Relationships between fatty acid and polyketide synthases from Streptomyces coelicolor A3(2): characterization of the fatty acid synthase acyl carrier protein.

abstract：:Evolution by natural selection occurs in cultures of Escherichia coli maintained under carbon starvation stress. Mutants of increased fitness express a growth advantage in stationary phase (GASP) phenotype, enabling them to grow and displace the parent as the majority population. The first GASP mutation was identified...

journal_title：Journal of bacteriology

authors： Zinser ER,Kolter R

更新日期：2000-08-01 00:00:00

abstract：:2-Carboxybenzaldehyde dehydrogenase from the phenanthrene-degrading bacterium Nocardioides sp. strain KP7 was purified and characterized. The purified enzyme had a molecular mass of 53 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 205 kDa by gel filtration chromatography. Thus, the homotetramer ...

journal_title：Journal of bacteriology

authors： Iwabuchi T,Harayama S

更新日期：1997-10-01 00:00:00

abstract：:pBL2 was identified genetically but not physically in Streptomyces lividans after its mating with S. bambergiensis. During conjugation, pBL2 was transferred at high frequency to S. lividans and S. coelicolor. pBL2.1 DNA isolated from S. coelicolor exconjugants as a circular plasmid was shown to derive from the genome ...

journal_title：Journal of bacteriology

authors： Zotchev SB,Schrempf H,Hutchinson CR

更新日期：1995-08-01 00:00:00

abstract：:The host range of an octopine Ti plasmid is limited to Rhizobiaceae. This has been extended also to Escherichia coli in the form of a stable cointegrate with the wide-host-range plasmid R772. Its structure was studied by constructing a physical map of R772 and of the R772::pTiB6 cointegrate. An insertion sequence pres...

journal_title：Journal of bacteriology

authors： Hille J,van Kan J,Klasen I,Schilperoort R

更新日期：1983-05-01 00:00:00

abstract：:Previously, we demonstrated that Escherichia coli tolC mutations reduce the high-level resistance to tetracycline afforded by the transposon Tn10-encoded TetA pump from resistance at 200 microg/ml to resistance at 40 microg/ml. In this study, we found that the addition of an sbmA mutation to a tolC::Tn10 mutant exacer...

journal_title：Journal of bacteriology

authors： de Cristóbal RE,Vincent PA,Salomón RA

更新日期：2008-02-01 00:00:00

abstract：:We have used a plasmid-based transcriptional reporter system to examine the transcriptional effects of 33 single point mutations in the box A region (TATA-like sequence) of the Haloferax volcanii tRNA(Lys) promoter. The most pronounced effects on transcriptional efficiency were found when the nucleotides corresponding...

journal_title：Journal of bacteriology

authors： Palmer JR,Daniels CJ

更新日期：1995-04-01 00:00:00

abstract：:The conjugation system of the IncP alpha plasmid RK2/RP4 is encoded by transfer regions designated Tra1, Tra2, and Tra3. The Tra1 core region, cloned on plasmid pDG4 delta 22, consists of the origin of transfer (oriT) and 2.6 kilobases of flanking DNA providing IncP alpha plasmid-specific functions that allow pDG4 del...

journal_title：Journal of bacteriology

authors： Guiney DG,Deiss C,Simnad V,Yee L,Pansegrau W,Lanka E

更新日期：1989-07-01 00:00:00

abstract：:Methylation of DNA at the C-5 position of cytosine occurs in diverse organisms. This modification can increase the rate of C→T transitions at the methylated position. In Escherichia coli and related enteric bacteria, the inner C residues of the sequence CCWGG (W is A or T) are methylated by the Dcm enzyme. These sites...

journal_title：Journal of bacteriology

authors： Cherry JL

更新日期：2018-11-26 00:00:00

abstract：:The force required to rupture bonds between individual Staphylococcus aureus MSCRAMMs and surfaces coated with extracellular matrix molecules has been quantified by using optical tweezers. The observed binding forces between fibrinogen or fibronectin and S. aureus MSCRAMMs occurred as an approximate integer multiple o...

journal_title：Journal of bacteriology

authors： Simpson KH,Bowden G,Höök M,Anvari B

更新日期：2003-03-01 00:00:00

abstract：:Vi-agglutinin, active cutaneous anaphylaxis and protective responses (ed(50)) of three mouse strains (CFW, NIH, and Balb/cAnN) to acetone-inactivated typhoid vaccine and soluble Vi antigen were compared. Seven days after immunization with either typhoid vaccine or Vi antigen the three strains of mice differed with res...

