ClpX inhibits FtsZ assembly in a manner that does not require its ATP hydrolysis-dependent chaperone activity.


:ClpX is a well-characterized bacterial chaperone that plays a role in many processes, including protein turnover and the remodeling of macromolecular complexes. All of these activities require ATP hydrolysis-dependent, ClpX-mediated protein unfolding. Here we used site-directed mutagenesis in combination with genetics and biochemistry to establish that ClpX inhibits assembly of the conserved division protein FtsZ through a noncanonical mechanism independent of its role as an ATP-dependent chaperone.


J Bacteriol


Journal of bacteriology


Haeusser DP,Lee AH,Weart RB,Levin PA




Has Abstract


2009-03-01 00:00:00














  • Occurrence of pili on and adhesive properties of Haemophilus parainfluenzae.

    abstract::Of 20 clinical isolates of Haemophilus parainfluenzae, 13 produced a mannose-resistant hemagglutinin that agglutinated erythrocytes from chickens, horses, rabbits, and sheep. Examination with the electron microscope showed that only strain HR-885 was pilate. Grown in static liquid culture, the 12 hemagglutinating, non...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kahn ME,Gromkova R

    更新日期:1981-02-01 00:00:00

  • Genetic and physical characteristics of an enterotoxin plasmid.

    abstract::We are engaged in the genetic and physical characterization of an enterotoxin (Ent) plasmid, Ent P307, which contains genes for the production of a hear-labile and a heat-stable enterotoxin. We are using an Escherichia coli K-12 strain, 711 (P307), constructed by S. Falkow, which contains no other plasmids besides Ent...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Santos DS,Palchaudhuri S,Maas WK

    更新日期:1975-12-01 00:00:00

  • Starvation-induced expression of retron-Ec107 and the role of ppGpp in multicopy single-stranded DNA production.

    abstract::Multicopy single-stranded DNA is found as a small single-stranded RNA-DNA complex in certain wild-type strains of Escherichia coli as well as in other gram-negative bacteria. Using the promoter region of the previously characterized retron-Ec107 from E. coli ECOR70, I constructed a chromosomally located lacZ operon fu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Herzer PJ

    更新日期:1996-08-01 00:00:00

  • Duplication of a 2,4-dichlorophenoxyacetic acid monooxygenase gene in Alcaligenes eutrophus JMP134(pJP4).

    abstract::The Alcaligenes eutrophus JMP134 plasmid pJP4 contains genes necessary for the complete degradation of 2,4-dichlorophenoxyacetic acid (2,4-D) and 3-chlorobenzoic acid. tfdA encodes 2,4-D monooxygenase, the initial enzyme in the 2,4-D catabolic pathway. The tfdA locus has recently been localized to a region on pJP4 13 ...

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    pub_type: 杂志文章


    authors: Perkins EJ,Lurquin PF

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  • Change in direction of flagellar rotation in Escherichia coli mediated by acetate kinase.

    abstract::Strains of Escherichia coli lacking all cytoplasmic chemotaxis proteins except CheY swim smoothly under most conditions. However, they tumble when exposed to acetate. Acetate coenzyme A synthetase (EC was thought to be essential for this response. New evidence suggests that the tumbling is mediated instead by...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Dailey FE,Berg HC

    更新日期:1993-05-01 00:00:00

  • Acquisition of glucose by Rickettsia prowazekii through the nucleotide intermediate uridine 5'-diphosphoglucose.

    abstract::The ability of Rickettsia prowazekii to transport potential sources of the glucose moiety of bacterial polysaccharides was determined. Transport was determined both by filtration assays and by centrifugation through nonaqueous layers. Uridine 5'-diphosphoglucose (UDPG) was transported, whereas glucose was not transpor...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Winkler HH,Daugherty RM

    更新日期:1986-09-01 00:00:00

  • Method for restricting incorporation of radioactivity from 3 H-thymidine into deoxyribonucleic acid only during outgrowth of spores of Bacillus cereus T.

