Abstract:
:The complete nucleotide sequence of the hutH gene, encoding histidine ammonia-lyase (histidase), in Pseudomonas putida ATCC 12633 has been determined from the appropriate portions of the hut region that had been cloned into Escherichia coli. The resulting DNA sequence revealed an open reading frame of 1,530 base pairs, corresponding to a protein subunit of approximate molecular weight 53,600, in the location previously identified for the histidase gene by Tn1000 mutagenesis. Translation began at a GTG codon, but direct protein sequencing revealed that the initiating amino acid was removed posttranslationally to provide an N-terminal threonine; 11 additional residues completely agreed with the predicted amino acid sequence. This sequence excluded the possibility that a dehydroalanine unit, the postulated coenzyme for histidase, is attached at the N terminus of histidase subunits. Comparison of the P. putida histidase gene sequence with that of a Bacillus subtilis region encoding histidase revealed 42% identity at the protein level. Although the hutU (urocanase) and hutH (histidase) genes are induced by urocanate and normally are transcribed as a unit beginning with hutU, analysis of the region immediately upstream of the histidase gene revealed a potential weak promoter that may possibly be used to maintain a basal level of histidase for the generation of inducer (urocanate) when histidine levels are elevated.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Consevage MW,Phillips ATdoi
10.1128/jb.172.5.2224-2229.1990subject
Has Abstractpub_date
1990-05-01 00:00:00pages
2224-9issue
5eissn
0021-9193issn
1098-5530journal_volume
172pub_type
杂志文章abstract::The potent tumor-promoting agent croton oil, which has been shown previously to be strongly mitogenic in mammalian cells, stimulates cell division in snake mutants of the blue-green bacterium Agmenellum quadruplicatum. ...
journal_title:Journal of bacteriology
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doi:10.1128/JB.114.2.874-875.1973
更新日期:1973-05-01 00:00:00
abstract::Girard, Kenneth F. (St. Margaret's Hospital, Boston, Mass.), Anthony J. Sbarra, and Wadi A. Bardawil. Serology of Listeria monocytogenes. I. Characteristics of the soluble hemolysin. J. Bacteriol. 85:349-355. 1963.-Our results clearly demonstrate that Listeria monocytogenes (strain 9-125) produces a soluble hemolysin....
journal_title:Journal of bacteriology
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pub_type: 杂志文章
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更新日期:1992-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.140.2.567-573.1979
更新日期:1979-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.19.6298-6302.1992
更新日期:1992-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01047-07
更新日期:2007-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.115.3.746-751.1973
更新日期:1973-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.124.3.1148-1152.1975
更新日期:1975-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.7.3080-3088.1988
更新日期:1988-07-01 00:00:00
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
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更新日期:2008-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00697-13
更新日期:2013-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.2.793-801.1990
更新日期:1990-02-01 00:00:00
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更新日期:2007-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.8.3742-3746.1988
更新日期:1988-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1965-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1977-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1995-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1984-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.7.2406-2408.1991
更新日期:1991-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-09-01 00:00:00