Abstract:
:Many of the gene products that participate in nitrogen metabolism are sensitive to nitrogen catabolite repression (NCR), i.e., their expression is decreased to low levels when readily used nitrogen sources such as asparagine are provided. Previous work has shown this NCR sensitivity requires the cis-acting UASNTR element and trans-acting GLN3. Here, we extend the analysis to include the response of their expression to deletion of the URE2 locus. The expression of these nitrogen catabolic genes becomes, to various degrees, NCR insensitive in the ure2 deletion. This response is shown to be mediated through the GATAA-containing UASNTR element and supports the current idea that the NCR regulatory circuit involves the following steps: environmental signal-->URE2-->GLN3-->UASNTR operation-->NCR-sensitive gene expression. The various responses of the nitrogen catabolic genes' expression to deletion of the URE2 locus also indicate that not all NCR is mediated through URE2.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Coffman JA,el Berry HM,Cooper TGdoi
10.1128/jb.176.24.7476-7483.1994subject
Has Abstractpub_date
1994-12-01 00:00:00pages
7476-83issue
24eissn
0021-9193issn
1098-5530journal_volume
176pub_type
杂志文章abstract::The function of SufI, a well-studied substrate of the TatABC translocase in Escherichia coli, is not known. It was earlier implicated in cell division, based on the finding that multiple copies of sufI suppressed the phenotypes of cells with mutations in ftsI (ftsI23), which encodes a divisomal transpeptidase. Recentl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00773-07
更新日期:2007-11-01 00:00:00
abstract::The secondary structures of the eubacterial RNase P RNAs are being elucidated by a phylogenetic comparative approach. Sequences of genes encoding RNase P RNA from each of the recognized subgroups (alpha, beta, gamma, and delta) of the proteobacteria have now been determined. These sequences allow the refinement, to ne...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.12.3855-3863.1991
更新日期:1991-06-01 00:00:00
abstract::Washed cell suspensions of Aquaspirillum magnetotacticum MS-1, A. itersonii E12639, Bacillus subtilis 6633, and Escherichia coli CSH27 translocated protons in response to the added oxidant O2 or NO3-, with triphenylmethylphosphonium bromide as the permeant ion. Iron respiration-driven proton translocation was observed...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.2.729-731.1986
更新日期:1986-08-01 00:00:00
abstract::Chlamydial lipopolysaccharide (LPS) agglutinated mouse and rabbit erythrocytes but not human, guinea pig, or pronghorn antelope erythrocytes. Hemagglutination was not specific for Chlamydia spp., as rough LPSs from Coxiella burnetii and Escherichia coli also agglutinated erythrocytes from the same animal species. Nona...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.8.3826-3828.1987
更新日期:1987-08-01 00:00:00
abstract::Specific localization of the lysis (L) protein of bacteriophage MS2 in the cell wall of Escherichia coli was determined by immunoelectron microscopy. After induction of the cloned lysis gene, the cells were plasmolyzed, fixed, and embedded in either Epon or Lowicryl K4M. Polyclonal L-protein-specific antiserum was pur...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.6.3331-3336.1989
更新日期:1989-06-01 00:00:00
abstract::Resistance to the antifolates methasquin and amethopterin has been studied in new strains of Streptococcus faecium var. durans. Two methasquin-resistant strains (SF/MQ, SF/MQ(T)) and an amethopterin-resistant strain (SF/AM) were selected independently from the wild-type S. faecium var. durans (SF/O). SF/MQ(T) is a thy...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.110.1.139-145.1972
更新日期:1972-04-01 00:00:00
abstract::An essential component of type III secretion systems (TTSS) is a supramolecular structure termed the needle complex. In Salmonella enterica, at least four proteins make up this structure: InvG, PrgH, PrgK, and PrgI. Another protein, PrgJ, is thought to play a role in the assembly of this structure, but its function is...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.11.3480-3483.2003
更新日期:2003-06-01 00:00:00
abstract::The emerging coverage of diverse habitats by metagenomic shotgun data opens new avenues of discovering functional novelty using computational tools. Here, we apply three different concepts for predicting novel functions within light-mediated microbial pathways in five diverse environments. Using phylogenetic approache...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/JB.01084-08
