Abstract:
:Both the acridine half-mustard, ICR191, and the nonalkylating azaacridine derivative, ICR364-OH, induce three classes of frameshift mutations in the histidine operon of Salmonella typhimurium. (i) One class is completely stable in reversion tests and is presumed to represent deletion of one or a few critical nucleotide pairs or two nearby frameshifts. One extended deletion was found out of 11 stable mutations. (ii) Of two spontaneously reverting classes which also are considered to predominantly involve base deletions, one is unaffected in reversion with ICR191, nitrosoguanidine, and diethylsulfate, and the other is induced to revert with ICR191. (iii) A third class, considered to predominantly involve base additions, responds in reversion tests with ICR191 as well as with nitrosoguanidine and diethylsulfate. Other investigators have shown that one mutant of this class is a "plus" frameshift and that nitrosoguanidine acts in reversion to delete a guanine plus cytosine base pair. Although such plus frameshifts are found with high frequency among mutations selected from acridine-treated bacteria or when strong selection pressure is applied for their detection in reversion tests, data from this laboratory indicate that this class of plus frameshifts is rare among mutations derived spontaneously or after treatment with a variety of other mutagens. Finally, we demonstrate that the alkylating ICR191 and the nonalkylating ICR364-OH preferentially cause mutations in different chromosome regions and that their spectra of activity only partially overlap that found for spontaneous frameshift mutations.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Oeschger NS,Hartman PEdoi
10.1128/JB.101.2.490-504.1970subject
Has Abstractpub_date
1970-02-01 00:00:00pages
490-504issue
2eissn
0021-9193issn
1098-5530journal_volume
101pub_type
杂志文章abstract::The poly-beta-hydroxybutyrate (PHB) biosynthetic pathway from Alcaligenes eutrophus H16 has been cloned and expressed in Escherichia coli. Initially, an A. eutrophus H16 genomic library was constructed by using cosmid pVK102, and cosmid clones that encoded the PHB biosynthetic pathway were sought by assaying for the f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.10.4431-4436.1988
更新日期:1988-10-01 00:00:00
abstract::We report the draft genome sequence of Lactobacillus salivarius SMXD51, isolated from the cecum of healthy chickens showing an activity against Campylobacter--the food-borne pathogen that is the most common cause of gastroenteritis in the European Union (EU)--and potentially interesting features for a probiotic strain...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00344-12
更新日期:2012-06-01 00:00:00
abstract::The alpha (62,000-dalton) and beta (49,000-dalton) subunits of Methanosarcina barkeri ATPase were purified to homogeneity. The subunits and ATPase complex were trypsinized in the presence of various nucleotides. ATP and ADP changed the trypsin sensitivity of the alpha subunit in the complex and isolated forms, suggest...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.12.5960-5962.1988
更新日期:1988-12-01 00:00:00
abstract::Expression of the 146-kilodalton (kDa) extracellular glucoamylase by the budding yeast Schwanniomyces castellii is induced by maltose and starch. By use of antiglucoamylase antisera, we found that this expression was regulated at the level of the mRNA, taking place within 30 min after exposure of yeast cells to the re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.5.2360-2366.1990
更新日期:1990-05-01 00:00:00
abstract::Chloramphenicol-resistant strains of Staphylococcus aureus contain an inducible enzyme which inactivates chloramphenicol by acetylation in the presence of acetyl coenzyme A. The products of acetylation are chromatographically indistinguishable from those obtained with chloramphenicol-resistant Escherichia coli harbori...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.1.28-36.1968
更新日期:1968-01-01 00:00:00
abstract::Overexpression of vsr in Escherichia coli stimulates transition and frameshift mutations. The pattern of mutations suggests that mutagenesis is due to saturation or inactivation of dam-directed mismatch repair. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.14.4294-4296.1996
更新日期:1996-07-01 00:00:00
abstract::A gene in Legionella pneumophila that has significant homology to published hfq genes demonstrated regulation by RpoS and the transcriptional regulator LetA. Additionally, Hfq has a positive effect on the presence of transcripts of the genes for CsrA and the ferric uptake regulator Fur. Mutants lacking hfq demonstrate...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.4.1527-1532.2005
更新日期:2005-02-01 00:00:00
