Regulation of Escherichia coli RelA requires oligomerization of the C-terminal domain.

abstract：:Insertion-duplication mutagenesis was used to generate mutants of Streptococcus pneumoniae that produced truncated forms of PspA (pneumococcal surface protein A). The truncated products, representing from 20 to 80% of the complete PspA molecule, were all secreted from the cell and could be detected in unconcentrated c...

journal_title：Journal of bacteriology

authors： Yother J,Handsome GL,Briles DE

更新日期：1992-01-01 00:00:00

abstract：:The incA gene product of ColE2-P9 and ColE3-CA38 plasmids is an antisense RNA that regulates the production of the plasmid-coded Rep protein essential for replication. The Rep protein specifically binds to the origin and synthesizes a unique primer RNA at the origin. The IncB incompatibility is due to competition for ...

journal_title：Journal of bacteriology

authors： Hiraga S,Sugiyama T,Itoh T

更新日期：1994-12-01 00:00:00

abstract：:Among mutants which require isoleucine, but not valine, for growth, we have found two distinguishable classes. One is defective in the biosynthetic enzyme threonine deaminase (l-threonine hydro-lyase, deaminating, EC 4.2.1.16) and the other has an altered isoleucyl transfer ribonucleic acid (tRNA) synthetase [l-isoleu...

journal_title：Journal of bacteriology

authors： Iaccarino M,Berg P

更新日期：1971-02-01 00:00:00

abstract：:Comamonas sp. strain CNB-1 degrades nitrobenzene and chloronitrobenzene via the intermediates 2-aminomuconate and 2-amino-5-chloromuconate, respectively. Deamination of these two compounds results in the release of ammonia, which is used as a source of nitrogen for bacterial growth. In this study, a novel deaminase wa...

journal_title：Journal of bacteriology

authors： Liu L,Wu JF,Ma YF,Wang SY,Zhao GP,Liu SJ

更新日期：2007-04-01 00:00:00

abstract：:Xylella fastidiosa is a xylem-limited nonflagellated bacterium that causes economically important diseases of plants by developing biofilms that block xylem sap flow. How the bacterium is translocated downward in the host plant's vascular system against the direction of the transpiration stream has long been a puzzlin...

journal_title：Journal of bacteriology

authors： Meng Y,Li Y,Galvani CD,Hao G,Turner JN,Burr TJ,Hoch HC

更新日期：2005-08-01 00:00:00

abstract：:We report the first study of tRNA modification in psychrotolerant archaea, specifically in the archaeon Methanococcoides burtonii grown at 4 and 23 degrees C. For comparison, unfractionated tRNA from the archaeal hyperthermophile Stetteria hydrogenophila cultured at 93 degrees C was examined. Analysis of modified nucl...

journal_title：Journal of bacteriology

authors： Noon KR,Guymon R,Crain PF,McCloskey JA,Thomm M,Lim J,Cavicchioli R

更新日期：2003-09-01 00:00:00

abstract：:We have evaluated the use of [1,2-13C2]propionate for the analysis of propionic acid metabolism, based on the ability to distinguish between the methylcitrate and methylmalonate pathways. Studies using propionate-adapted Escherichia coli MG1655 cells were performed. Preservation of the 13C-13C-12C carbon skeleton in l...

journal_title：Journal of bacteriology

authors： London RE,Allen DL,Gabel SA,DeRose EF

更新日期：1999-06-01 00:00:00

abstract：:An abundant 19.3-kDa Helicobacter pylori protein has been cloned, and the sequence is homologous with a ferritin-like protein produced by Escherichia coli K-12. Homologies are also present with a number of eucaryotic ferritins, as well as with the heme group-containing bacterioferritins. All amino acids involved in ch...

journal_title：Journal of bacteriology

authors： Frazier BA,Pfeifer JD,Russell DG,Falk P,Olsén AN,Hammar M,Westblom TU,Normark SJ

更新日期：1993-02-01 00:00:00

abstract：:Screening of derivatives of Rhizobium etli KIM5s randomly mutagenized with mTn5SSgusA30 resulted in the identification of strain KIM-G1. Its rough colony appearance, flocculation in liquid culture, and Ndv(-) Fix(-) phenotype were indicative of a lipopolysaccharide (LPS) defect. Electrophoretic analysis of cell-associ...

journal_title：Journal of bacteriology

authors： Vinuesa P,Reuhs BL,Breton C,Werner D

更新日期：1999-09-01 00:00:00

abstract：:The role of the DNA primase of IncP plasmids was examined with a derivative of RP4 containing Tn7 in the primase gene (pri). The mutant was defective in mediating bacterial conjugation, with the deficiency varying according to the bacterial strains used as donors and recipients. Complementation tests involving recombi...

