Nitrogenase activity in cell-free extracts of the blue-green alga, Anabaena cylindrica.

Abstract:

:Cell-free extracts with high nitrogenase activity were prepared by sonic oscillation and French press treatment from the blue-gree alga Anabaena cylindrica. Extracts were prepared from cells grown on a 95% N(2)-5% CO(2) gas mixture followed by a period of nitrogen starvation under an atmosphere of 95% argon-5% CO(2). No increase in the specific activity of extracts was achieved by breaking heterocysts. Activity (assayed by acetylene reduction) was found to be dependent on adenosine triphosphate (ATP), an ATP-generating system, and a low-potential reductant. Na(2)S(2)O(2) employed as reductant supports higher rates of nitrogenase activity than reduced ferredoxin. The activity is associated with a small-particle fraction that can be sedimented by ultracentrifugation. In contrast to the particulate nitrogenase of Azotobacter, which is stable in air, the A. cylindrica nitrogenase is an oxygen sensitive as nitrogenase prepared from anaerobic bacteria.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Smith RV,Evans MC

doi

10.1128/JB.105.3.913-917.1971

subject

Has Abstract

pub_date

1971-03-01 00:00:00

pages

913-7

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

105

pub_type

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