An epitope of elongation factor Tu is widely distributed within the bacterial and archaeal domains.

Abstract:

:A monoclonal antibody (MAb), MAb 900, which detects a 43-kDa protein present on Escherichia coli was found. Subsequently, more than 90 organisms, belonging to either the bacterial, archaeal, or eucaryal domain, were tested for reactivity to this MAb. Of the bacterial and archaeal domains, almost all species proved to be positive, whereas all organisms from the eucaryal domain gave negative results. The 43-kDa protein was purified by affinity chromatography and subsequently analyzed by microsequencing methods. Two peptide sequences which showed a high degree of homology (> 99%) to the prokaryotic elongation factor Tu (EF-Tu) were obtained. Western blot (immunoblot) analysis using both purified EF-Tu and EF-Tu domains confirmed that the unknown protein was EF-Tu. The panbacterial distribution of EF-Tu, which is present in large amounts in every prokaryotic cell, renders this protein a good candidate for a diagnostic approach. In consequence, we have used the anti-EF-Tu MAb 900 to design both a dot blot assay and an enzyme-linked immunosorbent assay. From either blood culture, urine, or gall-bladder fluid, bacterial contamination could be detected. The sensitivity of these tests is currently 10(4) bacteria per ml.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Weber S,Lottspeich F,Köhl J

doi

10.1128/jb.177.1.11-19.1995

subject

Has Abstract

pub_date

1995-01-01 00:00:00

pages

11-9

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

177

pub_type

杂志文章
  • Salmonella FraE, an Asparaginase Homolog, Contributes to Fructose-Asparagine but Not Asparagine Utilization.

    abstract::Salmonella enterica can utilize fructose-asparagine (F-Asn) as a source of carbon and nitrogen. This capability has been attributed to five genes in the fra locus. Previously, we determined that mutations in fraB (deglycase), fraD (kinase), or fraA (transporter) eliminated the ability of Salmonella to grow on F-Asn, w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00330-17

    authors: Sabag-Daigle A,Sengupta A,Blunk HM,Biswas PK,Cron MC,Bogard AJ,Behrman EJ,Gopalan V,Ahmer BMM

    更新日期:2017-10-17 00:00:00

  • Nucleotide sequence of the McrB region of Escherichia coli K-12 and evidence for two independent translational initiation sites at the mcrB locus.

    abstract::The McrB restriction system of Escherichia coli K-12 is responsible for the biological inactivation of foreign DNA that contains 5-methylcytosine residues (E. A. Raleigh and G. Wilson, Proc. Natl. Acad. Sci. USA 83:9070-9074, 1986). Within the McrB region of the chromosome is the mcrB gene, which encodes a protein of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.4.1974-1981.1989

    authors: Ross TK,Achberger EC,Braymer HD

    更新日期:1989-04-01 00:00:00

  • Genome sequence of the Corynebacterium pseudotuberculosis Cp316 strain, isolated from the abscess of a Californian horse.

    abstract::The bacterium Corynebacterium pseudotuberculosis is of major veterinary importance because it affects livestock, particularly sheep, goats, and horses, in several countries, including Australia, Brazil, the United States, and Canada, resulting in significant economic losses. In the present study, we describe the compl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01616-12

    authors: Ramos RT,Silva A,Carneiro AR,Pinto AC,Soares Sde C,Santos AR,Almeida SS,Guimarães LC,Aburjaile FF,Barbosa EG,Dorella FA,Rocha FS,Cerdeira LT,Barbosa MS,Tauch A,Edman J,Spier S,Miyoshi A,Schneider MP,Azevedo V

    更新日期:2012-12-01 00:00:00

  • Molecular recombination between R-factor deoxyribonucleic acid molecules in Escherichia coli host cells.

