The sigE gene is required for normal expression of heterocyst-specific genes in Anabaena sp. strain PCC 7120.

Abstract:

:The filamentous cyanobacterium Anabaena (Nostoc) sp. strain PCC 7120 produces specialized cells for nitrogen fixation called heterocysts. Previous work showed that the group 2 sigma factor sigE (alr4249; previously called sigF) is upregulated in differentiating heterocysts 16 h after nitrogen step-down. We now show that the sigE gene is required for normal heterocyst development and normal expression levels of several heterocyst-specific genes. Mobility shift assays showed that the transcription factor NtcA binds to sites in the upstream region of sigE and that this binding is enhanced by 2-oxoglutarate (2-OG). Deletions of the region containing the NtcA binding sites in P(sigE)-gfp reporter plasmids showed that the sites contribute to normal developmental regulation but are not essential for upregulation in heterocysts. Northern RNA blot analysis of nifH mRNA revealed delayed and reduced transcript levels during heterocyst differentiation in a sigE mutant background. Quantitative reverse transcription-PCR (qRT-PCR) analyses of the sigE mutant showed lower levels of transcripts for nifH, fdxH, and hglE2 but normal levels for hupL. We developed a P(nifHD)-gfp reporter construct that showed strong heterocyst-specific expression. Time-lapse microscopy of the P(nifHD)-gfp reporter in a sigE mutant background showed delayed development and undetectable green fluorescent protein (GFP) fluorescence. Overexpression of sigE caused accelerated heterocyst development, an increased heterocyst frequency, and premature expression of GFP fluorescence from the P(nifHD)-gfp reporter.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Mella-Herrera RA,Neunuebel MR,Kumar K,Saha SK,Golden JW

doi

10.1128/JB.01472-10

subject

Has Abstract

pub_date

2011-04-01 00:00:00

pages

1823-32

issue

8

eissn

0021-9193

issn

1098-5530

pii

JB.01472-10

journal_volume

193

pub_type

杂志文章
  • Molecular analysis and regulation of the glnA gene of the gram-positive anaerobe Clostridium acetobutylicum.

    abstract::The nucleotide sequence of a 2.0-kilobase DNA segment containing the Clostridium acetobutylicum glnA gene was determined. The upstream region of the glnA gene contained two putative extended promoter consensus sequences (p1 and p2), characteristic of gram-positive bacteria. A third putative extended gram-positive prom...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.1.400-408.1988

    authors: Janssen PJ,Jones WA,Jones DT,Woods DR

    更新日期:1988-01-01 00:00:00

  • Clostridium perfringens spore germination: characterization of germinants and their receptors.

    abstract::Clostridium perfringens food poisoning is caused by type A isolates carrying a chromosomal enterotoxin (cpe) gene (C-cpe), while C. perfringens-associated non-food-borne gastrointestinal (GI) diseases are caused by isolates carrying a plasmid-borne cpe gene (P-cpe). C. perfringens spores are thought to be the importan...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01748-07

    authors: Paredes-Sabja D,Torres JA,Setlow P,Sarker MR

    更新日期:2008-02-01 00:00:00

  • The concentration of ammonia regulates nitrogen metabolism in Saccharomyces cerevisiae.

    abstract::Saccharomyces cerevisiae was grown in a continuous culture at a single dilution rate with input ammonia concentrations whose effects ranged from nitrogen limitation to nitrogen excess and glucose limitation. The rate of ammonia assimilation (in millimoles per gram of cells per hour) was approximately constant. Increas...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.22.6672-6675.1995

    authors: ter Schure EG,Silljé HH,Verkleij AJ,Boonstra J,Verrips CT

    更新日期:1995-11-01 00:00:00

  • The Saccharomyces cerevisiae Isw2p-Itc1p complex represses INO1 expression and maintains cell morphology.

    abstract::In the yeast Saccharomyces cerevisiae, IRE1 encodes a bifunctional protein with transmembrane kinase and endoribonuclease activities. HAC1 encodes a transcription factor which has a basic leucine zipper domain. Both gene products play a crucial role in the unfolded protein response. Mutants in which one of these genes...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.183.17.4985-4993.2001

    authors: Sugiyama M,Nikawa J

    更新日期:2001-09-01 00:00:00

  • Gene transfer to the desiccation-tolerant cyanobacterium Chroococcidiopsis.

    abstract::The coccoid cyanobacterium Chroococcidiopsis dominates microbial communities in the most extreme arid hot and cold deserts. These communities withstand constraints that result from multiple cycles of drying and wetting and/or prolonged desiccation, through mechanisms which remain poorly understood. Here we describe th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.7.2298-2305.2001

    authors: Billi D,Friedmann EI,Helm RF,Potts M

    更新日期:2001-04-01 00:00:00

  • Transcriptional induction kinetics from the promoters of the catabolic pathways of TOL plasmid pWW0 of Pseudomonas putida for metabolism of aromatics.

