Abstract:
:We isolated and characterized crp mutations in Escherichia coli that allow cyclic AMP (cAMP) receptor protein to function without cAMP. These mutants defined a region involved in the cAMP-induced allosteric change of cAMP receptor protein that is necessary for activation of the protein. Currently, we have isolated intragenic suppressors of the crp mutations. These crp (Sup) mutants require cAMP for activity. The crp (Sup) mutations map in regions which define new sites of changes involved in cAMP receptor protein activation. From these results, we suggest that to activate cAMP receptor protein cAMP brings about (i) a hinge reorientation to eject the DNA-binding F alpha-helices, (ii) proper alignment between the two subunits, and (iii) an adjustment between the position of the two domains. Cyclic GMP fails to effect the last step.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Garges S,Adhya Sdoi
10.1128/jb.170.4.1417-1422.1988subject
Has Abstractpub_date
1988-04-01 00:00:00pages
1417-22issue
4eissn
0021-9193issn
1098-5530journal_volume
170pub_type
杂志文章abstract::The transglycosylation step of cell wall synthesis is a prime antibiotic target because it is essential and specific to bacteria. Two antibiotics, ramoplanin and moenomycin, target this step by binding to the substrate lipid II and the transglycosylase enzyme, respectively. Here, we compare the ramoplanin and moenomyc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05862-11
更新日期:2011-10-01 00:00:00
abstract::The region upstream of the Escherichia coli bgl operon is an insertion hot spot for several transposons. Elements as distantly related as Tn1, Tn5, and phage Mu home in on this location. To see what characteristics result in a high-affinity site for transposition, we compared in vivo and in vitro Mu transposition patt...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.11.3328-3335.2001
更新日期:2001-06-01 00:00:00
abstract::Salmonella typhimurium contains three "major proteins" or "porins" (34K, 35K, and 36K) in the outer membrane. A mutant strain producing only the 35K porin was first grown in media containing high concentrations of NaCl to "repress" the porin synthesis and then was shifted into a medium without NaCl. The newly made por...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.2.687-702.1978
更新日期:1978-08-01 00:00:00
abstract::The specific activities of 13 aminoacyl-soluble ribonucleic acid (sRNA) synthetases were measured at various time intervals during the germination of Botryodiplodia theobromae conidiospores. The enzyme activities were low or absent in ungerminated spores, and they increased rapidly as germination proceeded. When extra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.4.1042-1048.1968
更新日期:1968-10-01 00:00:00
abstract::Mycoplasmas isolated from simian (Cercopithecus aethiops) tissues were shown to have biological, biochemical, and serological properties and electrophoretic cell protein patterns similar to strain Navel isolated from man 15 years ago. The simian and human Navel strains comprised a single serogroup, distinct from the e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.1.439-445.1971
更新日期:1971-10-01 00:00:00
abstract::A mutant (designated mec(-)) of Escherichia coli F(+) 100 endo I(-)su(+) r(K) (-)m(K) (+) has been isolated which is defective in cytosine-specific deoxyribonucleic acid (DNA) methylase activity. The DNA of this mutant, as well as the DNA of phages lambda and fd propagated in it, is virtually devoid of 5-methyl-cytosi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.115.3.1103-1107.1973
更新日期:1973-09-01 00:00:00
abstract::The messenger ribonucleic acid (mRNA) pattern of log-phase and sporulating cells of Bacillus subtilis has been analyzed by deoxyribonucleic acid (DNA)-RNA hybrid studies with the complementary-strand fragments of DNA. Approximately 80% of the mRNA of log-phase and sporulating cells from stages I, III, and IV hybridize...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.117.2.775-782.1974
更新日期:1974-02-01 00:00:00
abstract::DNA sequence analysis of a region of the Streptomyces sp. strain C5 daunomycin biosynthesis gene cluster, located between the daunomycin polyketide biosynthesis gene cluster and a dnrI (transcriptional activator) homolog, revealed the presence of a gene encoding a P-450-like enzyme with a deduced Mr of 46,096. Express...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.11.3389-3395.1996
更新日期:1996-06-01 00:00:00