journal_title：Journal of bacteriology

authors： Esposito VM,Feeley JC,Leeder WD,Pittman M

更新日期：1969-07-01 00:00:00

abstract：:Functional domains in the RepI replication initiator protein have been identified by classical and site-directed mutagenesis techniques. Mutations conferring an increase in plasmid copy number contained alterations in a key position of a putative helix-turn-helix DNA binding motif. The mutations did not appear to affe...

journal_title：Journal of bacteriology

authors： Gammie AE,Tolmasky ME,Crosa JH

更新日期：1993-06-01 00:00:00

abstract：:In a previous study, we showed that in bacteroids, transcription of the inducible nod genes does not occur and expression of nodD is decreased by 65% (H. R. M. Schlaman, B. Horvath, E. Vijgenboom, R.J.H. Okker, and B. J. J. Lugtenberg, J. Bacteriol. 173:4277-4287, 1991). In the present study, we show, using gel retard...

journal_title：Journal of bacteriology

authors： Schlaman HR,Lugtenberg BJ,Okker RJ

更新日期：1992-10-01 00:00:00

abstract：:Eleven conditional lethal dnaG(Ts) mutations were located by chemical cleavage of heteroduplexes formed between polymerase chain reaction-amplified DNAs from wild-type and mutant dnaG genes. This entailed end labeling one DNA strand of the heteroduplex, chemically modifying the strands with hydroxylamine or osmium tet...

journal_title：Journal of bacteriology

authors： Grompe M,Versalovic J,Koeuth T,Lupski JR

更新日期：1991-02-01 00:00:00

abstract：UNLABELLED:During biosynthesis of [NiFe]-hydrogenase 2 (Hyd-2) of Escherichia coli, a 15-amino-acid C-terminal peptide is cleaved from the catalytic large subunit precursor, pro-HybC. This peptide is removed only after NiFe(CN)2CO cofactor insertion by the Hyp accessory protein machinery has been completed, suggesting ...

journal_title：Journal of bacteriology

authors： Thomas C,Muhr E,Sawers RG

更新日期：2015-09-01 00:00:00

abstract：:We isolated pili from Escherichia coli SS142. These pili had a diameter of 6 nm and an average length of 400 nm. They were composed of subunits with a molecular weight of 18,000. Their amino acid composition was determined; methionine and proline were not detected. The isolated pili retained mannose-resistant hemagglu...

journal_title：Journal of bacteriology

authors： Mett H,Kloetzlen L,Vosbeck K

更新日期：1983-02-01 00:00:00

abstract：:Oligotropha carboxidovorans OM5(T) (DSM 1227, ATCC 49405) is a chemolithoautotrophic bacterium with the capability to utilize carbon monoxide, carbon dioxide, and hydrogen. It is also capable of heterotrophic growth under appropriate environmental conditions. Here we report the annotated genome sequence of the circula...

journal_title：Journal of bacteriology

authors： Paul D,Bridges S,Burgess SC,Dandass Y,Lawrence ML

更新日期：2008-08-01 00:00:00

abstract：:Chromosomal DNAs from exponential-phase and competent cells of Haemophilus influenzae were examined by electron microscopy to determine whether the chromosome undergoes structural changes during competence development. Single-stranded gaps and single-stranded tails formed in chromosomal DNA during competence developme...

journal_title：Journal of bacteriology

authors： McCarthy D,Kupfer DM

更新日期：1987-02-01 00:00:00

abstract：:Mycoplasma laidlawii was recovered from burns from 13 of 52 patients. In most cases, cocci or bacilli were isolated simultaneously. ...

journal_title：Journal of bacteriology

authors： Markham JG,Markham NP

更新日期：1969-05-01 00:00:00

abstract：:Cells of Ureaplasma urealyticum that were prepared by a ruthenium red technique demonstrated an extramembranous layer of polyanions of about 15 to 30 nm in width. Application of the concanavalin A-iron dextran strain indicated that the outer surface of this layer contained glucosyl-like residues. ...

journal_title：Journal of bacteriology

authors： Robertson J,Smook E

更新日期：1976-11-01 00:00:00

abstract：:Klebsiella pneumoniae porin genes were analyzed to detect mutations accounting for the porin deficiency observed in many beta-lactam-resistant strains. PCR and Southern blot analysis revealed the existence of a third porin gene in addition to the OmpK36 and OmpK35 porin genes previously described. This new porin gene ...

journal_title：Journal of bacteriology

authors： Doménech-Sánchez A,Hernández-Allés S,Martínez-Martínez L,Benedí VJ,Albertí S

