Genetic analysis of the glutamate permease in Escherichia coli K-12.

Abstract:

:The glutamate permeation system in Escherichia coli K-12 consists of three genes: gltC, gltS, and gltR. The genes gltC and gltS are very closely linked, and are located between the pyrE and tna loci, in the following order: tna, gltC, gltS, pyrE; gltR is located near the metA gene. The three glt genes constitute a regulatory system in which gltR is the regulator gene responsible for the formation of repressor, gltS is the structural gene of the glutamate permease, and gltC is most probably the operator locus. The synthesis of glutamate permease is partially repressed in wild-type K-12 strains, resulting in the inability of these strains to utilize glutamate as the sole source of carbon. Derepression due to mutation at the gltC locus enables growth on glutamate as a carbon source both at 30 C and at 42 C. Temperature-sensitive gltR mutants capable of utilizing glutamate for growth at 42 C but not at 30 C were found to be derepressed for glutamate permease when grown at 42 C and partially repressed (wild-type phenotype) upon growth at 30 C. These mutants produce an altered thermolabile repressor which can be inactivated by mild heat treatment (10 min at 44 C) in the absence of growth.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Marcus M,Halpern YS

doi

10.1128/JB.97.3.1118-1128.1969

subject

Has Abstract

pub_date

1969-03-01 00:00:00

pages

1118-28

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

97

pub_type

杂志文章
  • Compensatory functions of two alkyl hydroperoxide reductases in the oxidative defense system of Legionella pneumophila.

    abstract::Legionella pneumophila expresses two catalase-peroxidase enzymes that exhibit strong peroxidatic but weak catalatic activities, suggesting that other enzymes participate in decomposition of hydrogen peroxide (H2O2). Comparative genomics revealed that L. pneumophila and its close relative Coxiella burnetii each contain...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00635-06

    authors: LeBlanc JJ,Davidson RJ,Hoffman PS

    更新日期:2006-09-01 00:00:00

  • Site-directed mutagenesis in Escherichia coli of a stable R772::Ti cointegrate plasmid from Agrobacterium tumefaciens.

    abstract::The host range of an octopine Ti plasmid is limited to Rhizobiaceae. This has been extended also to Escherichia coli in the form of a stable cointegrate with the wide-host-range plasmid R772. Its structure was studied by constructing a physical map of R772 and of the R772::pTiB6 cointegrate. An insertion sequence pres...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.154.2.693-701.1983

    authors: Hille J,van Kan J,Klasen I,Schilperoort R

    更新日期:1983-05-01 00:00:00

  • Role of PBP1 in cell division of Staphylococcus aureus.

    abstract::We constructed a conditional mutant of pbpA in which transcription of the gene was placed under the control of an IPTG (isopropyl-beta-D-thiogalactopyranoside)-inducible promoter in order to explore the role of PBP1 in growth, cell wall structure, and cell division. A methicillin-resistant strain and an isogenic methi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00044-07

    authors: Pereira SF,Henriques AO,Pinho MG,de Lencastre H,Tomasz A

    更新日期:2007-05-01 00:00:00

  • Oxygen-insensitive nitroreductases: analysis of the roles of nfsA and nfsB in development of resistance to 5-nitrofuran derivatives in Escherichia coli.

    abstract::Nitroheterocyclic and nitroaromatic compounds constitute an enormous range of chemicals whose potent biological activity has significant human health and environmental implications. The biological activity of nitro-substituted compounds is derived from reductive metabolism of the nitro moiety, a process catalyzed by a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.21.5529-5539.1998

    authors: Whiteway J,Koziarz P,Veall J,Sandhu N,Kumar P,Hoecher B,Lambert IB

    更新日期:1998-11-01 00:00:00

  • Salmonella typhimurium cobalamin (vitamin B12) biosynthetic genes: functional studies in S. typhimurium and Escherichia coli.

    abstract::In order to study the Salmonella typhimurium cobalamin biosynthetic pathway, the S. typhimurium cob operon was isolated and cloned into Escherichia coli. This approach has given the new host of the cob operon the ability to make cobalamins de novo, an ability that had probably been lost by this organism. In total, 20 ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.3.753-767.1996

    authors: Raux E,Lanois A,Levillayer F,Warren MJ,Brody E,Rambach A,Thermes C

    更新日期:1996-02-01 00:00:00

  • Identification and characterization of plasmids from the western aster yellows mycoplasmalike organism.

