Abstract:
:We determined, under several growth conditions, the kinetics of mRNA synthesis from the four Pseudomonas putida pWW0 plasmid promoters involved in degradation of xylenes and methylbenzyl alcohols via toluates. Transcription by XylS of the meta-cleavage pathway operon promoter (Pm) for the metabolism of alkylbenzoates was stimulated immediately after the addition of an effector, both in Luria-Bertani (LB) medium and in minimal medium. Activation of the sigma 54-dependent upper-pathway operon promoter (Pu) and the xylS gene promoter (Ps) by effector-activated XylR was dependent on the growth medium used: on minimal medium, activation of transcription from Pu and Ps occurred immediately after the addition of a XylR effector; in contrast, activation appeared only after several hours when cells were growing on LB medium. When Pm was induced through the physiological overexpression of XylS, mediated by XylR when this regulator was activated by upper-pathway effectors, the kinetics of transcription from Pm was similar to that of Pu and Ps: maximum values were reached after delays of several hours in rich medium and after several minutes in minimal medium. The delay in the induction of transcription of sigma 54-dependent promoters reflects catabolite inhibition exerted by LB components, since the addition of yeast extracts, Casamino Acids, or several combinations of amino acids dramatically inhibited the synthesis of XylR-controlled sigma 54-dependent promoters. Expression from xylR gene tandem promoters occurred independently of the growth medium used.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Marqués S,Holtel A,Timmis KN,Ramos JLdoi
10.1128/jb.176.9.2517-2524.1994subject
Has Abstractpub_date
1994-05-01 00:00:00pages
2517-24issue
9eissn
0021-9193issn
1098-5530journal_volume
176pub_type
杂志文章abstract::We used Southern blotting to screen a variety of bacterial genes for homology to the kdp genes of Escherichia coli, genes that encode an ATP-driven K+ transport system. We found that most enterobacteria have sequences homologous to those of the three kdp structural genes and the kdpD regulatory gene. A number of dista...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.2.1192-1195.1989
更新日期:1989-02-01 00:00:00
abstract::Containment strategies for outbreaks of invasive Neisseria meningitidis disease are informed by serogroup assays that characterize the polysaccharide capsule. We sought to uncover the genomic basis of conflicting serogroup assay results for an isolate (M16917) from a patient with acute meningococcal disease. To this e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06604-11
更新日期:2012-10-01 00:00:00
abstract::Previous microarray studies have suggested that an indirect mechanism of Fur regulation may be present in meningococcus at the posttranscriptional level through a small regulatory RNA (sRNA) system analogous to that of Escherichia coli and Pseudomonas aeruginosa. Recently, a Fur-regulated sRNA, NrrF, was identified th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00849-08
更新日期:2009-02-01 00:00:00
abstract::Ralston, D. J. (University of California, Berkeley) and M. D. Perry. Inhibitory action of phage K on staphylococcal dehydrogenases. II. Its possible relationship to sensitization and cell lysis. J. Bacteriol. 86:740-748. 1963.-By measuring the reduction of the dye triphenyl tetrazolium chloride to the insoluble red fo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.4.740-748.1963
更新日期:1963-10-01 00:00:00
abstract::Enterococcus faecalis is equipped with two pathways of glycerol dissimilation. Glycerol can either first be phosphorylated by glycerol kinase and then oxidized by glycerol-3-phosphate oxidase (the glpK pathway) or first be oxidized by glycerol dehydrogenase and then phosphorylated by dihydroxyacetone kinase (the dhaK ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00959-09
更新日期:2010-02-01 00:00:00
abstract::The phycocyanin subunit gene cluster is duplicated on the chromosome of the cyanobacterium Anacystis nidulans UTEX 625. The two gene clusters cpcB1A1 (left) and cpcB2A2 (right) are separated by about 2,500 base pairs, and in each cluster the beta-subunit gene is located upstream from the alpha-subunit gene. Filter hyb...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.7.2961-2970.1988
更新日期:1988-07-01 00:00:00
abstract::The Escherichia coli dnaN159 allele encodes a mutant form of the beta-sliding clamp (beta159) that is impaired for interaction with the replicative DNA polymerase (Pol), Pol III. In addition, strains bearing the dnaN159 allele require functional Pol I for viability. We have utilized a combination of genetic and bioche...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00476-07
更新日期:2007-07-01 00:00:00
