Abstract:
:In Klebsiella pneumoniae, products of the nitrogen fixation nifLA operon regulate transcription of the other nif operons. NifA activates transcription by sigma54-holoenzyme. In vivo, NifL antagonizes the action of NifA under aerobic conditions or in the presence of combined nitrogen. In contrast to a previous report, we show that depletion of iron (Fe) from the growth medium with the chelating agent o-phenanthroline (20 microM) mimics aerobiosis or combined nitrogen in giving rise to inhibition of NifA activity even under anaerobic, nitrogen-limiting conditions. Adding back Fe in only twofold molar excess over phenanthroline restores NifA activity, whereas adding other metals fails to do so. By using strains that lack NifL, we showed that NifA activity itself does not require Fe and is not directly affected by phenanthroline. Hence, Fe is required to relieve the inhibition of NifA activity by NifL in vivo. Despite the Fe requirement in vivo, we have found no evidence that NifL contains Fe or an iron-sulfur (Fe-S) cluster. Determination of the molecular mass of an inhibitory form of NifL overproduced under aerobic conditions indicated that it was not posttranslationally modified. When NifL was synthesized in vitro, it inhibited transcriptional activation by NifA even when it was synthesized under anaerobic conditions in the presence of a high Fe concentration or of superoxide dismutase, which is known to protect some Fe-S clusters. Moreover, overproduction of superoxide dismutase in vivo did not relieve NifL, inhibition under aerobic conditions, and attempts to relieve NifL inhibition in vitro by reconstituting Fe-S clusters with the NifS enzyme (Azotobacter vinelandii) were unsuccessful. Since we obtained no evidence that Fe acts directly on NifL or NifA, we postulate that an additional Fe-containing protein, not yet identified, may be required to relieve NifL inhibition under anaerobic, nitrogen-limiting conditions.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Schmitz RA,He L,Kustu Sdoi
10.1128/jb.178.15.4679-4687.1996subject
Has Abstractpub_date
1996-08-01 00:00:00pages
4679-87issue
15eissn
0021-9193issn
1098-5530journal_volume
178pub_type
杂志文章abstract::CTXphi is a filamentous, lysogenic bacteriophage whose genome encodes cholera toxin, the primary virulence factor produced by Vibrio cholerae. CTX prophages in O1 El Tor and O139 strains of V. cholerae are found within arrays of genetically related elements integrated at a single locus within the V. cholerae large chr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.24.6992-6998.2000
更新日期:2000-12-01 00:00:00
abstract::PcsB is a protein of unknown function that plays a critical role in cell division in Streptococcus pneumoniae and other ovococcus species of Streptococcus. We constructed isogenic sets of mutants expressing different amounts of PcsB in laboratory strain R6 and virulent serotype 2 strain D39 to evaluate its cellular ro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01505-08
更新日期:2009-05-01 00:00:00
abstract::Escherichia coli has a formate hydrogenlyase system which allows it to maintain an electron balance during anaerobic growth by passing electrons from formate to H+ ions, thus generating H2. The Mu d1(Ap lac) bacteriophage was used to generate mutants that were defective in passing electrons from formate to benzyl viol...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.1.180-186.1984
更新日期:1984-04-01 00:00:00
abstract::MobB is a small protein encoded by the broad-host-range plasmid R1162 and required for efficient mobilization of its DNA during conjugation. The protein was shown previously to stabilize the relaxosome, the complex of plasmid DNA and mobilization proteins at the origin of transfer (oriT). We have generated in-frame mo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.7.2124-2131.1999
更新日期:1999-04-01 00:00:00
abstract::In Escherichia coli, the frequencies of IS50 and Tn5 transposition are greater in Dam- cells than in isogenic Dam+ cells. IS50 transposition is increased approximately 1,000-fold and Tn5 transposition frequencies are increased about 5- to 10-fold in the absence of Dam methylation. However, in cells that are deficient ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.3.1368-1373.1990
更新日期:1990-03-01 00:00:00
abstract::Desulfurococcus kamchatkensis is an anaerobic organotrophic hyperthermophilic crenarchaeon isolated from a terrestrial hot spring. Its genome consists of a single circular chromosome of 1,365,223 bp with no extrachromosomal elements. A total of 1,474 protein-encoding genes were annotated, among which 205 are exclusive...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01525-08
更新日期:2009-04-01 00:00:00
abstract::Saccharomyces cerevisiae cells, grown on a fermentable carbon source, display two growth phases before they enter the stationary phase: a rapid phase (log phase) followed by a slow phase. It was previously shown that a subunit of the yeast RNA polymerase II, RPB4, positively affects the activity of the enzyme in post-...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.19.6358-6363.1993