    abstract::When heat-activated spores of Bacillus cereus T (thy(-)) were germinated and grown in medium containing (3)H-thymidine, a significant amount of radioactivity was incorporated into ribonucleic acid and deoxyribonucleic acid (DNA). A method was developed to restrict the incorporation of radioactivity from (3)H-thymidine...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Rana RS,Halvorson HO

    更新日期:1972-02-01 00:00:00

  • Identification of genes encoding components of the swarmer cell flagellar motor and propeller and a sigma factor controlling differentiation of Vibrio parahaemolyticus.

    abstract::Vibrio parahaemolyticus possesses two distinct motility systems, the polar system used for swimming in liquid environments and the lateral system used for swarming over surfaces. Growth on surfaces induces swarmer cell differentiation and expression of the lateral motility system. Mutants, created by transposon mutage...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: McCarter LL,Wright ME

    更新日期:1993-06-01 00:00:00

  • Dependence of nitrogenase switch-off upon oxygen stress on the nitrogenase activity in Azotobacter vinelandii.

    abstract::Azotobacter vinelandii was grown diazotrophically in chemostat cultures limited by sucrose, citrate, or acetate. Specific activities of cellular oxygen consumption (qO2) and nitrogenase (acetylene reduction) were measured in situ at different dilution rates (D, representing the specific growth rate mu at steady state)...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kuhla J,Oelze J

    更新日期:1988-11-01 00:00:00

  • Suppression of conformation-compromised mutants of Salmonella enterica serovar Typhimurium MelB.

    abstract::The crystal structure of the Na(+)-coupled melibiose permease of Salmonella enterica serovar Typhimurium (MelBSt) demonstrates that MelB is a member of the major facilitator superfamily of transporters. Arg residues at positions 295, 141, and 363 are involved in interdomain interactions at the cytoplasmic side by gove...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Amin A,Ethayathulla AS,Guan L

    更新日期:2014-09-01 00:00:00

  • Multiple forms of urease in cytoplasmic fractions of Ureaplasma urealyticum.

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Delisle GJ

    更新日期:1977-06-01 00:00:00

  • Identification and functional verification of archaeal-type phosphoenolpyruvate carboxylase, a missing link in archaeal central carbohydrate metabolism.

    abstract::Despite the fact that phosphoenolpyruvate carboxylase (PEPC) activity has been measured and in some cases even purified from some Archaea, the gene responsible for this activity has not been elucidated. Using sensitive sequence comparison methods, we detected a highly conserved, uncharacterized archaeal gene family th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ettema TJ,Makarova KS,Jellema GL,Gierman HJ,Koonin EV,Huynen MA,de Vos WM,van der Oost J

    更新日期:2004-11-01 00:00:00

  • Sortase-catalyzed assembly of distinct heteromeric fimbriae in Actinomyces naeslundii.

    abstract::Two types of adhesive fimbriae are expressed by Actinomyces; however, the architecture and the mechanism of assembly of these structures remain poorly understood. In this study we characterized two fimbrial gene clusters present in the genome of Actinomyces naeslundii strain MG-1. By using immunoelectron microscopy an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Mishra A,Das A,Cisar JO,Ton-That H

    更新日期:2007-04-01 00:00:00

  • Isolation of subunits from Methanosarcina barkeri ATPase: nucleotide-binding site in the alpha subunit.

    abstract::The alpha (62,000-dalton) and beta (49,000-dalton) subunits of Methanosarcina barkeri ATPase were purified to homogeneity. The subunits and ATPase complex were trypsinized in the presence of various nucleotides. ATP and ADP changed the trypsin sensitivity of the alpha subunit in the complex and isolated forms, suggest...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Inatomi K,Maeda M

    更新日期:1988-12-01 00:00:00

  • Control of EpsE, the phosphoglycosyltransferase initiating exopolysaccharide synthesis in Streptococcus thermophilus, by EpsD tyrosine kinase.

    abstract::We studied the roles of Streptococcus thermophilus phosphogalactosyltransferase (EpsE) (the priming enzyme), tyrosine kinase (EpsD), phosphatase (EpsB), and a membrane-associated protein with no known biochemical function (EpsC) in exopolysaccharide (EPS) synthesis. These proteins are well-conserved among bacteria and...