    abstract::We determined, under several growth conditions, the kinetics of mRNA synthesis from the four Pseudomonas putida pWW0 plasmid promoters involved in degradation of xylenes and methylbenzyl alcohols via toluates. Transcription by XylS of the meta-cleavage pathway operon promoter (Pm) for the metabolism of alkylbenzoates ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.9.2517-2524.1994

    authors: Marqués S,Holtel A,Timmis KN,Ramos JL

    更新日期:1994-05-01 00:00:00

  • Conjugative plasmids of enteric bacteria from many different incompatibility groups have similar genes for single-stranded DNA-binding proteins.

    abstract::Among 30 conjugative plasmids of enteric bacteria from 23 incompatibility (Inc) groups, we found 19 (from 12 Inc groups) which can complement defects caused by a defective single-stranded DNA-binding protein of Escherichia coli K-12. The genes which are responsible for the complementation from three of these plasmids ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.1.235-241.1985

    authors: Golub EI,Low KB

    更新日期:1985-04-01 00:00:00

  • Interactive regulation of Azorhizobium nifA transcription via overlapping promoters.

    abstract::The Azorhizobium nifA promoter (PnifA) is positively regulated by two physiological signal transduction pathways, NtrBC, which signals anabolic N status, and FixLJK, which signals prevailing O2 status. Yet, PnifA response (gene product per unit time) to these two activating signals together is more than twice that of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.24.7210-7221.1995

    authors: Loroch AI,Nguyen BG,Ludwig RA

    更新日期:1995-12-01 00:00:00

  • Constancy of growth on simple and complex media.

    abstract::An apparatus has been developed in which bacterial growth can be measured very precisely over short intervals of time. Its precision is presented and used to assess the constancy of growth in batch culture. Under certain conditions, i.e., Luria broth or 0.2% glucose-M9 medium at very low cell densities, the specific g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.136.3.969-975.1978

    authors: Wang CH,Koch AL

    更新日期:1978-12-01 00:00:00

  • Gene order of the TOL catabolic plasmid upper pathway operon and oxidation of both toluene and benzyl alcohol by the xylA product.

    abstract::TOL plasmid pWW0 specifies enzymes for the oxidative catabolism of toluene and xylenes. The upper pathway converts the aromatic hydrocarbons to aromatic carboxylic acids via corresponding alcohols and aldehydes and involves three enzymes: xylene oxygenase, benzyl alcohol dehydrogenase, and benzaldehyde dehydrogenase. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.167.2.455-461.1986

    authors: Harayama S,Leppik RA,Rekik M,Mermod N,Lehrbach PR,Reineke W,Timmis KN

    更新日期:1986-08-01 00:00:00

  • Oxygen-mediated regulation of porphobilinogen formation in Rhodobacter capsulatus.

    abstract::A Rhodobacter capsulatus hemC mutant has been isolated and used to show that oxygen regulates the intracellular levels of porphobilinogen. Experiments using a hemB-cat gene fusion demonstrated that oxygen does not transcriptionally regulate hemB transcription. Porphobilinogen synthase activity is not regulated by oxyg...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.6.1685-1692.2002

    authors: Biel AJ,Canada K,Huang D,Indest K,Sullivan K

    更新日期:2002-03-01 00:00:00

  • AGT1, encoding an alpha-glucoside transporter involved in uptake and intracellular accumulation of trehalose in Saccharomyces cerevisiae.

    abstract::The trehalose content in Saccharomyces cerevisiae can be significantly manipulated by including trehalose at an appropriate level in the growth medium. Its uptake is largely dependent on the expression of AGT1, which encodes an alpha-glucoside transporter. The trehalose found in a tps1 mutant of trehalose synthase may...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.12.3830-3832.1999

    authors: Plourde-Owobi L,Durner S,Parrou JL,Wieczorke R,Goma G,François J

    更新日期:1999-06-01 00:00:00

  • Antibacterial nitroacridine, Nitroakridin 3582: binding to nucleic acids in vitro and effects on selected cell-free model systems of macromolecular biosynthesis.

    abstract::Nitroakridin 3582 (NA) formed complexes with native deoxyribonucleic acid (DNA) and with transfer ribonucleic acid (tRNA) species from Escherichia coli. Spectrophotometric titrations of NA with these nucleic acids produced numerical results from which nonlinear adsorption isotherms were derived. These curves indicated...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.108.3.1026-1033.1971

    authors: Wolfe AD,Cook TM,Hahn FE

    更新日期:1971-12-01 00:00:00

  • The final step of peptidoglycan subunit assembly in Escherichia coli occurs in the cytoplasm.

