Abstract:
:Upon prolonged exposure to cholate and other toxic compounds, Lactococcus lactis develops a multidrug resistance phenotype that has been attributed to an elevated expression of the heterodimeric ABC-type multidrug transporter LmrCD. To investigate the molecular basis of bile acid resistance in L. lactis and to evaluate the contribution of efflux-based mechanisms in this process, the drug-sensitive L. lactis NZ9000 DeltalmrCD strain was challenged with cholate. A resistant strain was obtained that, compared to the parental strain, showed (i) significantly improved resistance toward several bile acids but not to drugs, (ii) morphological changes, and (iii) an altered susceptibility to antimicrobial peptides. Transcriptome and transport analyses suggest that the acquired resistance is unrelated to elevated transport activity but, instead, results from a multitude of stress responses, changes to the cell envelope, and metabolic changes. In contrast, wild-type cells induce the expression of lmrCD upon exposure to cholate, whereupon the cholate is actively extruded from the cells. Together, these data suggest a central role for an efflux-based mechanism in bile acid resistance and implicate LmrCD as the main system responsible in L. lactis.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Zaidi AH,Bakkes PJ,Lubelski J,Agustiandari H,Kuipers OP,Driessen AJdoi
10.1128/JB.00485-08subject
Has Abstractpub_date
2008-11-01 00:00:00pages
7357-66issue
22eissn
0021-9193issn
1098-5530pii
JB.00485-08journal_volume
190pub_type
杂志文章abstract::Freeman, Bob A. (University of Chicago, Chicago, Ill.) and Richard Circo. Effect of tetracyclines on the intracellular amino acids of molds. J. Bacteriol. 86:38-44. 1963.-The tetracycline antibiotics were shown to alter the amino acid metabolism of molds whose growth is not markedly affected. Eight molds were grown in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.1.38-44.1963
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更新日期:1994-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1990-04-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JB.162.2.626-632.1985
更新日期:1985-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.3.1130-1138.1977
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pub_type: 杂志文章
doi:10.1128/JB.158.3.820-825.1984
更新日期:1984-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00790-17
更新日期:2018-05-09 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.6.2399-2402.1968
更新日期:1968-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1990-09-01 00:00:00
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更新日期:1988-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1972-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1995-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.140.3.971-979.1979
更新日期:1979-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1996-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1970-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1991-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1987-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1986-03-01 00:00:00