Bacillus subtilis acyl carrier protein is encoded in a cluster of lipid biosynthesis genes.

Abstract:

:A cluster of Bacillus subtilis fatty acid synthetic genes was isolated by complementation of an Escherichia coli fabD mutant encoding a thermosensitive malonyl coenzyme A-acyl carrier protein transacylase. The B. subtilis genomic segment contains genes that encode three fatty acid synthetic proteins, malonyl coenzyme A-acyl carrier protein transacylase (fabD), 3-ketoacyl-acyl carrier protein reductase (fabG), and the N-terminal 14 amino acid residues of acyl carrier protein (acpP). Also present is a sequence that encodes a homolog of E. coli plsX, a gene that plays a poorly understood role in phospholipid synthesis. The B. subtilis plsX gene weakly complemented an E. coli plsX mutant. The order of genes in the cluster is plsX fabD fabG acpP, the same order found in E. coli, except that in E. coli the fabH gene lies between plsX and fabD. The absence of fabH in the B. subtilis cluster is consistent with the different fatty acid compositions of the two organisms. The amino acid sequence of B. subtilis acyl carrier protein was obtained by sequencing the purified protein, and the sequence obtained strongly resembled that of E. coli acyl carrier protein, except that most of the protein retained the initiating methionine residue. The B. subtilis fab cluster was mapped to the 135 to 145 degrees region of the chromosome.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Morbidoni HR,de Mendoza D,Cronan JE Jr

doi

10.1128/jb.178.16.4794-4800.1996

subject

Has Abstract

pub_date

1996-08-01 00:00:00

pages

4794-800

issue

16

eissn

0021-9193

issn

1098-5530

journal_volume

178

pub_type

杂志文章
  • Cellular autolytic activity in synchronized populations of Streptococcus faecium.

    abstract::The autolytic capacity of Streptococcus faecium (S. faecalis ATCC 9790) varied during synchronous cell division. This phenomenon was initially observed in rapidly dividing populations (TD=30 to 33 min) synchronized by a combination of induction and size selection techniques. To minimize the problems inherent in studie...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.133.2.822-829.1978

    authors: Hinks RP,Daneo-Moore L,Shockman GD

    更新日期:1978-02-01 00:00:00

  • Sialidase-like enzymes produced by group A, B, C, G, and L streptococci and by Streptococcus sanguis.

    abstract::A group of enzymes were prepared from the culture fluids of streptococci belonging to groups A, B, C, G, and L, and from a strain of Streptococcus sanguis. These streptococcal enzymes (designated St-sialidases) released a substance shown to belong to the sialic acid group from the specific substrate BSM-St, a sialomuc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.97.3.1328-1333.1969

    authors: Hayano S,Tanaka A

    更新日期:1969-03-01 00:00:00

  • Comparative genome sequence analysis of multidrug-resistant Acinetobacter baumannii.

    abstract::The recent emergence of multidrug resistance (MDR) in Acinetobacter baumannii has raised concern in health care settings worldwide. In order to understand the repertoire of resistance determinants and their organization and origins, we compared the genome sequences of three MDR and three drug-susceptible A. baumannii ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00834-08

    authors: Adams MD,Goglin K,Molyneaux N,Hujer KM,Lavender H,Jamison JJ,MacDonald IJ,Martin KM,Russo T,Campagnari AA,Hujer AM,Bonomo RA,Gill SR

    更新日期:2008-12-01 00:00:00

  • Purification and characterization of two extremely thermostable enzymes, phosphate acetyltransferase and acetate kinase, from the hyperthermophilic eubacterium Thermotoga maritima.

    abstract::Phosphate acetyltransferase (PTA) and acetate kinase (AK) of the hyperthermophilic eubacterium Thermotoga maritima have been purified 1,500- and 250-fold, respectively, to apparent homogeneity. PTA had an apparent molecular mass of 170 kDa and was composed of one subunit with a molecular mass of 34 kDa, suggesting a h...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.6.1861-1867.1999

    authors: Bock AK,Glasemacher J,Schmidt R,Schönheit P

    更新日期:1999-03-01 00:00:00

  • Cloning, characterization, and effects of overexpression of the Escherichia coli rnd gene encoding RNase D.

    abstract::RNase D is a 3'-exoribonuclease whose in vitro specificity has suggested that it is involved in the processing of tRNA precursors. Its in vivo role has remained unclear, however, because mutant cells devoid of the enzyme display no defect in growth or tRNA processing. To learn more about the structure and function of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.2.522-527.1988

    authors: Zhang JR,Deutscher MP

    更新日期:1988-02-01 00:00:00

  • Involvement of outer membrane protein TolC, a possible member of the mar-sox regulon, in maintenance and improvement of organic solvent tolerance of Escherichia coli K-12.

