Regulation of orotidylic acid pyrophosphorylase in Saccharomyces cerevisiae.

abstract：:Two key elements that are thought to be required for replication initiation in eubacteria are the DnaA protein, a trans-acting factor, and the replication origin, a cis-acting element. As a first step in studying the replication initiation process in mycobacteria, we have isolated a 4-kb chromosomal DNA fragment from ...

journal_title：Journal of bacteriology

authors： Rajagopalan M,Qin MH,Nash DR,Madiraju MV

更新日期：1995-11-01 00:00:00

abstract：:The crystal structure of the Na(+)-coupled melibiose permease of Salmonella enterica serovar Typhimurium (MelBSt) demonstrates that MelB is a member of the major facilitator superfamily of transporters. Arg residues at positions 295, 141, and 363 are involved in interdomain interactions at the cytoplasmic side by gove...

journal_title：Journal of bacteriology

authors： Amin A,Ethayathulla AS,Guan L

更新日期：2014-09-01 00:00:00

abstract：:Mutants of the fhuA gene of Escherichia coli K-12, which encodes a receptor protein in the outer membrane, took up ferrichrome after exposure to pronase, whereas fhuB mutants remained transport negative. The latter finding supports our previous proposal that fhuB mutants are defective in a function that residues in th...

journal_title：Journal of bacteriology

authors： Wookey PJ,Hussein S,Braun V

更新日期：1981-06-01 00:00:00

abstract：:We have isolated the gene encoding L-allo-threonine aldolase (L-allo-TA) from Aeromonas jandaei DK-39, a pyridoxal 5'-phosphate (PLP)-dependent enzyme that stereospecifically catalyzes the interconversion of L-allo-threonine and glycine. The gene contains an open reading frame consisting of 1,014 nucleotides correspon...

journal_title：Journal of bacteriology

authors： Liu JQ,Dairi T,Kataoka M,Shimizu S,Yamada H

更新日期：1997-06-01 00:00:00

abstract：:A Pediococcus cerevisiae mutant that actively accumulated folate (PteGlu), in contrast to the wild-type, was also found to exhibit changes in the pattern of uptake of 5-methyl-tetrahydrofolate (5-CH3-H4PteGlu) and amethopterin. Most of the 5-CH3-H4PteGlue accumulated through a glucose- and temperature-dependent proces...

journal_title：Journal of bacteriology

authors： Mandelbaum-Shavit F,Grossowicz N

更新日期：1975-08-01 00:00:00

abstract：:The phs mutation, which causes a pleiotropic growth defect, has been mapped and shown to be an allele of rpoA, the gene for the alpha subunit of RNA polymerase. The mutation is shown to cause a transcription defect in the arabinose operon, araBAD. ...

journal_title：Journal of bacteriology

authors： Rowland GC,Giffard PM,Booth IR

更新日期：1985-11-01 00:00:00

abstract：:In Salmonella enterica serovar Typhimurium, the stationary-phase sigma factor sigma(S) (RpoS) is required for virulence, stress resistance, biofilm formation, and development of the rdar morphotype. This morphotype is a multicellular behavior characterized by expression of the adhesive extracellular matrix components ...

journal_title：Journal of bacteriology

authors： Robbe-Saule V,Jaumouillé V,Prévost MC,Guadagnini S,Talhouarne C,Mathout H,Kolb A,Norel F

更新日期：2006-06-01 00:00:00

abstract：:The stationary phase/general stress response sigma factor RpoS (σ(S)) is necessary for adaptation and restoration of homeostasis in stationary phase. As a physiological consequence, its levels are tightly regulated at least at two levels. Multiple small regulatory RNA molecules modulate its translation, in a manner th...

journal_title：Journal of bacteriology

authors： Thompson KM,Gottesman S

更新日期：2014-02-01 00:00:00

abstract：:Phosphotransacetylase and acetate kinase catalyze the activation of acetate to acetyl coenzyme A in the first step of methanogenesis from acetate in Methanosarcina thermophila. The genes encoding these enzymes (pta and ack) have been cloned and sequenced. They are arranged on the chromosome with pta upstream of ack (M...

journal_title：Journal of bacteriology

authors： Singh-Wissmann K,Ferry JG

更新日期：1995-04-01 00:00:00

abstract：:Chorpenning, Frank W. (The Ohio State University, Columbus), and Matthew C. Dodd. Heterogenetic antigens of gram-positive bacteria. J. Bacteriol. 91:1440-1445. 1966.-Soluble antigens obtained by various methods from gram-positive bacteria were used to modify erythrocytes whose hemagglutinating reactions with immune ra...

