A dominant sulfhydryl-containing protein in the outer membrane of Neisseria gonorrhoeae.

abstract：:The hexC locus of Pseudomonas aeruginosa PAO1 was localized to a 247-bp segment of chromosomal DNA on the multicopy broad-host-range vector pRO1614. The presence of this plasmid (pPZ196) in strain PAO1 produced the so-called "hexC effect," a two- to ninefold increase in the activities of four carbohydrate catabolism e...

journal_title：Journal of bacteriology

authors： Temple L,Sage A,Christie GE,Phibbs PV Jr

更新日期：1994-08-01 00:00:00

abstract：:Twenty-four-hour-old, aerobically grown, Luria-Bertani broth cultures of Salmonella typhimurium F98 suppressed the growth of a spectinomycin-resistant (Spcr) derivative of the same strain inoculated at 10(3) CFU ml(-1). This growth suppression is genus specific and RpoS independent, and it is not solely a result of nu...

journal_title：Journal of bacteriology

authors： Zhang-Barber L,Turner AK,Martin G,Frankel G,Dougan G,Barrow PA

更新日期：1997-11-01 00:00:00

abstract：:A conjugation system initially discovered in beta-lactamase-producing gonococci mobilized small non-selftransmissible R plasmids encoding beta-lactamase (penicillinase) production into other gonococci, Neisseria, and Escherichia coli. This conjugation system was mediated by a separate selftransmissible plasmid of 23.9...

journal_title：Journal of bacteriology

authors： Sox TE,Mohammed W,Blackman E,Biswas G,Sparling PF

更新日期：1978-04-01 00:00:00

abstract：:The mutant sigA allele of Bacillus subtilis DB1005 was confirmed to be temperature sensitive (ts) and transferable among strains of B. subtilis by chromosomal transformation and gene conversion. This ts sigA allele had a pleiotropic effect on gene expression of DB1005. The induction of certain heat shock proteins in D...

journal_title：Journal of bacteriology

authors： Chang BY,Chen KY,Wen YD,Liao CT

更新日期：1994-06-01 00:00:00

abstract：:Lag is a temporary period of nonreplication seen in bacteria that are introduced to new media. Despite latency being described by Müller in 1895, only recently have we gained insights into the cellular processes characterizing lag phase. This review covers literature to date on the transcriptomic, proteomic, metabolom...

journal_title：Journal of bacteriology

authors： Bertrand RL

更新日期：2019-03-13 00:00:00

abstract：:Site-directed mutagenesis has been employed to address the functional significance of the highly conserved aspartic and glutamic acid residues present in the Walker B (also called motif II) sequence in Escherichia coli DNA helicase II. Two mutant proteins, UvrDE221Q and UvrDD220NE221Q, were expressed and purified to a...

journal_title：Journal of bacteriology

authors： Brosh RM Jr,Matson SW

更新日期：1995-10-01 00:00:00

abstract：:Tracer studies of pyrimidine biosynthesis in Lactobacillus leichmannii (ATCC 7830) indicated that, while aspartate is utilized in the usual manner, the guanido carbon of arginine, rather than carbon dioxide, is utilized as a pyrimidine precursor. The guanido carbon of arginine also contributes, to some extent, to the ...

journal_title：Journal of bacteriology

authors： Hutson JY,Downing M

更新日期：1968-10-01 00:00:00

abstract：:The master regulator for entry into sporulation in Bacillus subtilis is the response regulator Spo0A, which directly governs the expression of about 121 genes. Using cells in which the synthesis of Spo0A was under the control of an inducible promoter or in which production of the regulatory protein was impaired by a p...

journal_title：Journal of bacteriology

authors： Fujita M,González-Pastor JE,Losick R

更新日期：2005-02-01 00:00:00

abstract：:Glycerol is a primary energy source for heterotrophic haloarchaea and a major component of "salty" biodiesel waste. Glycerol is catabolized solely by glycerol kinase (encoded by glpK) to glycerol-3-phosphate (G3P) in Haloferax volcanii. Here we characterized the next critical step of this metabolic pathway: the conver...

journal_title：Journal of bacteriology

authors： Rawls KS,Martin JH,Maupin-Furlow JA

更新日期：2011-09-01 00:00:00

abstract：:To study the role of the cis-acting element(s) in controlling the expression of the cytochrome P450(BM-1) gene and its upstream regulatory gene, bm1P1, in Bacillus megaterium, various deletion derivatives were constructed. A 53-bp inverted repeat located midway between the P450(BM-1) gene and bm1P1 gene was found in v...

