Kasugamycin-resistant mutants of Bacillus subtilis.

abstract：:The kil-kor regulon of the self-transmissible, broad-host-range plasmid RK2 is a unique network with eight coregulated operons. Among the genes encoded by the kil-kor regulon are trfA, which encodes the replication initiator, and several kil loci (kilA, kilB, kilC, and kilE), each of which is lethal to the host cell i...

journal_title：Journal of bacteriology

authors： Thomson VJ,Jovanovic OS,Pohlman RF,Chang CH,Figurski DH

更新日期：1993-04-01 00:00:00

abstract：:Acinetobacter calcoaceticus BD413 produces an extracellular lipase, which is encoded by the lipA gene. Five lipase-deficient mutants have been generated via random insertion mutagenesis. Phenotypic characterization of these mutants revealed the presence of as many as four lipolytic enzymes in A. calcoaceticus. Biochem...

journal_title：Journal of bacteriology

authors： Kok RG,van Thor JJ,Nugteren-Roodzant IM,Vosman B,Hellingwerf KJ

更新日期：1995-06-01 00:00:00

abstract：:Protein phosphorylation was demonstrated in Bradyrhizobium japonicum bacteroids in vivo and in cultures in vivo and in vitro. Comparison of in vivo-labeled phosphoproteins of bacteroids and of cultured cells showed differences in both the pattern and intensity of labeling. In cultured cells, comparison of the labeling...

journal_title：Journal of bacteriology

authors： Karr DB,Emerich DW

更新日期：1989-06-01 00:00:00

abstract：:Transcription of the dnaA gene of the cyanobacterium Synechocystis sp. strain PCC 6803 is light dependent and yields a monocistronic mRNA, as determined by Northern analysis. Surprisingly, mutants with inactivated dnaA were viable. In batch cultures under standard conditions, the mutants grew like the wild type and di...

journal_title：Journal of bacteriology

authors： Richter S,Hagemann M,Messer W

更新日期：1998-09-01 00:00:00

abstract：:We have isolated a hybrid plasmid, pDB(mei2)2, containing a 7.4-kilobases (kb) DNA fragment from a Schizosaccharomyces pombe genomic library which is able to complement the mei2 mutation of S. pombe. Integration of the cloned DNA sequence at the mei2 site on chromosome I demonstrated that it contained the mei2 gene. T...

journal_title：Journal of bacteriology

authors： Shimoda C,Uehira M,Kishida M,Fujioka H,Iino Y,Watanabe Y,Yamamoto M

更新日期：1987-01-01 00:00:00

abstract：:We have cloned the Micromonospora viridifaciens neuraminidase (EC 3.2.1.18) gene (nedA) in Streptomyces lividans. This was accomplished by using the vector pIJ702 and BglII-BclI libraries of M. viridifaciens chromosomal inserts created in S. lividans. The libraries were screened for the expression of neuraminidase by ...

journal_title：Journal of bacteriology

authors： Sakurada K,Ohta T,Hasegawa M

更新日期：1992-11-01 00:00:00

abstract：:Herman, Alberta (University of Wisconsin, Madison) and Harlyn Halvorson. Identification of the structural gene for beta-glucosidase in Saccharomyces lactis. J. Bacteriol. 85:895-900. 1963.-Three allelic forms (B(h, m, l)) of the structural gene for beta-glucosidase have been identified in the yeast Saccharomyces lacti...

journal_title：Journal of bacteriology

authors： HERMAN A,HALVORSON H

更新日期：1963-04-01 00:00:00

abstract：:The tyrT (tRNA(1TYr)) locus of Escherichia coli B differs structurally from that of K-12 strains by the absence of 2 of 3.14 terminal repeat sequences. ...

journal_title：Journal of bacteriology

authors： Timms AR,Bridges BA

更新日期：1996-04-01 00:00:00

abstract：:Pleiotropic effects of two ribosomal mutations for cycloheximide resistance were studied in a double-resistant homocaryon. The results obtained indicated that the combination of the two ribosomal mutations results in: (i) morphological abnormalities which suggest a severe distortion in the extension of cell walls and ...

journal_title：Journal of bacteriology

authors： Vomvoyanni VE,Argyrakis MP

更新日期：1979-08-01 00:00:00

abstract：:A split UV light dose procedure was used in Escherichia coli to induce an SOS function, RecA protein amplification, which was measured by an immunoradiometric assay. The SOS system was partially induced after the first UV irradiation, and the inducing effects of subsequent identical UV doses were quantified. Variation...