    abstract::THREE PREVIOUSLY STUDIED R FACTORS WERE USED: 222/R4, controlling transmissible resistance to sulfonamide, streptomycin, chloromycetin, and tetracycline (SU(r) SM(r) CM(r) TC(r)); 222/R3, a derivative of 222/R4 (now termed 222/R3W) having lost TC(r); and R15, controlling infectious resistance to SU and SM only. Two ty...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.103.1.166-177.1970

    authors: Nisioka T,Mitani M,Clowes RC

    更新日期:1970-07-01 00:00:00

  • IS1549 from Mycobacterium smegmatis forms long direct repeats upon insertion.

    abstract::A new insertion element, IS1549, was identified serendipitously from Mycobacterium smegmatis LR222 during experiments using a vector designed to detect the excision of IS6110 from between the promoter region and open reading frame (ORF) of an aminoglycoside phosphotransferase gene. Six of the kanamycin-resistant isola...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.5.1037-1043.1998

    authors: Plikaytis BB,Crawford JT,Shinnick TM

    更新日期:1998-03-01 00:00:00

  • Strain-Dependent RstA Regulation of Clostridioides difficile Toxin Production and Sporulation.

    abstract::The anaerobic spore former Clostridioides difficile causes significant diarrheal disease in humans and other mammals. Infection begins with the ingestion of dormant spores, which subsequently germinate within the host gastrointestinal tract. There, the vegetative cells proliferate and secrete two exotoxins, TcdA and T...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00586-19

    authors: Edwards AN,Krall EG,McBride SM

    更新日期:2020-01-02 00:00:00

  • Overproduction of SecA suppresses the export defect caused by a mutation in the gene encoding the Escherichia coli export chaperone secB.

    abstract::SecB is a cytosolic protein required for rapid and efficient export of particular periplasmic and outer membrane proteins in Escherichia coli. SecB promotes export by stabilizing newly synthesized precursor proteins in a nonnative conformation and by targeting the precursors to the inner membrane. Biochemical studies ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.10.3010-3017.1999

    authors: Cook HA,Kumamoto CA

    更新日期:1999-05-01 00:00:00

  • Single-stranded DNA binding by F TraI relaxase and helicase domains is coordinately regulated.

    abstract::Transfer of conjugative plasmids requires relaxases, proteins that cleave one plasmid strand sequence specifically. The F plasmid relaxase TraI (1,756 amino acids) is also a highly processive DNA helicase. The TraI relaxase activity is located within the N-terminal approximately 300 amino acids, while helicase motifs ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00154-10

    authors: Dostál L,Schildbach JF

    更新日期:2010-07-01 00:00:00

  • High-frequency mobilization of broad-host-range plasmids into Neisseria gonorrhoeae requires methylation in the donor.

    abstract::Antibiotic resistance in Neisseria gonorrhoeae has been associated with the acquisition of R plasmids from heterologous organisms. The broad-host-range plasmids of incompatibility groups P (IncP) and Q (IncQ) have played a role in this genetic exchange in nature. We have utilized derivatives of RSF1010 (IncQ) and RP1 ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.18.5793-5799.1991

    authors: Butler CA,Gotschlich EC

    更新日期:1991-09-01 00:00:00

  • Sequence analysis of the hutH gene encoding histidine ammonia-lyase in Pseudomonas putida.

    abstract::The complete nucleotide sequence of the hutH gene, encoding histidine ammonia-lyase (histidase), in Pseudomonas putida ATCC 12633 has been determined from the appropriate portions of the hut region that had been cloned into Escherichia coli. The resulting DNA sequence revealed an open reading frame of 1,530 base pairs...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.5.2224-2229.1990

    authors: Consevage MW,Phillips AT

    更新日期:1990-05-01 00:00:00

  • dacD, an Escherichia coli gene encoding a novel penicillin-binding protein (PBP6b) with DD-carboxypeptidase activity.

