Methods for the determination of deoxyribonucleic Acid homologies in streptomyces.

Abstract:

:Variations of the membrane filter technique for deoxyribonucleic acid (DNA) hybridizations were studied with respect to Streptomyces species. At the temperatures required for specific hybridization of DNA with the high melting temperature (T(m)) characteristic of Streptomyces, large amounts (up to 97%) of filter-bound DNA became eluted, in all reaction mixtures studied, within 21 hr. In most solutions this leaching was increased by the presence of sheared denatured DNA. Incubation of DNA-loaded filters in a solution of 50% formamide containing 6x standard saline citrate, at 48 C for 40 hr, was judged to be the best set of conditions tested based on relatively good retention of immobilized DNA, very low hybridization with unrelated DNA of a similarly high T(m) (from Sarcina lutea), and the formation of complexes similar in thermal stability to the native DNA. The expression of results as sheared DNA bound in relation to long-chain DNA retained is recommended when a high concentration of sheared DNA relative to immobilized DNA is used.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Okanishi M,Gregory KF

doi

10.1128/JB.104.3.1086-1094.1970

keywords:

subject

Has Abstract

pub_date

1970-12-01 00:00:00

pages

1086-94

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

104

pub_type

杂志文章
  • Metabolism of dibutylphthalate and phthalate by Micrococcus sp. strain 12B.

    abstract::Micrococcus sp. strain 12B was isolated by enriching for growth with dibutylphthalate as the sole carbon and energy source. A pathway for the metabolism of dibutylphthalate and phthalate by micrococcus sp. strain 12B is proposed: dibutylphthalate leads to monobutylphthalate leads to phthalate leads to 3,4-dihydro-3,4-...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.151.1.48-57.1982

    authors: Eaton RW,Ribbons DW

    更新日期:1982-07-01 00:00:00

  • PilMNOPQ from the Pseudomonas aeruginosa type IV pilus system form a transenvelope protein interaction network that interacts with PilA.

    abstract::Pseudomonas aeruginosa type IV pili (T4P) are virulence factors that promote infection of cystic fibrosis and immunosuppressed patients. As the absence of T4P impairs colonization, they are attractive targets for the development of novel therapeutics. Genes in the pilMNOPQ operon are important for both T4P assembly an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00032-13

    authors: Tammam S,Sampaleanu LM,Koo J,Manoharan K,Daubaras M,Burrows LL,Howell PL

    更新日期:2013-05-01 00:00:00

  • Detergent-resistant Streptococcus faecium derivatives that display conditional penicillin lysis.

    abstract::Three spontaneous derivatives of Streptococcus faecium ATCC 9790, originally isolated as conditionally Triton X-100 detergent-resistant at 25 degrees C, displayed normal penicillin-induced rates of lysis at 37 degrees C but substantially reduced rates of lysis and killing at 25 degrees C. The addition of exogenous uns...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.159.2.805-807.1984

    authors: Pucci MJ,Daneo-Moore L

    更新日期:1984-08-01 00:00:00

  • Effects of environmental changes on expression of the oligopeptide permease (opp) genes of Borrelia burgdorferi.

    abstract::We analyzed expression of a putative oligopeptide permease (Opp) of Borrelia burgdorferi. Unlike the opp operons of other bacteria for which there is a single substrate binding protein, B. burgdorferi codes for three substrate binding proteins (OppA-I to -III) in its opp operon and an additional two homologs on plasmi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.22.6198-6206.2002

    authors: Wang XG,Lin B,Kidder JM,Telford S,Hu LT

    更新日期:2002-11-01 00:00:00

  • Methylation Warfare: Interaction of Pneumococcal Bacteriophages with Their Host.

    abstract::Virus-host interactions are regulated by complex coevolutionary dynamics. In Streptococcus pneumoniae, phase-variable type I restriction-modification (R-M) systems are part of the core genome. We hypothesized that the ability of the R-M systems to switch between six target DNA specificities also has a key role in prev...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00370-19

    authors: Furi L,Crawford LA,Rangel-Pineros G,Manso AS,De Ste Croix M,Haigh RD,Kwun MJ,Engelsen Fjelland K,Gilfillan GD,Bentley SD,Croucher NJ,Clokie MR,Oggioni MR

    更新日期:2019-09-06 00:00:00

  • Streptococcus faecium mutants that are temperature sensitive for cell growth and show alterations in penicillin-binding proteins.