journal_title：Journal of bacteriology

authors： Merryweather A,Barth PT,Wilkins BM

更新日期：1986-07-01 00:00:00

abstract：:The properties of homogeneous preparations of carbamoylphosphate synthetase (CPSase) from wild-type Salmonella typhimurium and a cold-sensitive derivative grown at different growth temperatures were examined. For the cold-sensitive mutant, the affinity for glutamine of the form of CPSase synthesized at 20 degrees C wa...

journal_title：Journal of bacteriology

authors： Han BD,Nolan WG,Hopkins HP,Jones RT,Ingraham JL,Abdelal AT

更新日期：1990-09-01 00:00:00

abstract：:Both the acridine half-mustard, ICR191, and the nonalkylating azaacridine derivative, ICR364-OH, induce three classes of frameshift mutations in the histidine operon of Salmonella typhimurium. (i) One class is completely stable in reversion tests and is presumed to represent deletion of one or a few critical nucleotid...

journal_title：Journal of bacteriology

authors： Oeschger NS,Hartman PE

更新日期：1970-02-01 00:00:00

abstract：:Goldberg, Herbert S. (University of Missouri, Columbia), Ella M. Barnes, and Anthony B. Charles. Unusual Bacteroides-like organism. J. Bacteriol. 87:737-742. 1964.-An organism is described which appears to be a new species of gram-negative, anaerobic, nonsporulating rod. It was isolated from poultry caeca at levels of...

journal_title：Journal of bacteriology

authors： GOLDBERG HS,BARNES EM,CHARLES AB

更新日期：1964-03-01 00:00:00

abstract：:All Escherichia coli strains so far examined possess a chromosomally encoded nanATEK-yhcH operon for the catabolism of sialic acids. These unique nine-carbon sugars are synthesized primarily by higher eukaryotes and can be used as carbon, nitrogen, and energy sources by a variety of microbial pathogens or commensals. ...

journal_title：Journal of bacteriology

authors： Kalivoda KA,Steenbergen SM,Vimr ER,Plumbridge J

更新日期：2003-08-01 00:00:00

abstract：:Cells of Pseudomonas aeruginosa resistant to benzalkonium chloride (BC) underwent unique ultrastructural reorganizations when they were grown in the presence of 1 mg of BC/ml. The resistant cells usually contained a single, centrally positioned pseudovacuole. The pseudovacuole was surrounded by a diffuse substance tha...

journal_title：Journal of bacteriology

authors： Hoffmann HP,Geftic SG,Gelzer J,Heymann H,Adair FW

更新日期：1973-01-01 00:00:00

abstract：:Vegetative cells and cysts of Azotobacter vinelandii 12837 were prepared for electron microscopy by several methods assumed to preserve structural details destroyed by techniques previously reported in the literature. Examination of large numbers of cells and cysts by these methods revealed four structural details not...

journal_title：Journal of bacteriology

authors： Vela GR,Cagle GD,Holmgren PR

更新日期：1970-11-01 00:00:00

abstract：:Competent cell-deoxyribonucleic acid (DNA) interactions were examined using tritium-labeled homologous or heterologous native or denatured DNAs and competent Streptococcus sanguis Wicky cells (strain WE4). The DNAs used were extracted from WE4 cells, Escherichia coli B cells, and E. coli bacteriophages T2, T4, T6, and...

journal_title：Journal of bacteriology

authors： Ranhand JM

更新日期：1980-05-01 00:00:00

abstract：:The Yersinia pestis proteome was studied as a function of temperature and calcium by two-dimensional differential gel electrophoresis. Over 4,100 individual protein spots were detected, of which hundreds were differentially expressed. A total of 43 differentially expressed protein spots, representing 24 unique protein...

journal_title：Journal of bacteriology

authors： Chromy BA,Choi MW,Murphy GA,Gonzales AD,Corzett CH,Chang BC,Fitch JP,McCutchen-Maloney SL

更新日期：2005-12-01 00:00:00

abstract：:In Bacillus thuringiensis, a novel N-acetylmuramoyl-L-alanine amidase gene (named cwlB) was detected, and the CwlB protein was purified and characterized. Reverse transcription-PCR (RT-PCR) results indicated that cwlB and an upstream gene (named cwlA) formed one transcriptional unit. 5' rapid amplification of cDNA end...

journal_title：Journal of bacteriology

authors： Yang J,Peng Q,Chen Z,Deng C,Shu C,Zhang J,Huang D,Song F

更新日期：2013-06-01 00:00:00

abstract：:Short-chain acid-soluble polyphosphates were extracted from growing cultures of Saccharomyces cerevisiae, and the changes in the levels of these compounds were determined. The production of acid-soluble polyphosphates correlated with the mitochondrial activities since it occurred in two bursts in respiration-competent...

