Abstract:
:The Rhizobium phaseoli recA gene has been cloned by interspecific complementation of the Fec phenotype of bacteriophage lambda. The cloned gene restored the recombination proficiency and conferred resistance to DNA-damaging agents (methyl methanesulfonate and nitrofurantoin) to an Escherichia coli recA mutant. The direction of transcription and the localization of the recA gene were determined by mutagenesis with phage MudIIPR13 and heterologous hybridization with an E. coli recA probe. An R. phaseoli recA::Spcr mutation was introduced in two R. phaseoli strains by homogenotization. The R. phaseoli recA mutants were more sensitive to DNA-damaging agents and exhibited a 100-fold reduction in recombination frequency as compared with their parental strains. A deletion of the symbiotic plasmid abolishing nodulation was found at high frequency (10(-2)) in R. phaseoli CNF42. This event was recA dependent. In R. phaseoli CFN285, two events of symbiotic instability were found at high frequency (10(-3]: one was a deletion in the symbiotic plasmid, and the other was the loss of whole symbiotic plasmid. In the CFN285 recA::Spcr mutant, only the loss of the symbiotic plasmid was observed.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Martínez-Salazar JM,Romero D,Girard ML,Dávila Gdoi
10.1128/jb.173.10.3035-3040.1991subject
Has Abstractpub_date
1991-05-01 00:00:00pages
3035-40issue
10eissn
0021-9193issn
1098-5530journal_volume
173pub_type
杂志文章abstract::The overlapping fdhA and fdhB genes of Methanobacterium formicicum, which encode the alpha and beta subunits, respectively, of formate dehydrogenase, were cotranscribed as part of an approximately 4.5-kb transcript. An additional gene (fdhC) upstream of fdhA was cotranscribed with fdhA and fdhB. The deduced amino acid...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.15.4997-5004.1992
更新日期:1992-08-01 00:00:00
abstract::The asg mutants of Myxococcus xanthus are defective in production of extracellular A-signal, which serves as a cell density signal for fruiting-body development. The DNA sequence of asgB, one of the three asg genes, was determined. The deduced amino acid sequence of AsgB contains a DNA-binding helix-turn-helix motif n...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.7.2013-2020.1994
更新日期:1994-04-01 00:00:00
abstract::The metabolism of one- and two-carbon compounds by the methylotrophic bacterium Methylobacterium extorquens AM1 involves high carbon flux through the ethylmalonyl coenzyme A (ethylmalonyl-CoA) pathway (EMC pathway). During growth on ethylamine, the EMC pathway operates as a linear pathway carrying the full assimilator...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02478-14
更新日期:2015-02-15 00:00:00
abstract::Lactobacillus casei and Streptococcus faecalis accumulated labeled folic acid and metabolized this compound to poly-gamma-glutamates of chain lengths of up to 11 and 5, respectively. Octa- and nonaglutamates predominated in L. casei, and tetraglutamates predominated in S. faecalis. The most effective monoglutamate sub...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.1.316-325.1983
更新日期:1983-01-01 00:00:00
abstract::Serratia plymuthica strain PRI-2C is a rhizosphere bacterial strain with antagonistic activity against different plant pathogens. Here we present the 5.39-Mb (G+C content, 55.67%) draft genome sequence of S. plymuthica strain PRI-2C with the aim of providing insight into the genomic basis of its antagonistic activity....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00679-12
更新日期:2012-08-01 00:00:00
abstract::A proline analogue, 4,5-dehydro-l-pipecolic acid (baikiain) induces the formation in Salmonella typhimurium of the two enzymes catalyzing the degradation of proline, proline oxidase and Delta(1)-pyrroline-5-carboxylic acid (P5C) dehydrogenase. The level of induction by 20 mm baikiain is about 10% of the maximum level ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.112.3.1134-1141.1972
更新日期:1972-12-01 00:00:00
abstract::Formate dehydrogenase has traditionally been assumed to play an essential role in energy generation during growth on C(1) compounds. However, this assumption has not yet been experimentally tested in methylotrophic bacteria. In this study, a whole-genome analysis approach was used to identify three different formate d...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.1.22-28.2004
更新日期:2004-01-01 00:00:00
abstract::Previously we have shown that flagella and the O-specific polysaccharide of lipopolysaccharide play a role in colonization of the potato root by plant growth-promoting Pseudomonas strains WCS374 and WCS358. In this paper, we describe a novel cell surface-exposed structure in Pseudomonas putida WCS358 examined with a s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.7.1955-1961.1996
