Defining the region of Bacillus subtilis SpoIIIJ that is essential for its sporulation-specific function.

abstract：:Type III secretion systems (T3SSs) are essential virulence devices for many gram-negative bacteria that are pathogenic for plants, animals, and humans. They serve to translocate virulence effector proteins directly into eukaryotic host cells. T3SSs are composed of a large cytoplasmic bulb and a transmembrane region in...

journal_title：Journal of bacteriology

authors： Veenendaal AK,Sundin C,Blocker AJ

更新日期：2009-01-01 00:00:00

abstract：:FtsZ, the bacterial tubulin homologue, is the main player in at least two distinct processes of cell division during the development of Streptomyces coelicolor A3(2). It forms cytokinetic rings and is required for the formation of both the widely spaced hyphal cross walls in the substrate mycelium and the specialized ...

journal_title：Journal of bacteriology

authors： Grantcharova N,Lustig U,Flärdh K

更新日期：2005-05-01 00:00:00

abstract：:Campylobacter jejuni remains among the leading causes of bacterial food-borne illness. The current understanding of Campylobacter physiology suggests that it is asaccharolytic and is unable to catabolize exogenous carbohydrates. Contrary to this paradigm, we provide evidence for l-fucose utilization by C. jejuni. The ...

journal_title：Journal of bacteriology

authors： Muraoka WT,Zhang Q

更新日期：2011-03-01 00:00:00

abstract：:Xylella fastidiosa possesses both type I and type IV pili at the same cell pole. By use of a microfluidic device, the speed of twitching movement by wild-type cells on a glass surface against the flow direction of media was measured as 0.86 (standard error [SE], 0.04) microm min(-1). A type I pilus mutant (fimA) moved...

journal_title：Journal of bacteriology

authors： De La Fuente L,Burr TJ,Hoch HC

更新日期：2007-10-01 00:00:00

abstract：:Chlamydia spp. express a functional type III secretion system (T3SS) necessary for pathogenesis and intracellular growth. However, certain essential components of the secretion apparatus have diverged to such a degree as to preclude their identification by standard homology searches of primary protein sequences. One e...

journal_title：Journal of bacteriology

authors： Betts HJ,Twiggs LE,Sal MS,Wyrick PB,Fields KA

更新日期：2008-03-01 00:00:00

abstract：:High-density tiling microarray and RNA sequencing technologies were used to analyze the transcriptome of the periodontopathogenic bacterium Porphyromonas gingivalis. The compiled P. gingivalis transcriptome profiles were based on total RNA samples isolated from three different laboratory culturing conditions, and the ...

journal_title：Journal of bacteriology

authors： Høvik H,Yu WH,Olsen I,Chen T

更新日期：2012-01-01 00:00:00

abstract：:Siderophore-mediated iron transport in Pseudomonas aeruginosa is dependent upon the cytoplasmic membrane-associated TonB1 energy coupling protein for activity. To assess the functional significance of the various regions of this molecule and to identify functionally important residues, the tonB1 gene was subjected to ...

journal_title：Journal of bacteriology

authors： Zhao Q,Poole K

更新日期：2002-03-01 00:00:00

abstract：:In response to DNA damage or the inhibition of normal DNA replication in Escherichia coli, a set of some 20 unlinked operons is induced through the RecA-mediated cleavage of the LexA repressor. We examined the effect of this SOS response on the transposition of Tn5 and determined that the frequency of transposition is...

journal_title：Journal of bacteriology

authors： Weinreich MD,Makris JC,Reznikoff WS

更新日期：1991-11-01 00:00:00

abstract：:A Pseudomonas strain produced an enzyme capable of deacylating 7 beta-(4-carboxybutanamido)cephalosporanic acid to 7-aminocephalosporanic acid in response to glutaric acid. The gene for the enzyme was cloned within the PstI site of pBR325 as a 7.35-kilobase-pair DNA segment from a mutant of this strain whose enzyme is...

journal_title：Journal of bacteriology

authors： Matsuda A,Komatsu KI

更新日期：1985-09-01 00:00:00

abstract：:After heating at 65 C, ribosomes isolated from Bacillus stearothermophilus were strikingly more heat-stable than comparable preparations from Escherichia coli when tested for ability to support polyuridylic acid-directed phenylalanine incorporation at 37 C. The stability of ribosomes was also determined by measurement...

journal_title：Journal of bacteriology

authors： Friedman SM,Axel R,Weinstein IB

更新日期：1967-05-01 00:00:00

abstract：:The ability to restart broken DNA replication forks is essential across all domains of life. In Escherichia coli, the priA, priB, priC, and dnaT genes encode the replication restart proteins (RRPs) to accomplish this task. PriA plays a critical role in replication restart such that its absence reveals a dramatic pheno...