journal_title：Journal of bacteriology

authors： Chorpenning FW,Dodd MC

更新日期：1966-04-01 00:00:00

abstract：:The ultimate membrane localization and function of most of the 185 predicted Pseudomonas aeruginosa PAO1 lipoproteins remain unknown. We constructed a fluorescent lipoprotein, CSFP(OmlA)-ChFP, by fusing the signal peptide and the first four amino acids of the P. aeruginosa outer membrane lipoprotein OmlA to the monome...

journal_title：Journal of bacteriology

authors： Lewenza S,Mhlanga MM,Pugsley AP

更新日期：2008-09-01 00:00:00

abstract：:In Escherichia coli, the three branched-chain amino acid activating enzymes appear to be essential for multivalent repression of the isoleucine- and valine-forming enzymes. The results of experiments with a mutant, strain CU18, having an altered threonine deaminase, indicate that free isoleucine and some form of threo...

journal_title：Journal of bacteriology

authors： Wasmuth JJ,Umbarger HE

更新日期：1974-01-01 00:00:00

abstract：:Bacillus brevis HPD31 contains a surface (S)-layer protein, termed the HWP, which forms a hexagonal array in the cell wall. The 5' region of the HWP gene was isolated from a DNA library constructed in bacteriophage vector EMBL3 from a partial BamHI digest of the chromosomal DNA. The 3' region contained in a 2.7-kiloba...

journal_title：Journal of bacteriology

authors： Ebisu S,Tsuboi A,Takagi H,Naruse Y,Yamagata H,Tsukagoshi N,Udaka S

更新日期：1990-03-01 00:00:00

abstract：UNLABELLED:Misincorporation of D-tyrosine (D-Tyr) into cellular proteins due to mischarging of tRNA(Tyr) with D-Tyr by tyrosyl-tRNA synthetase inhibits growth and biofilm formation of Bacillus subtilis. Furthermore, many B. subtilis strains lack a functional gene encoding D-aminoacyl-tRNA deacylase, which prevents misi...

journal_title：Journal of bacteriology

authors： Williams-Wagner RN,Grundy FJ,Raina M,Ibba M,Henkin TM

更新日期：2015-05-01 00:00:00

abstract：:Cell wall turnover was examined in parent and mutant strains of Staphylococcus aureus. Peptidoglycan and teichoic acid were observed to undergo turnover in the wild-type strain during exponential growth; however, the rate of turnover did not decrease when the growth rate slowed, as the culture entered stationary phase...

journal_title：Journal of bacteriology

authors： Wong W,Chatterjee AN,Young FE

更新日期：1978-05-01 00:00:00

abstract：UNLABELLED:Resistance to methicillin and other β-lactam antibiotics in staphylococci is due to mecA, which is carried on a genomic island, staphylococcal cassette chromosome mec (SCCmec). The chromosomal excision and integration of SCCmec are mediated by the site-specific recombinase CcrAB or CcrC, encoded within this ...

journal_title：Journal of bacteriology

authors： Wang L,Ahmed MH,Safo MK,Archer GL

更新日期：2015-09-01 00:00:00

abstract：:Sixty-four mutants were isolated from the LT-2 wild-type strain of Salmonella typhimurium by selecting for chromate resistance. The majority of lesions were shown to lie in the cysA gene. (i) The mutants cannot take up sulfate, a finding which verifies the role of cysA in sulfate transport. In addition, 52 sulfate-tra...

journal_title：Journal of bacteriology

authors： Ota N,Galsworthy PR,Pardee AB

更新日期：1971-03-01 00:00:00

abstract：:We previously elucidated the global transcriptional responses of Escherichia coli to the nitrosating agent S-nitrosoglutathione (GSNO) in both aerobic and anaerobic chemostats, demonstrated the expression of nitric oxide (NO)-protective mechanisms, and obtained evidence of critical thiol nitrosation. The present study...

journal_title：Journal of bacteriology

authors： Pullan ST,Gidley MD,Jones RA,Barrett J,Stevanin TM,Read RC,Green J,Poole RK

更新日期：2007-03-01 00:00:00

abstract：:The β-barrel assembly machinery (BAM) complex is the core machinery for the assembly of β-barrel membrane proteins, and inhibition of BAM complex activity is lethal to bacteria. Discovery of integral membrane proteins that are key to pathogenesis and yet do not require assistance from the BAM complex raises the questi...

journal_title：Journal of bacteriology

authors： Hay ID,Belousoff MJ,Dunstan RA,Bamert RS,Lithgow T

更新日期：2018-02-07 00:00:00

abstract：:Protein export in Escherichia coli is mediated by translocase, a multisubunit membrane protein complex with SecA as the peripheral subunit and the SecY, SecE, and SecG proteins as the integral membrane domain. In the gram-positive bacterium Bacillus subtilis, SecA, SecY, and SecE have been identified through genetic a...

