Abstract:
:A DNA fragment from Xanthomonas campestris pv. campestris that partially restored the carbohydrate fermentation pattern of a cya crp Escherichia coli strain was cloned and expressed in E. coli. The nucleotide sequence of this fragment revealed the presence of a 700-base-pair open reading frame that coded for a protein highly similar to the catabolite activation factor (CAP) of E. coli (accordingly named CLP for CAP-like protein). An X. campestris pv. campestris clp mutant was constructed by reverse genetics. This strain was not affected in the utilization of various carbon sources but had strongly reduced pathogenicity. Production of xanthan gum, pigment, and extracellular enzymes was either increased or decreased, suggesting that CLP plays a role in the regulation of phytopathogenicity.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
de Crecy-Lagard V,Glaser P,Lejeune P,Sismeiro O,Barber CE,Daniels MJ,Danchin Adoi
10.1128/jb.172.10.5877-5883.1990subject
Has Abstractpub_date
1990-10-01 00:00:00pages
5877-83issue
10eissn
0021-9193issn
1098-5530journal_volume
172pub_type
杂志文章abstract::Cells of the photosynthetic bacterium Rhodospirillum rubrum cultivated anaerobically in light show phototaxis. The behavior of individual cells in response to the phenomenon is reversal(s) of the swimming direction when the intensity of the light available to them abruptly decreases. The tactic response was inhibited ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.131.1.34-41.1977
更新日期:1977-07-01 00:00:00
abstract::The lytD1 mutant of Escherichia coli exhibits temperature-sensitive growth which is attributed to cellular autolysis at the restrictive temperature. Either of two cloned phage lambda genes, identified as cI and cro, suppressed the lytD1(Ts) lysis phenotype, suggesting that lytD encodes a DNA-binding protein with a DNA...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.2.893-895.1991
更新日期:1991-01-01 00:00:00
abstract::At least six phenotypically distinct classes of mutants of Escherichia coli which require serine or pyridoxine or both can be isolated. Three of the six classes lack 3-phosphoserine-2-oxoglutarate aminotransferase. One of these classes contains WG5, a mutant previously characterized as containing the pdxF5 allele. The...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.2.658-667.1970
更新日期:1970-11-01 00:00:00
abstract::Integrons are genetic elements that contribute to lateral gene transfer in bacteria as a consequence of possessing a site-specific recombination system. This system facilitates the spread of genes when they are part of mobile cassettes. Most integrons are contained within chromosomes and are confined to specific bacte...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01950-05
更新日期:2006-08-01 00:00:00
abstract::Cotransformation frequencies of 16, 39, 51, and 60% were observed when donor alleles were separated by distances of 9.2, 7.4, 6.3, and 5.1 kb, respectively, in donor Acinetobacter calcoaceticus DNA. A different and unexpected pattern was observed when the distance between recipient alleles was reduced from 9.2 to 5.1 ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.20.6015-6017.1995
更新日期:1995-10-01 00:00:00
abstract::By using a method that labels sulfhydryl-containing proteins in situ, we have detected a major outer membrane protein of Neisseria gonorrhoeae at 41 kDa. A protein of this molecular mass has not previously been shown to be a major outer membrane protein in gonococci. In addition, a minor protein rich in cysteinyl resi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.4.1173-1175.1993
更新日期:1993-02-01 00:00:00
abstract::The sigma(54)-dependent regulator NorR activates transcription of target genes in response to nitric oxide (NO) or NO-generating agents. In Ralstonia eutropha H16, NorR activates transcription of the dicistronic norAB operon that encodes NorA, a protein of unknown function, and NorB, a nitric oxide reductase. A consti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.23.7980-7987.2004
更新日期:2004-12-01 00:00:00
abstract::Overexpression of evgA, a response regulator of a two-component system, increased multidrug efflux in Escherichia coli. Since overexpression of the emrKY operon, which is controlled by evgAS, could account only for deoxycholate resistance, the evgAS locus apparently controls expression of at least one other multidrug ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.4.1455-1458.2001
更新日期:2001-02-01 00:00:00
