Abstract:
:The DNA sequence of the central regulatory locus vir of Bordetella pertussis predicts that three gene products, BvgA, BvgB, and BvgC, are encoded. Features of the predicted primary structures of these proteins and their homology to other two-component systems suggest that BvgA is located in the cytoplasm, BvgB is located in the periplasm, and BvgC spans the inner membrane. We have used gene fusions to the phoA and lacZ genes of Escherichia coli to investigate the subcellular localization and membrane topology of these proteins. PhoA fusion proteins were also purified and used to raise antibodies that allowed visualization of the vir-encoded polypeptides by Western immunoblotting. Our results have largely confirmed the predictions of the DNA sequence, with the exception that BvgB and BvgC were found to constitute one larger protein that was homologous to the sensor class of two-component systems. We propose that this protein be named BvgS (for sensor) and that its gene be named bvgS.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Stibitz S,Yang MSdoi
10.1128/jb.173.14.4288-4296.1991subject
Has Abstractpub_date
1991-07-01 00:00:00pages
4288-96issue
14eissn
0021-9193issn
1098-5530journal_volume
173pub_type
杂志文章abstract:UNLABELLED:FimV is a Pseudomonas aeruginosa inner membrane protein that regulates intracellular cyclic AMP (cAMP) levels-and thus type IV pilus (T4P)-mediated twitching motility and type II secretion (T2S)-by activating the adenylate cyclase CyaB. Its cytoplasmic domain contains three predicted tetratricopeptide repeat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00322-16
更新日期:2016-07-28 00:00:00
abstract::Acinetobacter baumannii has emerged as a significant nosocomial pathogen worldwide. The increasing trend of carbapenem and fluoroquinolone resistance in A. baumannii severely limits the usage of therapeutic antimicrobial agents. Here we report the genome sequence of a multidrug-resistant A. baumannii strain, TCDC-AB07...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00244-11
更新日期:2011-05-01 00:00:00
abstract:UNLABELLED:Multiple essential small GTPases are involved in the assembly of the ribosome or in the control of its activity. Among them, ObgE (CgtA) has been shown recently to act as a ribosome antiassociation factor that binds to ppGpp, a regulator whose best-known target is RNA polymerase. The present study was aimed ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00159-16
更新日期:2016-06-13 00:00:00
abstract::The foliar pathogen Pseudomonas syringae is a useful model for understanding the role of stress adaptation in leaf colonization. We investigated the mechanistic basis of differences in the osmotolerance of two P. syringae strains, B728a and DC3000. Consistent with its higher survival rates following inoculation onto l...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00787-13
更新日期:2013-10-01 00:00:00
abstract::Sequences essential to the chloramphenicol-inducible expression of cat-86, a chloramphenicol acetyltransferase gene, reside in a 144-base pair (bp) regulatory region that intervenes between the cat-86 coding sequence and its promoter. A key regulatory element within the 144-bp segment consists of a pair of inverted-re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.2.696-703.1985
更新日期:1985-11-01 00:00:00
abstract::Phosphate import is required for the growth of mycobacteria and is regulated by environmental inorganic phosphate (P(i)) concentrations, although the mechanism of this regulation has not been characterized. The expression of genes involved in P(i) acquisition is frequently regulated by two-component regulatory systems...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00190-07
更新日期:2007-08-01 00:00:00
abstract::The genes that encode the two subunits of Bacillus subtilis phenylalanyl-tRNA synthetase were cloned from alpha lambda library of chromosomal B. subtilis DNA by specific complementation of a thermosensitive Escherichia coli pheS mutation. Both genes (we named them pheS and pheT, analogous to the corresponding genes of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.2.1228-1232.1989
更新日期:1989-02-01 00:00:00
abstract::tRNAs play a critical role in mRNA decoding, and posttranscriptional modifications within tRNAs drive decoding efficiency and accuracy. The types and positions of tRNA modifications in model bacteria have been extensively studied, and tRNA modifications in a few eukaryotic organisms have also been characterized and lo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00690-18
更新日期:2019-04-09 00:00:00
