Deletion analysis of the carboxyl-terminal region of the PomB component of the vibrio alginolyticus polar flagellar motor.

Abstract:

:The stator of the sodium-driven flagellar motor of Vibrio alginolyticus is a membrane protein complex composed of four PomA and two PomB subunits. PomB has a peptidoglycan-binding motif in the C-terminal region. In this study, four kinds of PomB deletions in the C terminus were constructed. None of the deletion proteins restored motility of the DeltapomB strain. The PomA protein was coisolated with all of the PomB derivatives under detergent-solubilized conditions. Homotypic disulfide cross-linking of all of the deletion derivatives through naturally occurring Cys residues was detected. We conclude that the C-terminal region of PomB is essential for motor function but not for oligomerization of PomB with itself or PomA.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Yakushi T,Hattori N,Homma M

doi

10.1128/JB.187.2.778-784.2005

keywords:

subject

Has Abstract

pub_date

2005-01-01 00:00:00

pages

778-84

issue

2

eissn

0021-9193

issn

1098-5530

pii

187/2/778

journal_volume

187

pub_type

杂志文章
  • Localization of acid phosphatase activity in the basidia of Coprinus micaceus.

    abstract::Gill lamellae from the young fruiting bodies of the basidiomycete Coprinus micaceus possess cytoplasmic particles with cytochemically demonstrable acid phosphatase activity; they are presumed to be lysosomes. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.115.1.443-446.1973

    authors: Nehemiah JL

    更新日期:1973-07-01 00:00:00

  • Genes for the establishment and maintenance of lysogeny by the temperate coliphage 186.

    abstract::To identify the genes in coliphage 186 that are required for lysogeny, we isolated clear-plaque mutants. Complementation studies and DNA sequencing identified two genes, the cI gene for the immunity maintenance repressor and the cII gene, which is required only for the establishment of lysogeny. One mutant carried a c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.16.5286-5288.1993

    authors: Lamont I,Richardson H,Carter DR,Egan JB

    更新日期:1993-08-01 00:00:00

  • Mechanistically novel iron(III) transport system in Serratia marcescens.

    abstract::A novel iron(III) transport system of Serratia marcescens, named SFU, was cloned and characterized in Escherichia coli. Iron acquisition by this system differed from that by E. coli and related organisms. No siderophore production and no receptor protein related to the SFU system could be detected. In addition, iron u...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.1.238-243.1989

    authors: Zimmermann L,Angerer A,Braun V

    更新日期:1989-01-01 00:00:00

  • Use of temperature-sensitive mutants to study gene expression during sporulation in Bacillus subtilis.

    abstract::Two temperature-sensitive sporulation mutants have been characterized. One mutant, which is blocked at stage II, has a short temperature-sensitive period that occurs at about the time when the spore septum is formed. Cells can escape the sporulation block, if incubated for a short period at the permissive temperature,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.126.2.928-936.1976

    authors: Young M

    更新日期:1976-05-01 00:00:00

  • Isoleucine auxotrophy as a consequence of a mutationally altered isoleucyl-transfer ribonucleic acid synthetase.

    abstract::Among mutants which require isoleucine, but not valine, for growth, we have found two distinguishable classes. One is defective in the biosynthetic enzyme threonine deaminase (l-threonine hydro-lyase, deaminating, EC 4.2.1.16) and the other has an altered isoleucyl transfer ribonucleic acid (tRNA) synthetase [l-isoleu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.2.527-537.1971

    authors: Iaccarino M,Berg P

    更新日期:1971-02-01 00:00:00

  • The Sakaguchi reaction product quenches phycobilisome fluorescence, allowing determination of the arginine concentration in cells of Anabaena strain PCC 7120.

    abstract::The filamentous cyanobacterium Anabaena fixes nitrogen in specialized cells called heterocysts. The immediate product of fixation, ammonia, is known to be assimilated by addition to glutamate to make glutamine. How fixed nitrogen is transported along the filament to the 10 to 20 vegetative cells that separate heterocy...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01512-12

    authors: Ke S,Haselkorn R

    更新日期:2013-01-01 00:00:00

  • Lactate dehydrogenase activity in certain strains of Staphylococcus aureus.

