Inactivation of Serpulina hyodysenteriae flaA1 and flaB1 periplasmic flagellar genes by electroporation-mediated allelic exchange.

Abstract:

:Serpulina hyodysenteriae, the etiologic agent of swine dysentery, contains complex periplasmic flagella which are composed of multiple class A and class B polypeptides. To examine the role these proteins play in flagellar synthesis, structure, and function and to develop strains which may provide insight into the importance of motility in the etiology of this pathogen, we constructed specific periplasmic flagellar mutations in S. hyodysenteriae B204. The cloned flaA1 and flaB1 genes were disrupted by replacement of internal fragments with chloramphenicol and/or kanamycin gene cassettes. Following delivery of these suicide plasmids into S. hyodysenteriae, homologous recombination and allelic exchange at the targeted chromosomal flaA1 and flaB1 genes was verified by PCR, sequence, and Southern analysis. The utility of a chloramphenicol resistance gene cassette for targeted gene disruption was demonstrated and found more amenable than kanamycin as a selective marker in S. hyodysenteriae. Immunoblots of cell lysates of the flagellar mutants with antiserum raised against purified FlaA or FlaB confirmed the absence of the corresponding sheath or core protein. Both mutations selectively abolished expression of the targeted gene without affecting synthesis of the other flagellar polypeptide. flaA1 and flaB1 mutant strains exhibited altered motility in vitro and were less efficient in movement through a liquid medium. Paradoxically, isogenic strains containing specifically disrupted flaA1 or flaB1 alleles were capable of assembling periplasmic flagella that were morphologically normal as evidenced by electron microscopy. This is the first report of specific inactivation of a motility-associated gene in spirochetes.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Rosey EL,Kennedy MJ,Petrella DK,Ulrich RG,Yancey RJ Jr

doi

10.1128/jb.177.20.5959-5970.1995

subject

Has Abstract

pub_date

1995-10-01 00:00:00

pages

5959-70

issue

20

eissn

0021-9193

issn

1098-5530

journal_volume

177

pub_type

杂志文章
  • A HilA-independent pathway to Salmonella typhimurium invasion gene transcription.

    abstract::Salmonella typhimurium invasion of nonphagocytic cells requires the expression of a type III secretion system (TTSS) encoded within Salmonella pathogenicity island 1 (SPI1). TTSS gene transcription is activated in response to environmental signals and requires transcriptional regulators encoded within (HilA) and outsi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.10.3096-3104.1999

    authors: Rakeman JL,Bonifield HR,Miller SI

    更新日期:1999-05-01 00:00:00

  • Mutational analysis of Agrobacterium tumefaciens virD2: tyrosine 29 is essential for endonuclease activity.

    abstract::Agrobacterium tumefaciens VirD2 polypeptide, in the presence of VirD1, catalyzes a site- and strand-specific nicking reaction at the T-DNA border sequences. VirD2 is found tightly attached to the 5' end of the nicked DNA. The protein-DNA complex is presumably formed via a tyrosine residue of VirD2 (F. Durrenberger, A....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.1.303-308.1992

    authors: Vogel AM,Das A

    更新日期:1992-01-01 00:00:00

  • Target analysis of mitochondrial genetic units in yeast.

    abstract::Target analyses of the induction of rho(-) mutants indicated that the cytoplasm of an aerobic nonrepressed yeast contains approximately 20 mutable units, whereas repressed cells exhibit approximately 3. Aerobic adaptation of fully repressed cells brings about an increase in these cytoplasmic units which continues afte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.97.3.1142-1148.1969

    authors: Allen NE,MacQuillan AM

    更新日期:1969-03-01 00:00:00

  • Structural characterization and corepressor binding of the Escherichia coli purine repressor.

    abstract::The Escherichia coli purine repressor, PurR, binds to a 16-bp operator sequence and coregulates the genes for de novo synthesis of purine and pyrimidine nucleotides, formation of a one-carbon unit for biosynthesis, and deamination of cytosine. We have characterized the purified repressor. Chemical cross-linking indica...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.19.6207-6214.1992

    authors: Choi KY,Zalkin H

    更新日期:1992-10-01 00:00:00

  • Effects of treatment with sodium dodecyl sulfate on the ultrastructure of Escherichia coli.

    abstract::An electron microscopy study has been made of the effects of dissolution of the plasma membrane of Escherichia coli with sodium dodecyl sulfate (SDS) on the organization of the nucleoplasm and the cytoplasm. The alterations observed in time course experiments were related to absorbance changes and to release of macrom...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.111.3.801-813.1972

    authors: Woldringh CL,van Iterson W

    更新日期:1972-09-01 00:00:00

  • Coupling of asymmetric division to polar placement of replication origin regions in Bacillus subtilis.

