Extracellular exonuclease as a stage 0 biochemical marker in Bacillus subtilis sporulation.

abstract：:Six analogues of amino acids killed corresponding auxotrophs of Escherichia coli. With all but one analogue, protein synthesis was required for lethality. ...

journal_title：Journal of bacteriology

authors： Rabinovitz M,Finkleman A,Reagan RL,Breitman TR

更新日期：1969-07-01 00:00:00

abstract：:An increase in the apparent molecular weight of the principal outer membrane protein (POMP) of Neisseria gonorrhoeae is associated with introduction of the penB2 genetic marker, which results in low-level, relatively nonspecific antibiotic resistance. Limited proteolysis of the two forms of POMP showed that they had f...

journal_title：Journal of bacteriology

authors： Cannon JG,Klapper DG,Blackman EY,Sparling PF

更新日期：1980-08-01 00:00:00

abstract：:The rates of thermal denaturation and the molecular weights of the two isofunctional enol-lactone hydrolases (ELH I and ELH II) of Acinetobacter calcoaceticus were determined. The molecular weights of ELH I and ELH II were found by gel filtration to be approximately 24,000 and 21,000, respectively. In crude extract at...

journal_title：Journal of bacteriology

authors： Katagiri M,Wheelis ML

更新日期：1971-05-01 00:00:00

abstract：:The expression of the cyanobacterial recA gene, isolated from Anabaena variabilis, has been examined at the levels of transcript and protein abundance. Exposure of the cyanobacterium to a variety of DNA-damaging agents, including mitomycin C, methyl methanesulfonate, and UV irradiation, results in a rapid increase in ...

journal_title：Journal of bacteriology

authors： Owttrim GW,Coleman JR

更新日期：1989-10-01 00:00:00

abstract：:Mutants of Thiosphaera pantotropha defective in chemolithoautotrophic growth were obtained by transpositional mutagenesis with Tn5 coding for kanamycin resistance. The suicide vehicle for introducing Tn5 to T. pantotropha was pSUP5011 harbored by Escherichia coli. Kanamycin-resistant isolates were screened for the ina...

journal_title：Journal of bacteriology

authors： Chandra TS,Friedrich CG

更新日期：1986-05-01 00:00:00

abstract：:The transforming activity of fractionated complementary strands of Diplococcus pneumoniae deoxyribonucleic acid (DNA) bands at the position of fully denatured DNA in CsCl at pH 11.0, and is completely (> 99.8%) destroyed by digestion with exonuclease-I. These results prove that pure single strands transform the normal...

journal_title：Journal of bacteriology

authors： Miao R,Guild WR

更新日期：1970-02-01 00:00:00

abstract：:A cluster of Bacillus subtilis fatty acid synthetic genes was isolated by complementation of an Escherichia coli fabD mutant encoding a thermosensitive malonyl coenzyme A-acyl carrier protein transacylase. The B. subtilis genomic segment contains genes that encode three fatty acid synthetic proteins, malonyl coenzyme ...

journal_title：Journal of bacteriology

authors： Morbidoni HR,de Mendoza D,Cronan JE Jr

更新日期：1996-08-01 00:00:00

abstract：:Glucosylated deoxyribonucleic acid (DNA) from phages T4 and T6 competes poorly with homologous DNA causing only a slight decrease of transformation in Group H Streptococcus strain Challis. Other types of heterologous DNAs (Micrococcus luteus, Clostridium perfringens, Escherichia coli, calf thymus and non-glucosylated ...

journal_title：Journal of bacteriology

authors： Ceglowski P,Fuchs PG,Soltyk A

更新日期：1975-12-01 00:00:00

abstract：:The well-known large catabolic potential of rhodococci is greatly facilitated by an impressive gene multiplicity. This study reports on the multiplicity of kshA, encoding the oxygenase component of 3-ketosteroid 9α-hydroxylase, a key enzyme in steroid catabolism. Five kshA homologues (kshA1 to kshA5) were previously i...

journal_title：Journal of bacteriology

authors： Petrusma M,Hessels G,Dijkhuizen L,van der Geize R

更新日期：2011-08-01 00:00:00

abstract：:Strain 768-28 was isolated from a hot spring in Kamchatka, Russia, and represents a novel member of the Vulcanisaeta genus. The complete genome sequence of this thermoacidophilic anaerobic crenarchaeon reveals genes for protein and carbohydrate-active enzymes, the Embden-Meyerhof and Entner-Doudoroff pathways for gluc...

journal_title：Journal of bacteriology

authors： Gumerov VM,Mardanov AV,Beletsky AV,Prokofeva MI,Bonch-Osmolovskaya EA,Ravin NV,Skryabin KG