    abstract::Lactate dehydrogenase (LDH) was studied in phage-propagating strains 29, 3A, 6, 81, and 42D of Staphylococcus aureus selected from the five groups in the International-Blair series. Cells were cultivated in Brain Heart Infusion (Difco) under nearly anaerobic conditions and were harvested near the end of the log phase....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.1.74-80.1968

    authors: Stockland AE,San Clemente CL

    更新日期:1968-01-01 00:00:00

  • Xenorhabdus nematophilus as a model for host-bacterium interactions: rpoS is necessary for mutualism with nematodes.

    abstract::Xenorhabdus nematophilus, a gram-negative bacterium, is a mutualist of Steinernema carpocapsae nematodes and a pathogen of larval-stage insects. We use this organism as a model of host-microbe interactions to identify the functions bacteria require for mutualism, pathogenesis, or both. In many gram-negative bacteria, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.16.4687-4693.2001

    authors: Vivas EI,Goodrich-Blair H

    更新日期:2001-08-01 00:00:00

  • Genome sequence of the bacterium Streptomyces davawensis JCM 4913 and heterologous production of the unique antibiotic roseoflavin.

    abstract::Streptomyces davawensis JCM 4913 synthesizes the antibiotic roseoflavin, a structural riboflavin (vitamin B(2)) analog. Here, we report the 9,466,619-bp linear chromosome of S. davawensis JCM 4913 and a 89,331-bp linear plasmid. The sequence has an average G+C content of 70.58% and contains six rRNA operons (16S-23S-5...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01592-12

    authors: Jankowitsch F,Schwarz J,Rückert C,Gust B,Szczepanowski R,Blom J,Pelzer S,Kalinowski J,Mack M

    更新日期:2012-12-01 00:00:00

  • Staphylococcal bacteriophage-associated lysin: a lytic agent active against Staphylococcus aureus.

    abstract::A lytic enzyme active against viable, intact staphylococci is released into culture fluids upon lysis of bacteriophage-infected Staphylococcus aureus PS53 cells. This enzyme, staphylococcal phage-associated lysin (PAL), was partially purified by ammonium sulfate precipitation and gel filtration through Sephadex G-200....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.107.2.499-504.1971

    authors: Sonstein SA,Hammel JM,Bondi A

    更新日期:1971-08-01 00:00:00

  • Molecules involved in the modulation of rapid cell death in Xanthomonas.

    abstract::In earlier studies from this laboratory, Xanthomonas campestris pv. glycines was found to exhibit a nutrition stress-related postexponential rapid cell death (RCD). The RCD was exhibited in protein-rich media but not in starch or other minimal media. This RCD in X. campestris pv. glycines was found to display features...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00056-06

    authors: Raju KK,Gautam S,Sharma A

    更新日期:2006-08-01 00:00:00

  • Transcriptional regulation of the heat shock regulatory gene rpoH in Escherichia coli: involvement of a novel catabolite-sensitive promoter.

    abstract::A catabolite-sensitive promoter was found to be involved in transcription of the heat shock regulatory gene rpoH encoding the sigma 32 protein. Expression of lacZ from the operon fusion, rpoHp-lacZ, was partially inhibited by glucose added to the broth medium. Dissection of the rpoH promoter region allowed us to local...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.5.2710-2715.1990

    authors: Nagai H,Yano R,Erickson JW,Yura T

    更新日期:1990-05-01 00:00:00

  • Reproduction and morphogenesis of Rickettsiella chironomi, an unusual intracellular procaryotic parasite of midge larvae.

    abstract::The reproduction and morphogenesis of Rickettsiella chironomi, an unusual procaryotic parasite of midge larvae, was studied with electron microscopy. The morphogenic cycle was similar to that found in chlamydia and consisted of four major cell types: (i) medium-sized spherical initial bodies (ca. 1 micrometers in diam...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.143.2.995-1002.1980

    authors: Federici BA

    更新日期:1980-08-01 00:00:00

  • Regulation of the Salmonella typhimurium aroF gene in Escherichia coli.

    abstract::The Salmonella typhimurium aroF gene, encoding the tyrosine-sensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase, was localized to a chromosomal PstI fragment by Southern blotting with an Escherichia coli aroF probe. This fragment was cloned by screening a plasmid library for complementation of an E. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.5.2259-2266.1990

    authors: Muday GK,Herrmann KM

    更新日期:1990-05-01 00:00:00

  • cobB function is required for catabolism of propionate in Salmonella typhimurium LT2: evidence for existence of a substitute function for CobB within the 1,2-propanediol utilization (pdu) operon.

