Abstract:
:Listeria monocytogenes is a gram-positive, psychotrophic, food-borne pathogen which is able to grow in osmotically stressful environments. Carnitine (beta-hydroxy-L-tau-N-trimethyl aminobutyrate) can contribute significantly to growth of L. monocytogenes at high osmolarity (R. R. Beumer, M. C. te Giffel, L. J. Cox, F. M. Rombouts, and T. Abee, Appl. Environ. Microbiol. 60:1359-1363, 1994). Transport of L-[N-methyl-14C]carnitine in L. monocytogenes was shown to be energy dependent. Analysis of cell extracts revealed that L-carnitine was not further metabolized, which supplies evidence for its role as an osmoprotectant in L. monocytogenes. Uptake of L-carnitine proceeds in the absence of a proton motive force and is strongly inhibited in the presence of the phosphate analogs vanadate and arsenate. The L-carnitine permease is therefore most likely driven by ATP. Kinetic analysis of L-carnitine transport in glucose-energized cells revealed the presence of a high-affinity uptake system with a Km of 10 microM and a maximum rate of transport (Vmax) of 48 nmol min-1 mg of protein-1. L-[14C]carnitine transport in L. monocytogenes is significantly inhibited by a 10-fold excess of unlabelled L-carnitine, acetylcarnitine, and tau-butyrobetaine, whereas L-proline and betaine display, even at a 100-fold excess, only a weak inhibitory effect. In conclusion, an ATP-dependent L-carnitine transport system in L. monocytogenes is described, and its possible roles in cold adaptation and intracellular growth in mammalian cells are discussed.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Verheul A,Rombouts FM,Beumer RR,Abee Tdoi
10.1128/jb.177.11.3205-3212.1995subject
Has Abstractpub_date
1995-06-01 00:00:00pages
3205-12issue
11eissn
0021-9193issn
1098-5530journal_volume
177pub_type
杂志文章abstract::In bacteria, a subset of membrane proteins insert into the membrane via the Sec apparatus with the assistance of the widely conserved essential membrane protein insertase YidC. After threading into the SecYEG translocon, transmembrane segments of nascent proteins are thought to exit the translocon via a lateral gate i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01095-13
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abstract::The messenger ribonucleic acid (mRNA) pattern of log-phase and sporulating cells of Bacillus subtilis has been analyzed by deoxyribonucleic acid (DNA)-RNA hybrid studies with the complementary-strand fragments of DNA. Approximately 80% of the mRNA of log-phase and sporulating cells from stages I, III, and IV hybridize...
journal_title:Journal of bacteriology
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doi:10.1128/JB.117.2.775-782.1974
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doi:10.1128/jb.182.13.3870-3873.2000
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.13.4319-4327.1997
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.8.2469-2470.1996
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pub_type: 杂志文章
doi:10.1128/jb.175.9.2541-2551.1993
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journal_title:Journal of bacteriology
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doi:10.1128/JB.188.5.1979-1986.2006
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.11.3397-3403.1997
更新日期:1997-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.102.2.334-340.1970
更新日期:1970-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.20.6438-6445.1991
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.3.1386-1394.1979
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.1.538-539.1980
更新日期:1980-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.8.2501-2510.1992
更新日期:1992-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.5.1694-1697.1992
更新日期:1992-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
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doi:10.1128/JB.125.1.142-148.1976
更新日期:1976-01-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.164.1.270-275.1985
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.14.4059-4065.1995
更新日期:1995-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.12.5539-5544.1988
更新日期:1988-12-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.3.1340-1345.1989
更新日期:1989-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2012-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2018-08-10 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.7.2374-2378.2003
更新日期:2003-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.5.1580-1586.1968
更新日期:1968-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.117.2.631-640.1974
更新日期:1974-02-01 00:00:00