Two amino acid residues of transposase contributing to differential transposability of IS1 elements in Escherichia coli.

abstract：:The energetic efficiency of microbial growth is significantly reduced in cultures growing under glucose excess compared to cultures growing under glucose limitation, but the magnitude to which different energy-dissipating processes contribute to the reduced efficiency is currently not well understood. We introduce her...

journal_title：Journal of bacteriology

authors： Dauner M,Storni T,Sauer U

更新日期：2001-12-01 00:00:00

abstract：:We previously described mutations in a gene, secB, which have pleiotropic effects on protein export in Escherichia coli. In this paper, we report the isolation of mutants in which the activity of the secB gene was eliminated. Null mutations in secB affected only a subset of exported proteins. Strains carrying these mu...

journal_title：Journal of bacteriology

authors： Kumamoto CA,Beckwith J

更新日期：1985-07-01 00:00:00

abstract：:Twenty-six FliF monomers assemble into the MS ring, a central motor component of the bacterial flagellum that anchors the structure in the inner membrane. Approximately 100 amino acids at the C terminus of FliF are exposed to the cytoplasm and, through the interaction with the FliG switch protein, a component of the f...

journal_title：Journal of bacteriology

authors： Grünenfelder B,Gehrig S,Jenal U

更新日期：2003-03-01 00:00:00

abstract：:Expression of fimA, the structural gene for type 1 fimbriae of Escherichia coli, is phase variable. Significant homologies were identified between the recombinases which control fimbrial phase variation, FimB and FimE, and the integrase class of site-specific recombinases. Normal expression of fimA was shown to requir...

journal_title：Journal of bacteriology

authors： Dorman CJ,Higgins CF

更新日期：1987-08-01 00:00:00

abstract：:Escherichia coli UM146 was isolated from the ileum of a Crohn's disease patient. It adheres to and invades enterocytes and can replicate inside macrophages. Its complete genome sequence reveals that it is most closely related to the human urinary tract pathogen E. coli CFT073, but it has a host of genes that are novel...

journal_title：Journal of bacteriology

authors： Krause DO,Little AC,Dowd SE,Bernstein CN

更新日期：2011-01-01 00:00:00

abstract：:Genetic analysis of a d-alanine requiring mutant (dal) of Bacillus subtilis reveals that the gene that codes for d,l-alanine racemase is linked to purB. The order of genes in this region of the chromosome is purB, pig, tsi, dal. Thus there are at least two clusters of genes that regulate cell wall biosynthesis in B. s...

journal_title：Journal of bacteriology

authors： Dul MJ,Young FE

更新日期：1973-09-01 00:00:00

abstract：:Bacterial chemotaxis is based on modulation of the probability to switch the direction of flagellar rotation. Responses to many stimuli are transduced by a two-component system via reversible phosphorylation of CheY, a small cytoplasmic protein that directly interacts with the switch complex at the flagellar motor. We...

journal_title：Journal of bacteriology

authors： Montrone M,Oesterhelt D,Marwan W

更新日期：1996-12-01 00:00:00

abstract：:Granular structures containing inorganic polyphosphate were found in Micrococcus lysodeikticus. These structures were isolated by fractionation of the bacterial extract obtained by lysing the organisms with lysozyme. The composition of the fraction which was enriched with these structures was found to be: protein, 24%...

journal_title：Journal of bacteriology

authors： Friedberg I,Avigad G

更新日期：1968-08-01 00:00:00

abstract：:The chromosomal DNA replication origins (oriC) from two members of the family Enterobacteriaceae, Enterobacter aerogenes and Klebsiella pneumoniae, have been isolated as functional replication origins in Escherichia coli. The origins in the SalI restriction fragments of 17.5 and 10.2 kilobase pairs, cloned from E. aer...

journal_title：Journal of bacteriology

authors： Harding NE,Cleary JM,Smith DW,Michon JJ,Brusilow WS,Zyskind JW

更新日期：1982-12-01 00:00:00

abstract：:Relatively high transfo mation frequencies were obtained with Micrococcus lysodeikticus cells, previou[ill]sly grown in defined broth, with a tris(hydroxymethyl)-aminomethane-glutamate b uffer containing Mg(++), Ca(++), Ba(++), or Sr(++). ...

journal_title：Journal of bacteriology

authors： Kloos WE

更新日期：1969-06-01 00:00:00

abstract：:Sinorhizobium meliloti is a free-living soil bacterium which is capable of establishing a symbiotic relationship with the alfalfa plant (Medicago sativa). This symbiosis involves a network of bacterium-host signaling, as well as the potential for bacterium-bacterium communication, such as quorum sensing. In this study...