    abstract::The cobB function of Salmonella typhimurium LT2 was defined in vivo as an alternative activity for the nicotinic acid mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase enzyme (CobT), which is involved in the assembly of the nucleotide loop of cobalamin in this bacterium (J. R. Trzebiatowski, G. A. O'T...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.23.7016-7019.1996

    authors: Tsang AW,Escalante-Semerena JC

    更新日期:1996-12-01 00:00:00

  • Nucleotide sequence of a Pseudomonas denitrificans 5.4-kilobase DNA fragment containing five cob genes and identification of structural genes encoding S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase and cobyrinic acid a,c-diamide synthase.

    abstract::A 5.4-kilobase DNA fragment carrying Pseudomonas denitrificans cob genes has been sequenced. The nucleotide sequence and genetic analysis revealed that this fragment carries five different cob genes (cobA to cobE). Four of these genes present the characteristics of translationally coupled genes. cobA has been identifi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.10.5968-5979.1990

    authors: Crouzet J,Cauchois L,Blanche F,Debussche L,Thibaut D,Rouyez MC,Rigault S,Mayaux JF,Cameron B

    更新日期:1990-10-01 00:00:00

  • Anaerobic control of denitrification in Pseudomonas stutzeri escapes mutagenesis of an fnr-like gene.

    abstract::The synthesis of proteins necessary for the respiratory reduction of nitrate to dinitrogen is induced in most denitrifying bacteria by a shift to anaerobiosis. A homolog of the fur gene, which encodes a redox-active transcriptional activator in Escherichia coli, was isolated from Pseudomonas stutzeri by using the anr ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.22.7236-7246.1993

    authors: Cuypers H,Zumft WG

    更新日期:1993-11-01 00:00:00

  • Identification of Treponema pallidum penicillin-binding proteins.

    abstract::Penicillin-binding proteins of 180, 89, 80, 68, 61, 41, and 38 kilodaltons were identified in Treponema pallidum (Nichols) by their covalent binding of [35S]benzylpenicillin. Penicillin-binding proteins are localized in the plasma membranes of many bacterial species and may serve as useful markers for determining plas...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.11.5298-5300.1987

    authors: Cunningham TM,Miller JN,Lovett MA

    更新日期:1987-11-01 00:00:00

  • Effect of D-amino acids on structure and synthesis of peptidoglycan in Escherichia coli.

    abstract::Growth of Escherichia coli in the presence of certain D-amino acids, such as D-methionine, results in the incorporation of the D-amino acid into macromolecular peptidoglycan and can be lethal at high concentrations. Previous studies suggested that incorporation was independent of the normal biosynthetic pathway. An en...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.17.5549-5559.1992

    authors: Caparrós M,Pisabarro AG,de Pedro MA

    更新日期:1992-09-01 00:00:00

  • Transcription of the Escherichia coli fumarate reductase genes (frdABCD) and their coordinate regulation by oxygen, nitrate, and fumarate.

    abstract::The fumarate reductase enzyme complex allows Escherichia coli to grow anaerobically with fumarate as a terminal electron acceptor for oxidative phosphorylation when the preferred compounds oxygen and nitrate are not available. We used the pKO promoter test vectors to identify a single promoter for the frdABCD genes wh...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.3.1100-1109.1985

    authors: Jones HM,Gunsalus RP

    更新日期:1985-12-01 00:00:00

  • ANTIMICROBIAL ACTION OF NITROGEN-CONTAINING STEROIDS.

    abstract::Smith, Rodney F. (University of Maryland, Baltimore), Donald E. Shay, and Norman J. Doorenbos. Antimicrobial action of nitrogen-containing steroids. J. Bacteriol. 85:1295-1299. 1963.-A new group of 16 synthetic nitrogen-containing steroids have been tested against a variety of microorganisms for antimicrobial properti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.85.6.1295-1299.1963

    authors: SMITH RF,SHAY DE,DOORENBOS NJ

    更新日期:1963-06-01 00:00:00

  • A region in the Bacillus subtilis transcription factor Spo0A that is important for spoIIG promoter activation.

    abstract::Spo0A is a DNA binding protein in Bacillus subtilis required for the activation of spoIIG and other promoters at the onset of endospore formation. Activation of some of these promoters may involve interaction of Spo0A and the sigmaA subunit of RNA polymerase. Previous studies identified two single-amino-acid substitut...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.14.3578-3583.1998

    authors: Buckner CM,Schyns G,Moran CP Jr

    更新日期:1998-07-01 00:00:00

  • Functional Overlap of hetP and hetZ in Regulation of Heterocyst Differentiation in Anabaena sp. Strain PCC 7120.