    abstract::In the course of a study of genes located at min 44 of the Escherichia coli genome, we identified an open reading frame with the capacity to encode a 43-kDa polypeptide whose predicted amino acid sequence is strikingly similar to those of the well-known DD-carboxipeptidases penicillin-binding proteins PBP5 and PBP6. T...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.24.7106-7111.1996

    authors: Baquero MR,Bouzon M,Quintela JC,Ayala JA,Moreno F

    更新日期:1996-12-01 00:00:00

  • ISOLOGOUS INTERFERENCE WITH ULTRAVIOLET AND X-RAY IRRADIATED BACTERIOPHAGE T2.

    abstract::Levy, Stuart B. (Institut du Radium, Paris, France). Isologous interference with ultraviolet and X-ray irradiated bacteriophage T(2). J. Bacteriol. 87:1330-1338. 1964.-Qualitative and quantitative analysis of the interference capacity of an irradiated T(2) bacteriophage was made with ultraviolet and X-ray irradiation....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.6.1330-1338.1964

    authors: LEVY SB

    更新日期:1964-06-01 00:00:00

  • Gene involved in transcriptional activation of the hrp regulatory gene hrpG in Xanthomonas oryzae pv. oryzae.

    abstract::A novel regulatory gene, trh, which is involved in hrp gene expression, is identified in the plant pathogen Xanthomonas oryzae pv. oryzae. In the trh mutant, expression of HrpG, which is a key regulator for hrp gene expression, is reduced both under the in vitro hrp-inducing condition and in planta. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00006-06

    authors: Tsuge S,Nakayama T,Terashima S,Ochiai H,Furutani A,Oku T,Tsuno K,Kubo Y,Kaku H

    更新日期:2006-06-01 00:00:00

  • Replication of the nonconjugative plasmid RSF1010 in Escherichia coli K-12.

    abstract::Replicating DNA molecules of the nonconjugative R plasmid RSF1010 (Smr Sur) were cleaved with the EcoRI restriction endonuclease and examined with the electron microscope. Results of this analysis indicated that replication is initiated from an origin located at about 19% of total genome size from one of the EcoRI end...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.134.3.1117-1122.1978

    authors: de Graaff J,Crosa JH,Heffron F,Falkow S

    更新日期:1978-06-01 00:00:00

  • A collagen-binding adhesin, Acb, and ten other putative MSCRAMM and pilus family proteins of Streptococcus gallolyticus subsp. gallolyticus (Streptococcus bovis Group, biotype I).

    abstract::Members of the Streptococcus bovis group are important causes of endocarditis. However, factors associated with their pathogenicity, such as adhesins, remain uncharacterized. We recently demonstrated that endocarditis-derived Streptococcus gallolyticus subsp. gallolyticus isolates frequently adhere to extracellular ma...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00909-09

    authors: Sillanpää J,Nallapareddy SR,Qin X,Singh KV,Muzny DM,Kovar CL,Nazareth LV,Gibbs RA,Ferraro MJ,Steckelberg JM,Weinstock GM,Murray BE

    更新日期:2009-11-01 00:00:00

  • Isolation of flagella from the archaebacterium Methanococcus voltae by phase separation with Triton X-114.

    abstract::The flagella of Methanococcus voltae were isolated by using three procedures. Initially, cells were sheared to release the filaments, which were purified by differential centrifugation and banding in KBr gradients. Flagella were also prepared by solubilization of cells with 1% (vol/vol) Triton X-100 and purified as de...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.4.1752-1758.1988

    authors: Kalmokoff ML,Jarrell KF,Koval SF

    更新日期:1988-04-01 00:00:00

  • Impaired expression of certain prereplicative bacteriophage T4 genes explains impaired T4 DNA synthesis in Escherichia coli rho (nusD) mutants.

    abstract::The Escherichia coli rho 026 mutation that alters the transcription termination protein Rho prevents growth of wild-type bacteriophage T4. Among the consequences of this mutation are delayed and reduced T4 DNA replication. We show that these defects can be explained by defective synthesis of certain T4 replication-rec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.7.3872-3880.1989

    authors: Stitt BL,Mosig G

    更新日期:1989-07-01 00:00:00

  • Serology of Listeria monocytogenes. I. Characteristics of the soluble hemolysin.

