Abstract:
:ClpX is a well-characterized bacterial chaperone that plays a role in many processes, including protein turnover and the remodeling of macromolecular complexes. All of these activities require ATP hydrolysis-dependent, ClpX-mediated protein unfolding. Here we used site-directed mutagenesis in combination with genetics and biochemistry to establish that ClpX inhibits assembly of the conserved division protein FtsZ through a noncanonical mechanism independent of its role as an ATP-dependent chaperone.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Haeusser DP,Lee AH,Weart RB,Levin PAdoi
10.1128/JB.01606-07subject
Has Abstractpub_date
2009-03-01 00:00:00pages
1986-91issue
6eissn
0021-9193issn
1098-5530pii
JB.01606-07journal_volume
191pub_type
杂志文章abstract::Of 20 clinical isolates of Haemophilus parainfluenzae, 13 produced a mannose-resistant hemagglutinin that agglutinated erythrocytes from chickens, horses, rabbits, and sheep. Examination with the electron microscope showed that only strain HR-885 was pilate. Grown in static liquid culture, the 12 hemagglutinating, non...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.2.1075-1078.1981
更新日期:1981-02-01 00:00:00
abstract::We are engaged in the genetic and physical characterization of an enterotoxin (Ent) plasmid, Ent P307, which contains genes for the production of a hear-labile and a heat-stable enterotoxin. We are using an Escherichia coli K-12 strain, 711 (P307), constructed by S. Falkow, which contains no other plasmids besides Ent...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.3.1240-1247.1975
更新日期:1975-12-01 00:00:00
abstract::Multicopy single-stranded DNA is found as a small single-stranded RNA-DNA complex in certain wild-type strains of Escherichia coli as well as in other gram-negative bacteria. Using the promoter region of the previously characterized retron-Ec107 from E. coli ECOR70, I constructed a chromosomally located lacZ operon fu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.15.4438-4444.1996
更新日期:1996-08-01 00:00:00
abstract::The Alcaligenes eutrophus JMP134 plasmid pJP4 contains genes necessary for the complete degradation of 2,4-dichlorophenoxyacetic acid (2,4-D) and 3-chlorobenzoic acid. tfdA encodes 2,4-D monooxygenase, the initial enzyme in the 2,4-D catabolic pathway. The tfdA locus has recently been localized to a region on pJP4 13 ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.12.5669-5672.1988
更新日期:1988-12-01 00:00:00
abstract::Strains of Escherichia coli lacking all cytoplasmic chemotaxis proteins except CheY swim smoothly under most conditions. However, they tumble when exposed to acetate. Acetate coenzyme A synthetase (EC 6.2.1.1) was thought to be essential for this response. New evidence suggests that the tumbling is mediated instead by...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.10.3236-3239.1993
更新日期:1993-05-01 00:00:00
abstract::The ability of Rickettsia prowazekii to transport potential sources of the glucose moiety of bacterial polysaccharides was determined. Transport was determined both by filtration assays and by centrifugation through nonaqueous layers. Uridine 5'-diphosphoglucose (UDPG) was transported, whereas glucose was not transpor...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.3.805-808.1986
更新日期:1986-09-01 00:00:00
abstract::When heat-activated spores of Bacillus cereus T (thy(-)) were germinated and grown in medium containing (3)H-thymidine, a significant amount of radioactivity was incorporated into ribonucleic acid and deoxyribonucleic acid (DNA). A method was developed to restrict the incorporation of radioactivity from (3)H-thymidine...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.109.2.599-605.1972
更新日期:1972-02-01 00:00:00
abstract::Vibrio parahaemolyticus possesses two distinct motility systems, the polar system used for swimming in liquid environments and the lateral system used for swarming over surfaces. Growth on surfaces induces swarmer cell differentiation and expression of the lateral motility system. Mutants, created by transposon mutage...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.11.3361-3371.1993
更新日期:1993-06-01 00:00:00
abstract::Azotobacter vinelandii was grown diazotrophically in chemostat cultures limited by sucrose, citrate, or acetate. Specific activities of cellular oxygen consumption (qO2) and nitrogenase (acetylene reduction) were measured in situ at different dilution rates (D, representing the specific growth rate mu at steady state)...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.11.5325-5329.1988
