Abstract:
:We have extended our previous studies of the DNA sequences required for growth rate-dependent control of rRNA transcription in Escherichia coli. Utilizing a reporter system suitable for evaluation of promoters with low activities, we have found that the core promoter region of rrnB P1 (-41 to +1 with respect to the transcription initiation site) is sufficient for growth rate-dependent control of transcription, both in the presence and in the absence of guanosine 3'-diphosphate 5'-diphosphate (ppGpp). The core promoter contains the -10 and -35 hexamers for recognition by the sigma 70 subunit of RNA polymerase but lacks the upstream (UP) element, which increases transcription by interacting with the alpha subunit of RNA polymerase. It also lacks the binding sites for the positive transcription factor FIS. Thus, the UP element, FIS, and ppGpp are not needed for growth rate-dependent regulation of rRNA transcription. In addition, we find that several core promoter mutations, including -10 and -35 hexamer substitutions, severely reduce rrnB P1 activity without affecting growth rate-dependent control. Thus, a high activity is not a determinant of growth rate regulation of rRNA transcription.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Bartlett MS,Gourse RLdoi
10.1128/jb.176.17.5560-5564.1994subject
Has Abstractpub_date
1994-09-01 00:00:00pages
5560-4issue
17eissn
0021-9193issn
1098-5530journal_volume
176pub_type
杂志文章abstract::Earlier observations that phenol is a repellent for Salmonella typhimurium but an attractant for Escherichia coli were confirmed. This behavioral difference was found to correlate with a difference in the effect phenol had on receptor methylation levels; it caused net demethylation in S. typhimurium but net methylatio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.1.371-379.1987
更新日期:1987-01-01 00:00:00
abstract::Although amino acid transport has been extensively studied in bacteria during the past decade, little is known concerning the transport of those amino acids that are biosynthetic intermediates or have multiple fates within the cell. We have studied homoserine and threonine as examples of this phenomenon. Homoserine is...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.117.3.1002-1009.1974
更新日期:1974-03-01 00:00:00
abstract::In the photosynthetic bacterium Rhodobacter capsulatus, the enzyme DNA gyrase has been implicated in the expression of genes for anaerobic metabolic processes such as nitrogen fixation and photosynthesis. To assess the involvement of supercoiling in anaerobic gene expression, we have developed an assay to detect in vi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.9.4836-4843.1989
更新日期:1989-09-01 00:00:00
abstract::The occurrence of the novel regulatory nucleotide bis(3',5')-cyclic diguanylic acid (c-di-GMP) and its relation to cellulose biogenesis in the plant pathogen Agrobacterium tumefaciens was studied. c-di-GMP was detected in acid extracts of 32P-labeled cells grown in various media, and an enzyme responsible for its form...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.12.6649-6655.1989
更新日期:1989-12-01 00:00:00
abstract::Utilizing a homologous gene replacement in order to retain the native promoter and 5' and 3' untranslated messenger regions (and thereby ensure physiological validity), we have shown that deletion of the N-terminal 389 amino acids of the transcriptional activator AREA does not result in nitrogen metabolite derepressio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.21.5762-5764.1998
更新日期:1998-11-01 00:00:00
abstract::The transferred DNA (T-DNA) is transported from Agrobacterium tumefaciens to the nucleus and is stably integrated into the genome of many plant species. It has been proposed that the VirD2 protein, tightly attached to the T-DNA, pilots the T-DNA into the plant cell nucleus and that it is involved in integration. Using...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.3.723-731.1993
更新日期:1993-02-01 00:00:00
abstract::SbmA is an inner membrane protein of Gram-negative bacteria that is involved in the internalization of glycopeptides and prokaryotic and eukaryotic antimicrobial peptides, as well as of peptide nucleic acid (PNA) oligomers. The SbmA homolog BacA is required for the development of Sinorhizobium meliloti bacteroids with...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00818-13
更新日期:2013-12-01 00:00:00
abstract::Mycoplasma ovipneumoniae belongs to Mycoplasma, a genus containing the smallest self-replicating microorganisms, and causes infectious pleuropneumonia in goats and sheep. Nucleotide-binding oligomerization domain-containing protein (NOD2), an intracellular pattern recognition receptor, interacts with muramyl dipeptide...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00689-19
