Transient Intestinal Colonization by a Live-Attenuated Oral Cholera Vaccine Induces Protective Immune Responses in Streptomycin-Treated Mice.

Abstract:

:Current mouse models for evaluating the efficacy of live oral cholera vaccines (OCVs) have important limitations. Conventionally raised adult mice are resistant to intestinal colonization by Vibrio cholerae, but germfree mice can be colonized and have been used to study OCV immunogenicity. However, germfree animals have impaired immune systems and intestinal physiology; also, live OCVs colonize germfree mice for many months, which does not mimic the clearance kinetics of live OCVs in humans. In this study, we leveraged antibiotic-treated, conventionally raised adult mice to study the effects of transient intestinal colonization by a live OCV V. cholerae strain. In a single-dose vaccination regimen, we found that HaitiV, a live-attenuated OCV candidate, was cleared by streptomycin-treated adult mice within 2 weeks after oral inoculation. This transient colonization elicited far stronger adaptive immune correlates of protection against cholera than did inactivated whole-cell HaitiV. Infant mice from HaitiV-vaccinated dams were also significantly more protected from choleric disease than pups from inactivated-HaitiV-vaccinated dams. Our findings establish the benefits of antibiotic-treated mice for live-OCV studies as well as their limitations and underscore the immunogenicity of HaitiV.IMPORTANCE Oral cholera vaccines (OCVs) are being deployed to combat cholera, but current killed OCVs require multiple doses and show little efficacy in young children. Live OCVs have the potential to overcome these limitations, but small-animal models for testing OCVs have shortcomings. We used an antibiotic treatment protocol for conventional adult mice to study the effects of short-term colonization by a single dose of HaitiV, a live-OCV candidate. Vaccinated mice developed vibriocidal antibodies against V. cholerae and delivered pups that were resistant to cholera, whereas mice vaccinated with inactivated HaitiV did not. These findings demonstrate HaitiV's immunogenicity and suggest that this antibiotic treatment protocol will be useful for evaluating the efficacy of live OCVs.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Fakoya B,Sit B,Waldor MK

doi

10.1128/JB.00232-20

subject

Has Abstract

pub_date

2020-11-19 00:00:00

issue

24

eissn

0021-9193

issn

1098-5530

pii

JB.00232-20

journal_volume

202

pub_type

杂志文章
  • Dynamics of the SetCD-regulated integration and excision of genomic islands mobilized by integrating conjugative elements of the SXT/R391 family.

    abstract::Mobilizable genomic islands (MGIs) are small genomic islands that are mobilizable by SXT/R391 integrating conjugative elements (ICEs) due to similar origins of transfer. Their site-specific integration and excision are catalyzed by the integrase that they encode, but their conjugative transfer entirely depends upon th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01093-12

    authors: Daccord A,Mursell M,Poulin-Laprade D,Burrus V

    更新日期:2012-11-01 00:00:00

  • Thickness and elasticity of gram-negative murein sacculi measured by atomic force microscopy.

    abstract::Atomic force microscopy was used to measure the thickness of air-dried, collapsed murein sacculi from Escherichia coli K-12 and Pseudomonas aeruginosa PAO1. Air-dried sacculi from E. coli had a thickness of 3.0 nm, whereas those from P. aeruginosa were 1.5 nm thick. When rehydrated, the sacculi of both bacteria swelle...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.22.6865-6875.1999

    authors: Yao X,Jericho M,Pink D,Beveridge T

    更新日期:1999-11-01 00:00:00

  • Characterization of the detachable Rho-dependent transcription terminator of the fimE gene in Escherichia coli K-12.

    abstract::The fim genetic switch in the chromosome of Escherichia coli K-12 is an invertible DNA element that harbors the promoter for transcription of the downstream fim structural genes and a transcription terminator that acts on the upstream fimE regulatory gene. Switches oriented appropriately for structural gene transcript...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.24.8256-8266.2005

    authors: Hinde P,Deighan P,Dorman CJ

    更新日期:2005-12-01 00:00:00

  • Cloning of the ADPglucose pyrophosphorylase (glgC) and glycogen synthase (glgA) structural genes from Salmonella typhimurium LT2.

