Variations in O-antigen biosynthesis and O-acetylation associated with altered phage sensitivity in Escherichia coli 4s.

abstract：:Evident in its name, the gastric pathogen Helicobacter pylori has a helical cell morphology which facilitates efficient colonization of the human stomach. An improved light-focusing strategy allowed us to robustly distinguish even subtle perturbations of H. pylori cell morphology by deviations in light-scattering prop...

journal_title：Journal of bacteriology

authors： Yang DC,Blair KM,Taylor JA,Petersen TW,Sessler T,Tull CM,Leverich CK,Collar AL,Wyckoff TJ,Biboy J,Vollmer W,Salama NR

更新日期：2019-06-21 00:00:00

abstract：:A novel mutant of the marine oil-degrading bacterium Alcanivorax borkumensis SK2, containing a mini-Tn5 transposon disrupting a "tesB-like" acyl-coenzyme A (CoA) thioesterase gene, was found to hyperproduce polyhydroxyalkanoates (PHA), resulting in the extracellular deposition of this biotechnologically important poly...

journal_title：Journal of bacteriology

authors： Sabirova JS,Ferrer M,Lünsdorf H,Wray V,Kalscheuer R,Steinbüchel A,Timmis KN,Golyshin PN

更新日期：2006-12-01 00:00:00

abstract：:The ompB operon of Escherichia coli contains the structural genes for two proteins, OmpR and EnvZ, which control the osmoregulated biosynthesis of the porin proteins OmpF and OmpC. By inserting XbaI octamer linkers into the cloned ompB locus, four distinct frameshift mutants were isolated and subsequently characterize...

journal_title：Journal of bacteriology

authors： Comeau DE,Ikenaka K,Tsung KL,Inouye M

更新日期：1985-11-01 00:00:00

abstract：:The presence of acetate and pyruvate groups in Klebsiella capsular polysaccharides may be demonstrated and estimated quantitatively by running the proton magnetic resonance spectrum of the polysaccharide (as sodium salt) in deuterium oxide at 95 C. Such spectra also permit an assessment to be made of the number of alp...

journal_title：Journal of bacteriology

authors： Bebault GM,Choy YM,Dutton GG,Funnell N,Stephen AM,Yang MT

更新日期：1973-03-01 00:00:00

abstract：:Paenibacillus mucilaginosus is a ubiquitous functional bacterium in microbial fertilizer. Here we report the complete sequence of P. mucilaginosus 3016. Multiple sets of functional genes have been found in the genome. To the best of our knowledge, this is the first announcement about the complete genome sequence of a ...

journal_title：Journal of bacteriology

authors： Ma M,Wang Z,Li L,Jiang X,Guan D,Cao F,Chen H,Wang X,Shen D,Du B,Li J

更新日期：2012-05-01 00:00:00

abstract：:Individually, the ribosomal proteins L1, L23, L36, and S6 are not essential for cell proliferation of Bacillus subtilis, but the absence of any one of these ribosomal proteins causes a defect in the formation of the 70S ribosomes and a reduced growth rate. In mutant strains individually lacking these ribosomal protein...

journal_title：Journal of bacteriology

authors： Akanuma G,Yamazaki K,Yagishi Y,Iizuka Y,Ishizuka M,Kawamura F,Kato-Yamada Y

更新日期：2018-08-24 00:00:00

abstract：:The β-barrel assembly machinery (BAM) complex is the core machinery for the assembly of β-barrel membrane proteins, and inhibition of BAM complex activity is lethal to bacteria. Discovery of integral membrane proteins that are key to pathogenesis and yet do not require assistance from the BAM complex raises the questi...

journal_title：Journal of bacteriology

authors： Hay ID,Belousoff MJ,Dunstan RA,Bamert RS,Lithgow T

更新日期：2018-02-07 00:00:00

abstract：:Transcriptional fusions to Salmonella typhimurium chromosomal genes were constructed by integration of a suicide fusion vector into the chromosome by homologous recombination with random cloned chromosomal fragments. We describe here a transductional method using the generalized transducing phage of S. typhimurium, P2...

journal_title：Journal of bacteriology

authors： Mahan MJ,Slauch JM,Mekalanos JJ

更新日期：1993-11-01 00:00:00

abstract：:Bacteria form communities, termed biofilms, in which cells adhere to each other within a matrix, typically comprised of polysaccharides, proteins, and extracellular DNA. Biofilm formation by the marine bacterium Vibrio fischeri requires the Syp polysaccharide, but the involvement of matrix proteins is as yet unknown. ...

journal_title：Journal of bacteriology

authors： Ray VA,Driks A,Visick KL

更新日期：2015-02-01 00:00:00

abstract：:We have constructed a promoter-probe expression vector for Bacillus subtilis. This plasmid, pCED6, can be used to fuse various DNA sequences to the structural gene of Escherichia coli beta-galactosidase, permitting analysis of the promoter activity of such sequences. pCED6 replicates and confers drug resistances in bo...

