Abstract:
:The protein compositions of the membrane preparations from Selenomonas ruminantium grown in glucose or lactate medium were determined by sodium dodecyl sulfate- and two-dimensional (first, isoelectric focusing; second, sodium dodecyl sulfate) polyacrylamide slab gel electrophoresis. The outer membrane from both glucose- and lactate-grown cells contained two major proteins with apparent molecular weights of 42,000 and 40,000. These proteins existed as peptidoglycan-associated proteins in the outer membrane. The critical temperature at which they were dissociated completely into the monomeric subunits of 42,000 and 40,000 daltons was found to be 85 degrees C. The amount of each protein varied considerably depending upon the cultural conditions. The absence of the lipoprotein of Braun in S. ruminantium was suggested in our preceding paper (Y. Kamio, and H. Takahashi, J. Bacteriol. 141:888--898, 1980), and the possible absence of the protein components corresponding to the Braun lipoprotein in this strain was confirmed by electrophoretic analysis of the outer membrane and the lysozyme-treated peptidoglycan fractions. Examination of the cell surface of S. ruminantium by electron microscopy showed that the outer membrane formed a wrinkled surface with irregular blebs, some of which pinched off forming vesicles of various sizes. Rapid cell lysis occurred with the addition of a low level of lysozyme to the cell suspension. These findings led us to conclude that the physiological and morphological properties of this strain were similar to those of "deep rough" and mlp or lpo mutants of Escherichia coli K-12, respectively.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Kamio Y,Takahashi Hdoi
10.1128/JB.141.2.899-907.1980subject
Has Abstractpub_date
1980-02-01 00:00:00pages
899-907issue
2eissn
0021-9193issn
1098-5530journal_volume
141pub_type
杂志文章abstract::An 8,883-bp mini-pXO1 plasmid containing a replicon from Bacillus anthracis pXO1 (181.6 kb) was identified by making large deletions in the original plasmid using a newly developed Cre-loxP system. Portions of the truncated mini-pXO1 were cloned into an Escherichia coli vector unable to replicate in B. anthracis. A 5....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00422-09
更新日期:2009-08-01 00:00:00
abstract::Enterococcus faecalis is equipped with two pathways of glycerol dissimilation. Glycerol can either first be phosphorylated by glycerol kinase and then oxidized by glycerol-3-phosphate oxidase (the glpK pathway) or first be oxidized by glycerol dehydrogenase and then phosphorylated by dihydroxyacetone kinase (the dhaK ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00959-09
更新日期:2010-02-01 00:00:00
abstract::Comprehensive analysis of genome-wide expression patterns during growth of the hyperthermophilic bacterium Thermotoga maritima on 14 monosaccharide and polysaccharide substrates was undertaken with the goal of proposing carbohydrate specificities for transport systems and putative transcriptional regulators. Saccharid...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.21.7267-7282.2005
更新日期:2005-11-01 00:00:00
abstract::Agrobacterium tumefaciens A281 induces large, rapidly appearing tumors on a variety of plants and has a wider host range than other strains of A. tumefaciens. By using Tn3HoHo1 transposon mutagenesis and complementation analysis, a 2.5-kilobase DNA fragment which is responsible for the supervirulence phenotype was ide...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.10.4417-4425.1987
更新日期:1987-10-01 00:00:00
abstract::While studying a set of multiply marked mutants of Staphylococcus aureus strain 8325 by transformation, several instances of apparent genetic linkage were encountered. After showing that these linked transformations were readily inactivated by shearing of the deoxyribonucleic acid (DNA) but were resistant to dilution ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.1.201-211.1975
更新日期:1975-10-01 00:00:00
abstract::Expression of toxT, the transcription activator of cholera toxin and pilus production in Vibrio cholerae, is the consequence of a complex cascade of regulatory events that culminates in activation of the toxT promoter by TcpP and ToxR, two membrane-localized transcription factors. Both are encoded in operons with gene...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.24.8309-8316.2004
更新日期:2004-12-01 00:00:00
abstract::Phase variation is important in bacterial pathogenesis, since it generates antigenic variation for the evasion of immune responses and provides a strategy for quick adaptation to environmental changes. In this study, a Helicobacter pylori clone, designated MOD525, was identified that displayed phase-variable lacZ expr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.23.6615-6624.2002
更新日期:2002-12-01 00:00:00