更新日期:1996-04-01 00:00:00
abstract::In the nitrogenase system of Klebsiella pneumoniae, nifT is located between nifDK, the structural genes for dinitrogenase, and nifY, whose product is involved in nitrogenase maturation. It is, therefore, a reasonable hypothesis that the NifT protein might also have a role in the maturation of nitrogenase. However, the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.10.2975-2977.1996
更新日期:1996-05-01 00:00:00
abstract::The prevalence of the Moraxella catarrhalis immunoglobulin D (IgD)-binding outer membrane protein MID and its gene was determined in 91 clinical isolates and in 7 culture collection strains. Eighty-four percent of the clinical Moraxella strains expressed MID-dependent IgD binding. The mid gene was detected in all stra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.7.2285-2295.2003
更新日期:2003-04-01 00:00:00
abstract::Upon cold shock, Escherichia coli cell growth transiently stops. During this acclimation phase, specific cold shock proteins (CSPs) are highly induced. At the end of the acclimation phase, their synthesis is reduced to new basal levels, while the non-cold shock protein synthesis is resumed, resulting in cell growth re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.9.2808-2816.2001
更新日期:2001-05-01 00:00:00
abstract::The structural genes of ADPglucose pyrophosphorylase (glgC) and glycogen synthase (glgA) from Salmonella typhimurium LT2 were cloned on a 5.8-kilobase-pair insert in the SalI site of pBR322. A single strand specific radioactive probe containing the N terminus of the Escherichia coli K-12 glgC gene in M13mp8 was used t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.9.4349-4354.1987
更新日期:1987-09-01 00:00:00
abstract::Clusters of cytoplasmic vesicles resembling those in growing hyphal apices of mycelial fungi are found near the tips of buds in three heterobasidiomycetous yeasts, Rhodotorula glutinis, Candida scottii, and Sporobolomyces salmonicolor. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.109.2.922-926.1972
更新日期:1972-02-01 00:00:00
abstract::In this report we present the first description of the isolation and partial characterization of the deoxyribonucleic acid (DNA) polymerase activity from two species of Mycoplasmatales, Mycoplasma orale type 1 and M. hyorhinis. We have identified only a single DNA polymerase species in the mycoplasma crude extracts, a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.132.2.641-649.1977
更新日期:1977-11-01 00:00:00
abstract::The Bacillus subtilis ResD-ResE two-component regulatory system activates genes involved in nitrate respiration in response to oxygen limitation or nitric oxide (NO). The sensor kinase ResE activates the response regulator ResD through phosphorylation, which then binds to the regulatory region of genes involved in ana...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.6.1694-1704.2004
更新日期:2004-03-01 00:00:00
abstract::Sequences of the 16S ribosomal DNA (rDNA) from psychrotolerant and mesophilic strains of the Bacillus cereus group revealed signatures which were specific for these two thermal groups of bacteria. Further analysis of the genomic DNA from a wide range of food and soil isolates showed that B. cereus group strains have b...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.8.2624-2630.1999
更新日期:1999-04-01 00:00:00
abstract::The alpha (62,000-dalton) and beta (49,000-dalton) subunits of Methanosarcina barkeri ATPase were purified to homogeneity. The subunits and ATPase complex were trypsinized in the presence of various nucleotides. ATP and ADP changed the trypsin sensitivity of the alpha subunit in the complex and isolated forms, suggest...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.12.5960-5962.1988
更新日期:1988-12-01 00:00:00
abstract::Using a transcriptional fusion to the lacZ gene, we have analyzed the anaerobic regulation of the hydrogenase 1 (hya) operon in response to different anaerobic growth conditions and to mutations in regulatory genes. We found that the transcription of the hya operon was induced when the growth condition was changed fro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.17.5423-5428.1994
更新日期:1994-09-01 00:00:00
abstract::In Shigella flexneri, the polysaccharide copolymerase (PCP) protein Wzz(SF) confers a modal length of 10 to 17 repeat units (RUs) to the O-antigen (Oag) component of lipopolysaccharide (LPS). PCPs form oligomeric structures believed to be related to their function. To identify functionally important regions within Wzz...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01134-09