journal_title：Journal of bacteriology

authors： Leroux M,Jani N,Sandler SJ

更新日期：2017-08-08 00:00:00

abstract：:Mevalonate (MVA) metabolism provides the isoprenoids used in archaeal lipid biosynthesis. In synthesis of isopentenyl diphosphate, the classical MVA pathway involves decarboxylation of mevalonate diphosphate, while an alternate pathway has been proposed to involve decarboxylation of mevalonate monophosphate. To identi...

journal_title：Journal of bacteriology

authors： Vannice JC,Skaff DA,Keightley A,Addo JK,Wyckoff GJ,Miziorko HM

更新日期：2014-03-01 00:00:00

abstract：:Mycobacterium tuberculosis has a clonal population structure, and the Latin American-Mediterranean (LAM) family is one of the largest and most widespread within this species, showing evidence for remarkable pathobiology and a confusing phylogeny. Here, we applied robust phylogenetic markers to study the evolution of t...

journal_title：Journal of bacteriology

authors： Mokrousov I,Vyazovaya A,Narvskaya O

更新日期：2014-05-01 00:00:00

abstract：:Aeromonas salmonicida subsp. salmonicida, the etiological agent of furunculosis, is an important fish pathogen. We have screened this bacterium with a broad-host-range probe directed against yscV, the gene that encodes the archetype of a highly conserved family of inner membrane proteins found in every known type III ...

journal_title：Journal of bacteriology

authors： Burr SE,Stuber K,Wahli T,Frey J

更新日期：2002-11-01 00:00:00

abstract：:In order to clarify the relationship between polyploidization and the capability of phenotypic switching in the imperfect yeast Candida albicans, two types of variants were isolated as segregants from a fusant, which produced a proportion of the cell population with a higher ploidy than the rest, either in a temperatu...

journal_title：Journal of bacteriology

authors： Suzuki T,Hitomi A,Magee PT,Sakaguchi S

更新日期：1994-06-01 00:00:00

abstract：:Nε-lysine acetylation was recently discovered on many bacterial proteins that function in diverse cellular processes. Thus, many questions remain unanswered. For example, what mechanisms regulate lysine acetylation? Does acetylation affect physiology? To help answer these questions, we studied the Escherichia coli res...

journal_title：Journal of bacteriology

authors： Hu LI,Chi BK,Kuhn ML,Filippova EV,Walker-Peddakotla AJ,Bäsell K,Becher D,Anderson WF,Antelmann H,Wolfe AJ

更新日期：2013-09-01 00:00:00

abstract：:Succinyl-coenzyme A synthetase of Escherichia coli has an alpha 2 beta 2 subunit structure. By measuring reconstituted enzyme activity present after addition of purified alpha or beta subunits to cell extracts followed by refolding, we have shown that extracts contain no significant excess of either subunit species. T...

journal_title：Journal of bacteriology

authors： Wolodko WT,Brownie ER,Bridger WA

更新日期：1980-07-01 00:00:00

abstract：:Spheroplasts of two different auxotrophic strains of Saccharomyces cerevisiae, both of mating type a, were fused with the aid of polyethylene glycol and calcium ions. After reversion to vegetative cells in solid media, the resulting zygotes were shown to be diploid cells of mating type aa. ...

journal_title：Journal of bacteriology

authors： van Solingen P,van der Plaat JB

更新日期：1977-05-01 00:00:00

abstract：:Henle, Gertrude (Children's Hospital of Philadelphia, Philadelphia, Pa.), and Werner Henle. Immunofluorescence in cells derived from Burkitt's lymphoma. J. Bacteriol. 91:1248-1256. 1966.-Indirect immunofluorescence tests led to the brilliant staining of a small proportion of the cells in five different cultures derive...

journal_title：Journal of bacteriology

authors： Henle G,Henle W

更新日期：1966-03-01 00:00:00

abstract：:A comparison of the distribution of CO oxidation activity between soluble and particulate protein fractions obtained after disruption of CO-grown Rhodopseudomonas gelatinosa 1 by French pressure cell breakage and osmotic lysis of spheroplasts suggested that, in situ, the enzyme complex was associated with the cell mem...

journal_title：Journal of bacteriology

authors： Wakim BT,Uffen RL

更新日期：1983-01-01 00:00:00

abstract：:Moorella thermoacetica can grow with H₂ and CO₂, forming acetic acid from 2 CO₂ via the Wood-Ljungdahl pathway. All enzymes involved in this pathway have been characterized to date, except for methylenetetrahydrofolate reductase (MetF). We report here that the M. thermoacetica gene that putatively encodes this enzyme,...