abstract::Hedgecock, L. W. (Veterans Administration Hospital, Kansas City, Mo.) and R. L. Costello. Utilization of nitrate by pathogenic and saprophytic mycobacteria. J. Bacteriol. 84:195-205. 1962-The ability of mycobacteria to utilize nitrate as a sole source of nitrogen was examined. Nitrate-nitrogen was readily utilized by ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.2.195-205.1962
更新日期:1962-08-01 00:00:00
abstract::Streptococcus oralis, a commensal species of the human oral cavity, belongs to the Mitis group of streptococci, which includes one of the major human pathogens as well, S. pneumoniae. We report here the first complete genome sequence of this species. S. oralis Uo5, a high-level penicillin- and multiple-antibiotic-resi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00321-11
更新日期:2011-06-01 00:00:00
abstract::The WalRK two-component system plays important roles in maintaining cell wall homeostasis and responding to antibiotic stress in low-GC Gram-positive bacteria. In the major human pathogen, Streptococcus pneumoniae, phosphorylated WalR(Spn) (VicR) response regulator positively controls the transcription of genes encodi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01690-09
更新日期:2010-05-01 00:00:00
abstract::Isoaspartyl sites, in which an aspartic acid residue is linked to its C-flanking neighbor via its beta-carboxyl side chain, are generally assumed to be an abnormal modification arising as proteins age. The enzyme protein L-isoaspartate methyltransferase (PIMT), present in many bacteria, plants, and animals, catalyzes ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.9.2872-2877.1999
更新日期:1999-05-01 00:00:00
abstract::Sulfolobus sulfataricus ATCC 35091, Haloferax volcanii, and Methanosarcina thermophila TM-1, representing the Euryarchaeota and Crenarchaeota subdomains of the Archaea, contain proteins which are phosphorylated on tyrosine. These data raise fundamental questions as to the origin and evolution of tyrosine phosphorylati...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.7.2418-2420.1997
更新日期:1997-04-01 00:00:00
abstract::Benyesh-Melnick, Matilda (Baylor University College of Medicine, Houston, Tex.), Fern Probstmeyer, Robert McCombs, Jean P. Brunschwig, and Vladimir Vonka. Correlation between infectivity and physical virus particles in human cyto-megalovirus. J. Bacteriol. 92:1555-1561. 1966.-Infectivity titers [measured as plaque-for...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.5.1555-1561.1966
更新日期:1966-11-01 00:00:00
abstract::Coliphage TuIa, which uses the OmpF protein as a receptor, can adapt by mutation to using instead the OmpC or LamB protein, or both. Most of the phage mutants retained the ability to use the OmpF protein, when present, but one class of mutants lost this ability and could only use the OmpC protein. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.3.1182-1185.1980
更新日期:1980-12-01 00:00:00
abstract::Copper (Cu) is a required micronutrient, but it is highly toxic at high concentrations. Therefore, the levels of Cu must be tightly regulated in all living cells. The phagosome of Mycobacterium tuberculosis has been shown to have variable levels of Cu. Previously, we showed that M. tuberculosis contains a copper-sensi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01847-07
更新日期:2008-04-01 00:00:00
abstract::Upon exposure to alkylating agents, Escherichia coli increases expression of aidB along with three genes (ada, alkA, and alkB) that encode DNA repair proteins. While the biological roles of the Ada, AlkA, and AlkB proteins have been defined, despite many efforts, the molecular functions of AidB remain largely unknown....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00858-10
更新日期:2010-12-01 00:00:00
abstract::A comprehensive equation, upsilon = VM/[1 + (A0.5/Fru-P2)n] [ 1 + (Glc-6-P/I0.5)], has been proposed to represent the quantitative interrelationships between the rate of glucose utilization and the levels of glucose-6-phosphate and fructose-1,6-diphosphate in the intact Escherichia coli cell. This comprehensive equati...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.128.1.165-169.1976
更新日期:1976-10-01 00:00:00
abstract::Bacteria able to use at least one of 13 ionic alkylphosphonates of O-alkyl or O,O-dialkyl alkylphosphonates as phosphorus sources were isolated from sewage and soil. Four of these isolates used 2-aminoethylphosphonic acid (AEP) as a sole carbon, nitrogen, and phosphorus source. None of the other phosphonates served as...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.1.85-90.1978
更新日期:1978-01-01 00:00:00