abstract::Two cell shape-determining genes of Escherichia coli K-12, pbpA, the structural gene for penicillin-binding protein 2, and rodA, whose protein is unknown, were subcloned into plasmid vectors from the transducing phage lambda MAd lip24, which carries the lip-leuS region of the E. coli chromosome. Plasmids with restrict...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.1.10-16.1983
更新日期:1983-04-01 00:00:00
abstract::Caulobacter is a distinctive genus of prosthecate bacteria. Because caulobacters adhere to surfaces and are found in diverse locales, their role in oligotrophic environments and bacterial biofilm communities is of interest. The phylogenetic relationships of a group of marine and freshwater caulobacters were examined i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.7.2193-2198.1992
更新日期:1992-04-01 00:00:00
abstract::De Repentigny, J. (University of Montreal, Montreal, Quebec, Canada), S. Sonea, and A. Frappier. Differentiation by immunodiffusion and by quantitative immunofluorescence between 5-fluorouracil-treated and normal cells from a toxinogenic Staphylococcus aureus strain. J. Bacteriol. 88:444-448. 1964.-Immunodiffusion and...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.2.444-448.1964
更新日期:1964-08-01 00:00:00
abstract::We have isolated the Bradyrhizobium japonicum gene encoding glutamine synthetase I (glnA) from a phage lambda library by using a fragment of the Escherichia coli glnA gene as a hybridization probe. The rhizobial glnA gene has homology to the E. coli glnA gene throughout the entire length of the gene and can complement...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.2.698-703.1985
更新日期:1985-05-01 00:00:00
abstract::Four chloramphenicol resistance (Cm) and four tetracycline resistance (Tc) plasmids from Staphylococcus aureus were characterized by restriction endonuclease mapping. All four Tc plasmids had molecular masses of 2.9 megadaltons (Mdaltons) and indistinguishable responses to seven different restriction endonucleases. Th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.1.402-413.1978
更新日期:1978-10-01 00:00:00
abstract::The enzymes LinB(UT) and LinB(MI) (LinB from Sphingobium japonicum UT26 and Sphingobium sp. MI1205, respectively) catalyze the hydrolytic dechlorination of β-hexachlorocyclohexane (β-HCH) and yield different products, 2,3,4,5,6-pentachlorocyclohexanol (PCHL) and 2,3,5,6-tetrachlorocyclohexane-1,4-diol (TCDL), respecti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02020-12
更新日期:2013-06-01 00:00:00
abstract::In Escherichia coli, rotation of the flagellar motor has been shown to depend upon electrostatic interactions between charged residues of the stator protein MotA and the rotor protein FliG. These charged residues are conserved in the Na+-driven polar flagellum of Vibrio alginolyticus, but mutational studies in V. algi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.4.1466-1472.2006
更新日期:2006-02-01 00:00:00
abstract::Analysis of two virulence mutants of Pseudomonas syringae B728a revealed that the Tn 5 sites of insertion were within the gidA open reading frame (ORF). These mutations were pleiotropic, affecting diverse phenotypic traits, such as lipodepsipeptide (syringomycin and syringopeptin) antibiotic production, swarming, pres...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.8.2281-2286.2002
更新日期:2002-04-01 00:00:00
abstract::Fruiting body formation in Myxococcus xanthus involves the aggregation of cells to form mounds and the differentiation of rod-shaped cells into spherical myxospores. The surface of the myxospore is composed of several sodium dodecyl sulfate (SDS)-soluble proteins, the best characterized of which is protein S (Mr, 19,0...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.6.2141-2145.1991
更新日期:1991-03-01 00:00:00
abstract::GTP cyclohydrolase II catalyzes the first committed step in the biosynthesis of riboflavin. The gene coding for this enzyme in Escherichia coli has been cloned by marker rescue. Sequencing indicated an open reading frame of 588 bp coding for a 21.8-kDa peptide of 196 amino acids. The gene was mapped to a position at 2...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.13.4045-4051.1993
更新日期:1993-07-01 00:00:00
abstract::Inhibition of deoxyribonucleic acid (DNA) synthesis in Escherichia coli by chemical inhibitors or by shifting cultures of temperature-sensitive elongation (dnaE and dnaB) or initiation (dnaA) mutants to nonpermissive conditions led to greatly increased synthesis of the enzyme ribonucleoside diphosphate reductase, whic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.1.107-113.1977
更新日期:1977-04-01 00:00:00
abstract::Cellular vanadium metabolism was studied in Saccharomyces cerevisiae by isolating and characterizing vanadate [VO4(3-), V(V)]-resistant mutants. Vanadate growth inhibition was reversed by the removal of the vanadate from the medium, and vanadate resistance was found to be a recessive trait. Vanadate-resistant mutants ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.2.611-617.1985
