Effect of tetracyclines on the intracellular amino acids of molds. J. Bacteriol. 86:38-44. 1963.-The tetracycline antibiotics were shown to alter the amino acid metabolism of molds whose growth is not markedly affected. Eight molds were grown in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.86.1.38-44.1963

    authors: FREEMAN BA,CIRCO R

    更新日期:1963-07-01 00:00:00

  • Citrate transport system of Streptococcus diacetilactis.

    abstract::Harvey, R. J. (University of California, Davis) and E. B. Collins. Citrate transport system of Streptococcus diacetilactis. J. Bacteriol. 83:1005-1009. 1962.-The uptake of citrate by Streptococcus diacetilactis is mediated by a transport system that was distinguished from passive diffusion by inducibility and kinetics...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.5.1005-1009.1962

    authors: HARVEY RJ,COLLINS EB

    更新日期:1962-05-01 00:00:00

  • Deoxyribonucleic acid relatedness among species of Erwinia and between Erwinia species and other enterobacteria.

    abstract::Relatedness in species of Erwinia was assessed by determining the extent of reassociation in heterologous deoxyribonucleic acid preparations. Thermal elution chromatography on hydroxyapatite was used to separate reassociated nucleotide sequences from nonreassociated sequences and to determine the thermal stability of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.110.1.12-17.1972

    authors: Brenner DJ,Fanning GR,Steigerwalt AG

    更新日期:1972-04-01 00:00:00

  • Low-frequency, pbsi-mediated plasmid transduction in Bacillus pumilus.

    abstract::Three bacteriophages were tested for ability to transduce the plasmid of pPL10 between W mutant derivatives of Bacillus pumilus NRS 576. Phage PBP1- and PMB1-generated plasmid transductants occurred at about 10% the frequency of transductants for a chromosome marker. Phage PBS1-generated plasmid transductants occurred...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.2.829-831.1976

    authors: Bramucci MG,Lovett PS

    更新日期:1976-08-01 00:00:00

  • Neutralization of endotoxin toxicity in chick embryos by antibiotics.

    abstract::Rifkind, David (University of Colorado Medical Center, Denver), and John D. Palmer. Neutralization of endotoxin toxicity in chick embryos by antibiotics. J. Bacteriol. 92:815-819. 1966.-Three cationic cyclic polypeptide antibiotics, polymyxin B sulfate colistin sulfate, and tyrocidine hydrochloride, were shown to neut...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.92.4.815-819.1966

    authors: Rifkind D,Palmer JD

    更新日期:1966-10-01 00:00:00

  • Complete genome sequence of Rickettsia heilongjiangensis, an emerging tick-transmitted human pathogen.

    abstract::Rickettsia heilongjiangensis is an emerging tick-transmitted human pathogen causing far-Eastern spotted fever. Here we report the complete sequence and the main features of the genome of R. heilongjiangensis (strain 054). ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05852-11

    authors: Duan C,Tong Y,Huang Y,Wang X,Xiong X,Wen B

    更新日期:2011-10-01 00:00:00

  • Identification of the lipopolysaccharide core region as the receptor site for a cytotoxin-converting phage, phi CTX, of Pseudomonas aeruginosa.

    abstract::A temperate phage, phi CTX, is a cytotoxin-converting phage of Pseudomonas aeruginosa. In this study, we characterized the lipopolysaccharide (LPS) structures of phi CTX-resistant mutants derived from phi CTX-sensitive strains. phi CTX infectivity was neutralized by LPS preparations derived from sensitive strains but ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.17.5262-5269.1994

    authors: Yokota S,Hayashi T,Matsumoto H

    更新日期:1994-09-01 00:00:00

  • Regulatory elements of the raffinose operon: nucleotide sequences of operator and repressor genes.

    abstract::The raffinose (raf) operon is negatively controlled by the specific binding of raf repressor (rafR gene) to raf operator (rafO) DNA. Both rafR and rafO have been sequenced. The 1,011-base-pair rafR gene encodes a 336-amino-acid polypeptide containing an N-terminal helix-turn-helix motif. rafO, as defined by in vivo ti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.4.2178-2180.1990

    authors: Aslanidis C,Schmitt R

    更新日期:1990-04-01 00:00:00

  • Factors Affecting Formation of Incomplete Vi Antibody in Mice.