journal_title：Journal of bacteriology

authors： Donnelly CE,Sonenshein AL

更新日期：1984-03-01 00:00:00

abstract：:Acyl carrier protein was localized by immunoelectron microscopy in the cytoplasm of Escherichia coli. These data are inconsistent with the previous report of an association between acyl carrier protein and the inner membrane (H. Van den Bosch, J.R. Williamson, and P.R. Vagelos, Nature [London] 228:338-341, 1970). More...

journal_title：Journal of bacteriology

authors： Jackowski S,Edwards HH,Davis D,Rock CO

更新日期：1985-04-01 00:00:00

abstract：:The gram-positive bacterium Bacillus subtilis contains two minimal Tat translocases, TatAdCd and TatAyCy, which are each involved in the secretion of one or more specific protein substrates. We have investigated the subcellular localization of the TatA components by employing C-terminal green fluorescent protein (GFP)...

journal_title：Journal of bacteriology

authors： Ridder AN,de Jong EJ,Jongbloed JD,Kuipers OP

更新日期：2009-07-01 00:00:00

abstract：:Birnbaum, Jerome (University of Cincinnati, Cincinnati, Ohio), and Herman C. Lichstein. Metabolism of biotin and analogues of biotin by microorganisms. IV. Degradation of biotin, oxybiotin, and desthiobiotin by Lactobacillus casei. J. Bacteriol. 92:925-930. 1966.-Lactobacillus casei degrades biotin when it is present ...

journal_title：Journal of bacteriology

authors： Birnbaum J,Lichstein HC

更新日期：1966-10-01 00:00:00

abstract：:Phosphatidyl ethanolamine and lipopolysaccharide were extracted and purified from the cell envelope fractions of Escherichia coli and Salmonella typhimurium. The two components were studied separately and after recombination, by use of electron microscopy and monolayer techniques, and by measuring their ability to par...

journal_title：Journal of bacteriology

authors： Rothfield L,Horne RW

更新日期：1967-05-01 00:00:00

abstract：:The composition of the Pseudomonas solanacearum lipolysaccharide (LPS) was found to be similar to that described for the LPS of enterobacteria. The lipid A contained fatty acids and glucosamine in a molar ratio of 5:2. The LPS fraction contained 2-keto-3-deoxyoctulosonic acid, L-glycero-D-mannoheptose, hexoses (glucos...

journal_title：Journal of bacteriology

authors： Drigues P,Demery-Lafforgue D,Trigalet A,Dupin P,Samain D,Asselineau J

更新日期：1985-05-01 00:00:00

abstract：:Most bacteriophages (phages) release their progeny through the action of holins that form lesions in the cytoplasmic membrane and lysins that degrade the bacterial peptidoglycan. Although the function of each protein is well established in phages infecting Streptococcus pneumoniae, the role--if any--of the powerful ba...

journal_title：Journal of bacteriology

authors： Frias MJ,Melo-Cristino J,Ramirez M

更新日期：2009-09-01 00:00:00

abstract：:Escherichia coli B/r ATCC 12407 which grew synchronously with interdivision times of 45 and 85 min were exposed at intervals to chloramphenicol (200 mug/ml) and [(14)C]thymidine for short or long (residual) periods. The results suggested that the pattern of residual thymidine incorporation in 85-min cells yielded an e...

journal_title：Journal of bacteriology

authors： Helmstetter CE

更新日期：1974-10-01 00:00:00

abstract：:Oceaniovalibus guishaninsula, as a representative of a new genus within the family Rhodobacteraceae, was isolated from surface seawater that was sulfidic. Here, we present the draft genome sequence of the type strain, JLT2003(T). ...

journal_title：Journal of bacteriology

authors： Tang K,Liu K,Jiao N

更新日期：2012-12-01 00:00:00

abstract：:Wild-type Escherichia coli cannot grow on L-1,2-propanediol; mutants that can do so have increased basal activity of an NAD-linked L-1,2-propanediol oxidoreductase. This enzyme belongs to the L-fucose system and functions normally as L-lactaldehyde reductase during fermentation of the methylpentose. In wild-type cells...

journal_title：Journal of bacteriology

authors： Chen YM,Chakrabarti T,Lin EC

更新日期：1984-08-01 00:00:00

abstract：:In Escherichia coli K-12, expression of the lysU gene is regulated by the lrp gene product, as indicated by an increase in the level of lysyl-tRNA synthetase activity and LysU protein in an lrp mutant. Comparison of the patterns of protein expression visualized by two-dimensional gel electrophoresis indicated that Lys...

journal_title：Journal of bacteriology

authors： Lin R,Ernsting B,Hirshfield IN,Matthews RG,Neidhardt FC,Clark RL,Newman EB