abstract::The recF gene of Escherichia coli is known to encode an Mr-40,000 protein that is involved in DNA recombinationa nd postreplication DNA repair. To characterize the role of the recF gene product in these processes, the recF gene was cloned downstream of a tac promoter to facilitate overproduction of the recF gene produ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.11.6291-6299.1990
更新日期:1990-11-01 00:00:00
abstract::The DNA sequence of part of the gnd (6-phosphogluconate dehydrogenase) gene was determined for eight wild strains of Escherichia coli and for Salmonella typhimurium. Since a region of the trp (tryptophan) operon and the phoA (alkaline phosphatase) gene have been sequenced from the same strains, the gene trees for thes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.22.7257-7268.1991
更新日期:1991-11-01 00:00:00
abstract::The outer membrane of the thermophilic bacterium Thermus thermophilus was isolated using sucrose step gradient centrifugation. Its detergent extracts contained an ion-permeable channel with an extremely high single-channel conductance of 20 nS in 1 M KCl. The channel protein was purified by preparative sodium dodecyl ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.2.800-803.2001
更新日期:2001-01-01 00:00:00
abstract::Gerhardt, Philipp (University of Michigan, Ann Arbor) and S. H. Black. Permeability of bacterial spores. II. Molecular variables affecting solute permeation. J. Bacteriol. 82:750-760. 1961.-More than 100 compounds were tested for their uptake by dormant spores of a bacillus. The extent of penetration was found to be d...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.5.750-760.1961
更新日期:1961-11-01 00:00:00
abstract::YoeB is a bacterial toxin encoded by the yefM-yoeB toxin-antitoxin system found in various bacterial genomes. Here, we show that Staphylococcus aureus contains two YoeB homologues, both of which function as ribosome-dependent mRNA interferases to inhibit translation initiation in a manner identical to that of YoeB-ec ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00623-09
更新日期:2009-09-01 00:00:00
abstract::Coscarelli, Waldimero (Rutgers, The State University, New Brunswick, N.J.) and David Pramer. Nutrition and growth of Arthrobotrys conoides. J. Bacteriol. 84:60-64. 1962-Despite its ability to capture, kill, and subsist on nematodes, the predacious fungus Arthrobotrys conoides does not demonstrate a unique nutritional ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.1.60-64.1962
更新日期:1962-07-01 00:00:00
abstract::Agrobacterium radiobacter NCIB 11883 was grown in lactose-limited continuous culture at a dilution rate of 0.045/h. Washed cells transported [14C]lactose and [methyl-14C]beta-D-thiogalactoside, a nonmetabolisable analog of lactose, at similar rates and with similar affinities (Km for transport, less than 1 microM). Tr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.4.1703-1710.1990
更新日期:1990-04-01 00:00:00
abstract::The O polysaccharide of the lipopolysaccharide (O antigen) of Gram-negative bacteria often serves as a receptor for bacteriophages that can make the phage dependent on a given O-antigen type, thus supporting the concept of the adaptive significance of the O-antigen variability in bacteria. The O-antigen layer also mod...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02398-14
更新日期:2015-03-01 00:00:00
abstract::Pseudomonas aeruginosa PAO1 catabolized the aromatic amines tyramine and octopamine through 4-hydroxyphenylacetic acid and 3,4-dihydroxyphenylacetic acid (HPA). meta ring cleavage was mediated by 3,4-dihydroxyphenylacetate 2,3-dioxygenase (HPADO), producing 2-hydroxy-5-carboxymethylmuconic semialdehyde (MSA). An NAD-d...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.1.393-399.1988
更新日期:1988-01-01 00:00:00
abstract::MntH, a bacterial homolog of mammalian natural resistance associated macrophage protein 1 (Nramp1), is a primary transporter for Mn(2+) influx in Salmonella enterica serovar Typhimurium and Escherichia coli. S. enterica serovar Typhimurium MntH contributes to H(2)O(2) resistance and is important for full virulence. Co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.12.3151-3158.2002
更新日期:2002-06-01 00:00:00
abstract::Hydrogenotrophic methanogenic Archaea are defined by an H2 requirement for growth. Despite this requirement, many hydrogenotrophs are also capable of growth with formate as an electron donor for methanogenesis. While certain responses of these organisms to hydrogen availability have been characterized, responses to fo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02141-12
更新日期:2013-04-01 00:00:00