    abstract::Gaines, Sidney (Walter Reed Army Institute of Research, Washington, D.C.), Julius A. Currie, and Joseph G. Tully. Factors affecting formation of incomplete Vi antibody in mice. J. Bacteriol. 90:635-642. 1965.-Single immunizing doses of purified Vi antigen elicited complete and incomplete Vi antibodies in BALB/c mice, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.3.635-642.1965

    authors: Gaines S,Currie JA,Tully JG

    更新日期:1965-09-01 00:00:00

  • Analysis of the σE regulon in Crohn's disease-associated Escherichia coli revealed involvement of the waaWVL operon in biofilm formation.

    abstract:UNLABELLED:Ileal lesions of patients with Crohn's disease are colonized by adherent-invasive Escherichia coli (AIEC), which is able to adhere to and to invade intestinal epithelial cells (IEC), to replicate within macrophages, and to form biofilms on the surface of the intestinal mucosa. Previous analyses indicated the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02499-14

    authors: Chassaing B,Garénaux E,Carriere J,Rolhion N,Guérardel Y,Barnich N,Bonnet R,Darfeuille-Michaud A

    更新日期:2015-04-01 00:00:00

  • Evidence that the clindamycin-erythromycin resistance gene of Bacteroides plasmid pBF4 is on a transposable element.

    abstract::We constructed a shuttle vector, pE5-2, which can replicate in both Bacteroides spp. and Escherichia coli. pE5-2 contains a cryptic Bacteroides plasmid (pB8-51), a 3.8-kilobase (kb) EcoRI-D fragment from the 41-kb Bacteroides fragilis plasmid pBF4, and RSF1010, an IncQ E. coli plasmid. pE5-2 was mobilized by R751, an ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.2.626-632.1985

    authors: Shoemaker NB,Guthrie EP,Salyers AA,Gardner JF

    更新日期:1985-05-01 00:00:00

  • Levels of small molecules and enzymes in the mother cell compartment and the forespore of sporulating Bacillus megaterium.

    abstract::We have determined the amounts of a number of small molecules and enzymes in the mother cell compartment and the developing forespore during sporulation of Bacillus megaterium. Significant amounts of adenosine 5'-triphosphate and reduced nicotinamide adenine dinucleotide were present in the forespore compartment befor...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.3.1130-1138.1977

    authors: Singh RP,Setlow B,Setlow P

    更新日期:1977-06-01 00:00:00

  • Core and panmetabolism in Escherichia coli.

    abstract::Escherichia coli exhibits a wide range of lifestyles encompassing commensalism and various pathogenic behaviors which its highly dynamic genome contributes to develop. How environmental and host factors shape the genetic structure of E. coli strains remains, however, largely unknown. Following a previous study of E. c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01192-10

    authors: Vieira G,Sabarly V,Bourguignon PY,Durot M,Le Fèvre F,Mornico D,Vallenet D,Bouvet O,Denamur E,Schachter V,Médigue C

    更新日期:2011-03-01 00:00:00

  • Staphylococcus aureus YoeB homologues inhibit translation initiation.

    abstract::YoeB is a bacterial toxin encoded by the yefM-yoeB toxin-antitoxin system found in various bacterial genomes. Here, we show that Staphylococcus aureus contains two YoeB homologues, both of which function as ribosome-dependent mRNA interferases to inhibit translation initiation in a manner identical to that of YoeB-ec ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00623-09

    authors: Yoshizumi S,Zhang Y,Yamaguchi Y,Chen L,Kreiswirth BN,Inouye M

    更新日期:2009-09-01 00:00:00

  • Construction and characterization of an Escherichia coli strain with a uncI mutation.