abstract::A reaction center H- strain (RCH-) of Rhodobacter sphaeroides, PUHA1, was made by in vitro deletion of an XhoI restriction endonuclease fragment from the puhA gene coupled with insertion of a kanamycin resistance gene cartridge. The resulting construct was delivered to R. sphaeroides wild-type 2.4.1, with the defectiv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.1.436-446.1989
更新日期:1989-01-01 00:00:00
abstract::Several characteristics of Mycobacterium tuberculosis (e.g., conserved genome and low growth rate) have severely restricted the study of the microorganism. The discovery of IS6110 raised hopes of overcoming these obstacles. However, our knowledge of this IS element is relatively limited; even its two basic characteris...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.183.18.5279-5284.2001
更新日期:2001-09-01 00:00:00
abstract::The cytoplasmic accumulation of exogenous betaine stimulates the growth of Lactococcus lactis cultivated under hyperosmotic conditions. We report that L. lactis possesses a single betaine transport system that belongs to the ATP-binding cassette (ABC) superfamily of transporters. Through transposon mutagenesis, a muta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.20.6238-6246.1999
更新日期:1999-10-01 00:00:00
abstract::Under anaerobic conditions, Klebsiella pneumoniae reduced nitrite (NO2-), yielding nitrous oxide (N2O) and ammonium ions (NH4+) as products. Nitrous oxide formation accounted for about 5% of the total NO2- reduced, and NH4+ production accounted for the remainder. Glucose and pyruvate were the electron donors for NO2- ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.2.454-458.1983
更新日期:1983-08-01 00:00:00
abstract::Tetracycline accumulation by the phototrophic bacterium Rhodopseudomonas sphaeroides has been studied, using the fluorescence properties of the antibiotic and measuring uptake of [7- 3H]tetracycline. Accumulation was carrier mediated, with a Km of approximately 300 micronM. Efflux also appeared to be carried mediated,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.3.678-683.1979
更新日期:1979-06-01 00:00:00
abstract::An assay was developed to determine the amount of cyanophycin granules in blue-green algae. The amount of this polypeptide in cells of Anabaena cylindrica was measured as a function of culture age and was compared with changes in other proteinaceous cellular components. The data presented support the notion that the c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.3.1213-1216.1973
更新日期:1973-06-01 00:00:00
abstract::The Tol-Pal system of Escherichia coli is involved in maintaining outer membrane stability. Mutations in tolQ, tolR, tolA, tolB, or pal genes result in sensitivity to bile salts and the leakage of periplasmic proteins. Moreover, some of the tol genes are necessary for the entry of group A colicins and the DNA of filam...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.3.821-824.2000
更新日期:2000-02-01 00:00:00
abstract::The cytoskeletal proteins HMW1 and HMW2 are components of the terminal organelle of the cell wall-less bacterium Mycoplasma pneumoniae. HMW1 is required for a tapered, filamentous morphology but exhibits accelerated turnover in the absence of HMW2. Here, we report that a reciprocal dependency exists between HMW1 and H...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.24.8221-8228.2004
更新日期:2004-12-01 00:00:00
abstract::pGC1, a self-transmissible lac plasmid that originated in Yersinia enterocolitica 842, is described. This plasmid is freely transmissible between strains of Y. enterocolitica and from such strains to strains of Escherichia coli. The plasmid can also be transferref among G. coli strains and back to Y. enterocolitica, a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.127.3.1058-1062.1976
更新日期:1976-09-01 00:00:00
abstract::The weak organic acid sorbic acid is a commonly used food preservative, as it inhibits the growth of bacteria, yeasts, and molds. We have used genome-wide transcriptional profiling of Bacillus subtilis cells during mild sorbic acid stress to reveal the growth-inhibitory activity of this preservative and to identify po...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01516-07
更新日期:2008-03-01 00:00:00
abstract::Bacillus thuringiensis var. thuringiensis Berliner grown at 14 C on a chemically defined liquid medium slowly produced spores and parasporal inclusions. Sporulation ultimately attained 50 to 100% of that of cultures grown at 30 C. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.1.445-448.1971
更新日期:1971-01-01 00:00:00