abstract::Carbon catabolite control protein A (CcpA) is the main regulator involved in carbon catabolite repression in gram-positive bacteria. Time series gene expression analyses of Lactococcus lactis MG1363 and L. lactis MG1363DeltaccpA using DNA microarrays were used to define the CcpA regulon of L. lactis. Based on a compar...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01013-06
更新日期:2007-02-01 00:00:00
abstract::In the nitrogenase system of Klebsiella pneumoniae, nifT is located between nifDK, the structural genes for dinitrogenase, and nifY, whose product is involved in nitrogenase maturation. It is, therefore, a reasonable hypothesis that the NifT protein might also have a role in the maturation of nitrogenase. However, the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.10.2975-2977.1996
更新日期:1996-05-01 00:00:00
abstract::Current mouse models for evaluating the efficacy of live oral cholera vaccines (OCVs) have important limitations. Conventionally raised adult mice are resistant to intestinal colonization by Vibrio cholerae, but germfree mice can be colonized and have been used to study OCV immunogenicity. However, germfree animals ha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00232-20
更新日期:2020-11-19 00:00:00
abstract::The model archaeon Pyrococcus furiosus grows optimally near 100°C on carbohydrates and peptides. Its genome sequence (NCBI) was determined 12 years ago. A genetically tractable strain, COM1, was very recently reported, and here we describe its genome sequence. Of 1,909,827 bp in size, it is 1,571 bp longer (0.1%) than...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00439-12
更新日期:2012-08-01 00:00:00
abstract::Erwinia chrysanthemi 3937 secretes several pectinolytic enzymes, among which eight isoenzymes of pectate lyases with an endo-cleaving mode (PelA, PelB, PelC, PelD, PelE, PelI, PelL, and PelZ) have been identified. Two exo-cleaving enzymes, the exopolygalacturonate lyase, PelX, and an exo-poly-alpha-D-galacturonosidase...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.5.1652-1663.1999
更新日期:1999-03-01 00:00:00
abstract::Genome sequencing revealed that all six chlamydiae genomes contain three groEL-like genes (groEL1, groEL2, and groEL3). Phylogenetic analysis of groEL1, groEL2, and groEL3 indicates that these genes are likely to have been present in chlamydiae since the beginning of the lineage. Comparison of deduced amino acid seque...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.6.1958-1966.2003
更新日期:2003-03-01 00:00:00
abstract::Kobayashi, George S. (Tulane University, New Orleans, La.), Lorraine Friedman, and Judith F. Kofroth. Some cytological and pathogenic properties of spheroplasts of Candida albicans. J. Bacteriol. 88:795-801. 1964.-Spheroplasts of Candida albicans were prepared by use of an enzymatic mixture from the digestive tract of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.3.795-801.1964
更新日期:1964-09-01 00:00:00
abstract::Thermus thermophilus HB8 can grow anaerobically by using a membrane-bound nitrate reductase to catalyze the reduction of nitrate as a final electron acceptor in respiration. In contrast to other denitrifiers, the nitrite produced does not continue the reduction pathway but accumulates in the growth medium after its ac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.8.2179-2183.2000
更新日期:2000-04-01 00:00:00
abstract::A mutant of Escherichia coli resistant to serine hydroxamate which has a large increase in Km for serine of seryl-tRNA synthetase is described. The mutant serS gene was cloned and sequenced and was found to contain a single-base-pair mutation, resulting in the substitution of the residue alanine 262 by valine in motif...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.11.3347-3350.1995
更新日期:1995-06-01 00:00:00
abstract::The enzymes LinB(UT) and LinB(MI) (LinB from Sphingobium japonicum UT26 and Sphingobium sp. MI1205, respectively) catalyze the hydrolytic dechlorination of β-hexachlorocyclohexane (β-HCH) and yield different products, 2,3,4,5,6-pentachlorocyclohexanol (PCHL) and 2,3,5,6-tetrachlorocyclohexane-1,4-diol (TCDL), respecti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02020-12
更新日期:2013-06-01 00:00:00
abstract::By using a method that labels sulfhydryl-containing proteins in situ, we have detected a major outer membrane protein of Neisseria gonorrhoeae at 41 kDa. A protein of this molecular mass has not previously been shown to be a major outer membrane protein in gonococci. In addition, a minor protein rich in cysteinyl resi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.4.1173-1175.1993
更新日期:1993-02-01 00:00:00