    abstract::The penicillin-binding proteins (PBPs) of 209 cell division (or growth) temperature-sensitive mutants of Streptococcus faecium were analyzed in this study. A total of nine strains showed either constitutive or temperature-sensitive conditional damage in the PBPs. Analysis of these nine strains yielded the following re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.6.2432-2439.1987

    authors: Canepari P,Lleò MM,Fontana R,Satta G

    更新日期:1987-06-01 00:00:00

  • Effects of selected inhibitors on electron transport in Neisseria gonorrhoeae.

    abstract::The electron transport system of Neisseria gonorrhoeae was partially characterized by using spectrophotometric, spectroscopic, and oxygen consumption measurements. The effects of selected electron transport inhibitors (amytal, rotenone, 2-heptyl-4-hydroxyquinoline, antimycin A1, and potassium cyanide [KCN]) on electro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.134.2.537-545.1978

    authors: Kenimer EA,Lapp DF

    更新日期:1978-05-01 00:00:00

  • HexR Controls Glucose-Responsive Genes and Central Carbon Metabolism in Neisseria meningitidis.

    abstract:UNLABELLED:Neisseria meningitidis, an exclusively human pathogen and the leading cause of bacterial meningitis, must adapt to different host niches during human infection. N. meningitidis can utilize a restricted range of carbon sources, including lactate, glucose, and pyruvate, whose concentrations vary in host niches...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00659-15

    authors: Antunes A,Golfieri G,Ferlicca F,Giuliani MM,Scarlato V,Delany I

    更新日期:2015-12-07 00:00:00

  • Structural and biochemical analysis of the sheath of Phormidium uncinatum.

    abstract::The sheath of the filamentous, gliding cyanobacterium Phormidium uncinatum was studied by using light and electron microscopy. In thin sections and freeze fractures the sheath was found to be composed of helically arranged carbohydrate fibrils, 4 to 7 nm in diameter, which showed a substantial degree of crystallinity....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.15.3923-3932.1998

    authors: Hoiczyk E

    更新日期:1998-08-01 00:00:00

  • Alteration of tryptophan-mediated regulation in Neurospora crassa by indoleglycerol phosphate.

    abstract::The accumulation of imidazoleglycerol phosphate during growth of Neurospora crassa in the presence of 3-amino-1,2,4-triazole was found to cause derepression of tryptophan synthetase and to inhibit the induction of kynureninase. Accumulation of indoleglycerol phosphate in response to growth in the presence of indole ac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.5.1608-1614.1968

    authors: Turner JR,Matchett WH

    更新日期:1968-05-01 00:00:00

  • Genetic rearrangement associated with in vivo mucoid conversion of Pseudomonas aeruginosa PAO is due to insertion elements.

    abstract::The conversion of Pseudomonas aeruginosa PAO to the mucoid phenotype has been reported for a chronic pulmonary infection model in rats (D. E. Woods, P. A. Sokol, L. E. Bryan, D. G. Storey, S. J. Mattingly, H. J. Vogel, and H. Ceri, J. Infect. Dis. 163:143-149, 1991). This conversion was associated with a genetic rearr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.3.553-562.1994

    authors: Sokol PA,Luan MZ,Storey DG,Thirukkumaran P

    更新日期:1994-02-01 00:00:00

  • Mutants of Escherichia coli with a growth requirement for either lysine or pyridoxine.

    abstract::Two distinct phenotypic classes of lysine requiring auxotrophs of Escherichia coli are described. Mutants of the LysA class produce little or no active diaminopimelic acid (DAP) decarboxylase and specifically require lysine for growth. Mutants of the LysB class produce a cryptic DAP decarboxylase which can be activate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.3.988-998.1971

    authors: Bukhari AI,Taylor AL

    更新日期:1971-03-01 00:00:00

  • High- and low-threshold genes in the Spo0A regulon of Bacillus subtilis.

    abstract::The master regulator for entry into sporulation in Bacillus subtilis is the response regulator Spo0A, which directly governs the expression of about 121 genes. Using cells in which the synthesis of Spo0A was under the control of an inducible promoter or in which production of the regulatory protein was impaired by a p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.4.1357-1368.2005

    authors: Fujita M,González-Pastor JE,Losick R

    更新日期:2005-02-01 00:00:00

  • Kasugamycin-resistant mutants of Bacillus subtilis.

    abstract::Kasugamycin-resistant mutants of Bacillus subtilis were isolated and classified into two groups, one of which had resistance to kasugamycin in in vitro protein synthesis and mapped in the ribosomal region. The other group had no resistance to kasugamycin in in vitro protein synthesis and had weak cross-resistance to g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.135.3.1149-1150.1978

    authors: Tominaga A,Kobayashi Y

    更新日期:1978-09-01 00:00:00

  • Chemotaxis arrays in Vibrio species and their intracellular positioning by the ParC/ParP system.

