Abstract:
:SecB is a cytosolic protein required for rapid and efficient export of particular periplasmic and outer membrane proteins in Escherichia coli. SecB promotes export by stabilizing newly synthesized precursor proteins in a nonnative conformation and by targeting the precursors to the inner membrane. Biochemical studies suggest that SecB facilitates precursor targeting by binding to the SecA protein, a component of the membrane-embedded translocation apparatus. To gain more insight into the functional interaction of SecB and SecA, in vivo, mutations in the secA locus that compensate for the export defect caused by the secB missense mutation secBL75Q were isolated. Two suppressors were isolated, both of which led to the overproduction of wild-type SecA protein. In vivo studies demonstrated that the SecBL75Q mutant protein releases precursor proteins at a lower rate than does wild-type SecB. Increasing the level of SecA protein in the cell was found to reverse this slow-release defect, indicating that overproduction of SecA stimulates the turnover of SecBL75Q-precursor complexes. These findings lend additional support to the proposed pathway for precursor targeting in which SecB promotes targeting to the translocation apparatus by binding to the SecA protein.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Cook HA,Kumamoto CAdoi
10.1128/JB.181.10.3010-3017.1999keywords:
subject
Has Abstractpub_date
1999-05-01 00:00:00pages
3010-7issue
10eissn
0021-9193issn
1098-5530journal_volume
181pub_type
杂志文章abstract::The gene that encodes the acyl carrier protein (ACP) of the actinorhodin polyketide synthase (PKS) of Streptomyces coelicolor A3(2) was replaced with homologs from the granaticin, oxytetracycline, tetracenomycin, and putative frenolicin polyketide synthase gene clusters. All of the replacements led to expression of fu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.8.2197-2204.1993
更新日期:1993-04-01 00:00:00
abstract::The ferric enterobactin (FeEnt) receptor CfrA is present in the majority of Campylobacter jejuni isolates and is responsible for high-affinity iron acquisition. Our recent work and that of others strongly suggested the existence of another FeEnt uptake system in Campylobacter. Here we have identified and characterized...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00478-10
更新日期:2010-09-01 00:00:00
abstract::Neonatal bacterial meningitis continues to be an important cause of mortality and morbidity worldwide. Escherichia coli possessing the K1 capsular polysaccharide is the most common Gram-negative pathogen causing neonatal meningitis. Here we present the complete genome sequence of neonatal meningitis-associated E. coli...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06284-11
更新日期:2011-12-01 00:00:00
abstract::An unusual xylose isomerase produced by Thermoanaerobacterium strain JW/SL-YS 489 was purified 28-fold to gel electrophoretic homogeneity, and the biochemical properties were determined. Its pH optimum distinguishes this enzyme from all other previously described xylose isomerases. The purified enzyme had maximal acti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.20.5938-5945.1996
更新日期:1996-10-01 00:00:00
abstract::Structural analogs of S-adenosylhomocysteine were tested in vitro for inhibition of the yeast S-adenosylmethionine:delta 24-sterol-C-methyltransferase enzyme. A wide inhibitory range by these compounds was observed, suggesting which structural features of the parent compound are important for binding to the enzyme. No...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.1.106-112.1981
更新日期:1981-01-01 00:00:00
abstract::Dienelactone hydrolases have previously been shown to play a crucial role in chlorocatechol degradation via the modified ortho cleavage pathway. Recently, the enzymes induced in 4-fluorobenzoate-utilizing bacteria have been classified into three groups on the basis of their specificity towards cis- and trans-dienelact...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.10.2994-3001.1993
更新日期:1993-05-01 00:00:00
abstract::The presence of acetate and pyruvate groups in Klebsiella capsular polysaccharides may be demonstrated and estimated quantitatively by running the proton magnetic resonance spectrum of the polysaccharide (as sodium salt) in deuterium oxide at 95 C. Such spectra also permit an assessment to be made of the number of alp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.3.1345-1347.1973
更新日期:1973-03-01 00:00:00