    abstract::Escherichia coli mutants with improved organic solvent tolerance levels showed high levels of outer membrane protein TolC and inner membrane protein AcrA. The TolC level was regulated positively by MarA, Rob, or SoxS. A possible mar-rob-sox box sequence was found upstream of the tolC gene. These findings suggest that ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.4.938-944.1998

    authors: Aono R,Tsukagoshi N,Yamamoto M

    更新日期:1998-02-01 00:00:00

  • Kinetic analysis of the products of donor deoxyribonucleate in transformed cells of Bacillus subtilis.

    abstract::This paper describes the major transmutations of donor deoxyribonucleic acid (DNA) after uptake by competent Bacillus subtilis cells. Kinetic experiments confirm that after exposure to competent cells, donor DNA is converted to double-stranded fragments (DSF) which can be isolated as early as 30 s from the beginning o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.116.1.154-162.1973

    authors: Davidoff-Abelson R,Dubnau D

    更新日期:1973-10-01 00:00:00

  • Positive selection for loss-of-function tat mutations identifies critical residues required for TatA activity.

    abstract::The Tat system, found in the cytoplasmic membrane of many bacteria, is a general export pathway for folded proteins. Here we describe the development of a method, based on the transport of chloramphenicol acetyltransferase, that allows positive selection of mutants defective in Tat function. We have demonstrated the u...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.8.2920-2925.2005

    authors: Hicks MG,Lee PA,Georgiou G,Berks BC,Palmer T

    更新日期:2005-04-01 00:00:00

  • Cloning, sequence analysis, and characterization of the genes involved in isoprimeverose metabolism in Lactobacillus pentosus.

    abstract::Two genes, xylP and xylQ, from the xylose regulon of Lactobacillus pentosus were cloned and sequenced. Together with the repressor gene of the regulon, xylR, the xylPQ genes form an operon which is inducible by xylose and which is transcribed from a promoter located 145 bp upstream of xylP. A putative xylR binding sit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.9.2312-2320.1998

    authors: Chaillou S,Lokman BC,Leer RJ,Posthuma C,Postma PW,Pouwels PH

    更新日期:1998-05-01 00:00:00

  • Buoyant density, growth rate, and the cell cycle in Streptococcus faecium.

    abstract::The buoyant density in rapidly growing Streptococcus faecium 9790 cells varies over the cell cycle, in contrast to the density in Escherichia coli. Buoyant density in S. faecium was measured by using Percoll (Pharmacia Fine Chemicals, Piscataway, N.J.) density gradients. We found that the mean and coefficient of varia...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.2.669-673.1989

    authors: Glaser D,Higgins M

    更新日期:1989-02-01 00:00:00

  • Chimeric streptococcal plasmids and their use as molecular cloning vehicles in Streptococcus sanguis (Challis).

    abstract::Chimeric plasmids, which were useful as cloning vehicles in a Streptococcus sanguis (Challis) host vector system, have been constructed. By using three different strategies of restriction endonuclease digestion and ligation, a deoxyribonucleic acid (DNA) fragment bearing an erythromycin resistance determinant was liga...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.143.3.1425-1435.1980

    authors: Macrina FL,Jones KR,Wood PH

    更新日期:1980-09-01 00:00:00

  • Quorum sensing negatively controls type III secretion regulon expression in Pseudomonas aeruginosa PAO1.

    abstract::A systematic analysis of the type III secretion (T3S) genes of Pseudomonas aeruginosa strain PAO1 revealed that they are under quorum-sensing control. This observation was supported by the down-regulation of the T3S regulon in the presence of RhlR-C4HSL and the corresponding advanced secretion of ExoS in a rhlI mutant...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.11.3898-3902.2005

    authors: Bleves S,Soscia C,Nogueira-Orlandi P,Lazdunski A,Filloux A

    更新日期:2005-06-01 00:00:00

  • Biochemical and serological evidence for an RNase E-like activity in halophilic Archaea.

    abstract::Endoribonuclease RNase E appears to control the rate-limiting step that mediates the degradation of many mRNA species in bacteria. In this work, an RNase E-like activity in Archaea is described. An endoribonucleolytic activity from the extreme halophile Haloarcula marismortui showed the same RNA substrate specificity ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.4.1180-1185.1997

    authors: Franzetti B,Sohlberg B,Zaccai G,von Gabain A

    更新日期:1997-02-01 00:00:00

  • High-level production of -galactosidase by Escherichia coli merodiploids.

