Transcription and translation of foreign genes in Bacillus subtilis by the aid of a secretion vector.

abstract：:A mutant of Pseudomonas aeruginosa severely deficient in outer membrane protein F levels was isolated by screening heavily mutagenized strains for membrane protein alterations on sodium dodecyl sulphate-polyacrylamide gel electrophoresis. To provide a basis for phenotypic comparison, three independent spontaneous reve...

journal_title：Journal of bacteriology

authors： Nicas TI,Hancock RE

更新日期：1983-01-01 00:00:00

abstract：:Attaching and effacing (AE) pathogens colonize the gut mucosa using a type three secretion system (T3SS) and a suite of effector proteins. The locus of enterocyte effacement (LEE) is the defining genetic feature of the AE pathogens, encoding the T3SS and the core effector proteins necessary for pathogenesis. Extensive...

journal_title：Journal of bacteriology

authors： Furniss RCD,Clements A

更新日期：2017-12-20 00:00:00

abstract：:The genetic requirements for the excision repair of thymine glycols, urea residues, and apurinic (AP) sites were examined by measuring the survival in Escherichia coli mutants of phi X174 replicative form (RF) I transfecting DNA containing selectively introduced lesions. phi X RF I DNA containing thymine glycols was i...

journal_title：Journal of bacteriology

authors： Laspia MF,Wallace SS

更新日期：1988-08-01 00:00:00

abstract：:Membrane vesicles isolated from cells of bacillus subtilis W23 accumulate manganese in the presence of an energy source. The artificial electron donor system ascorbate and phenazine methosulfate or reduced nicotinamide adenine dinucleotide and phenazine methosulfate can supply the energy for the uptake. D-Lactate in t...

journal_title：Journal of bacteriology

authors： Bhattacharyya P

更新日期：1975-07-01 00:00:00

abstract：:Cellular pigmentation is an important virulence factor of the oral pathogen Porphyromonas gingivalis Pigmentation has been associated with many bacterial functions, including but not limited to colonization, maintaining a local anaerobic environment by binding oxygen molecules, and defense against reactive oxygen spec...

journal_title：Journal of bacteriology

authors： Klein BA,Cornacchione LP,Collins M,Malamy MH,Duncan MJ,Hu LT

更新日期：2017-06-27 00:00:00

abstract：:Yohn, David S. (Roswell Park Memorial Institute, Buffalo, N.Y.), Victoria A. Haendiges, and James T. Grace, Jr. Yaba tumor poxvirus synthesis in vitro. I. Cytopathological, histochemical, and immunofluorescent studies. J. Bacteriol. 91:1977-1985. 1966.-Yaba tumor poxvirus synthesis in BSC-1 cell culture was followed s...

journal_title：Journal of bacteriology

authors： Yohn DS,Haendiges VA,Grace JT Jr

更新日期：1966-05-01 00:00:00

abstract：:Escherichia coli strains lysogenic for a thermoinducible Mu prophage (Mu cts62) undergo rapid lysis about 50 min after heat induction. Induction of Mu cts62 apparently causes damage to the host sequences in which Mu is inserted. The normal expression of A, BU, and X genes of Mu is needed for this specific deleterious ...

journal_title：Journal of bacteriology

authors： Razzaki T,Bukhari AI

更新日期：1975-05-01 00:00:00

abstract：:Deletion mutagenesis analysis of a duplicated gene necessary for Haemophilus influenzae serotype b capsule expression showed that only one functional copy of this gene is required for capsule production and for virulence in infant rats. Mutant strains generated in this study differed from each other and from the paren...

journal_title：Journal of bacteriology

authors： Ely S,Tippett J,Kroll JS,Moxon ER

更新日期：1986-07-01 00:00:00

abstract：:The hemA mutation of Escherichia coli K-12 confers a requirement for delta-aminolevulinic acid (ALA). Cell extract prepared from the hemA strain SASX41B was incapable of producing ALA from either glutamate or glutamyl-tRNA, whereas extract of the hem+ strain HB101 formed colorimetrically detectable amounts of ALA and ...

journal_title：Journal of bacteriology

authors： Avissar YJ,Beale SI

更新日期：1989-06-01 00:00:00

abstract：:The McrB restriction system of Escherichia coli K-12 is responsible for the biological inactivation of foreign DNA that contains 5-methylcytosine residues (E. A. Raleigh and G. Wilson, Proc. Natl. Acad. Sci. USA 83:9070-9074, 1986). Within the McrB region of the chromosome is the mcrB gene, which encodes a protein of ...

