Abstract:
:A mutant of Escherichia coli resistant to serine hydroxamate which has a large increase in Km for serine of seryl-tRNA synthetase is described. The mutant serS gene was cloned and sequenced and was found to contain a single-base-pair mutation, resulting in the substitution of the residue alanine 262 by valine in motif 2. The methyl side chain of alanine 262 is not exposed at the active site, and molecular modeling indicated that replacement of alanine 262 by valine does not significantly affect the configuration of amino acids at the active site. This finding suggests that the residue at this position may be involved in a conformational change (possibly induced by ATP binding) which is necessary for optimal binding of the cognate amino acid.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Willison JC,Härtlein M,Leberman Rdoi
10.1128/jb.177.11.3347-3350.1995subject
Has Abstractpub_date
1995-06-01 00:00:00pages
3347-50issue
11eissn
0021-9193issn
1098-5530journal_volume
177pub_type
杂志文章abstract::The products of the bvgAS locus coordinately regulate expression of the Bordetella pertussis virulence regulon in response to environmental signals. Transcription of bvgAS-activated genes is nearly eliminated by several modulating conditions, including the presence of sulfate anion or nicotinic acid and growth at low ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.3.970-979.1992
更新日期:1992-02-01 00:00:00
abstract::Antigen 43 has been identified as a unique protein complex in the outer membrane of Escherichia coli. The complex contains two different polypeptides, alpha (Mr, 60,000) and beta (Mr, 53,000), in equal stoichiometry (P. Owen, P. Caffrey, and L.-G. Josefsson, J. Bacteriol. 169:3770-3777, 1987). The alpha subunit was re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.7.3634-3640.1989
更新日期:1989-07-01 00:00:00
abstract::Acetyl coenzyme A synthetase (Acs) activates acetate to acetyl coenzyme A through an acetyladenylate intermediate; two other enzymes, acetate kinase (Ack) and phosphotransacetylase (Pta), activate acetate through an acetyl phosphate intermediate. We subcloned acs, the Escherichia coli open reading frame purported to e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.10.2878-2886.1995
更新日期:1995-05-01 00:00:00
abstract::A clinical isolate of Staphylococcus aureus was found to be tolerant (MBC much greater than MIC) to a number of beta-lactam antibiotics, including oxacillin. Biophotometric analysis showed that a number of concentrations of oxacillin were capable of stimulating rapid cellular lysis in this organism, but the extent of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.4.1831-1836.1988
更新日期:1988-04-01 00:00:00
abstract::The two-component system SaeRS of Staphylococcus aureus is closely involved in the regulation of major virulence factors. However, little is known about the signals leading to saeRS activation. A total of four overlapping transcripts (T1 to T4) from three different transcription starting points are expressed in the sa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01927-07
更新日期:2008-05-01 00:00:00
abstract::Dworkin, Martin (Indiana University Medical Center, Indianapolis, Ind.). Nutritional requirements for vegetative growth of Myxococcus xanthus. J. Bacteriol. 84:250-257. 1962.-This investigation was part of a program to clarify the environmental regulation of fruiting-body formation in a fruiting myxobacterium. By use ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.2.250-257.1962
更新日期:1962-08-01 00:00:00
abstract::Cellulolytic strains of Bacillus stearothermophilus were isolated from nature and screened for the presence of activities associated with the degradation of plant cell walls. One isolate (strain XL-65-6) which exhibited strong activities with 4-methylumbelliferyl-beta-D-glucopyranoside (MUG) and 4-methylumbelliferyl-b...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.20.6441-6450.1993
更新日期:1993-10-01 00:00:00
abstract::Pigmentation mutants of Pseudomonas aeruginosa, selected by observed visual differences in coloration from the wild-type strain, were examined for altered patterns of phenazine synthesis. Three classes of mutants that were incapable of pyocyanine production were identified. Pigmentation patterns that were found to cha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.3.846-852.1979
更新日期:1979-06-01 00:00:00