更新日期:1988-11-01 00:00:00
abstract::The crystal structure of the Na(+)-coupled melibiose permease of Salmonella enterica serovar Typhimurium (MelBSt) demonstrates that MelB is a member of the major facilitator superfamily of transporters. Arg residues at positions 295, 141, and 363 are involved in interdomain interactions at the cytoplasmic side by gove...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01868-14
更新日期:2014-09-01 00:00:00
abstract::The urease activity of Ureaplasma urealyticum was found to be located in the cytoplasmic fraction and consisted of multiple stable enzyme forms representing at least four biotypes. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.3.1390-1392.1977
更新日期:1977-06-01 00:00:00
abstract::Despite the fact that phosphoenolpyruvate carboxylase (PEPC) activity has been measured and in some cases even purified from some Archaea, the gene responsible for this activity has not been elucidated. Using sensitive sequence comparison methods, we detected a highly conserved, uncharacterized archaeal gene family th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.22.7754-7762.2004
更新日期:2004-11-01 00:00:00
abstract::Two types of adhesive fimbriae are expressed by Actinomyces; however, the architecture and the mechanism of assembly of these structures remain poorly understood. In this study we characterized two fimbrial gene clusters present in the genome of Actinomyces naeslundii strain MG-1. By using immunoelectron microscopy an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01952-06
更新日期:2007-04-01 00:00:00
abstract::The alpha (62,000-dalton) and beta (49,000-dalton) subunits of Methanosarcina barkeri ATPase were purified to homogeneity. The subunits and ATPase complex were trypsinized in the presence of various nucleotides. ATP and ADP changed the trypsin sensitivity of the alpha subunit in the complex and isolated forms, suggest...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.12.5960-5962.1988
更新日期:1988-12-01 00:00:00
abstract::We studied the roles of Streptococcus thermophilus phosphogalactosyltransferase (EpsE) (the priming enzyme), tyrosine kinase (EpsD), phosphatase (EpsB), and a membrane-associated protein with no known biochemical function (EpsC) in exopolysaccharide (EPS) synthesis. These proteins are well-conserved among bacteria and...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01122-06
更新日期:2007-02-01 00:00:00
abstract::Previous studies have shown that narL+ is required for nitrate induction of nitrate reductase synthesis and for nitrate inhibition of fumarate reductase synthesis in Escherichia coli. We cloned narL on a 5.1-kilobase HindIII fragment. Our clone also contained a previously unidentified gene, which we propose to designa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.4.1589-1597.1988
更新日期:1988-04-01 00:00:00
abstract::Forty-five strains, representing 18 species of the genus Bacillus, were surveyed for production of hemolysin against rabbit erythrocytes. Broth cultures of B. cereus, B. alvei, and B. laterosporus contained lysins that closely resembled streptolysin O. B. subtilis and a single strain of B. cereus may produce lysins ha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.5.1541-1543.1967
更新日期:1967-05-01 00:00:00
abstract::A cell permeabilization procedure is described that reduces viability less than 10% and does not significantly reduce the rates of ribonucleic acid and protein synthesis when appropriately supplemented. Permeabilization abolishes the normal stringent coupling of protein and ribonucleic acid synthesis. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.118.3.1186-1189.1974
更新日期:1974-06-01 00:00:00
abstract::Curing of the hydrogenase-specifying megaplasmid pHG indigenous to strains of the facultative lithoautotrophic bacterium Alcaligenes eutrophus was correlated with a loss of denitrifying ability (Nitd). The retransfer of plasmid pHG1 reconstituted the Nitd phenotype. Plasmid-free mutants were still capable of convertin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.2.852-854.1985
更新日期:1985-05-01 00:00:00