journal_title：Journal of bacteriology

authors： Shaw GC,Sung CC,Liu CH,Kao HS

更新日期：1997-01-01 00:00:00

abstract：:Optochin-resistant mutant and wild-type diaphorases were purified approximately 300-fold by a combination of batch adsorption and column chromatography with diethylaminoethyl cellulose, and were characterized with regard to their pH optima, sensitivity to optochin inhibition and heat inactivation, Michaelis constants ...

journal_title：Journal of bacteriology

authors： Kreger AS,Olsen RH

更新日期：1968-10-01 00:00:00

abstract：:Environmental iron concentrations coordinately regulate transcription of genes involved in iron acquisition and virulence via the ferric uptake regulation (fur) system. We identified and sequenced the fur gene and flanking regions of three Bartonella species. The most notable difference between Bartonella Fur and othe...

journal_title：Journal of bacteriology

authors： Park SY,Kelminson KL,Lee AK,Zhang P,Warner RE,Rehkopf DH,Calderwood SB,Koehler JE

更新日期：2001-10-01 00:00:00

abstract：:Escherichia coli K-12 strains contain a cytoplasmic activity, peptidase N, capable of hydrolyzing alanine-p-nitroanilide. Mutations in the structural gene for the enzyme, pepN, were mapped, and the properties of mutant strains were examined. The pepN locus lay between ompF and asnS at approximately 20.8 min on the E. ...

journal_title：Journal of bacteriology

authors： McCaman MT,McPartland A,Villarejo MR

更新日期：1982-11-01 00:00:00

abstract：:We have constructed a series of broad-host-range plasmids which use "visual screens" to detect promoter activity. These plasmids contain the pMB1 and pRO1600 origins of replication and are capable of replicating in a wide range of gram-negative bacteria. The genes encoding beta-galactosidase and alkaline phosphatase f...

journal_title：Journal of bacteriology

authors： Farinha MA,Kropinski AM

更新日期：1990-06-01 00:00:00

abstract：:Glycosylation is a common modulation of protein function in eukaryotes and is biologically important. However, in bacteria protein glycosylation is rare, and relatively few bacterial glycoproteins are known. In Escherichia coli only two glycoproteins have been described to date. Here we introduce a novel member to thi...

journal_title：Journal of bacteriology

authors： Sherlock O,Dobrindt U,Jensen JB,Munk Vejborg R,Klemm P

更新日期：2006-03-01 00:00:00

abstract：:The highly conserved DnaK chaperones consist of an N-terminal ATPase domain, a central substrate-binding domain, and a C-terminal domain whose function is not known. Since Bacillus subtilis dnaK was not able to complement an Escherichia coli dnaK null mutant, we performed domain element swap experiments to identify th...

journal_title：Journal of bacteriology

authors： Mogk A,Bukau B,Lutz R,Schumann W

更新日期：1999-03-01 00:00:00

abstract：:The proteome of Haemophilus influenzae strain Rd KW20 was analyzed by liquid chromatography (LC) coupled with ion trap tandem mass spectrometry (MS/MS). This approach does not require a gel electrophoresis step and provides a rapidly developed snapshot of the proteome. In order to gain insight into the central metabol...

journal_title：Journal of bacteriology

authors： Kolker E,Purvine S,Galperin MY,Stolyar S,Goodlett DR,Nesvizhskii AI,Keller A,Xie T,Eng JK,Yi E,Hood L,Picone AF,Cherny T,Tjaden BC,Siegel AF,Reilly TJ,Makarova KS,Palsson BO,Smith AL

更新日期：2003-08-01 00:00:00

abstract：:Streptococcus pyogenes (group A streptococcus [GAS]), a catalase-negative gram-positive bacterium, is aerotolerant and survives H2O2 exposures that kill many catalase-positive bacteria. The molecular basis of the H2O2 resistance is poorly known. Here, we demonstrate that serotype M49 GAS lacking the Rgg regulator is m...

journal_title：Journal of bacteriology

authors： Pulliainen AT,Hytönen J,Haataja S,Finne J

更新日期：2008-05-01 00:00:00

abstract：:The molecular basis of the ability of bacteria to live on caffeine via the C-8 oxidation pathway is unknown. The first step of this pathway, caffeine to trimethyluric acid (TMU), has been attributed to poorly characterized caffeine oxidases and a novel quinone-dependent caffeine dehydrogenase. Here, we report the deta...

journal_title：Journal of bacteriology

authors： Mohanty SK,Yu CL,Das S,Louie TM,Gakhar L,Subramanian M

更新日期：2012-08-01 00:00:00

abstract：:Six mycoplasma strains, isolated under anaerobic conditions from the human oropharynx, were studied by biologic and serologic means. The strains produced nippled colonies with weak hemolytic activity for guinea pig erythrocytes on agar medium. In addition, the strains metabolized arginine with a concomitant alkaline s...