abstract::All bacteria use the conserved Sec pathway to transport proteins across the cytoplasmic membrane, with the SecA ATPase playing a central role in the process. Mycobacteria are part of a small group of bacteria that have two SecA proteins: the canonical SecA (SecA1) and a second, specialized SecA (SecA2). The SecA2-depe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00630-13
更新日期:2013-10-01 00:00:00
abstract::Bacillus anthracis Sterne cured of the pXO1 plasmid had enhanced secreted protease activity during the postexponential phase but no change in hemolytic or lecithinase activities. A zymogen profile revealed at least six proteases, including serine, metal, and perhaps cysteine types. There were similar amounts of protea...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.9.3133-3138.2005
更新日期:2005-05-01 00:00:00
abstract::Deletion mutagenesis analysis of a duplicated gene necessary for Haemophilus influenzae serotype b capsule expression showed that only one functional copy of this gene is required for capsule production and for virulence in infant rats. Mutant strains generated in this study differed from each other and from the paren...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.1.44-48.1986
更新日期:1986-07-01 00:00:00
abstract::Evidence obtained with an improved in vivo assay of fimbrial phase variation in Escherichia coli supported a revised understanding of the roles of fimB and fimE in the site-specific DNA rearrangement with which they are associated. A previously proposed model argued that fimB and fimE play antagonistic, unidirectional...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.17.5308-5314.1991
更新日期:1991-09-01 00:00:00
abstract::We isolated and characterized crp mutations in Escherichia coli that allow cyclic AMP (cAMP) receptor protein to function without cAMP. These mutants defined a region involved in the cAMP-induced allosteric change of cAMP receptor protein that is necessary for activation of the protein. Currently, we have isolated int...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.4.1417-1422.1988
更新日期:1988-04-01 00:00:00
abstract::Desulfosporosinus species are sulfate-reducing bacteria belonging to the Firmicutes. Their genomes will give insights into the genetic repertoire and evolution of sulfate reducers typically thriving in terrestrial environments and able to degrade toluene (Desulfosporosinus youngiae), to reduce Fe(III) (Desulfosporosin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01392-12
更新日期:2012-11-01 00:00:00
abstract::Streptomyces coelicolor produces four known antibiotics. To define genetic elements that regulate antibiotic synthesis, we screened for mutations that visibly blocked synthesis of the two pigmented antibiotics and found that the mutant strains which we recovered were of two classes--double mutants and mutants in which...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.6.2962-2969.1990
更新日期:1990-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.2.449-457.1968
更新日期:1968-02-01 00:00:00
abstract::A novel mutant of the marine oil-degrading bacterium Alcanivorax borkumensis SK2, containing a mini-Tn5 transposon disrupting a "tesB-like" acyl-coenzyme A (CoA) thioesterase gene, was found to hyperproduce polyhydroxyalkanoates (PHA), resulting in the extracellular deposition of this biotechnologically important poly...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01321-06
更新日期:2006-12-01 00:00:00
abstract::A Pediococcus cerevisiae mutant that actively accumulated folate (PteGlu), in contrast to the wild-type, was also found to exhibit changes in the pattern of uptake of 5-methyl-tetrahydrofolate (5-CH3-H4PteGlu) and amethopterin. Most of the 5-CH3-H4PteGlue accumulated through a glucose- and temperature-dependent proces...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.123.2.400-406.1975
更新日期:1975-08-01 00:00:00
abstract::Lundgren, D. G. (Syracuse University, Syracuse, N.Y.) and J. J. Cooney. Chemical analyses of asporogenic mutants of Bacillus cereus. J. Bacteriol. 83:1287-1293. 1962.-Bacillus cereus ATCC 4342 and three temperature-sensitive asporogenic mutants were compared regarding some basic cellular components and response to met...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.6.1287-1293.1962
更新日期:1962-06-01 00:00:00
abstract::Streptococcus faecalis transposon Tn917 was cloned in Escherichia coli on plasmid vector pBR325. The erythromycin resistance determinant of Tn917 was not expressed in the E. coli background. The nucleotide sequence of Tn917 was determined and found to be 5,257 base pairs in length. Six open reading frames (ORFs) were ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.2.782-796.1985