abstract::Expression of the catalase-peroxidase of Caulobacter crescentus, a gram-negative member of the alpha subdivision of the Proteobacteria, is 50-fold higher in stationary-phase cultures than in exponential cultures. To identify regulators of the starvation response, Tn5 insertion mutants were isolated with reduced expres...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.19.6152-6159.1999
更新日期:1999-10-01 00:00:00
abstract:UNLABELLED:TcpP and ToxR coordinately regulate transcription of toxT, the master regulator of numerous virulence factors in Vibrio cholerae. TcpP and ToxR are membrane-localized transcription factors, each with a periplasmic domain containing two cysteines. In ToxR, these cysteines form an intramolecular disulfide bond...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00338-15
更新日期:2015-11-16 00:00:00
abstract::The relationship between the levels of cyclic nucleotides and dimorphic transition in Candida albicans was examined. The results showed that cells of this pathogenic fungus contained both cyclic adenosine 3',5'-monophosphate (cAMP) and cyclic guanosine 3',5'-monophosphate (cGMP), the concentration of the latter being ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.142.3.1010-1014.1980
更新日期:1980-06-01 00:00:00
abstract::In contrast to Escherichia coli and Salmonella typhimurium, Bacillus subtilis could convert ethionine to S-adenosylethionine (SAE), as can Saccharomyces cerevisiae. This conversion was essential for growth inhibition by ethionine because metE mutants which were deficient in S-adenosylmethionine synthetase activity, we...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.1.1-8.1986
更新日期:1986-04-01 00:00:00
abstract:UNLABELLED:An ability to sense and respond to changes in extracellular phosphate is critical for the survival of most bacteria. For Caulobacter crescentus, which typically lives in phosphate-limited environments, this process is especially crucial. Like many bacteria, Caulobacter responds to phosphate limitation throug...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00658-15
更新日期:2015-10-19 00:00:00
abstract::The lactose-H+ symport protein (LacS) of Streptococcus thermophilus has a carboxyl-terminal regulatory domain (IIALacS) that is homologous to a family of proteins and protein domains of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) in various organisms, of which IIAGlc of Escherichia coli is the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.2.632-641.1999
更新日期:1999-01-01 00:00:00
abstract::Succinate dehydrogenase (EC 1.3.99.1) of Micrococcus luteus was selectively precipitated from Triton X-100-solubilized membranes by using specific antiserum. The precipitated enzyme contained equimolar amounts of four polypeptides with apparent molecular weights of 72,000, 30,000, 17,000, and 15,000. The 72,000 polype...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.3.1493-1501.1983
更新日期:1983-03-01 00:00:00
abstract::Reich, Paul R. (National Institutes of Health, Bethesda, Md.), Norman L. Somerson, James A. Rose, and Sherman M. Weissman. Genetic relatedness among mycoplasmas as determined by nucleic acid homology. J. Bacteriol. 91:153-160. 1966.-A sensitive membrane filter method to detect nucleic acid homology was used to determi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.1.153-160.1966
更新日期:1966-01-01 00:00:00
abstract::Short sequence repeats (SSRs), potentially representing variable numbers of tandem repeat (VNTR) loci, were identified for the human-pathogenic yeast species Candida albicans by computerized DNA sequence scanning. The individual SSR regions were investigated in different clinical isolates of C. albicans. Most of the C...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.15.3771-3778.1998
更新日期:1998-08-01 00:00:00
abstract::Plasmid pL32 from the Natto strain of Bacillus subtilis belongs to a group of low-copy-number plasmids in gram-positive bacteria that replicate via a theta mechanism of replication. We studied the DNA region encoding the replication protein, RepN, of pLS32, and obtained the following results. Transcription of the repN...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.13.4315-4326.2005
更新日期:2005-07-01 00:00:00
abstract::The protein compositions of the membrane preparations from Selenomonas ruminantium grown in glucose or lactate medium were determined by sodium dodecyl sulfate- and two-dimensional (first, isoelectric focusing; second, sodium dodecyl sulfate) polyacrylamide slab gel electrophoresis. The outer membrane from both glucos...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.2.899-907.1980
更新日期:1980-02-01 00:00:00
abstract::Sinorhizobium meliloti is a soil bacterium capable of invading and establishing a symbiotic relationship with alfalfa plants. This invasion process requires the synthesis, by S. meliloti, of at least one of the two symbiotically important exopolysaccharides, succinoglycan and EPS II. We have previously shown that the ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.1.325-331.2003