journal_title：Journal of bacteriology

authors： Fox H,Purcell RH,Chanock RM

更新日期：1969-04-01 00:00:00

abstract：:The plasmid-encoded citrate determinant of the Lactococcus lactis subsp. lactis var. diacetylactis NCDO176 was cloned and functionally expressed in a Cit- Escherichia coli K-12 strain. From deletion derivative analysis, a 3.4-kilobase region was identified which encodes the ability to transport citrate. Analysis of pr...

journal_title：Journal of bacteriology

authors： David S,van der Rest ME,Driessen AJ,Simons G,de Vos WM

更新日期：1990-10-01 00:00:00

abstract：:Membrane fractions from a lon strain of Escherichia coli but not a wild-type strain catalyze the incorporation of fucose from guanosine 5'-diphosphate-fucose into a lipid and into polymeric material. Both incorporation reactions specifically require only uridine 5'-diphosphate (UDP)-glucose. The sugar lipid was shown ...

journal_title：Journal of bacteriology

authors： Johnson JG,Wilson DB

更新日期：1977-01-01 00:00:00

abstract：:By extracellular labeling of peptides of intact Corynebacterium diphtheriae, followed by fractionation of the cells and chain completion by isolated polysomes, it is shown that diphtheria toxin is formed and secreted cotranslationally by membrane-bound polysomes; free polysomes from none. Moreover, when the chains on ...

journal_title：Journal of bacteriology

authors： Smith WP,Tai PC,Murphy JR,Davis BD

更新日期：1980-01-01 00:00:00

abstract：:Synthesis of formate dehydrogenase coupled to formate hydrogenlyase activity in Escherichia coli was found to require the product of the fhlA gene. Transcription of fdhF, the gene coding for the 80-kilodalton (kDa) selenopeptide of formate dehydrogenase, was not detected in an fhlA genetic background. Mutations in the...

journal_title：Journal of bacteriology

authors： Sankar P,Lee JH,Shanmugam KT

更新日期：1988-12-01 00:00:00

abstract：:A mutational analysis of lesion-forming ability was undertaken in Pseudomonas syringae pv. syringae B728a, causal agent of bacterial brown spot disease of bean. Following a screen of 6,401 Tn5-containing derivatives of B728a on bean pods, 26 strains that did not form disease lesions were identified. Nine of the mutant...

journal_title：Journal of bacteriology

authors： Rich JJ,Willis DK

更新日期：1997-04-01 00:00:00

abstract：:The sequences of the capsular biosynthetic (cps) loci of 90 serotypes of Streptococcus pneumoniae have recently been determined. Bioinformatic procedures were used to predict the general functions of 1,973 of the 1,999 gene products and to identify proteins within the same homology group, Pfam family, and CAZy glycosy...

journal_title：Journal of bacteriology

authors： Aanensen DM,Mavroidi A,Bentley SD,Reeves PR,Spratt BG

更新日期：2007-11-01 00:00:00

abstract：:In Escherichia coli, T:G mismatches in specific contexts are corrected by a very short patch (VSP) repair system. Previous studies have shown that the product of gene vsr mediates correction of T:G to C:G in the 5'CTAGG/3'GGTCC context and in some related contexts. Amber mutations that arose in CAG sequences in gene c...

journal_title：Journal of bacteriology

authors： Lieb M,Rehmat S

更新日期：1995-02-01 00:00:00

abstract：:Growth of a derivative of Escherichia coli K-10 was strongly inhibited by 2 times 10(-4) M L-5(1-amino-2-methylbutyl)-tetrazole (isoleucine tetrazole). Growth inhibition was reversed by isoleucine, threonine, glycyl-L-isoleucine, or glycyl-L-threonine, and, in a valine-resistant mutant, by L-valine. Partial reversal o...

journal_title：Journal of bacteriology

authors： Willshaw GA,Tristram H

更新日期：1975-09-01 00:00:00

abstract：:Studies on the active product of pepsin digestion of rabbit immunoglobulin G (IgG), F(ab')(2) produced from antiserum against Neurospora glutamate dehydrogenase, showed it to behave in the same way, in both antienzyme and precipitation experiments, as homologous IgG. It is proposed that the inhibition of glutamate deh...

journal_title：Journal of bacteriology

authors： Roberts DB

更新日期：1967-10-01 00:00:00

abstract：:The enzymatic properties and the physiological function of the type IV apurinic/apyrimidinic (AP)-endonuclease homolog of Bacillus subtilis, encoded by yqfS, a gene specifically expressed in spores, were studied here. To this end, a recombinant YqfS protein containing an N-terminal His6 tag was synthesized in Escheric...

journal_title：Journal of bacteriology

authors： Salas-Pacheco JM,Urtiz-Estrada N,Martínez-Cadena G,Yasbin RE,Pedraza-Reyes M

更新日期：2003-09-01 00:00:00