    abstract::Two merodiploids of Escherichia coli that contain genes for the lac operon on both chromosome and episome were tested for production of lac enzymes after growth on various carbon sources. The specific activity of beta-galactosidase (and of thiogalactoside transacetylase) was about twice that from haploid cells when gr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.2.856-860.1972

    authors: Fowler AV

    更新日期:1972-11-01 00:00:00

  • Characterization of xylanolytic enzymes in Clostridium cellulovorans: expression of xylanase activity dependent on growth substrates.

    abstract::Xylanase activity of Clostridium cellulovorans, an anaerobic, mesophilic, cellulolytic bacterium, was characterized. Most of the activity was secreted into the growth medium when the bacterium was grown on xylan. Furthermore, when the extracellular material was separated into cellulosomal and noncellulosomal fractions...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.24.7037-7043.2001

    authors: Kosugi A,Murashima K,Doi RH

    更新日期:2001-12-01 00:00:00

  • Mechanism of tuberculostasis in mammalian serum. II. Induction of serum tuberculostasis in guinea pigs.

    abstract::The growth of tubercle bacilli in serum samples of untreated animals depends upon the availability of ionic iron which serves as a growth factor in supporting bacillary multiplication. The amount of available iron in serum is determined by the ratio between iron-saturated and iron-free transferrin; a low value for the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.1.64-70.1969

    authors: Kochan I,Golden CA,Bukovic JA

    更新日期:1969-10-01 00:00:00

  • Purification and properties of a bacteriophage-induced cell wall peptidase from Staphylococcus aureus.

    abstract::A phage-induced cell wall solubilizing enzyme isolated from phage-infected Staphylococcus aureus phage type 80 was purified 588-fold. The pH optimal activity was 6.8 to 7.3, and pH optimal stability, 6.5 to 7.5. It was inhibited by p-hydroxymercuribenzoate, ethylenediaminetetraacetic acid, and specific rabbit antisera...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.3.1089-1095.1967

    authors: Doughty CC,Mann JA

    更新日期:1967-03-01 00:00:00

  • Formation of protoporphyrin from coproporphyrinogen in extracts of various bacteria.

    abstract::The enzyme system capable of converting coproporphyrinogen to protoporphyrin was demonstrated in the soluble fraction of extracts of Pseudomonas fluorescens grown aerobically, of P. denitrificans grown anaerobically under denitrifying conditions, and of Escherichia coli grown both aerobically and anaerobically. Protop...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.102.2.398-403.1970

    authors: Jacobs NJ,Jacobs JM,Brent P

    更新日期:1970-05-01 00:00:00

  • Effect of temperature on the distribution of membrane particles in Streptococcus faecalis as seen by the freeze-fracture technique.

    abstract::When cells of Streptococcus faecalis ATCC 9790 were incubated at temperatures above 10 C before being frozen for freeze-fracture, a random distribution of particles was observed on the outer fracture face of the freeze-cleaved cell membrane. However, when cells were incubated below 10 C before freezing, particleless p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.118.2.725-734.1974

    authors: Tsien HC,Higgins ML

    更新日期:1974-05-01 00:00:00

  • Meeting report on the ASM Conference on Mechanisms of Interbacterial Cooperation and Competition.

    abstract::The ASM Conference on Mechanisms of Interbacterial Cooperation and Competition was held in Washington DC, from 1 to 4 March 2017. The conference provided an international forum for sociomicrobiologists from different disciplines to present and discuss new findings. The meeting covered a wide range of topics, spanning ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00403-17

    authors: Lories B,Parijs I,Foster KR,Steenackers HP

    更新日期:2017-08-14 00:00:00

  • EFFECT OF TEMPERATURE ON THE RATE OF GERMINATION IN BACILLUS CEREUS.

    abstract::Knaysi, Georges (Cornell University, Ithaca, N.Y.). Effect of temperature on the rate of germination in Bacillus cereus. J. Bacteriol. 87:619-622. 1964.-By use of the change of the spores from bright to dark, when observed in dark contrast with a phase microscope, as a criterion for the incipience (first stage) of ger...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.3.619-622.1964

    authors: KNAYSI G

    更新日期:1964-03-01 00:00:00

  • Inhibition of arginyl-transfer ribonucleic acid synthetase activity of Escherichia coli by arginine biosynthetic precursors.

    abstract::The arginine biosynthetic precursors, ornithine, citrulline, and argininosuccinate, inhibit arginyl-transfer ribonucleic acid (tRNA) synthetase (EC 6.1.1.13, arginine: soluble RNA ligase, adenosine monophosphate) activity in the in vitro attachment assay system. Ornithine is the most potent, argininosuccinate is next,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.107.2.589-591.1971

    authors: Yem DW,Williams LS

    更新日期:1971-08-01 00:00:00

  • Occurrence of glutathione in bacteria.

