Abstract:
:In bacteria, the 4-amino-hydroxymethyl-2-methylpyrimidine (HMP) moiety of thiamine is synthesized from 5-aminoimidazole ribotide (AIR), a branch point metabolite of purine and thiamine biosynthesis. ThiC is a member of the radical S-adenosylmethionine (AdoMet) superfamily and catalyzes the complex chemical rearrangement of AIR to HMP-P. As reconstituted in vitro, the ThiC reaction requires AdoMet, AIR, and reductant. This study analyzed variants of ThiC in vivo and in vitro to probe the metabolic network surrounding AIR in Salmonella enterica. Several variants of ThiC that required metabolic perturbations to function in vivo were biochemically characterized in vitro. Results presented herein indicate that the subtleties of the metabolic network have not been captured in the current reconstitution of the ThiC reaction.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Palmer LD,Dougherty MJ,Downs DMdoi
10.1128/JB.01361-12subject
Has Abstractpub_date
2012-11-01 00:00:00pages
6088-95issue
22eissn
0021-9193issn
1098-5530pii
JB.01361-12journal_volume
194pub_type
杂志文章abstract::The gcaD, prs, and ctc genes were shown to be organized as a tricistronic operon. The transcription of the prs gene, measured as phosphoribosyl diphosphate synthetase activity, and of the ctc gene, measured as beta-galactosidase activity specified by a ctc-lacZ protein fusion, were dependent on the promoter in front o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.24.7280-7284.1995
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doi:10.1128/JB.159.1.167-172.1984
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journal_title:Journal of bacteriology
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doi:10.1128/jb.176.11.3400-3402.1994
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.142.1.366-368.1980
更新日期:1980-04-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1997-04-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.4.1417-1422.1988
更新日期:1988-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.2.1056-1058.1982
更新日期:1982-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.2.631-639.1975
更新日期:1975-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.2.763-769.1977
更新日期:1977-02-01 00:00:00
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更新日期:2001-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.22.6959-6964.1997
更新日期:1997-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.128.1.363-371.1976
更新日期:1976-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2005-04-01 00:00:00
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doi:10.1128/jb.176.15.4501-4510.1994
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pub_type: 杂志文章
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更新日期:1986-10-01 00:00:00
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更新日期:1962-10-01 00:00:00
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更新日期:2009-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1980-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.3.1141-1156.1978
更新日期:1978-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2007-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2007-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.16.4870-4876.1996
更新日期:1996-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.3.750-757.1995
更新日期:1995-02-01 00:00:00