Abstract:
:The filamentous cyanobacterium Anabaena fixes nitrogen in specialized cells called heterocysts. The immediate product of fixation, ammonia, is known to be assimilated by addition to glutamate to make glutamine. How fixed nitrogen is transported along the filament to the 10 to 20 vegetative cells that separate heterocysts is unknown. N-fixing heterocysts accumulate an insoluble polymer containing aspartate and arginine at the cell poles. Lockau's group has proposed that the polymer is degraded at the poles to provide a mobile carrier, arginine, to the vegetative cells (R. Richter, M. Hejazi, R. Kraft, K. Ziegler, and W. Lockau, Eur. J. Biochem. 263:163-169, 1999). We wished to use the Sakaguchi reaction for arginine to determine the relative cellular concentration of arginine along the filament. At present, the methods for measuring absorption of the Sakaguchi reaction product at 520 nm are insufficiently sensitive for that purpose. However, that product quenches the fluorescence of phycobiliproteins, which we have adapted to a determination of arginine. Our results are consistent with the proposal that arginine is a principal nitrogen carrier from heterocysts to vegetative cells in Anabaena.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Ke S,Haselkorn Rdoi
10.1128/JB.01512-12subject
Has Abstractpub_date
2013-01-01 00:00:00pages
25-8issue
1eissn
0021-9193issn
1098-5530pii
JB.01512-12journal_volume
195pub_type
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journal_title:Journal of bacteriology
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更新日期:1992-11-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.4.785-792.1998
更新日期:1998-02-01 00:00:00
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更新日期:1965-08-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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更新日期:1983-09-01 00:00:00
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更新日期:2001-11-01 00:00:00
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更新日期:1979-01-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1983-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.2.768-770.1982
更新日期:1982-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1982-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1985-01-01 00:00:00