    abstract::The murG gene of Escherichia coli encodes the N-acetylglucosaminyltransferase responsible for the final step in the formation of the lipid-linked disaccharide-pentapeptide subunit of peptidoglycan. Using trypsin to probe maxicell spheroplasts, we show that this enzyme is peripherally associated with the inner face of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.6.1841-1843.1993

    authors: Bupp K,van Heijenoort J

    更新日期:1993-03-01 00:00:00

  • Expression of the iron-activated nspA and secY genes in Neisseria meningitidis group B by Fur-dependent and -independent mechanisms.

    abstract::Our whole-genome microarray studies of Neisseria meningitidis MC58 previously identified a set of 153 genes whose transcription was activated during growth in iron. In this study, Fur-mediated regulation of the iron-activated nspA gene was confirmed, whereas iron-activated regulation of the secY gene was demonstrated ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01638-06

    authors: Shaik YB,Grogan S,Davey M,Sebastian S,Goswami S,Szmigielski B,Genco CA

    更新日期:2007-01-01 00:00:00

  • Residual polarity and transcription-translation coupling during recovery from chloramphenicol or fusidic acid.

    abstract::Fusidic acid or chloramphenicol was used to inhibit peptide synthesis to 1% of normal in Escherichia coli B, strain AS19. After 10 min of inhibition, peptide synthesis could be quickly restored to 80% of the normal rate after washing the bacteria on a filter. However, even in the presence of adenosine 3'-5'-cyclic-mon...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.117.2.631-640.1974

    authors: Pastushok C,Kennell D

    更新日期:1974-02-01 00:00:00

  • Enterohemolysin production is associated with a temperate bacteriophage in Escherichia coli serogroup O26 strains.

    abstract::A temperate bacteriophage that determines the expression of enterohemolysin was isolated from Escherichia coli O26 strain C3888. The genetic determinant associated with enterohemolysin production (E-Hly determinant) was cloned from EcoRI-digested bacteriophage DNA in vector plasmid pUC8. pUC8 recombinant plasmid pEO19...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.11.6469-6475.1990

    authors: Beutin L,Bode L,Ozel M,Stephan R

    更新日期:1990-11-01 00:00:00

  • The acrAB homolog of Haemophilus influenzae codes for a functional multidrug efflux pump.

    abstract::Disruption of gene HI0894 or HI0895 in Haemophilus influenzae Rd, homologs of Escherichia coli acrAB multidrug efflux genes, caused hypersusceptibility to erythromycin, rifampin, novobiocin, and dyes such as ethidium bromide and crystal violet and increased accumulation of radioactive erythromycin, showing that these ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.21.6855-6857.1997

    authors: Sánchez L,Pan W,Viñas M,Nikaido H

    更新日期:1997-11-01 00:00:00

  • Interactions between the lipoprotein PilP and the secretin PilQ in Neisseria meningitidis.

    abstract::Neisseria meningitidis can be the causative agent of meningitis or septicemia. This bacterium expresses type IV pili, which mediate a variety of functions, including autoagglutination, twitching motility, biofilm formation, adherence, and DNA uptake during transformation. The secretin PilQ supports type IV pilus extru...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00060-07

    authors: Balasingham SV,Collins RF,Assalkhou R,Homberset H,Frye SA,Derrick JP,Tønjum T

    更新日期:2007-08-01 00:00:00

  • Genome sequence of Reyranella massiliensis, a bacterium associated with amoebae.

    abstract::Reyranella massiliensis is an Alphaproteobacterium member of the class Rhodospirillaceae, growing in amoebae. We sequenced the genome of type strain 521(T). It is composed of a 5,792,218-bp chromosome and encodes 5,675 protein-coding genes and 53 RNA genes, including 3 rRNA genes. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01260-12

    authors: Pagnier I,Croce O,Robert C,Raoult D,La Scola B

    更新日期:2012-10-01 00:00:00

  • Proteins of the inner membrane of Escherichia coli: identification of succinate dehydrogenase by polyacrylamide gel electrophoresis with sdh amber mutants.

    abstract::The inner or cytoplasmic membrane fraction of the cell envelope of Escherichia coli was isolated by isopycnic centrifugation on sucrose gradients. The membrane proteins were analyzed by electrophoresis in sodium dodecyl sulfate-polyacrylamide gels (8.5%), and up to 56 bands were resolved. Different preparations gave v...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.117.3.947-953.1974

    authors: Spencer ME,Guest JR

    更新日期:1974-03-01 00:00:00

  • Nitrogenase activity in cell-free extracts of the blue-green alga, Anabaena cylindrica.