abstract:UNLABELLED:Pupylation is a posttranslational modification peculiar to actinobacteria wherein proteins are covalently modified with a small protein called the prokaryotic ubiquitin-like protein (Pup). Like ubiquitination in eukaryotes, this phenomenon has been associated with proteasome-mediated protein degradation in m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00302-15
更新日期:2015-09-01 00:00:00
abstract::The SOS response in bacteria includes a global transcriptional response to DNA damage. DNA damage is sensed by the highly conserved recombination protein RecA, which facilitates inactivation of the transcriptional repressor LexA. Inactivation of LexA causes induction (derepression) of genes of the LexA regulon, many o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.22.7655-7666.2005
更新日期:2005-11-01 00:00:00
abstract::A gene encoding only the C-terminal portion of the receptor-transducer protein Tar of Escherichia coli was constructed. The gene product was detected and localized in the cytoplasmic fraction of the cell by immunoblotting with anti-Tar antibodies. The C-terminal fragments from wild-type and mutant tar genes were chara...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.6.2521-2526.1988
更新日期:1988-06-01 00:00:00
abstract::Oxolinic acid reduced RNA synthesis rates whether chromosome supercoiling decreased, increased, or remained unchanged. Thus, inhibition of RNA synthesis by oxolinic acid appears to involve factors other than average DNA supercoiling level. Coumermycin A1 caused RNA synthesis rates to increase or decrease roughly in pa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.1.420-423.1983
更新日期:1983-07-01 00:00:00
abstract::In bacteria like Escherichia coli, the accumulation of glucose-6-phosphate (G6P) or its analogs such as α-methyl glucoside-6-phosphate (αMG6P) results in stress that appears in the form of growth inhibition. The small RNA SgrS is an essential part of the response that helps E. coli combat glucose-phosphate stress; the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00705-13
更新日期:2013-11-01 00:00:00
abstract:UNLABELLED:The GlnPQ transporter from Lactococcus lactis has the remarkable feature of having two substrate-binding domains (SBDs) fused to the N terminus of the transmembrane domain (TMD), and thus four SBDs are present in the homodimeric complex. Although X-ray structures and ligand binding data are available for bot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00685-15
更新日期:2015-11-09 00:00:00
abstract::We have developed a rapid and sensitive fluorimetric method, based on the formation of a fluorescent product from nitrosation of 2,3-diaminonaphthalene, for measuring the ability of bacteria to catalyze nitrosation of amines. We have shown in Escherichia coli that nitrosation can be induced under anaerobic conditions ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.1.359-364.1988
更新日期:1988-01-01 00:00:00
abstract::A novel sucrose hydrolase (SUH) from Xanthomonas axonopodis pv. glycines, a causative agent of bacterial pustule disease on soybeans, was studied at the functional and molecular levels. SUH was shown to act rather specifically on sucrose (K(m) = 2.5 mM) but not on sucrose-6-phosphate. Protein analysis of purified SUH ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.2.411-418.2004
更新日期:2004-01-01 00:00:00
abstract:UNLABELLED:The transmembrane Tsr protein of Escherichia coli mediates chemotactic responses to environmental serine gradients. Serine binds to the periplasmic domain of the homodimeric Tsr molecule, promoting a small inward displacement of one transmembrane helix (TM2). TM2 piston displacements, in turn, modulate the s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00274-15
更新日期:2015-08-01 00:00:00
abstract::A series of mutations causing alterations in ribosomal protein S21 was mapped close to dnaG, around 66.5 min on the Escherichia coli chromosomal linkage map. They cotransduced about 95% with this marker. These mutations define gene rpsU, which is very likely the structural gene for protein S21. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.2.603-607.1980
更新日期:1980-11-01 00:00:00
abstract::Several mutants of Bacillus subtilis deficient in catalase synthesis generated by nitrosoguanidine mutagenesis have been used to map a locus affecting catalase activity. Two- and three-factor bacteriophage PBS1 transductional crosses were used to locate the locus, named katA, between recH and thiA with 98% linkage to ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.12.5848-5851.1987
更新日期:1987-12-01 00:00:00