更新日期：1992-05-01 00:00:00

abstract：:The activity of the toluene-responsive sigma(54) Pu promoter of the pWW0 TOL plasmid of Pseudomonas putida is down-regulated in vivo during exponential growth in rich medium and also by the presence of glucose in the culture. Although the Pu promoter already performs poorly during log growth in minimal medium when ame...

journal_title：Journal of bacteriology

authors： Cases I,de Lorenzo V

更新日期：2000-02-01 00:00:00

abstract：:Zymomonas mobilis ZM401 is a flocculating strain which can be self-immobilized within fermentors for a high-cell-density culture to improve ethanol productivity, as well as high-gravity fermentation to increase ethanol titer, due to its improved ethanol tolerance associated with the morphological change. Here, we repo...

journal_title：Journal of bacteriology

authors： Zhao N,Bai Y,Zhao XQ,Yang ZY,Bai FW

更新日期：2012-12-01 00:00:00

abstract：:Soluble c-type cytochromes are central to metabolism of C1 compounds in methylotrophic bacteria. In order to characterize the role of c-type cytochromes in methane-utilizing bacteria (methanotrophs), we have purified four different cytochromes, cytochromes c-554, c-553, c-552, and c-551, from the marine methanotroph M...

journal_title：Journal of bacteriology

authors： DiSpirito AA,Lipscomb JD,Lidstrom ME

更新日期：1990-09-01 00:00:00

abstract：:Proteins of the YidC/OxaI/Alb3 family play a crucial role in the insertion, folding, and/or assembly of membrane proteins in prokaryotes and eukaryotes. Bacillus subtilis has two YidC-like proteins, denoted SpoIIIJ and YqjG. SpoIIIJ and YqjG are largely exchangeable in function, but SpoIIIJ has a unique role in sporul...

journal_title：Journal of bacteriology

authors： Geng Y,de Keyzer J,Scheffers DJ,Driessen AJ

更新日期：2014-04-01 00:00:00

abstract：:We report the draft genome sequence of Lactobacillus salivarius SMXD51, isolated from the cecum of healthy chickens showing an activity against Campylobacter--the food-borne pathogen that is the most common cause of gastroenteritis in the European Union (EU)--and potentially interesting features for a probiotic strain...

journal_title：Journal of bacteriology

authors： Kergourlay G,Messaoudi S,Dousset X,Prévost H

更新日期：2012-06-01 00:00:00

abstract：:Riemerella anatipestifer is responsible for exudative septicemia in ducks. The genetic determinant of the CAMP cohemolysin, cam, from a strain of R. anatipestifer was cloned and expressed in Escherichia coli. Chromosomal DNA from serotype 19 strain 30/90 was used to construct a gene library in pBluescript II SK(-) vec...

journal_title：Journal of bacteriology

authors： Crasta KC,Chua KL,Subramaniam S,Frey J,Loh H,Tan HM

更新日期：2002-04-01 00:00:00

abstract：:T4 gene 2 mutants have a pleiotropic phenotype: degradation of injected phage DNA by exonuclease V (ExoV) in the recBCD(+) host cell cytoplasm and a low burst size due, at least in part, to a decreased ability for head-to-tail (H-T) joining. The more N terminal the mutation, the more pronounced is the H-T joining defe...

journal_title：Journal of bacteriology

authors： Wang GR,Vianelli A,Goldberg EB

更新日期：2000-02-01 00:00:00

abstract：:The physical arrangement of cell envelope components leads to the exposure of selected structural motifs which in turn may influence host-parasite interactions. To gain insight into the exposed epitopes, the present study describes a flow cytometric method designed to probe defined molecules on dispersed mycobacteria....

journal_title：Journal of bacteriology

authors： Ozanne V,Ortalo-Magne A,Vercellone A,Fournie JJ,Daffe M

更新日期：1996-12-01 00:00:00

abstract：:The SasG surface protein of Staphylococcus aureus has been shown to promote the formation of biofilm. SasG comprises an N-terminal A domain and repeated B domains. Here we demonstrate that SasG is involved in the accumulation phase of biofilm, a process that requires a physiological concentration of Zn(2+). The B doma...

journal_title：Journal of bacteriology

authors： Geoghegan JA,Corrigan RM,Gruszka DT,Speziale P,O'Gara JP,Potts JR,Foster TJ

更新日期：2010-11-01 00:00:00

abstract：:Dehalogenases are key enzymes in the metabolism of halo-organic compounds. This paper describes a systematic approach to the isolation and molecular analysis of two families of bacterial alpha-halocarboxylic acid (alphaHA) dehalogenase genes, called group I and group II deh genes. The two families are evolutionarily u...

journal_title：Journal of bacteriology

authors： Hill KE,Marchesi JR,Weightman AJ

更新日期：1999-04-01 00:00:00