    abstract::Entry into sporulation in Bacillus subtilis is characterized by the formation of a polar septum, which asymmetrically divides the developing cell into forespore (the smaller cell) and mother cell compartments, and by migration of replication origin regions to extreme opposite poles of the cell. Here we show that polar...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.13.4052-4060.2001

    authors: Graumann PL,Losick R

    更新日期:2001-07-01 00:00:00

  • Draft genome sequence of the quality control strain Enterococcus faecalis ATCC 29212.

    abstract::Enterococcus faecalis ATCC 29212, a vancomycin-sensitive strain, has been extensively used as a representative control strain for clinical and laboratory experiments. Here we report the draft genome and annotation of this strain, containing 3,027,060 bp, with a G+C content of 37.2% in 126 contigs (≥ 500 bp). ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01423-12

    authors: Kim EB,Kopit LM,Harris LJ,Marco ML

    更新日期:2012-11-01 00:00:00

  • Genome sequence of strain IMCC9480, a xanthorhodopsin-bearing betaproteobacterium isolated from the Arctic Ocean.

    abstract::Strain IMCC9480 is a novel member of the family Oxalobacteraceae of the Betaproteobacteria, isolated from the Arctic Ocean by dilution-to-extinction culturing. Here we present the draft genome sequence of strain IMCC9480. The genome is predicted to contain genes for xanthorhodopsin, retinoid biosynthesis, carbon monox...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05088-11

    authors: Oh HM,Lee K,Jang Y,Kang I,Kim HJ,Kang TW,Kim SY,Cho JC

    更新日期:2011-07-01 00:00:00

  • Isolation and characterization of a selenium metabolism mutant of Salmonella typhimurium.

    abstract::Selenium is a constituent in Escherichia coli of the anaerobic enzyme formate dehydrogenase in the form of selenocysteine. Selenium is also present in the tRNA of E. coli in the modified base 5-methylaminomethyl-2-selenouracil (mnm5Se2U). The pathways of bacterial selenium metabolism are largely uncharacterized, and i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.2.736-743.1988

    authors: Kramer GF,Ames BN

    更新日期:1988-02-01 00:00:00

  • Formation in the dark, of virus-induced deoxyribonuclease activity in Anacystis nidulans, an obligate photoautotroph.

    abstract::In Anacystis nidulans, upon infection with cyanophage AS-1, after a lag period of 1 h the level of deoxyribonuclease (DNase) activity increaded rapidly up to 15- to 20-fold in 4 to 5 h in the light. In contrast, the ribonuclease and phosphomonoesterase activities increased significantly only 4 to 5 h after infection, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.126.2.630-633.1976

    authors: Udvardy J,Sivok B,Borbely G,Farkas GL

    更新日期:1976-05-01 00:00:00

  • Envelope-associated folded chromosomes for Escherichia coli: variations under different physiological conditions.

    abstract::The folded chromosome of Escherichia coli has been investigated under various lysis and physiological conditions. A new gradient system was devised that allows excellent separation between unlysed cells and envelope-associated and envelope-free chromosomes. Isotope incorporation experiments showed that the fraction of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.2.904-916.1976

    authors: Korch C,Ovrebo S,Kleppe K

    更新日期:1976-08-01 00:00:00

  • D-glucaric acid and galactaric acid catabolism by Agrobacterium tumefaciens.

    abstract::Cell-free extract (crude extract) of Agrobacterium tumefaciens grown on d-glucuronate or d-glucarate converts d-glucarate and galactarate to a mixture of 2-keto-3-deoxy- and 4-deoxy-5-keto-d-glucarate. These compounds are then converted by partially purified crude extract to an intermediate tentatively identified as 2...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.102.1.85-96.1970

    authors: Chang YF,Feingold DS

    更新日期:1970-04-01 00:00:00

  • Cloning, sequencing, and expression in Escherichia coli of the gene encoding a 45-kilodalton protein, elongation factor Tu, from Chlamydia trachomatis serovar F.

    abstract::The gene encoding a 45-kDa protein (45K) of Chlamydia trachomatis serovar F was cloned, sequenced, and overexpressed in Escherichia coli. Alignment of the deduced peptide sequence with E. coli elongation factor Tu (EF-Tu) demonstrated 69% identity. The 45K was recognized by a Chlamydia genus-specific monoclonal antibo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.4.1184-1187.1994

    authors: Zhang YX,Shi Y,Zhou M,Petsko GA

    更新日期:1994-02-01 00:00:00

  • Potassium permeability of Rickettsia prowazekii.