    abstract::A strain of Escherichia coli with a mutation in the promoter proximal gene ( uncI ) of the unc operon has been constructed by using a new gene replacement method. The mutation is a deletion of a defined sequence of 196 base pairs. It was constructed by homologous integration and segregation of a ColE1-derived recombin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.158.3.820-825.1984

    authors: Gay NJ

    更新日期:1984-06-01 00:00:00

  • Targeting the HUβ Protein Prevents Porphyromonas gingivalis from Entering into Preexisting Biofilms.

    abstract::The oral cavity is home to a wide variety of bacterial species, both commensal, such as various streptococcal species, and pathogenic, such as Porphyromonas gingivalis, one of the main etiological agents of periodontal disease. Our understanding of how these bacteria ultimately cause disease is highly dependent upon u...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00790-17

    authors: Rocco CJ,Bakaletz LO,Goodman SD

    更新日期:2018-05-09 00:00:00

  • A novel apical corpuscle in hypahe of Mucor rouxii.

    abstract::Median longitudinal sections of germ tube apices of Mucor rouxii revealed the presence of a single, roughly hemispherical, electron-dense organelle, in intimate contact with the apical cell wall. Conceivably, this "apical corpuscle" may be responsible for the emission of the germ tube and its continued apical growth. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.6.2399-2402.1968

    authors: Bartnicki-Garcia S,Nelson N,Cota-Robles E

    更新日期:1968-06-01 00:00:00

  • Reconstitution of an active lactose carrier in vivo by simultaneous synthesis of two complementary protein fragments.

    abstract::Escherichia coli lactose permease mediates the proton-driven translocation of galactosides across the cytoplasmic membrane. To define regions important for membrane insertion as well as for biological function, we constructed plasmids encoding different portions of the lactose carrier. Among several lacY deletions, tw...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.9.5374-5381.1990

    authors: Wrubel W,Stochaj U,Sonnewald U,Theres C,Ehring R

    更新日期:1990-09-01 00:00:00

  • Mapping of genes involved in macromolecular synthesis on the chromosome of Streptomyces coelicolor A3(2).

    abstract::The genes for the beta, beta', and seven sigma factor subunits of RNA polymerase, for elongation factors EF-Tu1 and EF-Tu3, and for six rRNA operons were mapped on the combined genetic and physical map of the Streptomyces coelicolor chromosome. Like the previously mapped tRNA genes, the RNA polymerase and rRNA genes m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.2.473-476.1995

    authors: van Wezel GP,Buttner MJ,Vijgenboom E,Bosch L,Hopwood DA,Kieser HM

    更新日期:1995-01-01 00:00:00

  • Genetic dissection of T4 lysis.

    abstract::t is the holin gene for coliphage T4, encoding a 218-amino-acid (aa) protein essential for the inner membrane hole formation that initiates lysis and terminates the phage infection cycle. T is predicted to be an integral membrane protein that adopts an N(in)-C(out) topology with a single transmembrane domain (TMD). Th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01548-14

    authors: Moussa SH,Lawler JL,Young R

    更新日期:2014-06-01 00:00:00

  • Induction of the autolytic system of Escherichia coli by specific insertion of bacteriophage MS2 lysis protein into the bacterial cell envelope.

    abstract::Bacterial lysis induced by the expression of the cloned lysis gene of the RNA bacteriophage MS2 in Escherichia coli was shown to be under the same regulatory control mechanisms as penicillin-induced lysis. It was controlled by the stringent response and showed the phenomenon of tolerance when E. coli was grown at pH 5...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.11.5027-5033.1988

    authors: Walderich B,Ursinus-Wössner A,van Duin J,Höltje JV

    更新日期:1988-11-01 00:00:00

  • Difference between manganese ion requirements of pediococci and enterococci.

    abstract::For maximal turbidity and dissimilatory activity, three strains of pediococci required the addition of Mn(2+) to Rogosa basal medium. Twenty-two strains of enterococci proved indifferent to this supplement. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.1.627-628.1972

    authors: Efthymiou CJ,Joseph SW

    更新日期:1972-10-01 00:00:00

  • An epitope of elongation factor Tu is widely distributed within the bacterial and archaeal domains.