abstract::Forty years ago, Coulter and Talalay (A. W. Coulter and P. Talalay, J. Biol. Chem. 243:3238-3247, 1968) established the oxygenase-dependent pathway for the degradation of testosterone by aerobes. The oxic testosterone catabolic pathway involves several oxygen-dependent reactions and is not available for anaerobes. Sin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00331-11
更新日期:2011-09-01 00:00:00
abstract::A strain of Neurospora crassa defective in amino acid transport can utilize a variety of amino acids for growth when readily metabolizable nitrogen is limiting. Growth is accompanied by the production of an extracellular deaminase that converts the amino acid to its respective keto acid plus equimolar quantities of ut...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.2.583-589.1984
更新日期:1984-08-01 00:00:00
abstract::This is the first complete genome sequence of the Enterobacter aerogenes species. Here we present the genome sequence of E. aerogenes KCTC 2190, which contains 5,280,350 bp with a G + C content of 54.8 mol%, 4,912 protein-coding genes, and 109 structural RNAs. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00028-12
更新日期:2012-05-01 00:00:00
abstract::Spiralin could not be solubilized in the absence of detergents, and it was shown by charge-shift crossed immunoelectrophoresis that this protein was capable of binding detergents under nondenaturing conditions. These properties indicate the amphiphilic nature of spiralin, which therefore should be regarded as an intri...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.2.738-741.1979
更新日期:1979-11-01 00:00:00
abstract::Halobacterium halobium swims with a polarly inserted motor-driven flagellar bundle. The swimming direction of the cell can be reserved by switching the rotational sense of the bundle. The switch is under the control of photoreceptor and chemoreceptor proteins that act through a branched signal chain. The swimming beha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.6.1971-1977.1991
更新日期:1991-03-01 00:00:00
abstract::Virgibacillus halodenitrificans 1806 is an endospore-forming halophilic bacterium isolated from salterns in Korea. Here, we report the draft genome sequence of V. halodenitrificans 1806, which may reveal the molecular basis of osmoadaptation and insights into carbon and anaerobic metabolism in moderate halophiles. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01280-12
更新日期:2012-11-01 00:00:00
abstract::Respiratory nitrate reductase purified from the cell membrane of Escherichia coli is composed of three subunits, alpha, beta, and gamma, which are encoded, respectively, by the narG, narH, and narI genes of the narGHJI operon. The product of the narJ gene was deduced previously to be a highly charged, acidic protein w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.3.867-872.1992
更新日期:1992-02-01 00:00:00
abstract::A lipoteichoic acid (LTA) was extracted from Listeria monocytogenes (serotype 1) by phenol-water partition and isolated by gel-filtration chromatography. The LTA exhibited amphiphilic properties by changes in gel-filtration mobility in the presence of detergent buffers and after mild base hydrolysis. In a hemagglutina...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.2.809-817.1983
更新日期:1983-11-01 00:00:00
abstract::Deoxyribonucleic acid-deoxyribonucleic acid hybridization studies reveal that the plasmids coding for the production of heat stable and heat labile enteroxtoxins of Escherichia coli, regardless of their origin, have a majority of their polynucleotide sequences in common, but are not related in any significant way to t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.1.234-238.1975
更新日期:1975-01-01 00:00:00
abstract::Chlamydia trachomatis lacks the canonical genes required for the biosynthesis of p-aminobenzoate (pABA), a component of essential folate cofactors. Previous studies revealed a single gene from C. trachomatis, the CT610 gene, that rescues Escherichia coli ΔpabA, ΔpabB, and ΔpabC mutants, which are otherwise auxotrophic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00319-20
更新日期:2020-09-23 00:00:00
abstract::Escherichia coli K-12 can utilize a gamma-glutamyl peptide as an amino acid source, for which gamma-glutamyltranspeptidase (EC 2.3.2.2) is essential. We suggest that the gamma-glutamyl linkage of a gamma-glutamyl peptide is hydrolyzed by gamma-glutamyltranspeptidase located in the periplasmic space, and the released a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.18.6038-6040.1993
更新日期:1993-09-01 00:00:00
abstract::The nucleotide sequence of a 2.0-kilobase DNA segment containing the Clostridium acetobutylicum glnA gene was determined. The upstream region of the glnA gene contained two putative extended promoter consensus sequences (p1 and p2), characteristic of gram-positive bacteria. A third putative extended gram-positive prom...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.1.400-408.1988