更新日期:2009-01-01 00:00:00
abstract::Growth and sporulation of a Bacillus subtilis mutant deficient in branched fatty acid synthesis (gene symbol bfmB) were examined. The mutant, which produces an acyl-coenzyme A:acyl carrier protein transacylase with reduced affinity for branched fatty acid primers, could grow in media containing any one of a wide range...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.148.2.480-486.1981
更新日期:1981-11-01 00:00:00
abstract::Integrative and conjugative elements (ICEs), also called conjugative transposons, are genomic islands that excise, self-transfer by conjugation, and integrate in the genome of the recipient bacterium. The current investigation shows the intraspecies conjugative transfer of the first described ICEs in Streptococcus the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01412-08
更新日期:2009-04-01 00:00:00
abstract::An 8,883-bp mini-pXO1 plasmid containing a replicon from Bacillus anthracis pXO1 (181.6 kb) was identified by making large deletions in the original plasmid using a newly developed Cre-loxP system. Portions of the truncated mini-pXO1 were cloned into an Escherichia coli vector unable to replicate in B. anthracis. A 5....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00422-09
更新日期:2009-08-01 00:00:00
abstract::The properties of the anthranilate synthetase complex and its separated subunits were compared in catalyzing the anthranilate synthetase reaction, chorismate + l-glutamine or NH(4) (+) --> anthranilate, and the transferase reaction, anthranilate + 5'-phosphorylribosyl-1-pyrophosphate --> phosphoribosyl anthranilate. I...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.2.734-742.1969
更新日期:1969-02-01 00:00:00
abstract::The alkaline phosphatases of 29 strains of bacteria assigned by various authors to the genera Aerobacter, Klebsiella and Enterobacter were compared by the micro-complement fixation technique. On the basis of phosphatase resemblance, we recommend that all strains hitherto assigned to Aerobacter aerogenes and Enterobact...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.110.3.803-808.1972
更新日期:1972-06-01 00:00:00
abstract::Endonuclease V is specific for single-stranded DNA or for duplex DNA that contains uracil or that is damaged by a variety of agents (B. Demple and S. Linn, J. Biol. Chem. 257:2848-2855, 1982). Thus, it may be a versatile DNA repair enzyme. The protein was purified to apparent homogeneity, and from its N-terminal seque...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.2.310-316.1997
更新日期:1997-01-01 00:00:00
abstract::Caldicellulosiruptor bescii efficiently degrades cellulose, xylan, and native grasses at high temperatures above 70°C under anaerobic conditions. C. bescii extracellularly secretes multidomain glycoside hydrolases along with proteins of unknown function. In this study, we analyzed the C. bescii proteins that bind to t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01897-14
更新日期:2014-11-01 00:00:00
abstract::We investigated the effect of time, temperature, and the presence of sodium chloride, nitrates, and nitrites in the medium on the growth and production of enterotoxin B by Staphylococcus aureus. Assays by the double gel-diffusion method showed that maximal enterotoxin B production occurs at the beginning of the statio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.4.1207-1211.1968
更新日期:1968-04-01 00:00:00
abstract::Pasteurella multocida is the causative agent of a range of diseases with economic importance in production animals. Many systems have been employed to identify virulence factors of P. multocida, including in vivo expression technology (IVET), signature-tagged mutagenesis, and whole-genome expression profiling. In a pr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.7.2278-2285.2005
更新日期:2005-04-01 00:00:00
abstract::We previously demonstrated that mutation of the staphylococcal accessory regulator (sarA) in a clinical isolate of Staphylococcus aureus (UAMS-1) results in an impaired capacity to form a biofilm in vitro (K. E. Beenken, J. S. Blevins, and M. S. Smeltzer, Infect. Immun. 71:4206-4211, 2003). In this report, we used a m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.14.4665-4684.2004
更新日期:2004-07-01 00:00:00
abstract::After coliphage P1-mediated transfer of Tn5-containing plasmid DNA from Escherichia coli to Myxococcus xanthus, transductants were identified which contained plasmid sequences integrated at many sites on the bacterial chromosome. The unaltered plasmid DNA sequences in these transductants were apparently flanked by int...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.10.4939-4941.1988