    abstract::Most motile bacteria are able to bias their movement towards more favorable environments or to escape from obnoxious substances by a process called chemotaxis. Chemotaxis depends on a chemosensory system that is able to sense specific environmental signals and generate a behavioral response. Typically, the signal is t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00793-17

    authors: Ringgaard S,Yang W,Alvarado A,Schirner K,Briegel A

    更新日期:2018-03-12 00:00:00

  • Genetic selection and DNA sequences of 4.5S RNA homologs.

    abstract::A general strategy for cloning the functional homologs of an Escherichia coli gene was used to clone homologs of 4.5S RNA from other bacteria. The genes encoding these homologs were selected by their ability to complement a deletion of the gene for 4.5S RNA. DNA sequences of the regions encoding the homologs were dete...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.12.6517-6520.1989

    authors: Brown S,Thon G,Tolentino E

    更新日期:1989-12-01 00:00:00

  • The sufR gene (sll0088 in Synechocystis sp. strain PCC 6803) functions as a repressor of the sufBCDS operon in iron-sulfur cluster biogenesis in cyanobacteria.

    abstract::The suf operon is composed of four genes (sufB, sufC, sufD, and sufS) and is highly conserved in the genomes of cyanobacteria. Open reading frame sll0088 in Synechocystis sp. strain PCC 6803 is located near the 5' end of the suf operon but is transcribed in the direction opposite that of the suf operon. We previously ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.4.956-967.2004

    authors: Wang T,Shen G,Balasubramanian R,McIntosh L,Bryant DA,Golbeck JH

    更新日期:2004-02-01 00:00:00

  • Cloning and characterization of the sigA gene encoding the major sigma subunit of Rhizobium meliloti.

    abstract::Using PCR to create a probe based on conserved region 2 of sigma factors, we have cloned the sigA gene coding for the major sigma factor of Rhizobium meliloti. The 684-residue protein encoded by the sigA gene was expressed in vitro in coupled transcription-translation experiments with R. meliloti extracts and migrated...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.23.6952-6957.1995

    authors: Rushing BG,Long SR

    更新日期:1995-12-01 00:00:00

  • Surface protein composition of Aeromonas hydrophila strains virulent for fish: identification of a surface array protein.

    abstract::The surface protein composition of members of a serogroup of Aeromonas hydrophila which exhibit high virulence for fish was examined. Treatment of whole cells of representative strain A. hydrophila TF7 with 0.2 M glycine buffer (pH 4.0) resulted in the release of sheets of a tetragonal surface protein array. Sodium do...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.2.499-506.1988

    authors: Dooley JS,Trust TJ

    更新日期:1988-02-01 00:00:00

  • Chemical analyses of asporogenic mutants of Bacillus cereus.

    abstract::Lundgren, D. G. (Syracuse University, Syracuse, N.Y.) and J. J. Cooney. Chemical analyses of asporogenic mutants of Bacillus cereus. J. Bacteriol. 83:1287-1293. 1962.-Bacillus cereus ATCC 4342 and three temperature-sensitive asporogenic mutants were compared regarding some basic cellular components and response to met...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.6.1287-1293.1962

    authors: LUNDGREN DG,COONEY JJ

    更新日期:1962-06-01 00:00:00

  • Analysis of ThiC variants in the context of the metabolic network of Salmonella enterica.

    abstract::In bacteria, the 4-amino-hydroxymethyl-2-methylpyrimidine (HMP) moiety of thiamine is synthesized from 5-aminoimidazole ribotide (AIR), a branch point metabolite of purine and thiamine biosynthesis. ThiC is a member of the radical S-adenosylmethionine (AdoMet) superfamily and catalyzes the complex chemical rearrangeme...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01361-12

    authors: Palmer LD,Dougherty MJ,Downs DM

    更新日期:2012-11-01 00:00:00

  • Reduce, Induce, Thrive: Bacterial Redox Sensing during Pathogenesis.

    abstract::The abundance of oxidants and reductants must be balanced for an organism to thrive. Bacteria have evolved methods to prevent redox imbalances and to mitigate their deleterious consequences through the expression of detoxification enzymes, antioxidants, and systems to repair or degrade damaged proteins and DNA. Regula...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审

    doi:10.1128/JB.00128-18

    authors: Reniere ML

    更新日期:2018-08-10 00:00:00

  • EVIDENCE OF LYSOGENY IN DERIVATIVES OF ESCHERICHIA COLI.