journal_title：Journal of bacteriology

authors： Calsou P,Salles B

更新日期：1985-06-01 00:00:00

abstract：:The predominant pool of organic matter on earth is derived from the biological reduction and assimilation of carbon dioxide gas, catalyzed primarily by the enzyme ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO). By virtue of its capacity to use molecular oxygen as an alternative and competing gaseous substra...

journal_title：Journal of bacteriology

authors： Watson GM,Yu JP,Tabita FR

更新日期：1999-03-01 00:00:00

abstract：:The R factor NR1 consists of two components: a resistance transfer factor which harbors the tetracycline resistance genes (RTF-TC) and the r-determinants component which harbors the other drug resistance genes. Using partial denaturation mapping it is possible to distinguish the RTF-TC region from the r-determinants r...

journal_title：Journal of bacteriology

authors： Perlman D,Twose TM,Holland MJ,Rownd RH

更新日期：1975-09-01 00:00:00

abstract：:The activity of the ATP:GTP 3'-pyrophosphotransferase (guanosine pentaphosphate synthetase I [GPSI]) from Streptomyces antibioticus is stimulated maximally by methanol at 20% (vol/vol) in assay mixtures. Although the enzyme is not activated by ribosomes, its activity is stimulated by tRNA (uncharged or charged) and by...

journal_title：Journal of bacteriology

authors： Jones GH

更新日期：1994-03-01 00:00:00

abstract：:Antigen 43 has been identified as a unique protein complex in the outer membrane of Escherichia coli. The complex contains two different polypeptides, alpha (Mr, 60,000) and beta (Mr, 53,000), in equal stoichiometry (P. Owen, P. Caffrey, and L.-G. Josefsson, J. Bacteriol. 169:3770-3777, 1987). The alpha subunit was re...

journal_title：Journal of bacteriology

authors： Caffrey P,Owen P

更新日期：1989-07-01 00:00:00

abstract：:Chlamydia spp. exhibit a unique biphasic developmental cycle whereby infectious elementary bodies (EBs) invade host epithelial cells and differentiate into noninfectious, metabolically active reticulate bodies (RBs). EBs posses a unique outer envelope where rigidity is achieved by disulfide bonding among cysteine-rich...

journal_title：Journal of bacteriology

authors： Betts-Hampikian HJ,Fields KA

更新日期：2011-12-01 00:00:00

abstract：:The steps in the biosynthetic transformation of GTP to 7,8-dihydro-D-erythro-neopterin (H2neopterin), the precursor to the modified folates found in the methanogenic archaea, has been elucidated for the first time in two members of the domain Archaea. In Methanococcus thermophila and Methanobacterium thermoautotrophic...

journal_title：Journal of bacteriology

authors： Howell DM,White RH

更新日期：1997-08-01 00:00:00

abstract：:The glutamate permeation system in Escherichia coli K-12 consists of three genes: gltC, gltS, and gltR. The genes gltC and gltS are very closely linked, and are located between the pyrE and tna loci, in the following order: tna, gltC, gltS, pyrE; gltR is located near the metA gene. The three glt genes constitute a reg...

journal_title：Journal of bacteriology

authors： Marcus M,Halpern YS

更新日期：1969-03-01 00:00:00

abstract：:Through use of multiply disomic strains, the genes arg1 and arg8 were excluded from all of chromosomes I to XVII except (i) XV and (ii) IX and XV, respectively. Further aneuploid analyses showed that these two genes were on the same chromosome. By tetrad analysis, arg1 was shown to be linked to SUP3 on the left arm of...

journal_title：Journal of bacteriology

authors： Hilger F,Mortimer RK

更新日期：1980-01-01 00:00:00

abstract：:A mutant of Escherichia coli has been isolated, the growth of which is inhibited by low concentrations (1 mm) of NaCl. High levels of magnesium, calcium, or strontium in the medium permit growth in the presence of sodium. The metal content of the inhibited mutant is normal, but the strain is unable to tolerate levels ...

journal_title：Journal of bacteriology

authors： Lusk JE,Kennedy EP

更新日期：1972-03-01 00:00:00

abstract：:Glycogen accumulation in Escherichia coli is inversely related to the growth rate and occurs most actively when cells enter the stationary phase. The levels of the three biosynthetic enzymes undergo corresponding changes under these conditions, suggesting that genetic control of enzyme biosynthesis may account for at ...

journal_title：Journal of bacteriology

authors： Romeo T,Preiss J

更新日期：1989-05-01 00:00:00

abstract：:The Sinorhizobium meliloti megaplasmid pSymA has previously been implicated in gluconate utilization. We report a locus on pSymA encoding a putative tripartite ATP-independent periplasmic (TRAP) transporter that is required for gluconate utilization. The expression of this locus is negatively regulated by a GntR famil...