    abstract::HetR plays a key role in regulation of heterocyst differentiation and patterning in Anabaena It directly regulates genes involved in heterocyst differentiation (such as hetP and hetZ), genes involved in pattern formation (patA), and many others. In this study, we investigated the functional relationship of hetP and he...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00707-17

    authors: Zhang H,Wang S,Wang Y,Xu X

    更新日期:2018-04-09 00:00:00

  • sn-Glycerol-3-phosphate transport in Salmonella typhimurium.

    abstract::Salmonella typhimurium contains a transport system for sn-glycerol-3-phosphate that is inducible by growth on glycerol and sn-glycerol-3-phosphate. In fully induced cells, the system exhibited an apparent Km of 50 microM and a Vmax of 2.2 nmol/min . 10(8) cells. The corresponding system in Escherichia coli exhibits, u...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.155.1.186-195.1983

    authors: Hengge R,Larson TJ,Boos W

    更新日期:1983-07-01 00:00:00

  • Adaptation of Mycobacterium smegmatis to stationary phase.

    abstract::Mycobacterium tuberculosis can persist for many years within host lung tissue without causing clinical disease. Little is known about the state in which the bacilli survive, although it is frequently referred to as dormancy. Some evidence suggests that cells survive in nutrient-deprived stationary phase. Therefore, we...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.1.270-283.1999

    authors: Smeulders MJ,Keer J,Speight RA,Williams HD

    更新日期:1999-01-01 00:00:00

  • Characterization of a human serum inhibitor of Clostridium histolyticum proteinase(s).

    abstract::Normal animal sera inhibit at least one Clostridium histolyticum proteinase. An assay procedure based on immune hemolysis was developed for the estimation of this inhibition. This inhibitory activity occurs in various levels in the sera of different animal species. The highest titers have been obtained with rat sera. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.6.1969-1976.1968

    authors: Luzzati A,Goldlust MB,Levine L

    更新日期:1968-12-01 00:00:00

  • Plasmid and transposon transfer to Thiobacillus ferrooxidans.

    abstract::The broad-host-range IncP plasmids RP4, R68.45, RP1::Tn501, and pUB307 were transferred to acidophilic, obligately chemolithotrophic Thiobacillus ferrooxidans from Escherichia coli by conjugation. A genetic marker of kanamycin resistance was expressed in T. ferrooxidans. Plasmid RP4 was transferred back to E. coli fro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.10.2892-2897.1994

    authors: Peng JB,Yan WM,Bao XZ

    更新日期:1994-05-01 00:00:00

  • Gene order of the TOL catabolic plasmid upper pathway operon and oxidation of both toluene and benzyl alcohol by the xylA product.

    abstract::TOL plasmid pWW0 specifies enzymes for the oxidative catabolism of toluene and xylenes. The upper pathway converts the aromatic hydrocarbons to aromatic carboxylic acids via corresponding alcohols and aldehydes and involves three enzymes: xylene oxygenase, benzyl alcohol dehydrogenase, and benzaldehyde dehydrogenase. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.167.2.455-461.1986

    authors: Harayama S,Leppik RA,Rekik M,Mermod N,Lehrbach PR,Reineke W,Timmis KN

    更新日期:1986-08-01 00:00:00

  • tRNA Modification Profiles and Codon-Decoding Strategies in Methanocaldococcus jannaschii.

    abstract::tRNAs play a critical role in mRNA decoding, and posttranscriptional modifications within tRNAs drive decoding efficiency and accuracy. The types and positions of tRNA modifications in model bacteria have been extensively studied, and tRNA modifications in a few eukaryotic organisms have also been characterized and lo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00690-18

    authors: Yu N,Jora M,Solivio B,Thakur P,Acevedo-Rocha CG,Randau L,de Crécy-Lagard V,Addepalli B,Limbach PA

    更新日期:2019-04-09 00:00:00

  • Mapping of a structural gene for valyl-transfer ribonucleic acid synthetase in Escherichia coli by transduction.

    abstract::A structural gene, valS, for the valyl-transfer ribonucleic acid synthetase of Escherichia coli has been mapped on the clockwise side of pyrB and is closely linked to it. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.2.837-839.1969

    authors: Tingle MA,Neidhardt FC

    更新日期:1969-05-01 00:00:00