journal_title：Journal of bacteriology

authors： Marketon MM,González JE

更新日期：2002-07-01 00:00:00

abstract：:The inducible temperate bacteriophage phi75 and a clear-plaque-forming variant, phi75C1, mediated transduction of a 4.4 X 10(6)-dalton multicopy Bacillus pumilus plasmid, pPL10, at frequencies of 10(-5) to 10(-6) transductants per plaque-forming unit. phi75- and phi75C1-mediated transduction of several chromosome mark...

journal_title：Journal of bacteriology

authors： Bramucci MG,Lovett PS

更新日期：1977-09-01 00:00:00

abstract：:We have characterized an acyl carrier protein (ACP) presumed to be involved in the synthesis of fatty acids in Streptomyces coelicolor A3(2). This is the third ACP to have been identified in S. coelicolor; the two previously characterized ACPs are involved in the synthesis of two aromatic polyketides: the blue-pigment...

journal_title：Journal of bacteriology

authors： Revill WP,Bibb MJ,Hopwood DA

更新日期：1996-10-01 00:00:00

abstract：:In bacteria, the 4-amino-hydroxymethyl-2-methylpyrimidine (HMP) moiety of thiamine is synthesized from 5-aminoimidazole ribotide (AIR), a branch point metabolite of purine and thiamine biosynthesis. ThiC is a member of the radical S-adenosylmethionine (AdoMet) superfamily and catalyzes the complex chemical rearrangeme...

journal_title：Journal of bacteriology

authors： Palmer LD,Dougherty MJ,Downs DM

更新日期：2012-11-01 00:00:00

abstract：:Exposure of Escherichia coli strains deficient in molybdopterin biosynthesis (moa) to the purine base N-6-hydroxylaminopurine (HAP) is mutagenic and toxic. We show that moa mutants exposed to HAP also exhibit elevated mutagenesis, a hyperrecombination phenotype, and increased SOS induction. The E. coli rdgB gene encod...

journal_title：Journal of bacteriology

authors： Burgis NE,Brucker JJ,Cunningham RP

更新日期：2003-05-01 00:00:00

abstract：:The nucleotide sequence of the plasmid-encoded LlaKR2I restriction-modification (R-M) system of Lactococcus lactis subsp. lactis biovar diacetylactis KR2 was determined. This R-M system comprises divergently transcribed endonuclease (llaKR2IR) and methyltransferase (llaKR2IM) genes; located in the intergenic region is...

journal_title：Journal of bacteriology

authors： Twomey DP,McKay LL,O'Sullivan DJ

更新日期：1998-11-01 00:00:00

abstract：:Methylation of DNA at the C-5 position of cytosine occurs in diverse organisms. This modification can increase the rate of C→T transitions at the methylated position. In Escherichia coli and related enteric bacteria, the inner C residues of the sequence CCWGG (W is A or T) are methylated by the Dcm enzyme. These sites...

journal_title：Journal of bacteriology

authors： Cherry JL

更新日期：2018-11-26 00:00:00

abstract：:Herman, Alberta (University of Wisconsin, Madison) and Harlyn Halvorson. Identification of the structural gene for beta-glucosidase in Saccharomyces lactis. J. Bacteriol. 85:895-900. 1963.-Three allelic forms (B(h, m, l)) of the structural gene for beta-glucosidase have been identified in the yeast Saccharomyces lacti...

journal_title：Journal of bacteriology

authors： HERMAN A,HALVORSON H

更新日期：1963-04-01 00:00:00

abstract：:RecAX53 is a chimeric variant of the Escherichia coli RecA protein (RecAEc) that contains a part of the central domain of Pseudomonas aeruginosa RecA (RecAPa), encompassing a region that differs from RecAEc at 12 amino acid positions. Like RecAPa, this chimera exhibits hyperrecombination activity in E. coli cells, inc...

journal_title：Journal of bacteriology

authors： Baitin DM,Bakhlanova IV,Chervyakova DV,Kil YV,Lanzov VA,Cox MM

更新日期：2008-04-01 00:00:00

abstract：:The hook-basal body complex of Salmonella typhimurium, a major component of its flagellar apparatus, was subjected to detailed analysis by electron microscopy and gel electrophoresis. The study was facilitated by the development of an improved protocol for isolation of the complexes in high yield and purity. Nine prot...

journal_title：Journal of bacteriology

authors： Aizawa SI,Dean GE,Jones CJ,Macnab RM,Yamaguchi S

更新日期：1985-03-01 00:00:00

abstract：:In response to iron limitation, the siderophore enterobactin is synthesized and secreted by Escherichia coli. Its biosynthesis is performed by a series of enzymes encoded by the Ent gene cluster. Among the genes of this cluster, ybdB has not been implicated in enterobactin production to date. We demonstrate here an in...