    abstract::The structural genes of ADPglucose pyrophosphorylase (glgC) and glycogen synthase (glgA) from Salmonella typhimurium LT2 were cloned on a 5.8-kilobase-pair insert in the SalI site of pBR322. A single strand specific radioactive probe containing the N terminus of the Escherichia coli K-12 glgC gene in M13mp8 was used t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.9.4349-4354.1987

    authors: Leung PS,Preiss J

    更新日期:1987-09-01 00:00:00

  • D-erythro-neopterin biosynthesis in the methanogenic archaea Methanococcus thermophila and Methanobacterium thermoautotrophicum deltaH.

    abstract::The steps in the biosynthetic transformation of GTP to 7,8-dihydro-D-erythro-neopterin (H2neopterin), the precursor to the modified folates found in the methanogenic archaea, has been elucidated for the first time in two members of the domain Archaea. In Methanococcus thermophila and Methanobacterium thermoautotrophic...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.16.5165-5170.1997

    authors: Howell DM,White RH

    更新日期:1997-08-01 00:00:00

  • Role of surface protein SasG in biofilm formation by Staphylococcus aureus.

    abstract::The SasG surface protein of Staphylococcus aureus has been shown to promote the formation of biofilm. SasG comprises an N-terminal A domain and repeated B domains. Here we demonstrate that SasG is involved in the accumulation phase of biofilm, a process that requires a physiological concentration of Zn(2+). The B doma...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00628-10

    authors: Geoghegan JA,Corrigan RM,Gruszka DT,Speziale P,O'Gara JP,Potts JR,Foster TJ

    更新日期:2010-11-01 00:00:00

  • Characterization and regulation of the genes for a novel anthranilate 1,2-dioxygenase from Burkholderia cepacia DBO1.

    abstract::Anthranilate (2-aminobenzoate) is an important intermediate in tryptophan metabolism. In order to investigate the degradation of tryptophan through anthranilate by Burkholderia cepacia, several plasposon mutations were constructed of strain DBO1 and one mutant with the plasposon insertion in the anthranilate dioxygena...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.19.5871-5881.2003

    authors: Chang HK,Mohseni P,Zylstra GJ

    更新日期:2003-10-01 00:00:00

  • Diversity of genome structure in Salmonella enterica serovar Typhi populations.

    abstract::The genomes of most strains of Salmonella and Escherichia coli are highly conserved. In contrast, all 136 wild-type strains of Salmonella enterica serovar Typhi analyzed by partial digestion with I-CeuI (an endonuclease which cuts within the rrn operons) and pulsed-field gel electrophoresis and by PCR have rearrangeme...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.8.2638-2650.2005

    authors: Kothapalli S,Nair S,Alokam S,Pang T,Khakhria R,Woodward D,Johnson W,Stocker BA,Sanderson KE,Liu SL

    更新日期:2005-04-01 00:00:00

  • S-adenosylmethionine-binding properties of a bacterial phospholipid N-methyltransferase.

    abstract::The presence of the membrane lipid phosphatidylcholine (PC) in the bacterial membrane is critically important for many host-microbe interactions. The phospholipid N-methyltransferase PmtA from the plant pathogen Agrobacterium tumefaciens catalyzes the formation of PC by a three-step methylation of phosphatidylethanola...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01539-10

    authors: Aktas M,Gleichenhagen J,Stoll R,Narberhaus F

    更新日期:2011-07-01 00:00:00

  • Isolation in high frequency of rough variants of Mycobacterium intracellulare lacking C-mycoside glycopeptidolipid antigens.

    abstract::Rough variants of serovars from the Mycobacterium avium-Mycobacterium intracellulare-Mycobacterium scrofulaceum complex were isolated in high frequency from pellicle growth of the wild-type strains. Rough morphology could be correlated with the lack of an outer cell wall sheath and its constituent C-mycoside glycopept...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.150.1.381-384.1982

    authors: Barrow WW,Brennan PJ

    更新日期:1982-04-01 00:00:00

  • RmpA regulation of capsular polysaccharide biosynthesis in Klebsiella pneumoniae CG43.