    abstract::The potassium permeability of Rickettsia prowazekii was characterized by chemical measurement of the intracellular sodium and potassium pools and isotopic flux measurements with 86Rb+ as a tracer. R. prowazekii, in contrast to Escherichia coli, did not maintain a high potassium-to-sodium ratio in their cytoplasm excep...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.157.1.197-201.1984

    authors: Winkler HH

    更新日期:1984-01-01 00:00:00

  • Sigma factor RpoS controls alkylresorcinol synthesis through ArpR, a LysR-type regulatory protein, during encystment of Azotobacter vinelandii.

    abstract::Azotobacter vinelandii is a bacterium which undergoes a differentiation process leading to the formation of metabolically dormant cysts. During the encystment process, A. vinelandii produces alkylresorcinol lipids (ARs) that replace the membrane phospholipids and are also components of the layers covering the cyst. Th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01946-12

    authors: Romero Y,Moreno S,Guzmán J,Espín G,Segura D

    更新日期:2013-04-01 00:00:00

  • Gene disruption through homologous recombination in Spiroplasma citri: an scm1-disrupted motility mutant is pathogenic.

    abstract::To determine whether homologous recombination could be used to inactivate selected genes in Spiroplasma citri, plasmid constructs were designed to disrupt the motility gene scm1. An internal scm1 gene fragment was inserted into plasmid pKT1, which replicates in Escherichia coli but not in S. citri, and into the S. cit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.24.7449-7456.1999

    authors: Duret S,Danet JL,Garnier M,Renaudin J

    更新日期:1999-12-01 00:00:00

  • Biochemical and genetic investigation of initial reactions in aerobic degradation of the bile acid cholate in Pseudomonas sp. strain Chol1.

    abstract::Bile acids are surface-active steroid compounds with toxic effects for bacteria. Recently, the isolation and characterization of a bacterium, Pseudomonas sp. strain Chol1, growing with bile acids as the carbon and energy source was reported. In this study, initial reactions of the aerobic degradation pathway for the b...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00665-07

    authors: Birkenmaier A,Holert J,Erdbrink H,Moeller HM,Friemel A,Schoenenberger R,Suter MJ,Klebensberger J,Philipp B

    更新日期:2007-10-01 00:00:00

  • Flexibility of Vibrio cholerae ToxT in transcription activation of genes having altered promoter spacing.

    abstract::Cholera, a severe diarrheal disease, is caused by ingestion of the gram-negative bacterium Vibrio cholerae. Expression of V. cholerae virulence factors is highly regulated at the transcriptional and posttranscriptional levels by a complex network of proteins and small noncoding RNAs. The direct activator of transcript...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00512-08

    authors: Bellair M,Withey JH

    更新日期:2008-12-01 00:00:00

  • Proteus mirabilis mutants defective in swarmer cell differentiation and multicellular behavior.

    abstract::Proteus mirabilis is a dimorphic bacterium which exists in liquid cultures as a 1.5- to 2.0-microns motile swimmer cell possessing 6 to 10 peritrichous flagella. When swimmer cells are placed on a surface, they differentiate by a combination of events that ultimately produce a swarmer cell. Unlike the swimmer cell, th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.19.6279-6288.1991

    authors: Belas R,Erskine D,Flaherty D

    更新日期:1991-10-01 00:00:00

  • Formation of the K30 (group I) capsule in Escherichia coli O9:K30 does not require attachment to lipopolysaccharide lipid A-core.

    abstract::Escherichia coli K antigens (capsular polysaccharides) are divided into two broad classes, designated groups I and II, on the basis of a number of chemical, physical, and genetic criteria. Group I K antigens can be further subdivided on the basis of the absence (group IA) or presence (group IB) of amino sugars in the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.23.7515-7522.1993

    authors: MacLachlan PR,Keenleyside WJ,Dodgson C,Whitfield C

    更新日期:1993-12-01 00:00:00

  • ssaD1, a suppressor of secA51(Ts) that renders growth of Escherichia coli cold sensitive, is an early amber mutation in the transcription factor gene nusB.

    abstract::Complementation analysis of the ssaD1 mutation, isolated as a suppressor of the secA51(Ts) mutation that renders growth of Escherichia coli cold sensitive, was used to show that ssaD corresponds to nusB, a gene known to be important in transcription antitermination. DNA sequence analysis of the ssaD1 allele showed tha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.14.4444-4447.1994

    authors: Rajapandi T,Oliver D

    更新日期:1994-07-01 00:00:00

  • Regulation of the Salmonella typhimurium aroF gene in Escherichia coli.

    abstract::The Salmonella typhimurium aroF gene, encoding the tyrosine-sensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase, was localized to a chromosomal PstI fragment by Southern blotting with an Escherichia coli aroF probe. This fragment was cloned by screening a plasmid library for complementation of an E. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.5.2259-2266.1990

    authors: Muday GK,Herrmann KM

    更新日期:1990-05-01 00:00:00

  • Purification, properties, and regulation of glutamic dehydrogenase of Bacillus licheniformis.