更新日期：2011-05-01 00:00:00

abstract：:The genetic requirements for the excision repair of thymine glycols, urea residues, and apurinic (AP) sites were examined by measuring the survival in Escherichia coli mutants of phi X174 replicative form (RF) I transfecting DNA containing selectively introduced lesions. phi X RF I DNA containing thymine glycols was i...

journal_title：Journal of bacteriology

authors： Laspia MF,Wallace SS

更新日期：1988-08-01 00:00:00

abstract：:The major conjugative transfer gene cluster of staphylococcal plasmid pGO1 (trs) consists of 13 open reading frames (trsA to trsM) transcribed from one DNA strand and a single 189-bp open reading frame (trsN) within the first 348 bp of trs that is transcribed divergently. Promoter regions for trsN and trsA partially o...

journal_title：Journal of bacteriology

authors： Sharma VK,Johnston JL,Morton TM,Archer GL

更新日期：1994-06-01 00:00:00

abstract：:The folded chromosome of Escherichia coli has been investigated under various lysis and physiological conditions. A new gradient system was devised that allows excellent separation between unlysed cells and envelope-associated and envelope-free chromosomes. Isotope incorporation experiments showed that the fraction of...

journal_title：Journal of bacteriology

authors： Korch C,Ovrebo S,Kleppe K

更新日期：1976-08-01 00:00:00

abstract：:A modified 3-hydroxypropionate cycle has been proposed as the autotrophic CO2 fixation pathway for the thermoacidophilic crenarchaeon Metallosphaera sedula. The cycle requires the reductive conversion of 3-hydroxypropionate to propionyl-coenzyme A (propionyl-CoA). The specific activity of the 3-hydroxypropionate-, CoA...

journal_title：Journal of bacteriology

authors： Alber BE,Kung JW,Fuchs G

更新日期：2008-02-01 00:00:00

abstract：:The chemical composition of the cell walls of several L-form revertants derived from Nocardia asteroides 10905 was determined at different stages of growth. It was observed that each L-form revertant had a cell well that differed from that of the parental strain when grown under identical conditions. In some strains t...

journal_title：Journal of bacteriology

authors： Beaman BL,Bourgeois AL,Moring SE

更新日期：1981-11-01 00:00:00

abstract：:The extracellular protease of Pseudomonas maltophilia was partially purified by ammonium sulfate precipitation and chromatography on Sephadex G-75 and Bio-rex 70. Gel electrophoresis revealed minor impurities. The enzyme exhibited the following properties: (i) molecular weight, 35,000; (ii) A see article; 10.8; (iii) ...

journal_title：Journal of bacteriology

authors： Boethling RS

更新日期：1975-03-01 00:00:00

abstract：:We have characterized an acyl carrier protein (ACP) presumed to be involved in the synthesis of fatty acids in Streptomyces coelicolor A3(2). This is the third ACP to have been identified in S. coelicolor; the two previously characterized ACPs are involved in the synthesis of two aromatic polyketides: the blue-pigment...

journal_title：Journal of bacteriology

authors： Revill WP,Bibb MJ,Hopwood DA

更新日期：1996-10-01 00:00:00

abstract：:The nucleotide sequence of a 7.3-kilobase-pair fragment of DNA encoding a hemolytic activity from Serratia marcescens was determined. Two large open reading frames were identified, designated shlA (Serratia hemolysin) and shlB, capable of encoding polypeptides of 165, 056 and 61,897 molecular weight, respectively. Bot...

journal_title：Journal of bacteriology

authors： Poole K,Schiebel E,Braun V

更新日期：1988-07-01 00:00:00

abstract：:Huston, Charles K. (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.), and Phillip W. Albro. Lipids of Sarcina lutea. I. Fatty acid composition of the extractable lipids. J. Bacteriol. 88:425-432. 1964.-The extractable lipids of Sarcina lutea were separated into several fractions by a combination of col...

journal_title：Journal of bacteriology

authors： HUSTON CK,ALBRO PW

更新日期：1964-08-01 00:00:00

abstract：:We present the complete genome sequence of Yersinia pestis KIM, the etiologic agent of bubonic and pneumonic plague. The strain KIM, biovar Mediaevalis, is associated with the second pandemic, including the Black Death. The 4.6-Mb genome encodes 4,198 open reading frames (ORFs). The origin, terminus, and most genes en...

journal_title：Journal of bacteriology

authors： Deng W,Burland V,Plunkett G 3rd,Boutin A,Mayhew GF,Liss P,Perna NT,Rose DJ,Mau B,Zhou S,Schwartz DC,Fetherston JD,Lindler LE,Brubaker RR,Plano GV,Straley SC,McDonough KA,Nilles ML,Matson JS,Blattner FR,Perry RD