    abstract::Supercoiled double-stranded DNA molecules (plasmids) were isolated from plants infected with three laboratory strains of western aster yellows mycoplasma-like organism (AY-MLO) by using cesium chloride-ethidium bromide density gradients. Southern blot analysis, using plasmids from the severe strain of AY-MLO (SAY-MLO)...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.3.1628-1633.1990

    authors: Kuske CR,Kirkpatrick BC

    更新日期:1990-03-01 00:00:00

  • Genetic analysis of pathway specificity during posttranslational protein translocation across the Escherichia coli plasma membrane.

    abstract::In Escherichia coli, the SecB/SecA branch of the Sec pathway and the twin-arginine translocation (Tat) pathway represent two alternative possibilities for posttranslational translocation of proteins across the cytoplasmic membrane. Maintenance of pathway specificity was analyzed using a model precursor consisting of t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.9.2811-2819.2003

    authors: Blaudeck N,Kreutzenbeck P,Freudl R,Sprenger GA

    更新日期:2003-05-01 00:00:00

  • Changes in cyclic nucleotide levels and dimorphic transition in Candida albicans.

    abstract::The relationship between the levels of cyclic nucleotides and dimorphic transition in Candida albicans was examined. The results showed that cells of this pathogenic fungus contained both cyclic adenosine 3',5'-monophosphate (cAMP) and cyclic guanosine 3',5'-monophosphate (cGMP), the concentration of the latter being ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.142.3.1010-1014.1980

    authors: Niimi M,Niimi K,Tokunaga J,Nakayama H

    更新日期:1980-06-01 00:00:00

  • Identification of long intergenic repeat sequences associated with DNA methylation sites in Caulobacter crescentus and other alpha-proteobacteria.

    abstract::A systematic search for motifs associated with CcrM DNA methylation sites revealed four long (>100-bp) motifs (CIR sequences) present in up to 21 copies in Caulobacter crescentus. The CIR1 and CIR2 motifs exhibit a conserved inverted repeat organization, with a CcrM site in the center of one of the repeats. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.16.4997-5002.2003

    authors: Chen SL,Shapiro L

    更新日期:2003-08-01 00:00:00

  • Inhibitory action of selenite on Escherichia coli, Proteus vulgaris, and Salmonella thompson.

    abstract::Weiss, K. F. (Iowa State University, Ames), J. C. Ayres, and A. A. Kraft. Inhibitory action of selenite on Escherichia coli, Proteus vulgaris, and Salmonella thompson. J. Bacteriol. 90:857-862. 1965.-The resistance of three microorganisms, Escherichia coli (ISU-41), Proteus vulgaris (ISU-37c), and Salmonella thompson ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.4.857-862.1965

    authors: Weiss KF,Ayres JC,Kraft AA

    更新日期:1965-10-01 00:00:00

  • Localization of enzymes in Ureaplasma urealyticum (T-strain mycoplasma).

    abstract::Ureaplasma urealyticum cells were lysed by osmotic shock or by digitonin. The membrane fraction contained four to ten times as much protein as the cytoplasmic fraction. These values are in large excess of those reported for classical mycoplasmas, suggesting that the Ureaplasma membrane fraction was heavily contaminate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.1.297-302.1977

    authors: Masover GK,Razin S,Hayflick L

    更新日期:1977-04-01 00:00:00

  • Phosphoinositides are involved in control of the glucose-dependent growth resumption that follows the transition phase in Streptomyces lividans.

    abstract::The interruption of the sblA gene of Streptomyces lividans was previously shown to lead to relief of glucose repression of the normally strongly glucose-repressed alpha-amylase gene. In addition to this relief, an early entry into stationary phase was observed when cells were grown in a minimal medium containing gluco...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00891-06

    authors: Chouayekh H,Nothaft H,Delaunay S,Linder M,Payrastre B,Seghezzi N,Titgemeyer F,Virolle MJ

    更新日期:2007-02-01 00:00:00

  • Cell division in Escherichia coli BS-12 is hypersensitive to deoxyribonucleic acid damage by ultraviolet light.

    abstract::Escherichia coli BS-12 uvrA lon is hypersensitive to ultraviolet light. On minimal agar plates at densities in excess of about 10(7) bacteria per plate, as few as one or two photoreversible pyrimidine dimers in the entire genome are sufficient to cause inhibition of cell division. Most of the resulting filaments are u...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.2.724-728.1977

    authors: Bridges BA,Mottershead RP,Green MH

    更新日期:1977-05-01 00:00:00

  • Patterns of protein production in Myxococcus xanthus during spore formation induced by glycerol, dimethyl sulfoxide, and phenethyl alcohol.