journal_title：Journal of bacteriology

authors： Steele TT,Fowler CW,Griffitts JS

更新日期：2009-02-01 00:00:00

abstract：:The genetic organization of the polypeptides required for the biosynthesis of the F41 adhesin of enterotoxigenic Escherichia coli strains was investigated. Maxicell analysis demonstrated that a recombinant plasmid which mediated mannose-resistant hemagglutination and F41 antigen production encoded four polypeptides of...

journal_title：Journal of bacteriology

authors： Anderson DG,Moseley SL

更新日期：1988-10-01 00:00:00

abstract：:A temperature-sensitive Schizosaccharomyces pombe mutant, cdc16-116, has been isolated which undergoes uncontrolled septation during its cell division cycle. The mutant accumulates two types of cells after 3 h of growth at the restrictive temperature: (i) type I cells (85% of the population), which complete nuclear di...

journal_title：Journal of bacteriology

authors： Minet M,Nurse P,Thuriaux P,Mitchison JM

更新日期：1979-01-01 00:00:00

abstract：:A catabolite-sensitive promoter was found to be involved in transcription of the heat shock regulatory gene rpoH encoding the sigma 32 protein. Expression of lacZ from the operon fusion, rpoHp-lacZ, was partially inhibited by glucose added to the broth medium. Dissection of the rpoH promoter region allowed us to local...

journal_title：Journal of bacteriology

authors： Nagai H,Yano R,Erickson JW,Yura T

更新日期：1990-05-01 00:00:00

abstract：:A reaction center H- strain (RCH-) of Rhodobacter sphaeroides, PUHA1, was made by in vitro deletion of an XhoI restriction endonuclease fragment from the puhA gene coupled with insertion of a kanamycin resistance gene cartridge. The resulting construct was delivered to R. sphaeroides wild-type 2.4.1, with the defectiv...

journal_title：Journal of bacteriology

authors： Sockett RE,Donohue TJ,Varga AR,Kaplan S

更新日期：1989-01-01 00:00:00

abstract：:During the transition from a free-swimming, single-cell lifestyle to a sessile, multicellular state called a biofilm, bacteria produce and secrete an extracellular matrix comprised of nucleic acids, exopolysaccharides, and adhesion proteins. The Vibrio cholerae biofilm matrix contains three major protein components, R...

journal_title：Journal of bacteriology

authors： Giglio KM,Fong JC,Yildiz FH,Sondermann H

更新日期：2013-07-01 00:00:00

abstract：:Nosocomial infections caused by antibiotic-resistant Klebsiella pneumoniae are emerging as a major health problem worldwide, while community-acquired K. pneumoniae infections present with a range of diverse clinical pictures in different geographic areas. In particular, an invasive form of K. pneumoniae that causes li...

journal_title：Journal of bacteriology

authors： Wu KM,Li LH,Yan JJ,Tsao N,Liao TL,Tsai HC,Fung CP,Chen HJ,Liu YM,Wang JT,Fang CT,Chang SC,Shu HY,Liu TT,Chen YT,Shiau YR,Lauderdale TL,Su IJ,Kirby R,Tsai SF

更新日期：2009-07-01 00:00:00

abstract：:Plasma cells obtained from the peripheral blood of a patient with multiple myeloma was incubated in serum and Krebs-Ringer bicarbonate buffer with (14)C-labeled glucose, acetate, and propionate. Glucose utilization by these cells amounted to 0.5 mumole per hr per 10(8) cells and was mainly via the Embden-Meyerhof path...

journal_title：Journal of bacteriology

authors： Stjernholm RL

更新日期：1967-05-01 00:00:00

abstract：:The CbpA protein is an analog of the DnaJ molecular chaperone of Escherichia coli. To gain insight into the function of CbpA, we examined the nature of a cbpA null mutation with special reference to those of dnaK and dnaJ null mutations. In particular, the cbpA dnaJ double-null mutant was found to exhibit severe defec...

journal_title：Journal of bacteriology

authors： Ueguchi C,Shiozawa T,Kakeda M,Yamada H,Mizuno T

更新日期：1995-07-01 00:00:00

abstract：:Yeast mutants deficient in orotidine-5'-phosphate (OMP) pyrophosphorylase activity have been obtained. The gene encoding this enzyme is unlinked to the four pyrimidine genes previously described. The specific activity of OMP pyrophosphorylase remains constant under all of the physiological conditions used to repress, ...

journal_title：Journal of bacteriology

authors： Jund R,Lacroute F

更新日期：1972-01-01 00:00:00