Abstract:
:GTP cyclohydrolase II catalyzes the first committed step in the biosynthesis of riboflavin. The gene coding for this enzyme in Escherichia coli has been cloned by marker rescue. Sequencing indicated an open reading frame of 588 bp coding for a 21.8-kDa peptide of 196 amino acids. The gene was mapped to a position at 28.2 min on the E. coli chromosome and is identical with ribA. GTP cyclohydrolase II was overexpressed in a recombinant strain carrying a plasmid with the cloned gene. The enzyme was purified to homogeneity from the recombinant strain. The N-terminal sequence determined by Edman degradation was identical to the predicted sequence. The sequence is homologous to the 3' part of the central open reading frame in the riboflavin operon of Bacillus subtilis.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Richter G,Ritz H,Katzenmeier G,Volk R,Kohnle A,Lottspeich F,Allendorf D,Bacher Adoi
10.1128/jb.175.13.4045-4051.1993subject
Has Abstractpub_date
1993-07-01 00:00:00pages
4045-51issue
13eissn
0021-9193issn
1098-5530journal_volume
175pub_type
杂志文章abstract::Strains derived from HfrH carrying the arcA2 null mutation exhibit a higher respiratory rate, enhanced glucose consumption, and a more-reduced intracellular redox state than arcA deletion mutants of a different lineage. The phenotype of the arcA2 mutants was due to the presence of a creC constitutive mutation introduc...
journal_title:Journal of bacteriology
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abstract::The molecular basis of the ability of bacteria to live on caffeine via the C-8 oxidation pathway is unknown. The first step of this pathway, caffeine to trimethyluric acid (TMU), has been attributed to poorly characterized caffeine oxidases and a novel quinone-dependent caffeine dehydrogenase. Here, we report the deta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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abstract::Diazouracil (DZU) was shown to increase the mean cell volume of several bacterial species. The filaments which it induced with Escherichia coli B and Lactobacillus casei were examined in section by electron microscopy. In addition to the inhibition of division of E. coli by DZU, its effects were studied on mass increa...
journal_title:Journal of bacteriology
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doi:10.1128/JB.97.1.416-425.1969
更新日期:1969-01-01 00:00:00
abstract::Myxococcus xanthus multicellular fruiting body development is initiated by nutrient limitation at high cell density. Five clustered point mutations (sasB5, -14, -15, -16, and -17) can bypass the starvation and high-cell-density requirements for expression of the 4521 developmental reporter gene. These mutants express ...
journal_title:Journal of bacteriology
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abstract::A fusion constructed between the putative attenuator plus the first 219 nucleotides of the ermC (erythromycin resistance) structural gene and a 5' terminally deleted lacZ gene produced a moderate, basal level of beta-galactosidase which was increased by erythromycin addition. Another construction containing an intact ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.1.381-384.1984
更新日期:1984-07-01 00:00:00
abstract::RpoS is a conserved alternative sigma factor that regulates the expression of many stress response genes in Escherichia coli. The RpoS regulon is large but has not yet been completely characterized. In this study, we report the identification of over 100 RpoS-dependent fusions in a genetic screen based on the differen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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abstract::Bacillus subtilis contains three Fur homologs: Fur, PerR, and Zur. Despite significant sequence similarities, they respond to different stimuli and regulate different sets of genes. DNA target site comparisons indicate that all three paralogs recognize operators with a core 7-1-7 inverted repeat. The corresponding con...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.16.5068-5074.1999
更新日期:1999-08-01 00:00:00
abstract::In Salmonella enterica serovar Typhimurium, the stationary-phase sigma factor sigma(S) (RpoS) is required for virulence, stress resistance, biofilm formation, and development of the rdar morphotype. This morphotype is a multicellular behavior characterized by expression of the adhesive extracellular matrix components ...
journal_title:Journal of bacteriology
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更新日期:2006-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.116.2.577-581.1973
更新日期:1973-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2007-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.3.597-603.1993
更新日期:1993-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2013-06-01 00:00:00
abstract::Candida albicans is an opportunistic pathogenic yeast which frequently develops resistance to the antifungal agent fluconazole (FCZ) in patients undergoing long-term therapy. FCZ-resistant strains often display a reduced intracellular FCZ accumulation which correlates with the overexpression of the ATP-binding cassett...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.3.700-708.1999
更新日期:1999-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1991-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.19.5085-5093.1998
更新日期:1998-10-01 00:00:00
abstract::SugE of Escherichia coli, first identified as a suppressor of groEL mutations but a member of the small multidrug resistance family, has not previously been shown to confer a drug resistance phenotype. We show that high-level expression of sugE leads to resistance to a subset of toxic quaternary ammonium compounds. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.9.2543-2545.2002
更新日期:2002-05-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1969-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.1.264-267.1979
更新日期:1979-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2001-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.1.360-362.1970
更新日期:1970-10-01 00:00:00
abstract::A group of enzymes were prepared from the culture fluids of streptococci belonging to groups A, B, C, G, and L, and from a strain of Streptococcus sanguis. These streptococcal enzymes (designated St-sialidases) released a substance shown to belong to the sialic acid group from the specific substrate BSM-St, a sialomuc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.3.1328-1333.1969
更新日期:1969-03-01 00:00:00
abstract::Most mutations in the yajC-secDF operon identified via genetic screens confer a cold-sensitive growth phenotype. Here we report that two of these mutations confer this cold-sensitive phenotype by inactivating the SecDF-YajC complex in protein translocation. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2005-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2008-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.11.6187-6196.1989
更新日期:1989-11-01 00:00:00
abstract::Dormant spores of Bacillus subtilis exhibit two prominent X-ray scattering peaks. These peaks persisted in spores lacking most alpha/beta-type small, acid-soluble protein or the CotE protein responsible for assembly of much spore coat protein, but they were absent from spores of strains lacking the late sporulation-sp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2009-12-01 00:00:00
abstract::Genetic and segregation analysis of Escherichia coli strains containing a partial duplication of the trp operon reveal that the 2.5-min-long region trpD-purB is duplicated in tandem in the chromosome. The adjacent loci cysB and fabD are not duplicated. Although one copy of the duplicated region is longer than the maxi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.2.650-660.1978
更新日期:1978-02-01 00:00:00