DNA supercoiling and suppression of the leu-500 promoter mutation.

Abstract:

:top mutations (formerly supX) eliminate DNA topoisomerase I activity and suppress the leu-500 promoter mutation in Salmonella typhimurium (K. M. Overbye, S. K. Basu, and P. Margolin, Cold Spring Harbor Symp. Quant. Biol. 47:785-791, 1983). Sublethal doses of coumermycin which reduce intracellular levels of supercoiling activity in a top mutant eliminated suppression of the leu-500 mutation. This result provides evidence that increased DNA supercoiling suppresses the leu-500 promoter mutation in top mutants.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Pruss GJ,Drlica K

doi

10.1128/JB.164.2.947-949.1985

subject

Has Abstract

pub_date

1985-11-01 00:00:00

pages

947-9

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

164

pub_type

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