    abstract::Sequence analysis of the large virulence plasmid pLVPK in Klebsiella pneumoniae CG43 revealed the presence of another mucoid factor encoding gene rmpA besides rmpA2. Promoter activity measurement indicated that the deletion of rmpA reduced K2 capsular polysaccharide (CPS) biosynthesis, resulting in decreased colony mu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00031-10

    authors: Cheng HY,Chen YS,Wu CY,Chang HY,Lai YC,Peng HL

    更新日期:2010-06-01 00:00:00

  • Genetic and molecular characteristics of Vir plasmids of bovine septicemic Escherichia coli.

    abstract::Three wild strains of bovine septicemic Escherichia coli were selected on the basis of their production of a toxin lethal for mice and chickens and their characteristic surface antigen. The transfer of these virulence (Vir) properties from two of the three to recipient E. coli was detected after mating. One Vir plasmi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.2.758-769.1980

    authors: Lopez-Alvarez J,Gyles CL,Shipley PL,Falkow S

    更新日期:1980-02-01 00:00:00

  • Use of a novel mobilizable vector to inactivate the scrA gene of Streptococcus sobrinus by allelic replacement.

    abstract::The virulence factors of the cariogenic bacterium Streptococcus sobrinus have been difficult to assess because of a lack of tools for the genetic manipulation of this organism. The construction of an Escherichia coli-Streptococcus shuttle vector, pDL289, that can be mobilized into S. sobrinus by the conjugative plasmi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.17.5028-5034.1995

    authors: Buckley ND,Lee LN,LeBlanc DJ

    更新日期:1995-09-01 00:00:00

  • Outer membrane proteins and cell surface structure of Selenomonas ruminantium.

    abstract::The protein compositions of the membrane preparations from Selenomonas ruminantium grown in glucose or lactate medium were determined by sodium dodecyl sulfate- and two-dimensional (first, isoelectric focusing; second, sodium dodecyl sulfate) polyacrylamide slab gel electrophoresis. The outer membrane from both glucos...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.2.899-907.1980

    authors: Kamio Y,Takahashi H

    更新日期:1980-02-01 00:00:00

  • Impaired expression of certain prereplicative bacteriophage T4 genes explains impaired T4 DNA synthesis in Escherichia coli rho (nusD) mutants.

    abstract::The Escherichia coli rho 026 mutation that alters the transcription termination protein Rho prevents growth of wild-type bacteriophage T4. Among the consequences of this mutation are delayed and reduced T4 DNA replication. We show that these defects can be explained by defective synthesis of certain T4 replication-rec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.7.3872-3880.1989

    authors: Stitt BL,Mosig G

    更新日期:1989-07-01 00:00:00

  • Genetic, molecular, and functional analysis of Streptococcus faecalis R plasmid pJH1.

    abstract::Streptococcus faecalis JH1 contains two conjugative plasmids, pJH1, an R plasmid that codes for resistance to kanamycin, streptomycin, erythromycin, and tetracycline, and pJH2, a hemolysin-bacteriocin plasmid. Strain JH1 was used as an antibiotic resistance donor in conjugation experiments with two plasmid-free S. fae...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.155.3.1094-1104.1983

    authors: Banai M,LeBlanc DJ

    更新日期:1983-09-01 00:00:00

  • Horizontal transference of S-layer genes within Thermus thermophilus.

    abstract::The S-layers of Thermus thermophilus HB27 and T. thermophilus HB8 are composed of protein units of 95 kDa (P95) and 100 kDa (P100), respectively. We have selected S-layer deletion mutants from both strains by complete replacement of the slpA gene. Mutants of the two strains showed similar defects in growth and morphol...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.19.5460-5466.1995

    authors: Fernández-Herrero LA,Olabarría G,Castón JR,Lasa I,Berenguer J

    更新日期:1995-10-01 00:00:00

  • Escherichia coli K-12 can utilize an exogenous gamma-glutamyl peptide as an amino acid source, for which gamma-glutamyltranspeptidase is essential.