journal_title：Journal of bacteriology

authors： Leduc D,Battesti A,Bouveret E

更新日期：2007-10-01 00:00:00

abstract：:The cold shock response of Escherichia coli is elicited by downshift of temperature from 37 degrees C to 15 degrees C and is characterized by induction of several cold shock proteins, including CsdA, during the acclimation phase. CsdA, a DEAD-box protein, has been proposed to participate in a variety of processes, suc...

journal_title：Journal of bacteriology

authors： Awano N,Xu C,Ke H,Inoue K,Inouye M,Phadtare S

更新日期：2007-08-01 00:00:00

abstract：:Expression of the Escherichia coli OmpC and OmpF outer membrane proteins is regulated by the osmolarity of the culture media. In contrast, expression of OmpC in Salmonella typhi is not influenced by osmolarity, while OmpF is regulated as in E. coli. To better understand the lack of osmoregulation of OmpC expression in...

journal_title：Journal of bacteriology

authors： Martínez-Flores I,Cano R,Bustamante VH,Calva E,Puente JL

更新日期：1999-01-01 00:00:00

abstract：:Respiratory nitrate reductase purified from the cell membrane of Escherichia coli is composed of three subunits, alpha, beta, and gamma, which are encoded, respectively, by the narG, narH, and narI genes of the narGHJI operon. The product of the narJ gene was deduced previously to be a highly charged, acidic protein w...

journal_title：Journal of bacteriology

authors： Dubourdieu M,DeMoss JA

更新日期：1992-02-01 00:00:00

abstract：:We have used a plasmid-based transcriptional reporter system to examine the transcriptional effects of 33 single point mutations in the box A region (TATA-like sequence) of the Haloferax volcanii tRNA(Lys) promoter. The most pronounced effects on transcriptional efficiency were found when the nucleotides corresponding...

journal_title：Journal of bacteriology

authors： Palmer JR,Daniels CJ

更新日期：1995-04-01 00:00:00

abstract：:The ultrastructure of the cytoplasmic membrane and cell wall of two strains of Escherichia coli, Proteus morganii, P. vulgaris, Acinetobacter anitratum, Moraxella lacunata, Erwinia amylovora, Acinetobacter sp., and of a plant pathogen, unclassified gram-negative, fixed by the Ryter-Kellenberger procedure, was found to...

journal_title：Journal of bacteriology

authors： Silva MT,Sousa JC

更新日期：1973-02-01 00:00:00

abstract：:The ribG gene at the 5' end of the riboflavin operon of Bacillus subtilis and a reading frame at 442 kb on the Escherichia coli chromosome (subsequently designated ribD) show similarity with deoxycytidylate deaminase and with the RIB7 gene of Saccharomyces cerevisiae. The ribG gene of B. subtilis and the ribD gene of ...

journal_title：Journal of bacteriology

authors： Richter G,Fischer M,Krieger C,Eberhardt S,Lüttgen H,Gerstenschläger I,Bacher A

更新日期：1997-03-01 00:00:00

abstract：:Cnm is a surface-associated protein present in a subset of Streptococcus mutans strains that mediates binding to extracellular matrices, intracellular invasion, and virulence. Here, we showed that cnm transcription is controlled by the global regulators CovR and VicRKX. In silico analysis identified multiple putative ...

journal_title：Journal of bacteriology

authors： Araújo Alves L,Ganguly T,Mattos-Graner RO,Kajfasz J,Harth-Chu EN,Lemos JA,Abranches J

更新日期：2018-11-06 00:00:00

abstract：:Five temperature-sensitive lysis mutants were found to possess very low diaminopimelic acid (Dpm) adding enzyme activity in vitro. Murein synthesis at 42 C was impaired, and uridine-5'-diphosphate-N-acetyl-muramyl-l-Ala- d-Glu (UDP-MurNAc-dipeptide) was accumulated. In the presence of NaCl, the mutants could grow at 4...

journal_title：Journal of bacteriology

authors： Lugtenberg EJ,v Schijndel-van Dam A

更新日期：1972-04-01 00:00:00

abstract：:During development of the symbiotic soil bacterium Sinorhizobium meliloti into nitrogen-fixing bacteroids, DNA replication and cell division cease and the cells undergo profound metabolic and morphological changes. Regulatory genes controlling the early stages of this process have not been identified. As a first step ...

journal_title：Journal of bacteriology

authors： Barnett MJ,Hung DY,Reisenauer A,Shapiro L,Long SR

更新日期：2001-05-01 00:00:00