    abstract::Spore formation of Myxococcus xanthus can occur not only on agar plates during fruiting body formation, but also in a liquid culture by simply adding glycerol, dimethyl sulfoxide, or phenethyl alcohol to the culture. This chemically-induced spore formation occurs synchronously and much faster than that occurring durin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.144.3.1076-1082.1980

    authors: Komano T,Inouye S,Inouye M

    更新日期:1980-12-01 00:00:00

  • Evidence for the Translocation of a Chromosome Sement in Bacillus subtilis Strains Carrying the trpE26 Mutation.

    abstract::The replication order of markers was studied in Bacillus subtilis strains bearing the trpE26 mutation by the use of the density transfer technique. An important difference in this order was observed in comparison with that of strain 168 T-. All markers tested of a chromosome segment extending from trpD to ilvA replica...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.122.3.886-898.1975

    authors: Trowsdale J,Anagnostopoulos C

    更新日期:1975-06-01 00:00:00

  • Transcription of spontaneously released bacterial deoxyribonucleic acid in frog auricles.

    abstract::After frog auricles have been in contact with a suspension of bacteria or bacteria-free supernatant fluid, newly synthesized bacterial ribonucleic acid (RNA) is recovered in animal cells. It appears that the presence of bacterial deoxyribonucleic acid (DNA)-dependent RNA polymerase is necessary for the transcription o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.114.1.114-120.1973

    authors: Stroun M,Anker P

    更新日期:1973-04-01 00:00:00

  • Coexpression of Escherichia coli obgE, Encoding the Evolutionarily Conserved Obg GTPase, with Ribosomal Proteins L21 and L27.

    abstract:UNLABELLED:Multiple essential small GTPases are involved in the assembly of the ribosome or in the control of its activity. Among them, ObgE (CgtA) has been shown recently to act as a ribosome antiassociation factor that binds to ppGpp, a regulator whose best-known target is RNA polymerase. The present study was aimed ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00159-16

    authors: Maouche R,Burgos HL,My L,Viala JP,Gourse RL,Bouveret E

    更新日期:2016-06-13 00:00:00

  • Bacillus subtilis pellicle formation proceeds through genetically defined morphological changes.

    abstract::Biofilms are structured multicellular communities of bacteria that form through a developmental process. In standing culture, undomesticated strains of Bacillus subtilis produce a floating biofilm, called a pellicle, with a distinct macroscopic architecture. Here we report on a comprehensive analysis of B. subtilis pe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00157-07

    authors: Kobayashi K

    更新日期:2007-07-01 00:00:00

  • Quantifying aggregation dynamics during Myxococcus xanthus development.

    abstract::Under starvation conditions, a swarm of Myxococcus xanthus cells will undergo development, a multicellular process culminating in the formation of many aggregates called fruiting bodies, each of which contains up to 100,000 spores. The mechanics of symmetry breaking and the self-organization of cells into fruiting bod...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05188-11

    authors: Zhang H,Angus S,Tran M,Xie C,Igoshin OA,Welch RD

    更新日期:2011-10-01 00:00:00

  • Molecular cloning of genes involved with expression of A-band lipopolysaccharide, an antigenically conserved form, in Pseudomonas aeruginosa.

    abstract::Most strains of Pseudomonas aeruginosa can express two chemically and immunologically distinct types of lipopolysaccharide (LPS), an antigenically conserved form called A band and the serotype-specific form called B band. To study the molecular controls regulating expression of the A-band LPS antigen, we have cloned t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.18.5624-5630.1991

    authors: Lightfoot J,Lam JS

    更新日期:1991-09-01 00:00:00

  • EFFECTS OF BICARBONATE ON GROWTH OF PASTEURELLA PESTIS. I. DIFFERENTIAL RESPONSE OF VIRULENT AND AVIRULENT CELLS.

    abstract::Surgalla, M. J. (Fort Detrick, Frederick, Md.), A. W. Andrews, and C. L. Baugh. Effects of bicarbonate on growth of Pasteurella pestis. I. Differential response of virulent and avirulent cells. J. Bacteriol. 88:269-272. 1964.-Virulent Pasteurella pestis and the avirulent mutants always present in a virulent inoculum h...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.2.269-272.1964

    authors: SURGALLA MJ,ANDREWS AW,BAUGH CL

    更新日期:1964-08-01 00:00:00

  • A novel apical corpuscle in hypahe of Mucor rouxii.