    abstract::Escherichia coli K-12 can utilize a gamma-glutamyl peptide as an amino acid source, for which gamma-glutamyltranspeptidase (EC 2.3.2.2) is essential. We suggest that the gamma-glutamyl linkage of a gamma-glutamyl peptide is hydrolyzed by gamma-glutamyltranspeptidase located in the periplasmic space, and the released a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.18.6038-6040.1993

    authors: Suzuki H,Hashimoto W,Kumagai H

    更新日期:1993-09-01 00:00:00

  • The Sakaguchi reaction product quenches phycobilisome fluorescence, allowing determination of the arginine concentration in cells of Anabaena strain PCC 7120.

    abstract::The filamentous cyanobacterium Anabaena fixes nitrogen in specialized cells called heterocysts. The immediate product of fixation, ammonia, is known to be assimilated by addition to glutamate to make glutamine. How fixed nitrogen is transported along the filament to the 10 to 20 vegetative cells that separate heterocy...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01512-12

    authors: Ke S,Haselkorn R

    更新日期:2013-01-01 00:00:00

  • Expression system for high levels of GAG lyase gene expression and study of the hepA upstream region in Flavobacterium heparinum.

    abstract::A system for high-level expression of heparinase I, heparinase II, heparinase III, chondroitinase AC, and chondroitinase B in Flavobacterium heparinum is described. hepA, along with its regulatory region, as well as hepB, hepC, cslA, and cslB, cloned downstream of the hepA regulatory region, was integrated in the chro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.12.3242-3252.2002

    authors: Blain F,Tkalec AL,Shao Z,Poulin C,Pedneault M,Gu K,Eggimann B,Zimmermann J,Su H

    更新日期:2002-06-01 00:00:00

  • CcpA-independent regulation of expression of the Mg2+ -citrate transporter gene citM by arginine metabolism in Bacillus subtilis.

    abstract::Transcriptional regulation of the Mg(2+)-citrate transporter, CitM, the main citrate uptake system of Bacillus subtilis, was studied during growth in rich medium. Citrate in the growth medium was required for induction under all growth conditions. In Luria-Bertani medium containing citrate, citM expression was complet...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.3.854-859.2003

    authors: Warner JB,Magni C,Lolkema JS

    更新日期:2003-02-01 00:00:00

  • Alternative transcription factor sigma(B) is involved in regulation of biofilm expression in a Staphylococcus aureus mucosal isolate.

    abstract::Osmotic stress was found to induce biofilm formation in a Staphylococcus aureus mucosal isolate. Inactivation of a global regulator of the bacterial stress response, the alternative transcription factor sigma(B), resulted in a biofilm-negative phenotype and loss of salt-induced biofilm production. Complementation of t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.23.6824-6826.2000

    authors: Rachid S,Ohlsen K,Wallner U,Hacker J,Hecker M,Ziebuhr W

    更新日期:2000-12-01 00:00:00

  • Inactivation of Lgt allows systematic characterization of lipoproteins from Listeria monocytogenes.

    abstract::Lipoprotein anchoring in bacteria is mediated by the prolipoprotein diacylglyceryl transferase (Lgt), which catalyzes the transfer of a diacylglyceryl moiety to the prospective N-terminal cysteine of the mature lipoprotein. Deletion of the lgt gene in the gram-positive pathogen Listeria monocytogenes (i) impairs intra...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00976-06

    authors: Baumgärtner M,Kärst U,Gerstel B,Loessner M,Wehland J,Jänsch L

    更新日期:2007-01-01 00:00:00

  • EspF of enteropathogenic Escherichia coli binds sorting nexin 9.