更新日期:2020-09-23 00:00:00
abstract::Plasmid ColIb-P9 of the I alpha incompatibility group is known to encode a DNA primase that acts in the conjugal transfer of the plasmid and can substitute for mutant dnaG gene product in vegetative replication of the Escherichia coli chromosome. The relevant genetic determinant (sog) has previously been cloned into a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.1.1-7.1982
更新日期:1982-07-01 00:00:00
abstract::We have constructed a series of broad-host-range plasmids which use "visual screens" to detect promoter activity. These plasmids contain the pMB1 and pRO1600 origins of replication and are capable of replicating in a wide range of gram-negative bacteria. The genes encoding beta-galactosidase and alkaline phosphatase f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.6.3496-3499.1990
更新日期:1990-06-01 00:00:00
abstract::Shigella spp. are the major cause of bacillary dysentery worldwide. The pathogenic process involves bacterial invasion and lysis of the phagocytic vacuole, followed by replication and movement within the cell cytoplasm and, ultimately, spread directly into adjacent cells. This study demonstrates that S. flexneri cytoc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.4.1229-1237.1999
更新日期:1999-02-01 00:00:00
abstract::The TOL plasmid originally isolated in Pseudomonas putida (arvilla) mt-2 was transmissible to strains of the fluorescens group of Pseudomonas, i.e., P. putida, P. fluorescens, and P. aeruginosa, except for a strain of P. aeruginosa, strain PAO. The same strain, however, could accept the plasmid when its restriction an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.2.527-535.1978
更新日期:1978-02-01 00:00:00
abstract::A novel tungstate and molybdate binding protein has been discovered from the hyperthermophilic archaeon Pyrococcus furiosus. This tungstate transport protein A (WtpA) is part of a new ABC transporter system selective for tungstate and molybdate. WtpA has very low sequence similarity with the earlier-characterized tran...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00548-06
更新日期:2006-09-01 00:00:00
abstract::Nitrate reductase extracted from the membrane of Escherichia coli by alkaline heat treatment was purified to homogeneity and used to prepare specific antibody. Nitrate reductase, precipitated by this antibody from Triton extracts of the membrane, contained a third subunit not present in the purified enzyme used to pre...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.3.1102-1110.1975
更新日期:1975-03-01 00:00:00
abstract::Estrada-Parra, Sergio (Escuela Nacional de Ciencias Biológicas, México, D.F., México), Abel Zamora, and L. F. Bojalil. Immunochemistry of the group-specific polysaccharide of Nocardia brasiliensis. J. Bacteriol. 90:571-574. 1965.-The group-specific polysaccharide of Nocardia brasiliensis was further purified, yielding...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.3.571-574.1965
更新日期:1965-09-01 00:00:00
abstract::Members of Thermococcales harbor a number of genes encoding putative aminotransferase Class III enzymes. Here, we characterized the TK1211 protein from the hyperthermophilic archaeon Thermococcus kodakarensis The TK1211 gene was expressed in T. kodakarensis under the control of the strong, constitutive promoter of the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00617-20
更新日期:2021-01-19 00:00:00
abstract::Vibrio cholerae is the etiologic agent of cholera in humans. Intestinal colonization occurs in a stepwise fashion, initiating with attachment to the small intestinal epithelium. This attachment is followed by expression of the toxin-coregulated pilus, microcolony formation, and cholera toxin (CT) production. We have r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00747-09
更新日期:2009-11-01 00:00:00
abstract::Several authors have suggested that the SOS-associated (sfiA-dependent) system of division inhibition, normally induced by perturbations of DNA replication, also regulates steady-state (unperturbed) cell division. The present work shows that mean cell mass is identical in sfiA+ and sfiA mutant cultures during steady-s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.2.1072-1074.1983
更新日期:1983-02-01 00:00:00
abstract::Escherichia coli possesses three distinct formate dehydrogenase enzymes encoded by the fdnGHI, fdhF, and fdoGHI operons. To examine how two of the formate dehyrogenase operons (fdnGHI and fdhF) are expressed anaerobically in the presence of low, intermediate, and high levels of nitrate, nitrite, and formate, chemostat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.17.5076-5085.2003
