Genetics and regulation of peptidase N in Escherichia coli K-12.

Abstract:

:Escherichia coli K-12 strains contain a cytoplasmic activity, peptidase N, capable of hydrolyzing alanine-p-nitroanilide. Mutations in the structural gene for the enzyme, pepN, were mapped, and the properties of mutant strains were examined. The pepN locus lay between ompF and asnS at approximately 20.8 min on the E. coli chromosome. Loss of peptidase N activity through mutation had no apparent effect on the growth rate or nutritional needs of the cell. Enzyme levels in wild-type strains were constant throughout the growth cycle and were constitutive in all of the growth media tested. Starvation for carbon, nitrogen, or phosphate also did not alter enzyme levels. Constitutive expression of peptidase N is consistent with the idea that the enzyme plays a significant role in the degradation of intracellularly generated peptides.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

McCaman MT,McPartland A,Villarejo MR

subject

Has Abstract

pub_date

1982-11-01 00:00:00

pages

848-54

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

152

pub_type

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