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    pub_type: 杂志文章


    authors: Minic Z,Marie C,Delorme C,Faurie JM,Mercier G,Ehrlich D,Renault P

    更新日期:2007-02-01 00:00:00

  • Identification and expression of genes narL and narX of the nar (nitrate reductase) locus in Escherichia coli K-12.

    abstract::Previous studies have shown that narL+ is required for nitrate induction of nitrate reductase synthesis and for nitrate inhibition of fumarate reductase synthesis in Escherichia coli. We cloned narL on a 5.1-kilobase HindIII fragment. Our clone also contained a previously unidentified gene, which we propose to designa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Stewart V,Parales J Jr

    更新日期:1988-04-01 00:00:00

  • Extracellular hemolysins of aerobic sporogenic bacilli.

    abstract::Forty-five strains, representing 18 species of the genus Bacillus, were surveyed for production of hemolysin against rabbit erythrocytes. Broth cultures of B. cereus, B. alvei, and B. laterosporus contained lysins that closely resembled streptolysin O. B. subtilis and a single strain of B. cereus may produce lysins ha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Bernheimer AW,Grushoff P

    更新日期:1967-05-01 00:00:00

  • Ribonucleic acid regulation in premeabilized cells of Escherichia coli capable of ribonucleic acid and protein synthesis.

    abstract::A cell permeabilization procedure is described that reduces viability less than 10% and does not significantly reduce the rates of ribonucleic acid and protein synthesis when appropriately supplemented. Permeabilization abolishes the normal stringent coupling of protein and ribonucleic acid synthesis. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Atherly AG

    更新日期:1974-06-01 00:00:00

  • Denitrification by Alcaligenes eutrophus is plasmid dependent.

    abstract::Curing of the hydrogenase-specifying megaplasmid pHG indigenous to strains of the facultative lithoautotrophic bacterium Alcaligenes eutrophus was correlated with a loss of denitrifying ability (Nitd). The retransfer of plasmid pHG1 reconstituted the Nitd phenotype. Plasmid-free mutants were still capable of convertin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Römermann D,Friedrich B

    更新日期:1985-05-01 00:00:00

  • Genetic and Proteomic Analyses of Pupylation in Streptomyces coelicolor.

    abstract:UNLABELLED:Pupylation is a posttranslational modification peculiar to actinobacteria wherein proteins are covalently modified with a small protein called the prokaryotic ubiquitin-like protein (Pup). Like ubiquitination in eukaryotes, this phenomenon has been associated with proteasome-mediated protein degradation in m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Compton CL,Fernandopulle MS,Nagari RT,Sello JK

    更新日期:2015-09-01 00:00:00

  • Genetic composition of the Bacillus subtilis SOS system.

    abstract::The SOS response in bacteria includes a global transcriptional response to DNA damage. DNA damage is sensed by the highly conserved recombination protein RecA, which facilitates inactivation of the transcriptional repressor LexA. Inactivation of LexA causes induction (derepression) of genes of the LexA regulon, many o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Au N,Kuester-Schoeck E,Mandava V,Bothwell LE,Canny SP,Chachu K,Colavito SA,Fuller SN,Groban ES,Hensley LA,O'Brien TC,Shah A,Tierney JT,Tomm LL,O'Gara TM,Goranov AI,Grossman AD,Lovett CM

    更新日期:2005-11-01 00:00:00

  • Cloning of the C-terminal cytoplasmic fragment of the tar protein and effects of the fragment on chemotaxis of Escherichia coli.