    abstract::A monoclonal antibody (MAb), MAb 900, which detects a 43-kDa protein present on Escherichia coli was found. Subsequently, more than 90 organisms, belonging to either the bacterial, archaeal, or eucaryal domain, were tested for reactivity to this MAb. Of the bacterial and archaeal domains, almost all species proved to ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.1.11-19.1995

    authors: Weber S,Lottspeich F,Köhl J

    更新日期:1995-01-01 00:00:00

  • Allantoate transport in Saccharomyces cerevisiae.

    abstract::Allantoate uptake appears to be mediated by an energy-dependent active transport system with an apparent Michaelis constant of about 50 microM. Cells were able to accumulate allantoate to greater than 3,000 times the extracellular concentration. The rate of accumulation was maximum at pH 5.7 to 5.8. The energy source ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.3.971-979.1979

    authors: Turoscy V,Cooper TG

    更新日期:1979-12-01 00:00:00

  • Relationships between fatty acid and polyketide synthases from Streptomyces coelicolor A3(2): characterization of the fatty acid synthase acyl carrier protein.

    abstract::We have characterized an acyl carrier protein (ACP) presumed to be involved in the synthesis of fatty acids in Streptomyces coelicolor A3(2). This is the third ACP to have been identified in S. coelicolor; the two previously characterized ACPs are involved in the synthesis of two aromatic polyketides: the blue-pigment...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.19.5660-5667.1996

    authors: Revill WP,Bibb MJ,Hopwood DA

    更新日期:1996-10-01 00:00:00

  • Protein difference between competent and noncompetent cultures of a group H Streptococcus.

    abstract::Acidified phenol extracts prepared from competent cultures of a group H Streptococcus strain Wicky made competent with competence factor derived from cultures of another group H Streptococcus, strain Challis, showed a difference in polyacrylamide-gel protein patterns when compared to extracts prepared from noncompeten...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.1.360-362.1970

    authors: Ranhand JM,Theodore TS,Cole RM

    更新日期:1970-10-01 00:00:00

  • Inactivation of Serpulina hyodysenteriae flaA1 and flaB1 periplasmic flagellar genes by electroporation-mediated allelic exchange.

    abstract::Serpulina hyodysenteriae, the etiologic agent of swine dysentery, contains complex periplasmic flagella which are composed of multiple class A and class B polypeptides. To examine the role these proteins play in flagellar synthesis, structure, and function and to develop strains which may provide insight into the impo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.20.5959-5970.1995

    authors: Rosey EL,Kennedy MJ,Petrella DK,Ulrich RG,Yancey RJ Jr

    更新日期:1995-10-01 00:00:00

  • Three-dimensional structure of the nickel-containing hydrogenase from Thiocapsa roseopersicina.

    abstract::The three-dimensional structure of the nickel-containing hydrogenase from Thiocapsa roseopersicina has been determined at a resolution of 2 nm in the plane and 4 nm in the vertical direction by electron microscopy and computerized image processing on microcrystals of the enzyme. The enzyme forms a large ring-shaped co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.8.2576-2580.1991

    authors: Sherman MB,Orlova EV,Smirnova EA,Hovmöller S,Zorin NA

    更新日期:1991-04-01 00:00:00

  • Identification and characterization of genes determining receptor binding and pilus length of Escherichia coli type 1 pili.

    abstract::We describe the identification and characterization of two genes and their gene products responsible for determining receptor binding and pilus length in type 1-piliated Escherichia coli. One gene, pilE, conferred the ability of piliated cells to agglutinate guinea pig erythrocytes. The other gene, pilF, determined pi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.2.640-645.1987

    authors: Maurer L,Orndorff PE

    更新日期:1987-02-01 00:00:00

  • Properties of an ordered ring structure formed by recombinant Treponema pallidum surface antigen 4D.

    abstract::Ultrastructural and biochemical studies of a recombinant Treponema pallidum surface antigen designated 4D have been conducted due to its likely biological significance. Electron microscopy demonstrated that the 190-kilodalton (kDa) 4D molecule is an ordered ring structure of 10-nm diameter. The 90-kDa proteinase K-tre...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.165.3.732-739.1986

    authors: Fehniger TE,Radolf JD,Lovett MA

    更新日期:1986-03-01 00:00:00