journal_title：Journal of bacteriology

authors： Ross TK,Achberger EC,Braymer HD

更新日期：1989-04-01 00:00:00

abstract：:The recA gene from Thermus thermophilus HB27 was cloned and engineered to obtain insertion (recA::kat) and deletion (deltarecA) derivatives. Transcription of recA in this extreme thermophile was induced by mitomycin C, leading to the synthesis of a monocistronic mRNA. This DNA damage-mediated induction was dependent o...

journal_title：Journal of bacteriology

authors： Castán P,Casares L,Barbé J,Berenguer J

更新日期：2003-08-01 00:00:00

abstract：:The cis-acting regulatory regions of the tps and ops genes of Myxococcus xanthus were localized by analyzing the expression of fusions of these genes with the lacZ gene. A 201-base-pair (bp) fragment of tps DNa extending 95 bp upstream (-95) from the transcriptional start was sufficient to direct developmentally regul...

journal_title：Journal of bacteriology

authors： Downard JS,Kim SH,Kil KS

更新日期：1988-10-01 00:00:00

abstract：:A 6.2-kb region of DNA corresponding to complementation groups II and III of the Erwinia amylovora hrp gene cluster was analyzed. Transposon mutagenesis indicated that the two complementation groups are required for secretion of harpin, an elicitor of the hypersensitive reaction. The sequence of the region revealed 10...

journal_title：Journal of bacteriology

authors： Kim JF,Wei ZM,Beer SV

更新日期：1997-03-01 00:00:00

abstract：:Escherichia coli O55 is an important antigen which is often associated with enteropathogenic E. coli clones. We sequenced the genes responsible for its synthesis and identified genes for O-antigen polymerase, O-antigen flippase, four enzymes involved in GDP-colitose synthesis, and three glycosyltransferases, all by co...

journal_title：Journal of bacteriology

authors： Wang L,Huskic S,Cisterne A,Rothemund D,Reeves PR

更新日期：2002-05-01 00:00:00

abstract：:Lactobacillus fructivorans is important in the generation of particular flavors and in other ripening processes associated with fermented food. Here, we present the draft genome sequence of the type strain Lactobacillus fructivorans KCTC 3543 (1,373,326 bp, with a G+C content of 38.9%), which consists of 5 scaffolds. ...

journal_title：Journal of bacteriology

authors： Nam SH,Choi SH,Kang A,Lee KS,Kim DW,Kim RN,Kim DS,Park HS

更新日期：2012-04-01 00:00:00

abstract：:We performed a mutational analysis of the left half of Tn10-encoded tet operator O2, located in the 5' nontranslated region of the mRNA for the resistance protein TetA, and determined the importance of that region for translation efficiency and mRNA stability. Transcriptional fusions of 17 mutants to lacZ expressed th...

journal_title：Journal of bacteriology

authors： Flache P,Baumeister R,Hillen W

更新日期：1992-04-01 00:00:00

abstract：:Decreased intracellular levels of FtsH, a membrane-bound ATPase, led to retardation of growth and protein export, as well as to an abnormal translocation of alkaline phosphatase that had been attached to a cytoplasmic domain of a multispanning membrane protein, SecY. The last phenotype is designated Std (stop transfer...

journal_title：Journal of bacteriology

authors： Shirai Y,Akiyama Y,Ito K

更新日期：1996-02-01 00:00:00

abstract：:A fusion between the pCloDF13-derived bacteriocin release protein and beta-lactamase was constructed to investigate the subcellular localization and posttranslational modification of the bacteriocin release protein in Escherichia coli. The signal sequence and 25 of the 28 amino acid residues of the mature bacteriocin ...

journal_title：Journal of bacteriology

authors： Luirink J,Watanabe T,Wu HC,Stegehuis F,de Graaf FK,Oudega B

更新日期：1987-05-01 00:00:00

abstract：:Azotobacter vinelandii DJ71, which contains a mutation in the nifV gene, was derepressed for nitrogenase in the presence of homocitrate. When dinitrogenase was isolated from this culture, it was found to be identical to the wild-type dinitrogenase. However, when the same NifV- strain was derepressed in the presence of...

journal_title：Journal of bacteriology

authors： Madden MS,Paustian TD,Ludden PW,Shah VK

更新日期：1991-09-01 00:00:00

abstract：:The gene that encodes the acyl carrier protein (ACP) of the actinorhodin polyketide synthase (PKS) of Streptomyces coelicolor A3(2) was replaced with homologs from the granaticin, oxytetracycline, tetracenomycin, and putative frenolicin polyketide synthase gene clusters. All of the replacements led to expression of fu...

journal_title：Journal of bacteriology

authors： Khosla C,McDaniel R,Ebert-Khosla S,Torres R,Sherman DH,Bibb MJ,Hopwood DA