abstract::Lornitzo, Frank A. (Veterans Administration Hospital, Madison, Wis.), and Dexter S. Goldman. Reversible effect of bicarbonate on the inhibition of mycobacterial and yeast transglucosylases by mycoribnin. J. Bacteriol. 89:1086-1091. 1965.-The transglucosylase which catalyzes the formation of trehalose-6-phosphate from ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.4.1086-1091.1965
更新日期:1965-04-01 00:00:00
abstract::When Bacillus megaterium spores germinate in the absence of an exogenous carbon source, the first minutes of germination are accompanied by production of large amounts (approximately 70 nmol/mg of dry spores) of acetate and much smaller amounts of pyruvate and lactate. The majority of these compounds are excreted into...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.132.2.744-746.1977
更新日期:1977-11-01 00:00:00
abstract::Strains of Escherichia coli K12 have been prepared that carry the ruv mutation in combination with lon or uvrA mutant alleles. The ruv minus uvrA minus double mutant is more sensitive to ultraviolet light than the urvA minus single mutant, whereas the strain with ruv and ion mutations shows the same sensitivity to ult...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.2.735-736.1975
更新日期:1975-02-01 00:00:00
abstract::A gene specifying the enzyme nicotinamide deamidase has been mapped, by the interrupted mating technique, at about 33 min on the chromosome of Escherichia coli K-12. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.101.3.1090-1091.1970
更新日期:1970-03-01 00:00:00
abstract::Features of the amino acid sequence of the TnaC nascent peptide are recognized by the translating ribosome. Recognition leads to tryptophan binding within the translating ribosome, inhibiting the termination of tnaC translation and preventing Rho-dependent transcription termination in the tna operon leader region. It ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01869-06
更新日期:2007-04-01 00:00:00
abstract::Binding of uncharged tRNA to the nascent transcript promotes readthrough of a leader region transcription termination signal in genes regulated by the T box transcription antitermination mechanism. Each gene in the T box family responds independently to its cognate tRNA, with specificity determined by base pairing of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.16.5392-5399.2004
更新日期:2004-08-01 00:00:00
abstract::The strong repression of inducible synthesis of the enzymes of fatty acid degradation by glucose can be partially relieved by the addition of cyclic adenosine 3',5' monophosphate (cyclic AMP) to the growth medium. This reversal of the glucose effect by cyclic AMP is not observed in a mutant (K29) that is unable to gro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.117.3.1178-1183.1974
更新日期:1974-03-01 00:00:00
abstract::We report on novel mutations in the malK gene of Salmonella enterica serovar Typhimurium, encoding the ATPase subunit of the maltose transporter (MalFGK(2)). Biochemical analysis suggests that (i) L86 might be involved in a signaling step during substrate translocation and (ii) E306 may be critical for the structural ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.5.1432-1436.2000
更新日期:2000-03-01 00:00:00
abstract::Ferric reductase activity was examined in Azotobacter vinelandii and was found to be located in the cytoplasm. The specific activities of soluble cell extracts were not affected by the iron concentration of the growth medium; however, activity was inhibited by the presence of Zn2+ during cell growth and also by the ad...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.7.4031-4037.1989
更新日期:1989-07-01 00:00:00
abstract::In wild-type Escherichia coli the activity of the maltose transport system is dependent on a periplasmic maltose-binding protein. It has been possible, however, to isolate mutants in which transport activity is mediated by the membrane components of the system and is no longer dependent on the periplasmic binding prot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.165.3.918-922.1986
更新日期:1986-03-01 00:00:00
abstract::pGC1, a self-transmissible lac plasmid that originated in Yersinia enterocolitica 842, is described. This plasmid is freely transmissible between strains of Y. enterocolitica and from such strains to strains of Escherichia coli. The plasmid can also be transferref among G. coli strains and back to Y. enterocolitica, a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.127.3.1058-1062.1976
更新日期:1976-09-01 00:00:00