    abstract::Frampton, E. W. (The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston), and B. R. Brinkley. Evidence of lysogeny in derivatives of Escherichia coli. J. Bacteriol. 90:446-452. 1965.-Exposure of cultures of several thymine-requiring derivatives of Escherichia coli strain 15 to radiation [X rays o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.2.446-452.1965

    authors: FRAMPTON EW,BRINKLEY BR

    更新日期:1965-08-01 00:00:00

  • Interaction of native and mutant MecI repressors with sequences that regulate mecA, the gene encoding penicillin binding protein 2a in methicillin-resistant staphylococci.

    abstract::Methicillin resistance in staphylococci is mediated by PBP2a, a penicillin binding protein with low affinity for beta-lactam antibiotics. The gene encoding PBP2a, mecA, is transcriptionally regulated in some clinical isolates by mecR1 and mecI, genes divergently transcribed from mecA that encode a signal transducer an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.8.2160-2166.1998

    authors: Sharma VK,Hackbarth CJ,Dickinson TM,Archer GL

    更新日期:1998-04-01 00:00:00

  • Identification, cloning, and characterization of the Ima operon, whose gene products are unique to Listeria monocytogenes.

    abstract::The lmaA gene of Listeria monocytogenes encodes a protein capable of inducing delayed-type hypersensitivity reactions in L. monocytogenes-immune mice (S. Göhmann, M. Leimeister-Wachter, E. Schiltz, W. Goebel, and T. Chakraborty, M. Microbiol. 4:1091-1099, 1990). Here we show that it is the last gene of the lma operon,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.8.2707-2716.1997

    authors: Schäferkordt S,Chakraborty T

    更新日期:1997-04-01 00:00:00

  • Coupled changes in translation and transcription during cobalamin-dependent regulation of btuB expression in Escherichia coli.

    abstract::The level of the vitamin B12 transport protein BtuB in the outer membrane of Escherichia coli is strongly reduced by growth in the presence of cobalamins. Previous analyses of regulatory mutants and of btuB-lacZ fusions indicated that the primary site of btuB gene regulation was at the translational level, and this re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.24.6719-6728.1998

    authors: Nou X,Kadner RJ

    更新日期:1998-12-01 00:00:00

  • Amplification of DNA at a prophage attachment site in Haemophilus influenzae.

    abstract::The Escherichia coli plasmids pBR322 and pBR327 can be taken up by Haemophilus influenzae but do not replicate in this organism; however, integration of pBR into the H. influenzae chromosome was achieved by ligation to a fragment of the Haemophilus phage S2 that carried a phage attachment site (attP). Once these seque...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.4.1898-1903.1989

    authors: Kauc L,Goodgal SH

    更新日期:1989-04-01 00:00:00

  • Nitric oxide, nitrite, and Fnr regulation of hmp (flavohemoglobin) gene expression in Escherichia coli K-12.

    abstract::Escherichia coli possesses a soluble flavohemoglobin, with an unknown function, encoded by the hmp gene. A monolysogen containing an hmp-lacZ operon fusion was constructed to determine how the hmp promoter is regulated in response to heme ligands (O2, NO) or the presence of anaerobically utilized electron acceptors (n...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.18.5487-5492.1996

    authors: Poole RK,Anjum MF,Membrillo-Hernández J,Kim SO,Hughes MN,Stewart V

    更新日期:1996-09-01 00:00:00

  • A homolog of the CtrA cell cycle regulator is present and essential in Sinorhizobium meliloti.

    abstract::During development of the symbiotic soil bacterium Sinorhizobium meliloti into nitrogen-fixing bacteroids, DNA replication and cell division cease and the cells undergo profound metabolic and morphological changes. Regulatory genes controlling the early stages of this process have not been identified. As a first step ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.10.3204-3210.2001

    authors: Barnett MJ,Hung DY,Reisenauer A,Shapiro L,Long SR

    更新日期:2001-05-01 00:00:00

  • Physiological effects of anti-TRAP protein activity and tRNA(Trp) charging on trp operon expression in Bacillus subtilis.

    abstract::The Bacillus subtilis anti-TRAP protein regulates the ability of the tryptophan-activated TRAP protein to bind to trp operon leader RNA and promote transcription termination. AT synthesis is regulated both transcriptionally and translationally by uncharged tRNA(Trp). In this study, we examined the roles of AT synthesi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01820-07

    authors: Cruz-Vera LR,Gong M,Yanofsky C

    更新日期:2008-03-01 00:00:00