更新日期:1985-11-01 00:00:00
abstract::The halophilic bacterium Halomonas elongata synthesizes as its main compatible solute the aspartate derivative ectoine. We constructed a deletion mutant of H. elongata, KB1, defective in ectoine synthesis and tolerating elevated salt concentrations only in the presence of external compatible solutes. The dependency of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.11.3078-3085.2002
更新日期:2002-06-01 00:00:00
abstract::We report the complete and annotated genome sequence of the nonpathogenic Listeria seeligeri SLCC3954 serovar 1/2b type strain harboring the smallest completely sequenced genome of the genus Listeria. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01415-09
更新日期:2010-03-01 00:00:00
abstract::Two inhibitors of replicative deoxyribonucleic acid (DNA) synthesis, nalidixic acid (NAL) and 6-(p-hydroxyphenylazo)-uracil (HPUra), showed different effects on genetic recombination and DNA repair in Bacillus subtilis. Previous work (Pedrini et al., 1972) showed that NAL does not interfere with the transformation pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.126.1.108-121.1976
更新日期:1976-04-01 00:00:00
abstract::Plasmid pUB110 was previously used as a vector to clone fragments of deoxyribonucleic acid that complement the trpC2 mutation in Bacillus subtilis from endonuclease EcoRI digested B. licheniformis, B. pumilus, and B. subtilis cellular deoxyribonucleic acid. Each of several such trp plasmids was subsequently shown to c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.3.1001-1006.1979
更新日期:1979-09-01 00:00:00
abstract::Adenosine 5'-triphosphate (ATP) synthesis energized by an artificially imposed protonmotive force (delta p) in adenosine 5'-diphosphate-loaded membrane vesicles of Escherichia coli was investigated. The protonmotive force is composed of an artificially imposed pH gradient (delta pH) or membrane potential (deltapsi), o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.2.763-769.1977
更新日期:1977-02-01 00:00:00
abstract::Cefsulodin lyses actively growing Escherichia coli by binding specifically to penicillin-binding proteins (PBPs) 1a and 1b. Recent findings (F. García del Portillo, M. A. de Pedro, D. Joseleau-Petit, and R. D'Ari, J. Bacteriol. 171:4217-4221, 1989) have linked cefsulodin-induced lysis to septation during the first div...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.1.1-5.1991
更新日期:1991-01-01 00:00:00
abstract::An extremely thermophilic bacterium, Thermus thermophilus HB8, is one of the model organisms for systems biology. Its genome consists of a chromosome (1.85 Mb), a megaplasmid (0.26 Mb) designated pTT27, and a plasmid (9.3 kb) designated pTT8, and the complete sequence is available. We show here that T. thermophilus is...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00662-10
更新日期:2010-10-01 00:00:00
abstract::The enzyme pattern of Saccharomyces cerevisiae was followed during batch growth and in continuous culture in a synthetic medium limited for glucose under aerobic conditions. Seven enzymes were measured: succinate-cytochrome c oxidoreductase, malate dehydrogenase, nicotinamide adenine dinucleotide-linked glutamate dehy...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.2.479-486.1968
更新日期:1968-08-01 00:00:00
abstract::Rickettsia rickettsii, the causative agent of Rocky Mountain spotted fever, contains two immunodominant proteins, rOmpA and rOmpB, in the outer membrane. Both rOmpA and rOmpB are conserved throughout spotted fever group rickettsiae as members of a family of autotransporter proteins. Previously, it was demonstrated tha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00826-16
更新日期:2017-02-28 00:00:00
abstract::Fifteen low-temperature conditional division mutants of Escherichia coli K-12 was isolated. They grew normally at 39 degrees C but formed filaments at 30 degrees C. All exhibited a coordinated burst of cell division when the filaments were shifted to the permissive temperature (39 degrees C). None of the various agent...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.1.256-264.1978
更新日期:1978-01-01 00:00:00
abstract::The amounts of glycogen and trehalose have been measured in cells of a prototrophic diploid yeast strain subjected to a variety of nutrient limitations. Both glycogen and trehalose were accumulated in cells deprived specifically of nirogen, sulfur, or phosphorus, suggesting that reserve carbohydrate accumulation is a ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.3.1384-1394.1980
更新日期:1980-09-01 00:00:00