abstract::Sixty-four mutants were isolated from the LT-2 wild-type strain of Salmonella typhimurium by selecting for chromate resistance. The majority of lesions were shown to lie in the cysA gene. (i) The mutants cannot take up sulfate, a finding which verifies the role of cysA in sulfate transport. In addition, 52 sulfate-tra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.3.1053-1062.1971
更新日期:1971-03-01 00:00:00
abstract::MreC and MreD, along with the actin homologue MreB, are required to maintain the shape of rod-shaped bacteria. The depletion of MreCD in rod-shaped bacteria leads to the formation of spherical cells and the accumulation of suppressor mutations. Ovococcus bacteria, such as Streptococcus pneumoniae, lack MreB homologues...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05245-11
更新日期:2011-08-01 00:00:00
abstract::An assay was developed to determine the amount of cyanophycin granules in blue-green algae. The amount of this polypeptide in cells of Anabaena cylindrica was measured as a function of culture age and was compared with changes in other proteinaceous cellular components. The data presented support the notion that the c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.3.1213-1216.1973
更新日期:1973-06-01 00:00:00
abstract::The coccoid cyanobacterium Chroococcidiopsis dominates microbial communities in the most extreme arid hot and cold deserts. These communities withstand constraints that result from multiple cycles of drying and wetting and/or prolonged desiccation, through mechanisms which remain poorly understood. Here we describe th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.7.2298-2305.2001
更新日期:2001-04-01 00:00:00
abstract::We screened Salmonella typhimurium, Citrobacter freundii, Klebsiella pneumoniae, Shigella boydii, and many isolates of Escherichia coli for DNA sequences homologous to those encoding each of two unrelated type I restriction and modification systems (EcoK and EcoA). Both K- and A-related hsd genes were identified, but ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.4.1775-1782.1988
更新日期:1988-04-01 00:00:00
abstract::A number of heterologous plasmid deoxyribonucleic acids (DNAs) coding for erythromycin, tylosin, lincomycin, tetracycline, or chloramphenicol resistance have been introduced into Streptococcus pneumoniae via genetic transformation with frequencies that varied between 10(-5) to as high as 5 x 10(-1) per colony-forming ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.2.698-709.1980
更新日期:1980-11-01 00:00:00
abstract::Thiobacillus neapolitanus, when grown in continuous culture with thiosulfate limiting growth, possessed an apparent maximal molar growth yield of 8.0 g (dry weight) per mole of thiosulfate. The substrate requirement for energy of maintenance was the highest yet reported, amounting to 21.8 mmoles of thiosulfate per g p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.3.874-878.1967
更新日期:1967-03-01 00:00:00
abstract::A rare group I intron in a cyanobacterial ribonucleotide reductase gene has been characterized. It contains a mobile insertion sequence element not required for RNA splicing. Ribonucleotide reductase genes were found to be hot spots for all three types of self-splicing intervening sequences, including group I and II i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01424-06
更新日期:2007-03-01 00:00:00
abstract::The DNA sequence of a 2,100-bp region containing the argE gene from Escherichia coli has been determined. The nucleotide sequence of the ppc-argE intergenic region was also solved and shown to contain six tandemly repeated REP sequences. Moreover, the oxyR gene has been mapped on the E. coli chromosome and shown to fl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.7.2323-2331.1992
更新日期:1992-04-01 00:00:00
abstract::Chemotaxis to the aromatic acid 4-hydroxybenzoate (4-HBA) by Pseudomonas putida is mediated by PcaK, a membrane-bound protein that also functions as a 4-HBA transporter. PcaK belongs to the major facilitator superfamily (MFS) of transport proteins, none of which have so far been implicated in chemotaxis. Work with two...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.16.5068-5074.1999
更新日期:1999-08-01 00:00:00
abstract::Adenosine 5'-triphosphate (ATP) synthesis driven by an artificially imposed membrane potential in right-side-out membrane vesicles of Escherichia coli was investigated. Membrane vesicles prepared in the presence of adenosine diphosphate were loaded with K+ by incubation with 0.5 M potassium phosphate. Addition of vali...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.127.1.154-161.1976
更新日期:1976-07-01 00:00:00