Abstract:
:Serpulina hyodysenteriae, the etiologic agent of swine dysentery, contains complex periplasmic flagella which are composed of multiple class A and class B polypeptides. To examine the role these proteins play in flagellar synthesis, structure, and function and to develop strains which may provide insight into the importance of motility in the etiology of this pathogen, we constructed specific periplasmic flagellar mutations in S. hyodysenteriae B204. The cloned flaA1 and flaB1 genes were disrupted by replacement of internal fragments with chloramphenicol and/or kanamycin gene cassettes. Following delivery of these suicide plasmids into S. hyodysenteriae, homologous recombination and allelic exchange at the targeted chromosomal flaA1 and flaB1 genes was verified by PCR, sequence, and Southern analysis. The utility of a chloramphenicol resistance gene cassette for targeted gene disruption was demonstrated and found more amenable than kanamycin as a selective marker in S. hyodysenteriae. Immunoblots of cell lysates of the flagellar mutants with antiserum raised against purified FlaA or FlaB confirmed the absence of the corresponding sheath or core protein. Both mutations selectively abolished expression of the targeted gene without affecting synthesis of the other flagellar polypeptide. flaA1 and flaB1 mutant strains exhibited altered motility in vitro and were less efficient in movement through a liquid medium. Paradoxically, isogenic strains containing specifically disrupted flaA1 or flaB1 alleles were capable of assembling periplasmic flagella that were morphologically normal as evidenced by electron microscopy. This is the first report of specific inactivation of a motility-associated gene in spirochetes.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Rosey EL,Kennedy MJ,Petrella DK,Ulrich RG,Yancey RJ Jrdoi
10.1128/jb.177.20.5959-5970.1995subject
Has Abstractpub_date
1995-10-01 00:00:00pages
5959-70issue
20eissn
0021-9193issn
1098-5530journal_volume
177pub_type
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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doi:10.1128/jb.174.19.6207-6214.1992
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1976-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.127.2.904-916.1976
更新日期:1976-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1970-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.4.1184-1187.1994
更新日期:1994-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1984-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.01946-12
更新日期:2013-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.24.7449-7456.1999
更新日期:1999-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2007-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2008-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1993-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.14.4444-4447.1994
更新日期:1994-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.5.2259-2266.1990
更新日期:1990-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1971-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.8.2681-2690.1991
更新日期:1991-04-01 00:00:00
abstract::The effect of the iron content of the medium on the yields of extracellular products by seven distinct strains of Pseudomonas aeruginosa was examined. All strains showed at least an 85% decrease in toxin A yields when grown in medium containing 5.0 mug of iron per ml (high iron) as compared to 0.05 mug/ml (low iron), ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.1.193-200.1979
更新日期:1979-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.3.861-863.1995
更新日期:1995-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2014-04-01 00:00:00
abstract::An Escherichia coli F19 recA, nitrate reductase-deficient mutant was constructed by transposon mutagenesis and shown to be resistant to metronidazole. This mutant was a most suitable host for the isolation of Clostridium acetobutylicum genes on recombinant plasmids, which activated metronidazole and rendered the E. co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.3.1088-1095.1991
更新日期:1991-02-01 00:00:00
abstract::Four cold-sensitive mutants of Escherichia coli have been isolated which show a reduced ability to synthesize deoxyribonucleic acid at low temperature. The mutants also have a reduced ability to incorporate nucleoside triphosphates into deoxyribonucleic acid at low temperature in cell preparations made permeable with ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.1.99-107.1975
更新日期:1975-01-01 00:00:00
abstract::The interaction of 12 phage strains with bacilli, protoplasts, and L forms of Bacillus subtilis 168 and with eight of its mutants and two of its lysogens is described qualitatively and quantitatively. After removal of the cell wall from B. subtilis 168, 11 of the 12 phage strains can still adsorb to the protoplasts, n...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.1.445-448.1975
更新日期:1975-10-01 00:00:00