    abstract::Glutathione and soluble thiol content were examined in a broad spectrum of bacteria. Significant soluble thiol was present in all cases. The thiol compound was glutathione in most of the gram-negative bacteria but not in most of the gram-positive bacteria studied. Glutathione was absent in four anerobes and one microa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.133.3.1126-1129.1978

    authors: Fahey RC,Brown WC,Adams WB,Worsham MB

    更新日期:1978-03-01 00:00:00

  • Flavin-linked dehydrogenation of ether glycols by cell-free extracts of a soil bacterium.

    abstract::Payne, W. J. (University of Georgia, Athens), and R. L. Todd. Flavin-linked dehydrogenation of ether glycols by cell-free extracts of a soil bacterium. J. Bacteriol. 91:1533-1536. 1966.-Cell-free extracts of bacterium TEG-5 grown on tetraethylene glycol dehydrogenated a variety of ether glycols and nonylphenoxy and se...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.4.1533-1536.1966

    authors: Payne WJ,Todd RL

    更新日期:1966-04-01 00:00:00

  • Fur regulates expression of the Salmonella pathogenicity island 1 type III secretion system through HilD.

    abstract::The invasion of intestinal epithelial cells by Salmonella enterica serovar Typhimurium is mediated by a type III secretion system (T3SS) encoded on Salmonella pathogenicity island 1 (SPI1). Expression of the SPI1 T3SS is tightly regulated by the combined action of HilC, HilD, and RtsA, three AraC family members that c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00926-07

    authors: Ellermeier JR,Slauch JM

    更新日期:2008-01-01 00:00:00

  • Regulatory role of recF in the SOS response of Escherichia coli: impaired induction of SOS genes by UV irradiation and nalidixic acid in a recF mutant.

    abstract::We isolated a new recF mutant of Escherichia coli K-12 by insertion of transposon Tn5 into the recF gene. This recF400::Tn5 allele displayed the same phenotypic characteristics as the classic recF143 mutation. By using Mu d(Ap lac) fusions, the induction of nine SOS genes, including recA, umuC, dinA, dinB, dinD, dinF,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.4.1731-1736.1987

    authors: Thoms B,Wackernagel W

    更新日期:1987-04-01 00:00:00

  • A long T. A tract in the upp initially transcribed region is required for regulation of upp expression by UTP-dependent reiterative transcription in Escherichia coli.

    abstract::In Escherichia coli, pyrimidine-mediated regulation of upp expression occurs by UTP-sensitive selection of alternative transcriptional start sites, which produces transcripts that differ in the ability to be elongated. The upp initially transcribed region contains the sequence GATTTTTTTTG (nontemplate strand). Initiat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.1.221-228.2001

    authors: Cheng Y,Dylla SM,Turnbough CL Jr

    更新日期:2001-01-01 00:00:00

  • Autoradiographic analysis of diaminopimelic acid incorporation in filamentous cells of Escherichia coli: repression of peptidoglycan synthesis around the nucleoid.

    abstract::Peptidoglycan synthesis rate in nonconstricting filaments of Escherichia coli dnaX(Ts) has been studied by autoradiography of incorporated [3H]diaminopimelic acid. Analysis of autoradiograms of whole cells and sacculi showed that peptidoglycan is synthesized at a reduced rate in the nucleoid-containing parts of these ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.15.4751-4756.1991

    authors: Mulder E,Woldringh CL

    更新日期:1991-08-01 00:00:00

  • pIIICTX, a predicted CTXphi minor coat protein, can expand the host range of coliphage fd to include Vibrio cholerae.

    abstract::CTXphi is a filamentous bacteriophage that encodes cholera toxin. CTXphi infection of its host bacterium, Vibrio cholerae, requires the toxin-coregulated pilus (TCP) and the products of the V. cholerae tolQRA genes. Here, we have explored the role of OrfU, a predicted CTXphi minor coat protein, in CTXphi infection. Pr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.3.1037-1044.2003

    authors: Heilpern AJ,Waldor MK

    更新日期:2003-02-01 00:00:00

  • Genes coding for a new pathway of aerobic benzoate metabolism in Azoarcus evansii.

    abstract::A new pathway for aerobic benzoate oxidation has been postulated for Azoarcus evansii and for a Bacillus stearothermophilus-like strain. Benzoate is first transformed into benzoyl coenzyme A (benzoyl-CoA), which subsequently is oxidized to 3-hydroxyadipyl-CoA and then to 3-ketoadipyl-CoA; all intermediates are CoA thi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.22.6301-6315.2002

    authors: Gescher J,Zaar A,Mohamed M,Schägger H,Fuchs G

    更新日期:2002-11-01 00:00:00