更新日期:1985-11-01 00:00:00
abstract::The pdxK and pdxY genes have been found to code for pyridoxal kinases, enzymes involved in the pyridoxal phosphate salvage pathway. Two pyridoxal kinase structures have recently been published, including Escherichia coli pyridoxal kinase 2 (ePL kinase 2) and sheep pyridoxal kinase, products of the pdxY and pdxK genes,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00122-06
更新日期:2006-06-01 00:00:00
abstract::Ralston, D. J. (University of California, Berkeley) and M. D. Perry. Inhibitory action of phage K on staphylococcal dehydrogenases. II. Its possible relationship to sensitization and cell lysis. J. Bacteriol. 86:740-748. 1963.-By measuring the reduction of the dye triphenyl tetrazolium chloride to the insoluble red fo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.4.740-748.1963
更新日期:1963-10-01 00:00:00
abstract::Listeria monocytogenes is able to efficiently utilize glycerol as a carbon source. In a defined minimal medium, the growth rate (during balanced growth) in the presence of glycerol is similar to that in the presence of glucose or cellobiose. Comparative transcriptome analyses of L. monocytogenes showed high-level tran...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00259-08
更新日期:2008-08-01 00:00:00
abstract::The expression of cell cycle events in Caulobacter crescentus CB13 has been shown to be associated with regulation of carbohydrate utilization. Growth on lactose and galactose depends on induction of specific enzymes. Prior growth on glucose results in a delay in enzyme expression and cell cycle arrest at the nonmotil...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.131.3.951-959.1977
更新日期:1977-09-01 00:00:00
abstract::An NADPH-specific disulfide reductase that is active with bis-gamma-glutamylcystine has been purified 1,900-fold from Halobacterium halobium to yield a homogeneous preparation of the enzyme. Purification of this novel reductase, designated bis-gamma-glutamylcystine reductase (GCR), and purification of halobacterial di...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.8.3459-3467.1988
更新日期:1988-08-01 00:00:00
abstract::A mutant sensitive to colicin M at 30 degrees C and tolerant at 42 degrees C to high concentrations of colicin M was isolated from Escherichia coli K-12. A temperature shift from 30 to 42 degrees C rescued all cells up to the time they started to lyse at 30 degrees C (25 min after addition of colicin M). The growth ra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.147.1.135-139.1981
更新日期:1981-07-01 00:00:00
abstract::It has been reported that the accumulation of K+ by Streptococcus faecalis is mediated by a transport system which required both ATP and the proton motive force (Bakker and Harold, J. Biol. Chem. 255:433-440, 1980). My results indicate that S. faecalis has a second transport system for K+. The features of this system ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.2.506-511.1982
更新日期:1982-05-01 00:00:00
abstract::Campylobacter jejuni 81-176 lipooligosaccharide (LOS) is composed of two covalently linked domains: lipid A, a hydrophobic anchor, and a nonrepeating core oligosaccharide, consisting of an inner and outer core region. We report the isolation and characterization of the deepest rough C. jejuni 81-176 mutant by insertio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.9.3273-3279.2006
更新日期:2006-05-01 00:00:00
abstract::Gundersen, Wenche B. (Oslo University, Oslo, Norway). New type of streptomycin resistance resulting from action of the episomelike mutator factor in Escherichia coli. J. Bacteriol. 86:510-516. 1963.-Analyses have been performed to elucidate the genetic nature of the streptomycin resistance that results from the action...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.3.510-516.1963
更新日期:1963-09-01 00:00:00
abstract::The flavodoxins constitute a highly conserved family of small, acidic electron transfer proteins with flavin mononucleotide prosthetic groups. They are found in prokaryotes and in red and green algae, where they provide electrons at low potentials for the reduction of nitrogen by nitrogenase, for the light-dependent r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.5.1729-1737.1991
更新日期:1991-03-01 00:00:00
abstract::The gene for beta-amylase was isolated from Bacillus polymyxa by molecular cloning in B. subtilis. B. subtilis cells containing this gene express and secrete an amylase which resembles the B. polymyxa beta-amylase and barley beta-amylase in terms of the products it generates during carbohydrate hydrolysis. Starch hydr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.165.3.819-824.1986
更新日期:1986-03-01 00:00:00