abstract::Bifidobacterium breve is an actinobacterium frequently isolated from colonic microbiota of breastfeeding babies. Here, we report the complete and annotated genome sequence of a B. breve strain isolated from human milk, B. breve CECT 7263. The genome sequence will provide new insights into the biology of this potential...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00691-12
更新日期:2012-07-01 00:00:00
abstract::A comprehensive equation, upsilon = VM/[1 + (A0.5/Fru-P2)n] [ 1 + (Glc-6-P/I0.5)], has been proposed to represent the quantitative interrelationships between the rate of glucose utilization and the levels of glucose-6-phosphate and fructose-1,6-diphosphate in the intact Escherichia coli cell. This comprehensive equati...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.128.1.165-169.1976
更新日期:1976-10-01 00:00:00
abstract::Cadmium-resistant Pseudomonas putida GAM-1, which was able to grow in concentrations of CdCl2 as high as 7 mM, was isolated from soil in a rice paddy. This bacterium harbored a DNA plasmid of about 52 kilobases. The plasmid (pGU100) transformed Escherichia coli C600 to cadmium resistance. A cadmium-resistant transform...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.165.1.334-335.1986
更新日期:1986-01-01 00:00:00
abstract::Escherichia coli produces polysaccharide capsules that, based on their mechanisms of synthesis and assembly, have been classified into four groups. The group 4 capsule (G4C) polysaccharide is frequently identical to that of the cognate lipopolysaccharide O side chain and has, therefore, also been termed the O-antigen ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.15.5259-5266.2005
更新日期:2005-08-01 00:00:00
abstract::Perry, Jerome J. (North Carolina State University, Raleigh), and James B. Evans. Oxidation and assimilation of carbohydrates by Micrococcus sodonensis. J. Bacteriol. 91:33-38. 1966.-Micrococcus sodonensis is a biotin-requiring strict aerobe that cannot utilize carbohydrates as sole sources of carbon and energy. Howeve...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.1.33-38.1966
更新日期:1966-01-01 00:00:00
abstract::The pathogenic bacterium Bacillus anthracis has become the subject of intense study as a result of its use in a bioterrorism attack in the United States in September and October 2001. Previous studies suggested that B. anthracis Ames Ancestor, the original Ames fully virulent plasmid-containing isolate, was the ideal ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01347-08
更新日期:2009-01-01 00:00:00
abstract::The psbAII gene of the cyanobacterium Synechococcus sp. strain PCC 7942 is a member of a three-gene family that encodes the D1 protein of the photosystem II reaction center. Transcription of psbAII is rapidly induced when the light intensity reaching the culture increases from 125 microE.m-2.s-1 (low light) to 750 mic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.3.508-516.1995
更新日期:1995-02-01 00:00:00
abstract::In aquatic environments, Caulobacter spp. can be found at the boundary between liquid and air known as the neuston. I report an approach to study temporal features of Caulobacter crescentus colonization and pellicle biofilm development at the air-liquid interface and have defined the role of cell surface structures in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00064-19
更新日期:2019-08-22 00:00:00
abstract::PcsB is a protein of unknown function that plays a critical role in cell division in Streptococcus pneumoniae and other ovococcus species of Streptococcus. We constructed isogenic sets of mutants expressing different amounts of PcsB in laboratory strain R6 and virulent serotype 2 strain D39 to evaluate its cellular ro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01505-08
更新日期:2009-05-01 00:00:00
abstract::The two closely linked fdhD and fdhE genes of Escherichia coli are required for the formation of active membrane-bound phenazine methosulfate-linked formate dehydrogenase (FDH-PMS). Both genes were isolated from a cosmid library. Restriction endonuclease analysis associated with Mu dII1734 insertion mutagenesis indica...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.10.6112-6121.1990
更新日期:1990-10-01 00:00:00
abstract::The DNA sequence of the central regulatory locus vir of Bordetella pertussis predicts that three gene products, BvgA, BvgB, and BvgC, are encoded. Features of the predicted primary structures of these proteins and their homology to other two-component systems suggest that BvgA is located in the cytoplasm, BvgB is loca...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.14.4288-4296.1991
更新日期:1991-07-01 00:00:00