    abstract::Girard, Kenneth F. (St. Margaret's Hospital, Boston, Mass.), Anthony J. Sbarra, and Wadi A. Bardawil. Serology of Listeria monocytogenes. I. Characteristics of the soluble hemolysin. J. Bacteriol. 85:349-355. 1963.-Our results clearly demonstrate that Listeria monocytogenes (strain 9-125) produces a soluble hemolysin....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.85.2.349-355.1963

    authors: GIRARD KF,SBARRA AJ,BARDAWIL WA

    更新日期:1963-02-01 00:00:00

  • Nucleotide sequences of the fecBCDE genes and locations of the proteins suggest a periplasmic-binding-protein-dependent transport mechanism for iron(III) dicitrate in Escherichia coli.

    abstract::The fec region of the Escherichia coli chromosome determines a citrate-dependent iron(III) transport system. The nucleotide sequence of fec revealed five genes, fecABCDE, which are transcribed from fecA to fecE. The fecA gene encodes a previously described outer membrane receptor protein. The fecB gene product is form...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.5.2626-2633.1989

    authors: Staudenmaier H,Van Hove B,Yaraghi Z,Braun V

    更新日期:1989-05-01 00:00:00

  • Effects of cortex peptidoglycan structure and cortex hydrolysis on the kinetics of Ca(2+)-dipicolinic acid release during Bacillus subtilis spore germination.

    abstract::The kinetic parameters of the release of Ca(2+)-dipicolinic acid (CaDPA) during germination of spore populations and multiple individual spores of Bacillus subtilis strains with major alterations in the structure of the spore peptidoglycan (PG) cortex or lacking one or both of the two redundant enzymes involved in cor...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06452-11

    authors: Zhang P,Thomas S,Li YQ,Setlow P

    更新日期:2012-02-01 00:00:00

  • Chloramphenicol-sensitive and-insensitive phases of the lethal action of streptomycin.

    abstract::Hurwitz, Charles (Veterans Administration Hospital, Albany, N.Y.) and Carmen L. Rosano. Chloramphenicol-sensitive and -insensitive phases of the lethal action of streptomycin. J. Bacteriol. 83:1202-1209. 1962.-If chloramphenicol is added to sensitive Escherichia coli cells at the same time as streptomycin, the lethal ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.6.1202-1209.1962

    authors: HURWITZ C,ROSANO CL

    更新日期:1962-06-01 00:00:00

  • The integrase of the conjugative transposon Tn916 directs strand- and sequence-specific cleavage of the origin of conjugal transfer, oriT, by the endonuclease Orf20.

    abstract::Orf20 of the conjugative transposon Tn916 was purified as a chimeric protein fused to maltose binding protein (MBP-Orf20). The chimeric protein possessed endonucleolytic activity, cleaving both strands of the Tn916 origin of conjugal transfer (oriT) at several distinct sites and favoring GT dinucleotides. Incubation o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.6.2207-2213.2006

    authors: Rocco JM,Churchward G

    更新日期:2006-03-01 00:00:00

  • Two amino acid residues of transposase contributing to differential transposability of IS1 elements in Escherichia coli.

    abstract::Escherichia coli W3110 contains four types of IS1 elements in the chromosome. Using an insertion element entrapping system, we collected 116 IS1 plasmid insertion mutants, which resulted from a minimum of 26 independent IS1 insertion events. All of them had insertions of IS1 of the IS1A (IS1E and IS1G) type. Inspectio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.19.5279-5283.1998

    authors: Chen JH,Hsu WB,Hwang JL

    更新日期:1998-10-01 00:00:00

  • Regulation of bacteriocin production in Streptococcus mutans by the quorum-sensing system required for development of genetic competence.