journal_title：Journal of bacteriology

authors： Mock J,Wang S,Huang H,Kahnt J,Thauer RK

更新日期：2014-09-01 00:00:00

abstract：:Evident in its name, the gastric pathogen Helicobacter pylori has a helical cell morphology which facilitates efficient colonization of the human stomach. An improved light-focusing strategy allowed us to robustly distinguish even subtle perturbations of H. pylori cell morphology by deviations in light-scattering prop...

journal_title：Journal of bacteriology

authors： Yang DC,Blair KM,Taylor JA,Petersen TW,Sessler T,Tull CM,Leverich CK,Collar AL,Wyckoff TJ,Biboy J,Vollmer W,Salama NR

更新日期：2019-06-21 00:00:00

abstract：:Partial denaturation of Saccharomyces cerevisiae chromosomal DNA was found to occur spontaneously during meiosis. Short regions of strand separation (300 base pairs long) were seen in DNA molecules prepared for electron microscopy by the aqueous spreading technique. These regions were clustered along the DNA. The time...

journal_title：Journal of bacteriology

authors： Klein HL,Byers B

更新日期：1978-05-01 00:00:00

abstract：:Biochemical events which occur during macroconidial germination have been studied in the dermatophyte Microsporum gypseum. The specific activity levels of various metabolic enzymes have been assayed during germination time periods. The accumulated levels of several of these enzymes, as a function of exogenous carbohyd...

journal_title：Journal of bacteriology

authors： Leighton TJ,Stock JJ,Kelln RA

更新日期：1970-08-01 00:00:00

abstract：:We have overexpressed and purified the Helicobacter pylori Fur protein and analyzed its interaction with the intergenic regions of divergent genes involved in iron uptake (frpB and ceuE) and oxygen radical detoxification (katA and tsaA). DNase I footprint analysis showed that Fur binds specifically to a high-affinity ...

journal_title：Journal of bacteriology

authors： Delany I,Pacheco AB,Spohn G,Rappuoli R,Scarlato V

更新日期：2001-08-01 00:00:00

abstract：:A gene (yacK) encoding a putative multicopper oxidase (MCO) was cloned from Escherichia coli, and the expressed enzyme was demonstrated to exhibit phenoloxidase and ferroxidase activities. The purified protein contained six copper atoms per polypeptide chain and displayed optical and electron paramagnetic resonance (E...

journal_title：Journal of bacteriology

authors： Kim C,Lorenz WW,Hoopes JT,Dean JF

更新日期：2001-08-01 00:00:00

abstract：:The genetic organization of the foc gene cluster has been studied; six genes involved in the biogenesis of F1C fimbriae were identified. focA encodes the major fimbrial subunit, focC encodes a product that is indispensable for fimbria formation, focG and focH encode minor fimbrial subunits, and focI encodes a protein ...

journal_title：Journal of bacteriology

authors： Riegman N,Kusters R,Van Veggel H,Bergmans H,Van Bergen en Henegouwen P,Hacker J,Van Die I

更新日期：1990-02-01 00:00:00

abstract：:The impact of arsenite [As(III)] on several levels of cellular metabolism and gene regulation was examined in Pseudomonas aeruginosa. P. aeruginosa isogenic mutants devoid of antioxidant enzymes or defective in various metabolic pathways, DNA repair systems, metal storage proteins, global regulators, or quorum sensing...

journal_title：Journal of bacteriology

authors： Parvatiyar K,Alsabbagh EM,Ochsner UA,Stegemeyer MA,Smulian AG,Hwang SH,Jackson CR,McDermott TR,Hassett DJ

更新日期：2005-07-01 00:00:00

abstract：:The glutamate permeation system in Escherichia coli K-12 consists of three genes: gltC, gltS, and gltR. The genes gltC and gltS are very closely linked, and are located between the pyrE and tna loci, in the following order: tna, gltC, gltS, pyrE; gltR is located near the metA gene. The three glt genes constitute a reg...

journal_title：Journal of bacteriology

authors： Marcus M,Halpern YS

更新日期：1969-03-01 00:00:00

abstract：:Three species of Desulfovibrio were found to have a high degree of ribosomal ribonucleic acid homology with Desulfovibrio vulgaris. Desulfotomaculum nigrificans, which is also a sulfate-reducing anaerobe, had only 38% ribosomal ribonucleic acid homology with D. vulgaris. The homologies of six other unrelated genera we...

journal_title：Journal of bacteriology

authors： Pace B,Campbell LL

更新日期：1971-06-01 00:00:00