Abstract:
:Payne, W. J. (University of Georgia, Athens), and R. L. Todd. Flavin-linked dehydrogenation of ether glycols by cell-free extracts of a soil bacterium. J. Bacteriol. 91:1533-1536. 1966.-Cell-free extracts of bacterium TEG-5 grown on tetraethylene glycol dehydrogenated a variety of ether glycols and nonylphenoxy and secondary alcohol ethoxy derivatives. Nicotinamide nucleotides did not serve as electron acceptors, but ferricyanide was effective. Dialysis of crude extract depressed activity with tetraethylene glycol, which was restored then by flavine adenine dinucleotide (FAD) or boiled extract supernatant fluid (BES) but not by other flavins. Precipitatation of extract protein at pH 4.0 at 80% ammonium sulfate saturation dissociated FAD and yielded an inactive fraction. Activity was restorable by FAD and BES but not by other flavins. Ethylene glycol was not dehydrogenated by the acid ammonium sulfate fraction with FAD. Atabrine inhibited tetraethylene glycol oxidation, and the inhibition was relieved by FAD but not by other flavins. Tergitols which have sulfated ethoxy side chains on secondary alcohols were not dehydrogenated, but those with free ethoxy side chains on identical alcohols were.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Payne WJ,Todd RLdoi
10.1128/JB.91.4.1533-1536.1966subject
Has Abstractpub_date
1966-04-01 00:00:00pages
1533-6issue
4eissn
0021-9193issn
1098-5530journal_volume
91pub_type
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doi:10.1128/jb.174.7.2193-2198.1992
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更新日期:2017-05-09 00:00:00
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pub_type: 杂志文章
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更新日期:1990-12-01 00:00:00
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更新日期:1970-08-01 00:00:00
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更新日期:1969-12-01 00:00:00
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更新日期:1982-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1967-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1992-04-01 00:00:00