更新日期：2002-08-01 00:00:00

abstract：:Integrative and conjugative elements (ICEs), also called conjugative transposons, are genomic islands that excise, self-transfer by conjugation, and integrate in the genome of the recipient bacterium. The current investigation shows the intraspecies conjugative transfer of the first described ICEs in Streptococcus the...

journal_title：Journal of bacteriology

authors： Bellanger X,Roberts AP,Morel C,Choulet F,Pavlovic G,Mullany P,Decaris B,Guédon G

更新日期：2009-04-01 00:00:00

abstract：:We have cloned a lipopolysaccharide (LPS) biosynthetic gene from Pseudomonas aeruginosa PAO1 that complements the defect in the production and incorporation of LPS O side chains in the LPS-rough strain AK1012. This gene was characterized by pulsed-field gel electrophoresis, deletion and restriction mapping of the clon...

journal_title：Journal of bacteriology

authors： Goldberg JB,Hatano K,Pier GB

更新日期：1993-03-01 00:00:00

abstract：:PsaA, the subunit of the fimbria originally referred to as the "pH 6 antigen," is required for full virulence of Yersinia pestis during bubonic plague. The expression of psaA is dependent upon specific environmental signals, and while the signals (high temperature and acidic pH) are defined, the mechanisms underlying ...

journal_title：Journal of bacteriology

authors： Quinn JD,Weening EH,Miner TA,Miller VL

更新日期：2019-07-24 00:00:00

abstract：:Pep 5 and nisin are cationic peptide antibiotics which in addition to their membrane-disruptive action induce autolysis in staphylococci. To investigate the mechanism of lysis induction, the influence of the peptides on the activity of the N-acetylmuramoyl-L-alanine amidase of Staphylococcus simulans 22 was studied. I...

journal_title：Journal of bacteriology

authors： Bierbaum G,Sahl HG

更新日期：1987-12-01 00:00:00

abstract：:The ToxR protein of Vibrio cholerae is an integral membrane protein that coordinately regulates the expression of virulence genes required for successful infection. ToxR has been shown to bind directly to and activate transcription of the cholera toxin (ctx) promoter. Within the amino-terminal cytoplasmic region of To...

journal_title：Journal of bacteriology

authors： Ottemann KM,DiRita VJ,Mekalanos JJ

更新日期：1992-11-01 00:00:00

abstract：:A cyclic version of the Entner-Doudoroff pathway is used by Pseudomonas aeruginosa to metabolize carbohydrates. Genes encoding the enzymes that catabolize intracellular glucose to pyruvate and glyceraldehyde 3-phosphate are coordinately regulated, clustered at 39 min on the chromosome, and collectively form the hex re...

journal_title：Journal of bacteriology

authors： Hager PW,Calfee MW,Phibbs PV

更新日期：2000-07-01 00:00:00

abstract：:In acetate-limited chemostat cultures started with single-colony cultures of Thiobacillus versutus, a mutant appeared after approximately 85 volume changes. The inhomogeneity of the culture was detected by the development of two different types of colonies on agar plates. When a pure culture of the mutant was grown in...

journal_title：Journal of bacteriology

authors： Claassen PA,Kortstee GJ,Oosterveld-van Vliet WM,van Neerven AR

更新日期：1986-11-01 00:00:00

abstract：:The recA gene from Thermus thermophilus HB27 was cloned and engineered to obtain insertion (recA::kat) and deletion (deltarecA) derivatives. Transcription of recA in this extreme thermophile was induced by mitomycin C, leading to the synthesis of a monocistronic mRNA. This DNA damage-mediated induction was dependent o...

journal_title：Journal of bacteriology

authors： Castán P,Casares L,Barbé J,Berenguer J

更新日期：2003-08-01 00:00:00

abstract：:Plasmid pP51 of Pseudomonas sp. strain P51 contains two gene clusters encoding the degradation of chlorinated benzenes, tcbAB and tcbCDEF. A regulatory gene, tcbR, was located upstream and divergently transcribed from the chlorocatechol oxidative gene cluster tcbCDEF. The tcbR gene was characterized by DNA sequencing ...

journal_title：Journal of bacteriology

authors： van der Meer JR,Frijters AC,Leveau JH,Eggen RI,Zehnder AJ,de Vos WM

更新日期：1991-06-01 00:00:00

abstract：:The morphology, on MacConkey's agar, of colonies of the bacteria commonly found in enteric specimens is sufficiently characteristic and distinctive, when observed with transmitted oblique illumination and a low-power stereoscopic microscope, to enable identification at the generic (and occasionally the species) level ...

journal_title：Journal of bacteriology

authors： Finkelstein RA,Punyashthiti K

更新日期：1967-06-01 00:00:00