journal_title：Journal of bacteriology

authors： Solimene R,Guerrini AM,Donini P

更新日期：1980-08-01 00:00:00

abstract：:Expression levels of Bacillus amyloliquefaciens alpha-amylase, Escherichia coli TEM-beta-lactamase, and Semliki Forest virus glycoprotein E1 genes were compared in Bacillus subtilis. All three model genes were expressed by using a secretion vector, constructed by joining the B. amyloliquefaciens alpha-amylase promoter...

journal_title：Journal of bacteriology

authors： Ulmanen I,Lundström K,Lehtovaara P,Sarvas M,Ruohonen M,Palva I

更新日期：1985-04-01 00:00:00

abstract：:The tuf gene which encodes peptide chain elongation factor Tu was found to be duplicated in nine enteric and four nonenteric gram-negative bacteria, but present only in one copy in two gram-positive genera. In two of the nonenteric gram-negative genera, Pseudomonas and Caulobacter, the duplicate tuf genes were found t...

journal_title：Journal of bacteriology

authors： Filer D,Furano AV

更新日期：1981-12-01 00:00:00

abstract：:Legionella pneumophila expresses two peroxide-scavenging alkyl hydroperoxide reductase systems (AhpC1 and AhpC2D) that are expressed differentially during the bacterial growth cycle. Functional loss of the postexponentially expressed AhpC1 system is compensated for by increased expression of the exponentially expresse...

journal_title：Journal of bacteriology

authors： LeBlanc JJ,Brassinga AK,Ewann F,Davidson RJ,Hoffman PS

更新日期：2008-05-01 00:00:00

abstract：:The butyrogenic genes from Clostridium difficile DSM 1296(T) have been cloned and expressed in Escherichia coli. The enzymes acetyl-coenzyme A (CoA) C-acetyltransferase, 3-hydroxybutyryl-CoA dehydrogenase, crotonase, phosphate butyryltransferase, and butyrate kinase and the butyryl-CoA dehydrogenase complex composed o...

journal_title：Journal of bacteriology

authors： Aboulnaga el-H,Pinkenburg O,Schiffels J,El-Refai A,Buckel W,Selmer T

更新日期：2013-08-01 00:00:00

abstract：:Synthesis of heme, measured by incorporation of iron-59, and of bacteriochlorophyll was studied with wild-type and mutant strains of Rhodopseudomonas spheroides. The wild type formed heme from glycine and succinate at one-fortieth the rate of bacteriochlorophyll under anaerobic-light conditions. Added delta-aminolevul...

journal_title：Journal of bacteriology

authors： Lascelles J,Hatch TP

更新日期：1969-05-01 00:00:00

abstract：:The extent and nature of DNA polymorphism in the mutS-rpoS region of the Escherichia coli genome were assessed in 21 strains of enteropathogenic E. coli (EPEC) and enterohemorrhagic E. coli (EHEC) and in 6 strains originally isolated from natural populations. The intervening region between mutS and rpoS was amplified ...

journal_title：Journal of bacteriology

authors： Herbelin CJ,Chirillo SC,Melnick KA,Whittam TS

更新日期：2000-10-01 00:00:00

abstract：:The transduction efficiency of Tn551, a staphylococcal transposon coding for erythromycin resistance (ermB), was increased by a factor of about five in acceptor strains carrying the gene for methicillin resistance (mec), as compared with methicillin-sensitive strains. This enhancement was independent of the generalize...

journal_title：Journal of bacteriology

authors： Berger-Bächi B

更新日期：1983-04-01 00:00:00

abstract：:The penicillin-binding proteins (PBPs) are a set of enzymes that participate in bacterial peptidoglycan assembly. The absolute numbers of each PBP were determined by direct measurement and have been reported for two Staphylococcus aureus strains, RN4220 (methicillin-sensitive S. aureus) and RN450M (methicillin-resista...

journal_title：Journal of bacteriology

authors： Pucci MJ,Dougherty TJ

更新日期：2002-01-01 00:00:00

abstract：:PsaA, the subunit of the fimbria originally referred to as the "pH 6 antigen," is required for full virulence of Yersinia pestis during bubonic plague. The expression of psaA is dependent upon specific environmental signals, and while the signals (high temperature and acidic pH) are defined, the mechanisms underlying ...

journal_title：Journal of bacteriology

authors： Quinn JD,Weening EH,Miner TA,Miller VL

更新日期：2019-07-24 00:00:00

abstract：:We analyzed 12 individual codons, which differed widely with respect to the frequency of use in Escherichia coli and the abundance of the corresponding tRNAs, for their influence on the coupling between transcription and translation. This was probed by determining the effects of codon substitutions in the leader pepti...

journal_title：Journal of bacteriology

authors： Bonekamp F,Dalbøge H,Christensen T,Jensen KF

更新日期：1989-11-01 00:00:00