更新日期:2003-01-01 00:00:00
abstract::The effects of high doses of radiation (1, 5, or 20 Mrad) on the toxicity, pyrogenicity, and immunogenicity of Salmonella typhimurium cells and endotoxin were studied. Toxicity decreased progressively after exposure to 1, 5, or 20 Mrad. The lethal effect of 1-Mrad exposed cells was greater than that of heat-, acetone-...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.6.2165-2170.1968
更新日期:1968-06-01 00:00:00
abstract::As a step towards studying representative members of the two-component family of signal transduction proteins, we have cloned genes encoding a histidine protein kinase and a response regulator from the hyperthermophilic bacterium Thermotoga maritima. The genes have been designated HpkA and drrA, respectively. The dedu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.19.5579-5585.1996
更新日期:1996-10-01 00:00:00
abstract::Selenium has been shown to be present as a labile cofactor in a small class of molybdenum hydroxylase enzymes in several species of clostridia that specialize in the fermentation of purines and pyrimidines. This labile cofactor is poorly understood, yet recent bioinformatic studies have suggested that Enterococcus fae...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01063-10
更新日期:2011-04-01 00:00:00
abstract::The coccoid cyanobacterium Chroococcidiopsis dominates microbial communities in the most extreme arid hot and cold deserts. These communities withstand constraints that result from multiple cycles of drying and wetting and/or prolonged desiccation, through mechanisms which remain poorly understood. Here we describe th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.7.2298-2305.2001
更新日期:2001-04-01 00:00:00
abstract::Bacteria often encounter environments where nutrient availability is limited, and they must adapt accordingly. To identify Pasteurella multocida genes that are differentially expressed during nutrient limitation, we utilized whole-genome microarrays to compare levels of gene expression during growth in rich and minima...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.13.3734-3739.2002
更新日期:2002-07-01 00:00:00
abstract::In Escherichia coli, the three branched-chain amino acid activating enzymes appear to be essential for multivalent repression of the isoleucine- and valine-forming enzymes. The results of experiments with a mutant, strain CU18, having an altered threonine deaminase, indicate that free isoleucine and some form of threo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.117.1.29-39.1974
更新日期:1974-01-01 00:00:00
abstract::Mycobacterium avium secretes iron-binding siderophores called exochelins. The exochelins from M. avium have previously been reported to have unsaturated side chains that terminate in carboxylic acid. In contrast, our data show the side chains to be both saturated and unsaturated and to terminate with either a carboxyl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.21.6394-6398.1996
更新日期:1996-11-01 00:00:00
abstract::Listeria monocytogenes is a gram-positive, psychotrophic, food-borne pathogen which is able to grow in osmotically stressful environments. Carnitine (beta-hydroxy-L-tau-N-trimethyl aminobutyrate) can contribute significantly to growth of L. monocytogenes at high osmolarity (R. R. Beumer, M. C. te Giffel, L. J. Cox, F....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.11.3205-3212.1995
更新日期:1995-06-01 00:00:00
abstract::The citrate transporter CitP of lactic acid bacteria catalyzes electrogenic precursor-product exchange of citrate versus L-lactate during citrate-glucose cometabolism. In the absence of sugar, L-lactate is replaced by the metabolic intermediates/end products pyruvate, α-acetolactate, and acetate. In this study, the bi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00196-12
更新日期:2012-07-01 00:00:00
abstract::The addition of 5',5',5'-trifluoroleucine (fluoroleucine) to leucine auxotrophs of Salmonella typhimurium permitted protein but not ribonucleic acid (RNA) synthesis to continue after leucine depletion. The uncoupling of the formation of these macromolecules by fluoroleucine was apparent if RNA and protein synthesis wa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.99.1.107-112.1969
更新日期:1969-07-01 00:00:00
abstract::Nicotinamide adenine dinucleotide (NAD) and reduced NAD (NADH) levels have been measured in bacterial cultures. The cofactors were assayed by using the very sensitive cycling assay described previously by Cartier. Control experiments showed that the level of total NAD(H) falls during harvesting, and so samples were ta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.1.24-32.1972
更新日期:1972-07-01 00:00:00