更新日期:2010-07-01 00:00:00
abstract::In previous work with membranes of Bacillus subtilis, the succinate dehydrogenase complex was isolated by immunoprecipitation of Triton X-100-solubilized membranes. The complex included a polypeptide with an apparent molecular weight of 19,000, probably attributable to apocytochrome. This paper reports the further cha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.3.933-940.1980
更新日期:1980-12-01 00:00:00
abstract::The Escherichia coli nusA gene, nusAEc, encodes an essential protein that influences transcription elongation. Derivatives of E. coli in which the Salmonella typhimurium nusA gene, nusASt, has replaced nusAEc are viable. Thus, NusASt can substitute for NusAEc in supporting essential bacterial activities. However, hybr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.5.1394-1404.1994
更新日期:1994-03-01 00:00:00
abstract::Falcone, Giuseppe (Rutgers, The State University, New Brunswick, N.J.), and Walter J. Nickerson. Reduction of selenite by intact yeast cells and cell-free preparations. J. Bacteriol. 85:754-762. 1963.-Nonproliferating cell suspensions of Candida albicans rapidly reduced selenite to red, metallic selenium in the absenc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.4.754-762.1963
更新日期:1963-04-01 00:00:00
abstract::Biofilms contribute to virulence of Staphylococcus aureus. Formation of biofilms is multifactorial, involving polysaccharide, protein, and DNA components, which are controlled by various regulators. Here we report that deletion of the rsp gene resulted in an increase in biofilm formation in strain MW2, suggesting that...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05454-11
更新日期:2011-10-01 00:00:00
abstract:UNLABELLED:Bacterial type IV coupling proteins (T4CPs) bind and mediate the delivery of DNA substrates through associated type IV secretion systems (T4SSs). T4CPs consist of a transmembrane domain, a conserved nucleotide-binding domain (NBD), and a sequence-variable helical bundle called the all-alpha domain (AAD). In ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00189-15
更新日期:2015-07-01 00:00:00
abstract::Corynebacterium diphtheriae, a Gram-positive, aerobic bacterium, is the causative agent of diphtheria and cutaneous infections. While mechanisms required for heme iron acquisition are well known in C. diphtheriae, systems involved in the acquisition of other metals such as zinc and manganese remain poorly characterize...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00051-18
更新日期:2018-04-24 00:00:00
abstract::Partial denaturation of Saccharomyces cerevisiae chromosomal DNA was found to occur spontaneously during meiosis. Short regions of strand separation (300 base pairs long) were seen in DNA molecules prepared for electron microscopy by the aqueous spreading technique. These regions were clustered along the DNA. The time...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.2.629-635.1978
更新日期:1978-05-01 00:00:00
abstract::Irradiated L cells infected with Rickettsia tsutsugamushi were studied under the electron microscope to define the morphological growth pattern of the organism. For 2 days after inoculation, no rickettsiae were found either extra- or intracellularly; after 2 days multiple rickettsiae appeared within the host cells wit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.2.879-892.1983
更新日期:1983-05-01 00:00:00
abstract::We have extended our previous studies of the DNA sequences required for growth rate-dependent control of rRNA transcription in Escherichia coli. Utilizing a reporter system suitable for evaluation of promoters with low activities, we have found that the core promoter region of rrnB P1 (-41 to +1 with respect to the tr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.17.5560-5564.1994
更新日期:1994-09-01 00:00:00
abstract::Although four of the five Salmonella pathogenicity islands (SPIs) have been characterized in detail for Salmonella enterica serovar Typhimurium, and the fifth has been characterized for Salmonella enterica serovar Dublin, there have been limited studies to examine them in detail in a range of pathogenic serovars of S....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.12.3624-3635.2003
更新日期:2003-06-01 00:00:00
abstract::A genetic element integrating site specifically into a prokaryotic gene usually carries a copy of the 3' portion of that gene that restores the active gene even as the original is disrupted. A cryptic element in Mesorhizobium loti instead carries a copy of the 5' end of the tRNA gene into which it integrated. This has...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.3.859-860.2002
更新日期:2002-02-01 00:00:00