更新日期：1999-05-01 00:00:00

abstract：:The butyrogenic genes from Clostridium difficile DSM 1296(T) have been cloned and expressed in Escherichia coli. The enzymes acetyl-coenzyme A (CoA) C-acetyltransferase, 3-hydroxybutyryl-CoA dehydrogenase, crotonase, phosphate butyryltransferase, and butyrate kinase and the butyryl-CoA dehydrogenase complex composed o...

journal_title：Journal of bacteriology

authors： Aboulnaga el-H,Pinkenburg O,Schiffels J,El-Refai A,Buckel W,Selmer T

更新日期：2013-08-01 00:00:00

abstract：:We previously reported that group D streptococci exhibited immunoglobulin G (IgG)-binding activity and that a 52-kDa IgG-binding protein was present in all Streptococcus suis strains examined (B. Serhir, R. Higgins, B. Foiry, and M. Jacques, J. Gen. Microbiol. 139:2953-2958, 1993). The objective of the present study w...

journal_title：Journal of bacteriology

authors： Serhir B,Dubreuil D,Higgins R,Jacques M

更新日期：1995-07-01 00:00:00

abstract：:Leuconostoc mesenteroides subsp. mesenteroides is one of the most predominant lactic acid bacterial groups during kimchi fermentation. Here, we report the complete genome sequence of L. mesenteroides subsp. mesenteroides J18, which was isolated from kimchi. The genome of the strain consists of a 1,896,561-bp chromosom...

journal_title：Journal of bacteriology

authors： Jung JY,Lee SH,Lee SH,Jeon CO

更新日期：2012-02-01 00:00:00

abstract：:Carbon catabolite repression (CCR) allows bacteria to alter metabolism in response to the availability of specific sugar sources, and increasing evidence suggests that CCR is involved in regulating virulence gene expression in many pathogens. A scan of the M1 SF370 group A streptococcus (GAS) genome using a Bacillus s...

journal_title：Journal of bacteriology

authors： Almengor AC,Kinkel TL,Day SJ,McIver KS

更新日期：2007-12-01 00:00:00

abstract：:Microbacterium yannicii is a Gram-positive, aerobic, yellow-pigmented, rod-shaped, nonmotile, oxidase-negative, and catalase-positive bacterium isolated on Columbia colistin-nalidixic acid (CNA) agar with 5% sheep blood from the sputum of a cystic fibrosis patient. The present study reports the draft genome of a Micro...

journal_title：Journal of bacteriology

authors： Sharma P,Diene SM,Gimenez G,Robert C,Rolain JM

更新日期：2012-09-01 00:00:00

abstract：:Inhibition of sterol uptake in Saccharomyces cerevisiae sterol auxotroph FY3 (alpha hem1 erg7 ura) by delta-aminolevulinic acid (ALA) is dependent on the ability of the organism to synthesize heme from ALA. Sterol-depleted cells not exposed to ALA or strain PFY3 cells, with a double heme mutation, exposed to ALA did n...

journal_title：Journal of bacteriology

authors： Lorenz RT,Parks LW

更新日期：1987-08-01 00:00:00

abstract：:The antibacterial protein Colicin V (ColV) is secreted from gram-negative bacteria by a signal sequence-independent pathway. The proteins that mediate the export of ColV share sequence similarities with components from other signal sequence-independent export systems such as those for alpha-hemolysin (Hly) and Erwinia...

journal_title：Journal of bacteriology

authors： Fath MJ,Skvirsky RC,Kolter R

更新日期：1991-12-01 00:00:00

abstract：:Six analogues of amino acids killed corresponding auxotrophs of Escherichia coli. With all but one analogue, protein synthesis was required for lethality. ...

journal_title：Journal of bacteriology

authors： Rabinovitz M,Finkleman A,Reagan RL,Breitman TR

更新日期：1969-07-01 00:00:00

abstract：:The ompA gene of Escherichia coli codes for a major protein of the outer membrane. When this gene was moved between various unrelated strains (E. coli K-12 and two clinical isolates of E. coli) by transduction, the gene was expressed very poorly. Recombinants carrying "foreign" genes produced no OmpA protein which cou...

journal_title：Journal of bacteriology

authors： Beher M,Pugsley A,Schnaitman C

更新日期：1980-07-01 00:00:00

abstract：:A fatty acid Delta(9)-desaturase mutant of yeast was analyzed to establish the specificity of the fatty acid structural requirement. Several double-bond-containing and substituted fatty acids were tested. It was concluded that the presence of a Delta(9)cis double bond is necessary for growth. The need for a specific c...

journal_title：Journal of bacteriology

authors： Wisnieski BJ,Keith AD,Resnick MR

更新日期：1970-01-01 00:00:00