更新日期:2003-09-01 00:00:00
abstract::Endoribonuclease RNase E appears to control the rate-limiting step that mediates the degradation of many mRNA species in bacteria. In this work, an RNase E-like activity in Archaea is described. An endoribonucleolytic activity from the extreme halophile Haloarcula marismortui showed the same RNA substrate specificity ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.4.1180-1185.1997
更新日期:1997-02-01 00:00:00
abstract::A new method has been developed in order to select mutants showing decreased puc operon transcription in Rhodobacter sphaeroides 2.4.1. A transcriptional fusion of a promoterless fragment derived from the sacB gene, encoding the levansucrase from Bacillus subtilis, to the upstream regulatory region of the puc operon h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.1.35-45.1996
更新日期:1996-01-01 00:00:00
abstract::Virulence genes in Listeria monocytogenes are coordinately expressed under the control of the transcriptional activator PrfA, encoded by prfA, a member of the cyclic AMP (cAMP) receptor protein (CRP)/FNR family of bacterial regulators. Strain P14-A is a spontaneous mutant of L. monocytogenes serovar 4b which produces ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.5.1533-1540.1997
更新日期:1997-03-01 00:00:00
abstract::Using mixed-species cultures, we have undertaken a study of interactions between two common spore-forming soil bacteria, Bacillus subtilis and Streptomyces coelicolor. Our experiments demonstrate that the development of aerial hyphae and spores by S. coelicolor is inhibited by surfactin, a lipopeptide surfactant produ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00162-06
更新日期:2006-07-01 00:00:00
abstract::The DNAs of strains of three cyanobacterial genera (Anabaena, Plectonema, and Synechococcus) were found to be partially or fully resistant to many restriction endonucleases. This could be due to the absence of specific sequences or to modifications, rendering given sequences resistant to cleavage. The latter explanati...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.4.1934-1939.1988
更新日期:1988-04-01 00:00:00
abstract::Two activites causing nitrite disappearance are found in extracts of Neurospora; one, inducible by nitrate or nitrite and present only in nitrite-utilizing strains, catalyze the stoichiometric reduction of nitrite to ammonia; the other, present in all strains under all conditions, causes the disappearance of nitrite t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.123.2.755-758.1975
更新日期:1975-08-01 00:00:00
abstract::Isolated membrane vesicles from the obligately acidophilic bacterium Bacillus acidocaldarius generated an electrochemical gradient of protons (delta mu- H+) upon energization with ascorbate-phenazine methosulfate at pH 6.0 or 3.0. At pH 6.0, there was little or no transmembrane pH gradient (delta pH), but a transmembr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.2.448-452.1984
更新日期:1984-08-01 00:00:00
abstract::The Yersinia adhesin A (YadA) is a trimeric autotransporter adhesin of enteric yersiniae. It consists of three major domains: a head mediating adherence to host cells, a stalk involved in serum resistance, and an anchor that forms a membrane pore and is responsible for the autotransport function. The anchor contains a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00985-07
更新日期:2007-12-01 00:00:00
abstract::Unlike the great majority of the aerobactin-producing enteric bacteria documented in the literature, Enterobacter cloacae EK33, isolated from a case of human neonatal meningitis, did not show any homology at the DNA level with the prototype aerobactin system encoded by the ColV-K30 plasmid. However, both the nuclear m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.12.5539-5544.1988
更新日期:1988-12-01 00:00:00
abstract::Variations of the membrane filter technique for deoxyribonucleic acid (DNA) hybridizations were studied with respect to Streptomyces species. At the temperatures required for specific hybridization of DNA with the high melting temperature (T(m)) characteristic of Streptomyces, large amounts (up to 97%) of filter-bound...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.3.1086-1094.1970
更新日期:1970-12-01 00:00:00
abstract::Methanogenesis from the non-physiological C1 donors thioproline, thiazolidine, hexamethylenetetramine, formaldehyde (HCHO), and HOCH2-S-coenzyme M (CoM) was catalyzed by cell extracts of Methanobacterium thermoautotrophicum under a hydrogen atmosphere. Tetrahydromethanopterin (H4MPT) and HS-CoM were required in the re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.161.2.696-701.1985
更新日期:1985-02-01 00:00:00