    abstract::Cell-free extracts with high nitrogenase activity were prepared by sonic oscillation and French press treatment from the blue-gree alga Anabaena cylindrica. Extracts were prepared from cells grown on a 95% N(2)-5% CO(2) gas mixture followed by a period of nitrogen starvation under an atmosphere of 95% argon-5% CO(2). ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.3.913-917.1971

    authors: Smith RV,Evans MC

    更新日期:1971-03-01 00:00:00

  • Mobilization of Bacteroides plasmids by Bacteroides conjugal elements.

    abstract::A 4.2-kilobase cryptic Bacteroides plasmid, pB8-51, is found in several colonic Bacteroides species. To determine whether pB8-51 is mobilized by any of the known Bacteroides conjugal elements, we constructed an Escherichia coli-Bacteroides shuttle vector, pVAL-1, which contains pB8-51. We constructed Bacteroides unifo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.3.1319-1324.1988

    authors: Valentine PJ,Shoemaker NB,Salyers AA

    更新日期:1988-03-01 00:00:00

  • Morphogenesis of Candida albicans and cytoplasmic proteins associated with differences in morphology, strain, or temperature.

    abstract::The extent of change in cytoplasmic proteins which accompanies yeast-to-mycelium morphogenesis of Candida albicans was analyzed by two-dimensional gel electrophoresis. Pure cultures of yeasts and true hyphae (i.e., without concomitant production of pseudohyphae) were grown in a synthetic low-sulfate medium. The two st...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.144.1.258-273.1980

    authors: Manning M,Mitchell TG

    更新日期:1980-10-01 00:00:00

  • Intracellular serine protease 1 of Bacillus subtilis is formed in vivo as an unprocessed, active protease in stationary cells.

    abstract::Western immunoblots and assays of Bacillus subtilis extracts showed that intracellular serine protease 1 is produced in a form larger than previously reported, appears not to have undergone N-terminal processing, and is active in the presence or absence of calcium. No evidence for an inactive precursor form of the pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.1.473-476.1990

    authors: Sheehan SM,Switzer RL

    更新日期:1990-01-01 00:00:00

  • A mer-lux transcriptional fusion for real-time examination of in vivo gene expression kinetics and promoter response to altered superhelicity.

    abstract::We constructed mercury resistance operon-luciferase (mer-lux) transcriptional fusion plasmids to evaluate in vivo gene expression rates of the mer structural gene promoter (PTPCAD) of transposon Tn21. In vivo gene expression kinetics corresponded well with those previously determined in vitro, yielding an apparent K0....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.24.8094-8101.1992

    authors: Condee CW,Summers AO

    更新日期:1992-12-01 00:00:00

  • The requirement for pneumococcal MreC and MreD is relieved by inactivation of the gene encoding PBP1a.

    abstract::MreC and MreD, along with the actin homologue MreB, are required to maintain the shape of rod-shaped bacteria. The depletion of MreCD in rod-shaped bacteria leads to the formation of spherical cells and the accumulation of suppressor mutations. Ovococcus bacteria, such as Streptococcus pneumoniae, lack MreB homologues...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05245-11

    authors: Land AD,Winkler ME

    更新日期:2011-08-01 00:00:00

  • Ribonucleic acid synthesis in T2-infected Escherichia coli during "stringent" control.

    abstract::The leucine auxotroph Escherichia coli 2961 exhibited stringent control of net ribonucleic acid (RNA) synthesis during amino acid starvation. After leucine was exhausted from the medium, the rate of uracil incorporation into RNA rapidly decreased to 2 to 4% of the prestarvation value. Infection of the starved cells wi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.1.111-116.1968

    authors: Sharp K,Green MH

    更新日期:1968-07-01 00:00:00

  • MECHANISM OF ACTION OF THE TOXIN OF BACILLUS ANTHRACIS II. : Alkaline Phosphatasemia Produced by Culture Filtrates of Various Bacilli.

    abstract::Slein, Milton W. (Fort Detrick, Frederick, Md.) and Gerald F. Logan, Jr. Mechanism of action of the toxin of Bacillus anthracis. II. Alkaline phosphatasemia produced by culture filtrates of various bacilli. J. Bacteriol. 83:359-369. 1962.-A factor which produces hyperphosphatasemia after intravenous injection into ani...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.2.359-369.1962

    authors: Slein MW,Logan GF

    更新日期:1962-02-01 00:00:00

  • Cloning and expression of the exfoliative toxin B gene from Staphylococcus aureus.

    abstract::Exfoliative toxin type B is produced by bacteriophage group II strains of Staphylococcus aureus and is a causative agent of staphylococcal scalded-skin syndrome. In addition to exfoliative toxin B, most isolates also produce a bacteriocin and are immune to the action of the bacteriocin. These phenotypes, as well as re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.166.2.574-580.1986

    authors: Jackson MP,Iandolo JJ

    更新日期:1986-05-01 00:00:00