    abstract::In Streptococcus mutans, competence for genetic transformation and biofilm formation are dependent on the two-component signal transduction system ComDE together with the inducer peptide pheromone competence-stimulating peptide (CSP) (encoded by comC). Here, it is shown that the same system is also required for expres...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.12.3980-3989.2005

    authors: van der Ploeg JR

    更新日期:2005-06-01 00:00:00

  • Isocitrate dehydrogenase kinase/phosphatase: aceK alleles that express kinase but not phosphatase activity.

    abstract::For Escherichia coli, growth on acetate requires the induction of the enzymes of the glyoxylate bypass, isocitrate lyase and malate synthase. The branch point between the glyoxylate bypass and the Krebs cycle is controlled by phosphorylation of isocitrate dehydrogenase (IDH), inhibiting that enzyme's activity and thus...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.5.1801-1806.1991

    authors: Ikeda T,LaPorte DC

    更新日期:1991-03-01 00:00:00

  • Inhibitory effect of novobiocin on ribonucleic acid synthesis during germination of Bacillus subtilis spores.

    abstract::Novobiocin inhibited ribonculeic acid synthesis during germination of Bacillus subtilis spores. Transcription of certain kinds of genes probably required a preceding conformational change in deoxyribonucleic acid. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.142.1.366-368.1980

    authors: Matsuda M,Kameyama T

    更新日期:1980-04-01 00:00:00

  • Conserved cytoplasmic loops are important for both the transport and chemotaxis functions of PcaK, a protein from Pseudomonas putida with 12 membrane-spanning regions.

    abstract::Chemotaxis to the aromatic acid 4-hydroxybenzoate (4-HBA) by Pseudomonas putida is mediated by PcaK, a membrane-bound protein that also functions as a 4-HBA transporter. PcaK belongs to the major facilitator superfamily (MFS) of transport proteins, none of which have so far been implicated in chemotaxis. Work with two...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.16.5068-5074.1999

    authors: Ditty JL,Harwood CS

    更新日期:1999-08-01 00:00:00

  • Evidence from Mutational Analysis for a Single Transmembrane Substrate Binding Site in the Histidine ATP-Binding Cassette Transporter of Salmonella enterica Serovar Typhimurium.

    abstract::The histidine ATP-binding cassette (ABC) transporter of Salmonella enterica serovar Typhimurium is among the best-studied type I ABC import systems. The transporter consists of two transmembrane subunits, HisQ and HisM, and a homodimer of the nucleotide-binding subunit, HisP. Substrates are delivered by two periplasmi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00521-18

    authors: Heuveling J,Landmesser H,Schneider E

    更新日期:2018-12-20 00:00:00

  • Multiple forms of lysyl-transfer ribonucleic acid synthetase in Escherichia coli.

    abstract::Lysyl-transfer ribonucleic acid synthetase (EC 6.1.1.6) was identified as four polypeptide spots after two-dimensional polyacrylamide gel electrophoresis of whole-cell lysates of Escherichia coli. Identification was made by migration with partially purified enzyme preparations, by peptide map patterns, by mutant analy...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.146.1.345-351.1981

    authors: Hirshfield IN,Bloch PL,Van Bogelen RA,Neidhardt FC

    更新日期:1981-04-01 00:00:00

  • Spontaneous erythromycin resistance mutation in a 23S rRNA gene, rrlA, of the extreme thermophile Thermus thermophilus IB-21.

    abstract::Spontaneous, erythromycin-resistant mutants of Thermus thermophilus IB-21 were isolated and found to carry the mutation A2058G in one of two 23S rRNA operons. The heterozygosity of these mutants indicates that A2058G confers a dominant or codominant phenotype in this organism. This mutation provides a valuable tool fo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.14.4382-4385.2001

    authors: Gregory ST,Cate JH,Dahlberg AE

    更新日期:2001-07-01 00:00:00