更新日期:1993-10-01 00:00:00
abstract::In the nosocomial opportunistic pathogen Acinetobacter baumannii, RecA-dependent mutagenesis, which causes antibiotic resistance acquisition, is linked to the DNA damage response (DDR). Notably, unlike the Escherichia coli paradigm, recA and DDR gene expression in A. baumannii is bimodal. Namely, there is phenotypic v...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00799-16
更新日期:2017-05-09 00:00:00
abstract::The gene encoding Neisseria gonorrhoeae periplasmic binding protein FbpA contains two regions whose sequences exhibit homology with the Escherichia coli ferric uptake regulator protein (Fur) consensus binding sequence. In this study, DNase I footprinting experiments were employed to characterize the operator sequences...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.16.5020-5023.1996
更新日期:1996-08-01 00:00:00
abstract::Edwards, P. R. (Communicable Disease Center, U.S. Public Health Service, Atlanta, Ga.), R. Sakazaki, and I. Kato. Natural occurrence of four reversible flagellar phases in cultures of Salmonella mikawashima. J. Bacteriol. 84:99-103. 1962-Study of cultures of Salmonella mikawashima isolated from reptiles revealed that ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.1.99-103.1962
更新日期:1962-07-01 00:00:00
abstract::Coxiella burnetii, the etiological agent of acute and chronic Q fever in humans, is a naturally intracellular pathogen that directs the formation of an acidic Coxiella-containing vacuole (CCV) derived from the host lysosomal network. Central to its pathogenesis is a specialized type IVB secretion system (T4SS) that de...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00071-13
更新日期:2013-09-01 00:00:00
abstract::A DNA fragment from Xanthomonas campestris pv. campestris that partially restored the carbohydrate fermentation pattern of a cya crp Escherichia coli strain was cloned and expressed in E. coli. The nucleotide sequence of this fragment revealed the presence of a 700-base-pair open reading frame that coded for a protein...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.10.5877-5883.1990
更新日期:1990-10-01 00:00:00
abstract::When soluble extracts of the extreme acidothermophilic archaeon Sulfolobus solfataricus were incubated with [gamma-(32)P]ATP, several proteins were radiolabeled. One of the more prominent of these, which migrated with a mass of approximately 46 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.7.2112-2121.2003
更新日期:2003-04-01 00:00:00
abstract::During growth of the halophilic archaeon Haloarcula marismortui on D-xylose, a specific D-xylose dehydrogenase was induced. The enzyme was purified to homogeneity. It constitutes a homotetramer of about 175 kDa and catalyzed the oxidation of xylose with both NADP+ and NAD+ as cosubstrates with 10-fold higher affinity ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.18.6198-6207.2004
更新日期:2004-09-01 00:00:00
abstract::Streptomyces coelicolor is the prototype for the investigation of antibiotic-producing and differentiating actinomycetes. As soil bacteria, streptomycetes can metabolize a wide variety of carbon sources and are hence vested with various specific permeases. Their activity and regulation substantially determine the nutr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.5.1362-1373.2004
更新日期:2004-03-01 00:00:00
abstract::Bacteriophage lambda adsorbs to its Escherichia coli K-12 host by interacting with LamB, its cell-surface receptor. We fused C-terminal portions of J, the tail fiber protein of lambda, to maltose-binding protein. Solid-phase binding assays demonstrated that a purified fusion protein comprising only the last 249 residu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.2.508-512.2000
更新日期:2000-01-01 00:00:00
abstract::Vibrio cholerae is a motile bacterium responsible for the disease cholera, and motility has been hypothesized to be inversely regulated with virulence. We examined the transcription profiles of V. cholerae strains containing mutations in flagellar regulatory genes (rpoN, flrA, flrC, and fliA) by utilizing whole-genome...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00949-09
更新日期:2009-11-01 00:00:00
abstract::Ribulose 1,5-bisphosphate carboxylase-oxygenase (RuBPC-O) activity was greatly enhanced when Rhodopseudomonas sphaeroides was grown in a mineral salts medium supplied with 1.5% CO2 in hydrogen. Analysis of cell extracts by sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that cells growing on 1.5% C...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.165.2.620-624.1986
更新日期:1986-02-01 00:00:00