更新日期：1993-04-01 00:00:00

abstract：:Genes from Bacillus subtilis predicted to encode a phosphate-specific transport (Pst) system were shown by mutation to affect high-affinity Pi uptake but not arsenate resistance or phosphate (Pho) regulation. The transcription start of the promoter upstream of the pstS gene was defined by primer extension. The promote...

journal_title：Journal of bacteriology

authors： Qi Y,Kobayashi Y,Hulett FM

更新日期：1997-04-01 00:00:00

abstract：:The nucleotide sequence of a region located downstream of the nifB gene, both in the cyanobacterium Anabaena sp. strain PCC 7120 and in Rhizobium meliloti, has been determined. This region contains a gene (fdxN) whose predicted polypeptide product strongly resembles typical bacterial ferredoxins. Cyanobacteria have no...

journal_title：Journal of bacteriology

authors： Mulligan ME,Buikema WJ,Haselkorn R

更新日期：1988-09-01 00:00:00

abstract：:Strain IMCC3088, cultivated from the Yellow Sea, is a novel isolate belonging to the OM60/NOR5 clade and is closely related to clone OM241, Congregibacter litoralis, and strain HTCC2080. Here, the genome sequence of strain IMCC3088 is presented, showing the absence of photosynthetic gene clusters and the presence of p...

journal_title：Journal of bacteriology

authors： Jang Y,Oh HM,Kang I,Lee K,Yang SJ,Cho JC

更新日期：2011-07-01 00:00:00

abstract：:Gel retardation experiments indicated the presence in Pseudomonas aeruginosa cell extracts of an arginine-inducible DNA-binding protein that interacts with the control regions for the car and argF operons, encoding carbamoylphosphate synthetase and anabolic ornithine carbamoyltransferase, respectively. Both enzymes ar...

journal_title：Journal of bacteriology

authors： Park SM,Lu CD,Abdelal AT

更新日期：1997-09-01 00:00:00

abstract：:The yfdXWUVE operon appears to encode proteins that enhance the ability of Escherichia coli MG1655 to survive under acidic conditions. Although the molecular mechanisms underlying this phenotypic behavior remain to be elucidated, findings from structural genomic studies have shown that the structure of YfdW, the prote...

journal_title：Journal of bacteriology

authors： Toyota CG,Berthold CL,Gruez A,Jónsson S,Lindqvist Y,Cambillau C,Richards NG

更新日期：2008-04-01 00:00:00

abstract：:Several mutants of Bacillus subtilis deficient in catalase synthesis generated by nitrosoguanidine mutagenesis have been used to map a locus affecting catalase activity. Two- and three-factor bacteriophage PBS1 transductional crosses were used to locate the locus, named katA, between recH and thiA with 98% linkage to ...

journal_title：Journal of bacteriology

authors： Loewen PC,Switala J

更新日期：1987-12-01 00:00:00

abstract：:Myxobacteria are predatory and are prolific producers of secondary metabolites. Here, we tested a hypothesized role that secondary metabolite antibiotics function as weapons in predation. To test this, a Myxococcus xanthus Δta1 mutant, blocked in antibiotic TA (myxovirescin) production, was constructed. This TA(-) mut...

journal_title：Journal of bacteriology

authors： Xiao Y,Wei X,Ebright R,Wall D

更新日期：2011-09-01 00:00:00

abstract：:The Hly region from the chromosome of Vibrio cholerae El Tor strain RV79(Hly-) and the nonhemolytic classical strain 569B were cloned into plasmid vector pBR322. Escherichia coli K-12 transformants possessing these recombinant plasmids were nonhemolytic and were detected with a 32P-labeled hly-specific DNA probe. Rest...

journal_title：Journal of bacteriology

authors： Goldberg SL,Murphy JR

更新日期：1985-04-01 00:00:00

abstract：:Movement on surfaces, or swarming motility, is effectively mediated by the lateral flagellar (laf) system in Vibrio parahaemolyticus. Expression of laf is induced by conditions inhibiting rotation of the polar flagellum, which is used for swimming in liquid. However, not all V. parahaemolyticus isolates swarm proficie...

journal_title：Journal of bacteriology

authors： Jaques S,McCarter LL

更新日期：2006-04-01 00:00:00

abstract：:A map location of the gluconate-6-phosphate dehydrogenase (gnd) marker was estimated in Escherichia coli C at approximately 46 min by P1 transduction. The gnd locus appears to lie between the co-transducible histidine and prophage P2 location I markers. ...

journal_title：Journal of bacteriology

authors： Robbins RS,Kelly B

更新日期：1973-11-01 00:00:00