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Oosawa K,Mutoh N,Simon MI

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  • Inhibition of RNA synthesis by oxolinic acid is unrelated to average DNA supercoiling.

    abstract::Oxolinic acid reduced RNA synthesis rates whether chromosome supercoiling decreased, increased, or remained unchanged. Thus, inhibition of RNA synthesis by oxolinic acid appears to involve factors other than average DNA supercoiling level. Coumermycin A1 caused RNA synthesis rates to increase or decrease roughly in pa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Manes SH,Pruss GJ,Drlica K

    更新日期:1983-07-01 00:00:00

  • Depletion of glycolytic intermediates plays a key role in glucose-phosphate stress in Escherichia coli.

    abstract::In bacteria like Escherichia coli, the accumulation of glucose-6-phosphate (G6P) or its analogs such as α-methyl glucoside-6-phosphate (αMG6P) results in stress that appears in the form of growth inhibition. The small RNA SgrS is an essential part of the response that helps E. coli combat glucose-phosphate stress; the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Richards GR,Patel MV,Lloyd CR,Vanderpool CK

    更新日期:2013-11-01 00:00:00

  • Relative Rates of Amino Acid Import via the ABC Transporter GlnPQ Determine the Growth Performance of Lactococcus lactis.

    abstract:UNLABELLED:The GlnPQ transporter from Lactococcus lactis has the remarkable feature of having two substrate-binding domains (SBDs) fused to the N terminus of the transmembrane domain (TMD), and thus four SBDs are present in the homodimeric complex. Although X-ray structures and ligand binding data are available for bot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Fulyani F,Schuurman-Wolters GK,Slotboom DJ,Poolman B

    更新日期:2015-11-09 00:00:00

  • Bacterial catalysis of nitrosation: involvement of the nar operon of Escherichia coli.

    abstract::We have developed a rapid and sensitive fluorimetric method, based on the formation of a fluorescent product from nitrosation of 2,3-diaminonaphthalene, for measuring the ability of bacteria to catalyze nitrosation of amines. We have shown in Escherichia coli that nitrosation can be induced under anaerobic conditions ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ralt D,Wishnok JS,Fitts R,Tannenbaum SR

    更新日期:1988-01-01 00:00:00

  • Molecular and functional characterization of a unique sucrose hydrolase from Xanthomonas axonopodis pv. glycines.

    abstract::A novel sucrose hydrolase (SUH) from Xanthomonas axonopodis pv. glycines, a causative agent of bacterial pustule disease on soybeans, was studied at the functional and molecular levels. SUH was shown to act rather specifically on sucrose (K(m) = 2.5 mM) but not on sucrose-6-phosphate. Protein analysis of purified SUH ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kim HS,Park HJ,Heu S,Jung J

    更新日期:2004-01-01 00:00:00

  • A Trigger Residue for Transmembrane Signaling in the Escherichia coli Serine Chemoreceptor.

    abstract:UNLABELLED:The transmembrane Tsr protein of Escherichia coli mediates chemotactic responses to environmental serine gradients. Serine binds to the periplasmic domain of the homodimeric Tsr molecule, promoting a small inward displacement of one transmembrane helix (TM2). TM2 piston displacements, in turn, modulate the s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kitanovic S,Ames P,Parkinson JS

    更新日期:2015-08-01 00:00:00

  • The gene for ribosomal protein S21, rpsU, maps close to dnaG at 66.5 min on the Escherichia coli chromosomal linkage map.

    abstract::A series of mutations causing alterations in ribosomal protein S21 was mapped close to dnaG, around 66.5 min on the Escherichia coli chromosomal linkage map. They cotransduced about 95% with this marker. These mutations define gene rpsU, which is very likely the structural gene for protein S21. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Dabbs ER

    更新日期:1980-11-01 00:00:00

  • Genetic mapping of katA, a locus that affects catalase 1 level in Bacillus subtilis.

    abstract::Several mutants of Bacillus subtilis deficient in catalase synthesis generated by nitrosoguanidine mutagenesis have been used to map a locus affecting catalase activity. Two- and three-factor bacteriophage PBS1 transductional crosses were used to locate the locus, named katA, between recH and thiA with 98% linkage to ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Loewen PC,Switala J

    更新日期:1987-12-01 00:00:00