journal_title：Journal of bacteriology

authors： van Wely KH,Swaving J,Broekhuizen CP,Rose M,Quax WJ,Driessen AJ

更新日期：1999-03-01 00:00:00

abstract：:Transcription of the trp operon of Bacillus subtilis is regulated by attenuation. A trpE'-'lacZ gene fusion preceded by the wild-type trp promoter-leader region was used to analyze regulation. Overproduction of the trp leader transcript in trans from a multicopy plasmid caused constitutive expression of the chromosoma...

journal_title：Journal of bacteriology

authors： Kuroda MI,Henner D,Yanofsky C

更新日期：1988-07-01 00:00:00

abstract：:Studies on the active product of pepsin digestion of rabbit immunoglobulin G (IgG), F(ab')(2) produced from antiserum against Neurospora glutamate dehydrogenase, showed it to behave in the same way, in both antienzyme and precipitation experiments, as homologous IgG. It is proposed that the inhibition of glutamate deh...

journal_title：Journal of bacteriology

authors： Roberts DB

更新日期：1967-10-01 00:00:00

abstract：:For the transformation of the yeast species Kluyveromyces fragilis, we have constructed a vector containing a bacterial kanamycin resistance (Kmr) gene, the TRP1 gene of Saccharomyces cerevisiae, and an autonomously replicating sequence of Kluyveromyces lactis called KARS2 . By utilizing the method based on treatment ...

journal_title：Journal of bacteriology

authors： Das S,Kellermann E,Hollenberg CP

更新日期：1984-06-01 00:00:00

abstract：:The herpes simplex virus thymidine kinase gene has been cloned into a chimeric yeast plasmid cloning vehicle and transformed into appropriate yeast strains. Plasmids carrying the herpes simplex virus thymidine kinase gene can be propagated as autonomously replicating plasmids, but no RNA specific to the thymidine kina...

journal_title：Journal of bacteriology

authors： Kiss GB,Pearlman RE,Cornish KV,Friesen JD,Chan VL

更新日期：1982-02-01 00:00:00

abstract：:Two related killer strains of Saccharomyces cerevisiae were mutagenized and screened for nonkiller variants. About 20% of the mutants derived from one strain lacked all detectable double-straned ribonucleic acid (dsRNA). About 70% of the mutants from the other strain lacked one of the dsRNA species normally associated...

journal_title：Journal of bacteriology

authors： Vodkin M

更新日期：1977-10-01 00:00:00

abstract：:A new insertion element, IS1549, was identified serendipitously from Mycobacterium smegmatis LR222 during experiments using a vector designed to detect the excision of IS6110 from between the promoter region and open reading frame (ORF) of an aminoglycoside phosphotransferase gene. Six of the kanamycin-resistant isola...

journal_title：Journal of bacteriology

authors： Plikaytis BB,Crawford JT,Shinnick TM

更新日期：1998-03-01 00:00:00

abstract：:Although amino acid transport has been extensively studied in bacteria during the past decade, little is known concerning the transport of those amino acids that are biosynthetic intermediates or have multiple fates within the cell. We have studied homoserine and threonine as examples of this phenomenon. Homoserine is...

journal_title：Journal of bacteriology

authors： Templeton BA,Savageau MA

更新日期：1974-03-01 00:00:00

abstract：:Three additional ATPase genes, clustered in the order ahaH, ahaI, and ahaK, were found upstream of the previously characterized genes ahaECFABDG coding for the archaeal A1A0 ATPase from Methanosarcina mazei. ahaH, the first gene in the cluster, is preceded by a conserved promoter sequence. Northern blot analysis revea...

journal_title：Journal of bacteriology

authors： Ruppert C,Wimmers S,Lemker T,Müller V

更新日期：1998-07-01 00:00:00

abstract：:Membrane vesicles isolated from cells of bacillus subtilis W23 accumulate manganese in the presence of an energy source. The artificial electron donor system ascorbate and phenazine methosulfate or reduced nicotinamide adenine dinucleotide and phenazine methosulfate can supply the energy for the uptake. D-Lactate in t...

journal_title：Journal of bacteriology

authors： Bhattacharyya P

更新日期：1975-07-01 00:00:00

abstract：:top mutations (formerly supX) eliminate DNA topoisomerase I activity and suppress the leu-500 promoter mutation in Salmonella typhimurium (K. M. Overbye, S. K. Basu, and P. Margolin, Cold Spring Harbor Symp. Quant. Biol. 47:785-791, 1983). Sublethal doses of coumermycin which reduce intracellular levels of supercoilin...

journal_title：Journal of bacteriology

authors： Pruss GJ,Drlica K

更新日期：1985-11-01 00:00:00