更新日期:1988-10-01 00:00:00
abstract::Escherichia coli cells harboring a non-attenuated trp-lac operon fusion were used to evaluate the effects of indolmycin on the initiation of transcription at the trp promoter. Indolmycin caused repression in trpR+ strains and in trpR deletion mutants, although higher effector concentrations were required in the latter...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.2.1120-1123.1983
更新日期:1983-02-01 00:00:00
abstract::Commonly used procedures effected the extraction of only 10% of the lipoteichoic acid of stationary-phase cells of Staphylococcus aureus and Streptococcus faecium, unless the cells were first disrupted. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.1.399-402.1982
更新日期:1982-01-01 00:00:00
abstract::OxyR is a DNA binding protein that differentially regulates a cell's response to hydrogen peroxide-mediated oxidative stress. We previously reported that the reduced form of OxyR is sufficient for repression of transcription of agn43 from unmethylated template DNA, which is essential for deoxyadenosine methylase (Dam)...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.7.2203-2209.2003
更新日期:2003-04-01 00:00:00
abstract::The response of Salmonella typhimurium to various forms of environmental stress was examined by using O'Farrell two-dimensional gel electrophoresis. Polypeptides (a total of 110) which quantitatively increased during various starvations, anaerobiosis, or heat shock were identified and cataloged in reference to a stand...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.1.420-424.1986
更新日期:1986-10-01 00:00:00
abstract::The coccoid cyanobacterium Chroococcidiopsis dominates microbial communities in the most extreme arid hot and cold deserts. These communities withstand constraints that result from multiple cycles of drying and wetting and/or prolonged desiccation, through mechanisms which remain poorly understood. Here we describe th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.7.2298-2305.2001
更新日期:2001-04-01 00:00:00
abstract::Methanohalophilus strain Z7302 was previously isolated from a hypersaline environment and grows over a range of NaCl concentrations from 1.7 to 4.4 M. We examined the relationships between cell growth rate, cell volume, and intracellular solute concentrations with increasing salinity. This extremely halophilic methano...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.22.7474-7477.1992
更新日期:1992-11-01 00:00:00
abstract::CTXphi is a filamentous, lysogenic bacteriophage whose genome encodes cholera toxin, the primary virulence factor produced by Vibrio cholerae. CTX prophages in O1 El Tor and O139 strains of V. cholerae are found within arrays of genetically related elements integrated at a single locus within the V. cholerae large chr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.24.6992-6998.2000
更新日期:2000-12-01 00:00:00
abstract::Growth of Streptococcus faecalis in the presence of l-malate resulted in the induction of a "malic enzyme" [l-malate:nicotinamide adenine dinucleotide (NAD) oxidoreductase (decarboxylating), E.C. 1.1.1.39]. Synthesis of the malic enzyme did not appear to be subject to catabolite repression by intermediate products of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.2.705-711.1969
更新日期:1969-05-01 00:00:00
abstract::With the exception of Escherichia coli lysyl-tRNA synthetase, the genes coding for the different aminoacyl-tRNA synthetases in procaryotes are always unique. Here we report on the occurrence and cloning of two genes (thrSv and thrS2), both encoding functional threonyl-tRNA synthetase in Bacillus subtilis. The two prot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.8.4593-4602.1990
更新日期:1990-08-01 00:00:00
abstract::The peptidoglycan layer surrounding the photosynthetic organelles (cyanelles) of the protist Cyanophora paradoxa is thought to be a relic of their cyanobacterial ancestors. The separation of muropeptides by gel filtration and reverse-phase high-performance liquid chromatography revealed four different muropeptide mono...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.2.332-339.1996
更新日期:1996-01-01 00:00:00
abstract::Transcription of the archaeal trkA gene homolog in Methanosarcina mazei S-6 was studied at the optimal growth temperature of 37 degrees C and after heat shock at 45 degrees C. Northern (RNA) blotting results (transcript size) and data from primer extension experiments to map the transcription initiation site indicate ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.21.6077-6082.1995
更新日期:1995-11-01 00:00:00