更新日期:1988-01-01 00:00:00
abstract::A characteristic of N2-fixing cyanobacteria in symbiotic associations appears to be release of N2-derived NH4+. The specific activity of the primary ammonium-assimilating enzyme, glutamine synthetase (GS), was found to be three- to fourfold lower in Nostoc sp. strain 7801 grown in symbiotic association with the bryoph...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.6.2471-2475.1987
更新日期:1987-06-01 00:00:00
abstract::sigma E is a sporulation-specific sigma factor of Bacillus subtilis that is formed from an inactive precursor protein (pro-sigma E) by the removal of 27 to 29 amino acids from the pro-sigma E amino terminus. By using oligonucleotide-directed mutagenesis, sequential deletions were constructed in the precursor-specific ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.14.4629-4637.1992
更新日期:1992-07-01 00:00:00
abstract::Klebsiella pneumoniae can use nitrate and nitrite as sole nitrogen sources through the nitrate assimilatory pathway. The structural genes for assimilatory nitrate and nitrite reductases together with genes necessary for nitrate transport form an operon, nasFEDCBA. Expression of the nasF operon is regulated both by gen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.16.5077-5085.1994
更新日期:1994-08-01 00:00:00
abstract::The yeast-phase cell cycle of Wangiella dermatitidis was studied using flow microfluorimetry and the deoxyribonucleic acid (DNA) synthesis inhibitor hydroxyurea (HU). Exposure of exponential-phase yeastlike cells to 0.1 M HU for 3 to 6 h resulted in the arrest of the cells in DNA synthesis and produced a nearly homoge...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.2.721-731.1980
更新日期:1980-11-01 00:00:00
abstract::Different electron carriers of the non-desulfoviridin-containing, sulfate-reducing bacterium Desulfovibrio desulfuricans (Norway strain) have been studied. Two nonheme iron proteins, ferredoxin and rubredoxin, have been purified. This ferredoxin contains four atoms of non-heme iron and acid-labile sulfur and six resid...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.1.30-38.1977
更新日期:1977-01-01 00:00:00
abstract::Many of the gene products that participate in nitrogen metabolism are sensitive to nitrogen catabolite repression (NCR), i.e., their expression is decreased to low levels when readily used nitrogen sources such as asparagine are provided. Previous work has shown this NCR sensitivity requires the cis-acting UASNTR elem...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.24.7476-7483.1994
更新日期:1994-12-01 00:00:00
abstract::Two-component signal transduction systems are utilized by prokaryotic and eukaryotic cells to sense and respond to environmental stimuli, both to maintain homeostasis and to rapidly adapt to changing conditions. Studies have begun to emerge that utilize a large-scale mutagenesis approach to analyzing these systems in ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00242-07
更新日期:2007-08-01 00:00:00
abstract::The potassium permeability of Rickettsia prowazekii was characterized by chemical measurement of the intracellular sodium and potassium pools and isotopic flux measurements with 86Rb+ as a tracer. R. prowazekii, in contrast to Escherichia coli, did not maintain a high potassium-to-sodium ratio in their cytoplasm excep...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.1.197-201.1984
更新日期:1984-01-01 00:00:00
abstract::Regulation of the cadA cadmium and zinc resistance determinant of Staphylococcus aureus plasmid pI258 was demonstrated by using gene fusions and direct measurements of transcription. In growth experiments, cells harboring the intact cadA operon were induced with different cations and challenged by an inhibitory concen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.23.7643-7649.1991
更新日期:1991-12-01 00:00:00
abstract::Nakata, H. M. (Washington State University, Pullman). Effect of pH on intermediates produced during growth and sporulation of Bacillus cereus. J. Bacteriol. 86:577-581. 1963.-Cultures of Bacillus cereus strain T, grown in an unbuffered glucose-yeast extract-mineral salts medium and in the same medium buffered at pH 6....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.3.577-581.1963
更新日期:1963-09-01 00:00:00
abstract::The force required to rupture bonds between individual Staphylococcus aureus MSCRAMMs and surfaces coated with extracellular matrix molecules has been quantified by using optical tweezers. The observed binding forces between fibrinogen or fibronectin and S. aureus MSCRAMMs occurred as an approximate integer multiple o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.6.2031-2035.2003
更新日期:2003-03-01 00:00:00