abstract::Although four of the five Salmonella pathogenicity islands (SPIs) have been characterized in detail for Salmonella enterica serovar Typhimurium, and the fifth has been characterized for Salmonella enterica serovar Dublin, there have been limited studies to examine them in detail in a range of pathogenic serovars of S....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.12.3624-3635.2003
更新日期:2003-06-01 00:00:00
abstract::An approach originally designed to identify functional origins of conjugative transfer (oriT or mob) in a bacterial genome (J. A. Herrera-Cervera, J. M. Sanjuán-Pinilla, J. Olivares, and J. Sanjuán, J. Bacteriol. 180:4583-4590, 1998) was modified to improve its reliability and prevent selection of undesired false mob ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.17.5753-5761.2004
更新日期:2004-09-01 00:00:00
abstract::A procedure to identify genes that are lethal when cloned on multicopy plasmids was developed. It depends on the ability of mini-Mu plasmid elements to be used for both in vivo cloning and generalized transduction of enterobacterial genes. The feasibility of this procedure was demonstrated by using the tetA gene of Tn...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.1.468-470.1988
更新日期:1988-01-01 00:00:00
abstract::The lethal and mutagenic effects of various mutagens on Neisseria gonorrhoeae were investigated. Lethality studies demonstrated that N. gonorrhoeae was relatively sensitive to ethyl methanesulfonate, UV light, and methyl methanesulfonate. Although N. gonorrhoeae was readily mutated by ethyl methanesulfonate and N-meth...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.1.288-293.1984
更新日期:1984-10-01 00:00:00
abstract::Motility of bacteria like Salmonella enterica is a highly regulated process that responds to a variety of internal and external stimuli. A hierarchy of three promoter classes characterizes the Salmonella flagellar system, and the onset of flagellar gene expression depends on the oligomeric regulatory complex and class...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00728-13
更新日期:2013-09-01 00:00:00
abstract::Cadmium-resistant Pseudomonas putida GAM-1, which was able to grow in concentrations of CdCl2 as high as 7 mM, was isolated from soil in a rice paddy. This bacterium harbored a DNA plasmid of about 52 kilobases. The plasmid (pGU100) transformed Escherichia coli C600 to cadmium resistance. A cadmium-resistant transform...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.165.1.334-335.1986
更新日期:1986-01-01 00:00:00
abstract::The Escherichia coli K-12 mtr gene, which encodes a tryptophan-specific permease, was cloned, and its nucleotide sequence was determined. The precise location of the mtr gene at 69 min on the E. coli chromosome was determined. The mtr gene product was identified as a 414-amino-acid residue protein with a calculated mo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.13.4133-4143.1991
更新日期:1991-07-01 00:00:00
abstract::The pED208 plasmid is a 90-kilobase conjugative plasmid which is the derepressed form of Fo lac plasmid (IncFV). A 3.3-kilobase HindIII-PstI fragment from the pED208 plasmid was cloned and sequenced and was found to contain four open reading frames which were highly homologous to the traA, traL, traE, and traY gene pr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.2.990-998.1986
更新日期:1986-11-01 00:00:00
abstract::Picolinic acid (PA) is a natural toxic pyridine derivative. Microorganisms can degrade and utilize PA for growth. However, the full catabolic pathway of PA and its physiological and genetic foundation remain unknown. In this study, we identified a gene cluster, designated picRCEDFB4B3B2B1A1A2A3, responsible for the de...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00077-19
更新日期:2019-07-24 00:00:00
abstract::Curing of the hydrogenase-specifying megaplasmid pHG indigenous to strains of the facultative lithoautotrophic bacterium Alcaligenes eutrophus was correlated with a loss of denitrifying ability (Nitd). The retransfer of plasmid pHG1 reconstituted the Nitd phenotype. Plasmid-free mutants were still capable of convertin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.2.852-854.1985
更新日期:1985-05-01 00:00:00
abstract::Purine nucleoside phosphorylase (EC 2.4.2.1) from Bacillus cereus T was examined at hourly intervals during growth and sporulation. The enzyme has maximal activity in extracts prepared from cells during stages I and II. The activity during exponential growth is only 6.6% of the maximum and that in free spores is only ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.1.33-36.1972
更新日期:1972-07-01 00:00:00
abstract::The Yersinia adhesin A (YadA) is a trimeric autotransporter adhesin of enteric yersiniae. It consists of three major domains: a head mediating adherence to host cells, a stalk involved in serum resistance, and an anchor that forms a membrane pore and is responsible for the autotransport function. The anchor contains a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00985-07
更新日期:2007-12-01 00:00:00