    abstract::Cell-free extracts of Bacillus licheniformis and B. cereus were found to contain high specific activities of nicotinamide adenine dinucleotide phosphate (NADP)-dependent-l-glutamate dehydrogenase [EC 1.4.1.4; l-glutamate: NADP oxidoreductase (deaminating)]. Maximum specific activities were found in extracts of cells d...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.106.2.375-385.1971

    authors: Phibbs PV Jr,Bernlohr RW

    更新日期:1971-05-01 00:00:00

  • Processing and termination of 23S rRNA-5S rRNA-tRNA(Gly) primary transcripts in Thermus thermophilus HB8.

    abstract::The two 23S rRNA-5S rRNA-tRNAGly operons from the extreme thermophilic eubacterium Thermus thermophilus HB8 were used to characterized the in vivo processing and termination of 23S rRNA-5S rRNA-tRNAGly primary transcripts in this organism by nuclease S1 mapping. A processing site in the pre-23S rRNA 3'-flanking region...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.8.2681-2690.1991

    authors: Hartmann RK,Toschka HY,Erdmann VA

    更新日期:1991-04-01 00:00:00

  • Influence of iron on yields of extracellular products in Pseudomonas aeruginosa cultures.

    abstract::The effect of the iron content of the medium on the yields of extracellular products by seven distinct strains of Pseudomonas aeruginosa was examined. All strains showed at least an 85% decrease in toxin A yields when grown in medium containing 5.0 mug of iron per ml (high iron) as compared to 0.05 mug/ml (low iron), ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.138.1.193-200.1979

    authors: Bjorn MJ,Sokol PA,Iglewski BH

    更新日期:1979-04-01 00:00:00

  • Different roles for KinA, KinB, and KinC in the initiation of sporulation in Bacillus subtilis.

    abstract::Activation (phosphorylation) of the transcription factor encoded by spo0A is essential for the initiation of sporulation in Bacillus subtilis. At least three histidine protein kinases are involved in the phosphorylation of Spo0A. Under some growth conditions, KinA was the primary kinase, but under other conditions, Ki...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.3.861-863.1995

    authors: LeDeaux JR,Yu N,Grossman AD

    更新日期:1995-02-01 00:00:00

  • Defining the region of Bacillus subtilis SpoIIIJ that is essential for its sporulation-specific function.

    abstract::Proteins of the YidC/OxaI/Alb3 family play a crucial role in the insertion, folding, and/or assembly of membrane proteins in prokaryotes and eukaryotes. Bacillus subtilis has two YidC-like proteins, denoted SpoIIIJ and YqjG. SpoIIIJ and YqjG are largely exchangeable in function, but SpoIIIJ has a unique role in sporul...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01084-13

    authors: Geng Y,de Keyzer J,Scheffers DJ,Driessen AJ

    更新日期:2014-04-01 00:00:00

  • Metronidazole activation and isolation of Clostridium acetobutylicum electron transport genes.

    abstract::An Escherichia coli F19 recA, nitrate reductase-deficient mutant was constructed by transposon mutagenesis and shown to be resistant to metronidazole. This mutant was a most suitable host for the isolation of Clostridium acetobutylicum genes on recombinant plasmids, which activated metronidazole and rendered the E. co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.3.1088-1095.1991

    authors: Santangelo JD,Jones DT,Woods DR

    更新日期:1991-02-01 00:00:00

  • Characteristics of cold-sensitive mutants of Escherichia coli K-12 defective in deoxyribonucleic acid replication.

    abstract::Four cold-sensitive mutants of Escherichia coli have been isolated which show a reduced ability to synthesize deoxyribonucleic acid at low temperature. The mutants also have a reduced ability to incorporate nucleoside triphosphates into deoxyribonucleic acid at low temperature in cell preparations made permeable with ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.121.1.99-107.1975

    authors: Wehr CT,Waskell L,Glaser DA

    更新日期:1975-01-01 00:00:00

  • Interaction of protoplasts, L forms, and bacilli of Bacillus subtilis with 12 strains of bacteriophage.

    abstract::The interaction of 12 phage strains with bacilli, protoplasts, and L forms of Bacillus subtilis 168 and with eight of its mutants and two of its lysogens is described qualitatively and quantitatively. After removal of the cell wall from B. subtilis 168, 11 of the 12 phage strains can still adsorb to the protoplasts, n...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.124.1.445-448.1975

    authors: Jacobson ED,Landman OE

    更新日期:1975-10-01 00:00:00