Molecular analysis of the Escherichia coli phoP-phoQ operon.

Abstract:

:The phoP-phoQ operon of Salmonella typhimurium is a member of the family of two-component regulatory systems and controls expression of the phoN gene that codes for nonspecific acid phosphatase and the genes involved in the pathogenicity of the bacterium. The phoP-phoQ operon of Escherichia coli was cloned on a plasmid vector by complementation of a phoP mutant, and the 4.1-kb nucleotide sequence, which includes the phoP-phoQ operon and its flanking regions, was determined. The phoP-phoQ operon was mapped at 25 min on the standard E. coli linkage map by hybridization with the Kohara mini set library of the E. coli chromosome (Y. Kohara, K. Akiyama, and K. Isono, Cell 50:495-508, 1987). The predicted phoP and phoQ gene products consist of 223 and 486 amino acids with estimated molecular masses of 25,534 and 55,297 Da, respectively, which correspond well with the sizes of the PhoP and PhoQ proteins identified by the maxicell method. The amino acid sequences of PhoP and PhoQ of E. coli were 93 and 86% identical, respectively, to those of S. typhimurium.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Kasahara M,Nakata A,Shinagawa H

doi

10.1128/jb.174.2.492-498.1992

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

492-8

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

174

pub_type

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