abstract::The filamentous cyanobacterium Anabaena (Nostoc) sp. strain PCC 7120 produces specialized cells for nitrogen fixation called heterocysts. Previous work showed that the group 2 sigma factor sigE (alr4249; previously called sigF) is upregulated in differentiating heterocysts 16 h after nitrogen step-down. We now show th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01472-10
更新日期:2011-04-01 00:00:00
abstract::Two distinct spore appendage types of Clostridium bifermentans, a pinlike appendage and a tubular appendage, were studied by electron microscopy. The pinlike appendage is characterized by a shaft, about 100 A in diameter, which has a lobed caplike structure. The tubular appendage, 500 to 600 A in diameter, is characte...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.1.231-238.1968
更新日期:1968-01-01 00:00:00
abstract::Guanosine tetraphosphate (ppGpp) is a key mediator of stringent control, an adaptive response of bacteria to amino acid starvation, and has thus been termed a bacterial alarmone. Previous X-ray crystallographic analysis has provided a structural basis for the transcriptional regulation of RNA polymerase activity by pp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00574-06
更新日期:2006-10-01 00:00:00
abstract::Phosphatidylglycerol, the most abundant acidic phospholipid in Escherichia coli, is considered to play specific roles in various cellular processes that are essential for cell viability. A null mutation of pgsA, which encodes phosphatidylglycerophosphate synthase, does indeed confer lethality. However, pgsA null mutan...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.19.5418-5425.2002
更新日期:2002-10-01 00:00:00
abstract::Hall, Herbert E. (Robert A. Taft Sanitary Engineering Center, Cincinnati, Ohio), Robert Angelotti, Keith H. Lewis, and Milton J. Foter. Characteristics of Clostridium perfringens strains associated with food and food-borne disease. J. Bacteriol. 85:1094-1103. 1963.-A total of 83 strains of Clostridium perfringens-30 f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.5.1094-1103.1963
更新日期:1963-05-01 00:00:00
abstract::Rickettsia typhi, the causative agent of murine typhus, is an obligate intracellular bacterium with a life cycle involving both vertebrate and invertebrate hosts. Here we present the complete genome sequence of R. typhi (1,111,496 bp) and compare it to the two published rickettsial genome sequences: R. prowazekii and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.17.5842-5855.2004
更新日期:2004-09-01 00:00:00
abstract::Deoxyribonucleic acid (DNA) reassociation analyses were employed to determine the molecular relationships between recombinable nocardiae. Analysis of the compatibility system of Nocardia erythropolis Mat-Ce and Mat-cE mating strains demonstrated the existence of extensive homology under both exacting and nonexacting c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.109.2.720-729.1972
更新日期:1972-02-01 00:00:00
abstract::The Escherichia coli purine repressor, PurR, binds to a 16-bp operator sequence and coregulates the genes for de novo synthesis of purine and pyrimidine nucleotides, formation of a one-carbon unit for biosynthesis, and deamination of cytosine. We have characterized the purified repressor. Chemical cross-linking indica...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.19.6207-6214.1992
更新日期:1992-10-01 00:00:00
abstract::Reyranella massiliensis is an Alphaproteobacterium member of the class Rhodospirillaceae, growing in amoebae. We sequenced the genome of type strain 521(T). It is composed of a 5,792,218-bp chromosome and encodes 5,675 protein-coding genes and 53 RNA genes, including 3 rRNA genes. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01260-12
更新日期:2012-10-01 00:00:00
abstract::Proteins synthesized in Escherichia coli during recovery from starvation were resolved by two-dimensional polyacrylamide gel electrophoresis. Nine outgrowth-specific proteins, which appeared in two kinetic groups, that were not detected in either starved or exponential-phase cells were synthesized. Five other proteins...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.21.6352-6356.1996
更新日期:1996-11-01 00:00:00
abstract::In the thermophilic archaeon Methanobacterium thermoautotrophicum Marburg, the structural gene for isoleucyl-tRNA synthetase (ileS) is flanked upstream by orf401 and downstream by purL. orf401 encodes a 43.5-kDa protein with an unknown function. Northern (RNA) hybridization and S1 nuclease protection experiments showe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.18.5945-5952.1993
更新日期:1993-09-01 00:00:00