    abstract::EspF of enteropathogenic Escherichia coli targets mitochondria and subverts a number of cellular functions. EspF consists of six putative Src homology 3 (SH3) domain binding motifs. In this study we identified sorting nexin 9 (SNX9) as a host cell EspF binding partner protein, which binds EspF via its amino-terminal S...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.8.3110-3115.2006

    authors: Marchès O,Batchelor M,Shaw RK,Patel A,Cummings N,Nagai T,Sasakawa C,Carlsson SR,Lundmark R,Cougoule C,Caron E,Knutton S,Connerton I,Frankel G

    更新日期:2006-04-01 00:00:00

  • Immunogenicity of irradiated Salmonella typhimurium cells and endotoxin.

    abstract::The effects of high doses of radiation (1, 5, or 20 Mrad) on the toxicity, pyrogenicity, and immunogenicity of Salmonella typhimurium cells and endotoxin were studied. Toxicity decreased progressively after exposure to 1, 5, or 20 Mrad. The lethal effect of 1-Mrad exposed cells was greater than that of heat-, acetone-...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.6.2165-2170.1968

    authors: Previte JJ

    更新日期:1968-06-01 00:00:00

  • Physiological effects of Crl in Salmonella are modulated by sigmaS level and promoter specificity.

    abstract::The small regulatory protein Crl activates sigma(S) (RpoS), the stationary-phase and general stress response sigma factor. Crl has been reported to bind sigma(S) in vitro and to facilitate the formation of RNA polymerase holoenzyme. In Salmonella enterica serovar Typhimurium, Crl is required for the development of the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01919-06

    authors: Robbe-Saule V,Lopes MD,Kolb A,Norel F

    更新日期:2007-04-01 00:00:00

  • Replication of the nonconjugative plasmid RSF1010 in Escherichia coli K-12.

    abstract::Replicating DNA molecules of the nonconjugative R plasmid RSF1010 (Smr Sur) were cleaved with the EcoRI restriction endonuclease and examined with the electron microscope. Results of this analysis indicated that replication is initiated from an origin located at about 19% of total genome size from one of the EcoRI end...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.134.3.1117-1122.1978

    authors: de Graaff J,Crosa JH,Heffron F,Falkow S

    更新日期:1978-06-01 00:00:00

  • Galactose metabolism. I. Pathway of carbon in fermentation by Streptococcus faecalis.

    abstract::Fukuyama T. T. (University of Pennsylvania, Philadelphia) and D. J. O'Kane. Galactose metabolism. I. Pathway of carbon in fermentation by Streptococcus faecalis. J. Bacteriol. 84:793-796. 1962.-The pathway by which galactose-1-C(14) is fermented in Streptococcus faecalis was investigated using dried-cell preparations....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.84.4.793-796.1962

    authors: FUKUYAMA TT,O'KANE DJ

    更新日期:1962-10-01 00:00:00

  • Evolutionary analysis of Burkholderia pseudomallei identifies putative novel virulence genes, including a microbial regulator of host cell autophagy.

    abstract::Burkholderia pseudomallei, the causative agent of melioidosis, contains a large pathogen genome (7.2 Mb) with ∼2,000 genes of putative or unknown function. Interactions with potential hosts and environmental factors may induce rapid adaptations in these B. pseudomallei genes, which can be discerned through evolutionar...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00718-13

    authors: Singh AP,Lai SC,Nandi T,Chua HH,Ooi WF,Ong C,Boyce JD,Adler B,Devenish RJ,Tan P

    更新日期:2013-12-01 00:00:00

  • Insight into the Dual Functions of Bacterial Enhancer-Binding Protein Rrp2 of Borrelia burgdorferi.

    abstract:UNLABELLED:It is well established that the RpoN-RpoS sigma factor (σ(54)-σ(S)) cascade plays an essential role in differential gene expression during the enzootic cycle of Borrelia burgdorferi, the causative agent of Lyme disease. The RpoN-RpoS pathway is activated by the response regulator/σ(54)-dependent activator (a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01010-15

    authors: Yin Y,Yang Y,Xiang X,Wang Q,Yang ZN,Blevins J,Lou Y,Yang XF

    更新日期:2016-04-28 00:00:00