    abstract::Median longitudinal sections of germ tube apices of Mucor rouxii revealed the presence of a single, roughly hemispherical, electron-dense organelle, in intimate contact with the apical cell wall. Conceivably, this "apical corpuscle" may be responsible for the emission of the germ tube and its continued apical growth. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.6.2399-2402.1968

    authors: Bartnicki-Garcia S,Nelson N,Cota-Robles E

    更新日期:1968-06-01 00:00:00

  • Deletion analysis of the carboxyl-terminal region of the PomB component of the vibrio alginolyticus polar flagellar motor.

    abstract::The stator of the sodium-driven flagellar motor of Vibrio alginolyticus is a membrane protein complex composed of four PomA and two PomB subunits. PomB has a peptidoglycan-binding motif in the C-terminal region. In this study, four kinds of PomB deletions in the C terminus were constructed. None of the deletion protei...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.2.778-784.2005

    authors: Yakushi T,Hattori N,Homma M

    更新日期:2005-01-01 00:00:00

  • Hexuronic acid dehydrogenase of Agrobacterium tumefaciens.

    abstract::Growth of Agrobacterium tumefaciens on d-glucuronic acid (GlcUA) or d-galacturonic acid (GalUA) induces formation of hexuronic acid dehydrogenase [d-aldohexuronic acid: nicotinamide adenine dinucleotide (NAD) oxidoreductase]. The dehydrogenase, which irreversibly converts GlcUA or GalUA to the corresponding hexaric ac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.99.3.667-673.1969

    authors: Chang YF,Feingold DS

    更新日期:1969-09-01 00:00:00

  • Quantitative assessment of oxygen availability: perceived aerobiosis and its effect on flux distribution in the respiratory chain of Escherichia coli.

    abstract::Despite a large number of studies on the role of oxygen in cellular processes, there is no consensus as to how oxygen availability to the cell should be defined, let alone how it should be quantified. Here, a quantitative definition for oxygen availability (perceived aerobiosis) is presented; the definition is based o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.5.1402-1406.2002

    authors: Alexeeva S,Hellingwerf KJ,Teixeira de Mattos MJ

    更新日期:2002-03-01 00:00:00

  • Autoplaquing in Myxococcus strains.

    abstract::Autoplaquing has been observed in Myxococcus strains freshly isolated from soil. Initial observations suggest that this phenomenon is not induced by elevated temperature or visible light; we suggest that it may be the result of a derangement in the developmental autolytic mechanism within the cell. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.158.3.1163-1164.1984

    authors: Breyen SA,Dworkin M

    更新日期:1984-06-01 00:00:00

  • Genetic analysis of the O-specific lipopolysaccharide biosynthesis region (rfb) of Escherichia coli K-12 W3110: identification of genes that confer group 6 specificity to Shigella flexneri serotypes Y and 4a.

    abstract::We recently reported a novel genetic locus located in the sbcB-his region of the chromosomal map of Escherichia coli K-12 which directs the expression of group 6-positive phenotype in Shigella flexneri lipopolysaccharide, presumably due to the transfer of O-acetyl groups onto rhamnose residues of the S. flexneri O-spe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.13.4133-4143.1994

    authors: Yao Z,Valvano MA

    更新日期:1994-07-01 00:00:00

  • Ultrastructural studies on the yeastlike and mycelial phases of Sporotrichum schenckii.

    abstract::Fine details of the internal and external morphology of the yeastlike and mycelial phases of the dimorphic fungal pathogen Sporotrichum schenckii as seen in ultrathin sections are described and illustrated by electronphotomicrography. Comparisons of yeastlike phase ultrastructure were made using two different methods ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.2.1010-1019.1969

    authors: Lane JW,Grrison RG,Field MF

    更新日期:1969-11-01 00:00:00

  • Rapid transient growth at low pH in the cyanobacterium Synechococcus sp.

    abstract::The thermophilic cyanobacterium Synechococcus sp. strain Y-7c-s grows at its maximum rate at a high pH (pH 8 and above) the does not show sustained growth below pH 6.5. However, rapidly growing, exponential-phase cells from high-pH cultures continued to grow rapidly for several hours after transfer to pH 6.0 or 5.0. T...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.149.1.237-246.1982

    authors: Kallas T,Castenholz RW

    更新日期:1982-01-01 00:00:00

  • Anaerobic degradation of the benzene nucleus by a facultatively anaerobic microorganism.

    abstract::A bacterium was isolated by elective culture with p-hydroxybenzoate as substrate and nitrate as electron acceptor. It grew either aerobically or anaerobically, by nitrate respiration, on a range of aromatic compounds. The organism was identified as a pseudomonad and was given the trivial name Pseudomonas PN-1. Benzoat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.102.2.430-437.1970

    authors: Taylor BF,Campbell WL,Chinoy I

    更新日期:1970-05-01 00:00:00