Abstract:
:We previously demonstrated that mutation of the staphylococcal accessory regulator (sarA) in a clinical isolate of Staphylococcus aureus (UAMS-1) results in an impaired capacity to form a biofilm in vitro (K. E. Beenken, J. S. Blevins, and M. S. Smeltzer, Infect. Immun. 71:4206-4211, 2003). In this report, we used a murine model of catheter-based biofilm formation to demonstrate that a UAMS-1 sarA mutant also has a reduced capacity to form a biofilm in vivo. Surprisingly, mutation of the UAMS-1 ica locus had little impact on biofilm formation in vitro or in vivo. In an effort to identify additional loci that might be relevant to biofilm formation and/or the adaptive response required for persistence of S. aureus within a biofilm, we isolated total cellular RNA from UAMS-1 harvested from a biofilm grown in a flow cell and compared the transcriptional profile of this RNA to RNA isolated from both exponential- and stationary-phase planktonic cultures. Comparisons were done using a custom-made Affymetrix GeneChip representing the genomic complement of six strains of S. aureus (COL, N315, Mu50, NCTC 8325, EMRSA-16 [strain 252], and MSSA-476). The results confirm that the sessile lifestyle associated with persistence within a biofilm is distinct by comparison to the lifestyles of both the exponential and postexponential phases of planktonic culture. Indeed, we identified 48 genes in which expression was induced at least twofold in biofilms over expression under both planktonic conditions. Similarly, we identified 84 genes in which expression was repressed by a factor of at least 2 compared to expression under both planktonic conditions. A primary theme that emerged from the analysis of these genes is that persistence within a biofilm requires an adaptive response that limits the deleterious effects of the reduced pH associated with anaerobic growth conditions.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Beenken KE,Dunman PM,McAleese F,Macapagal D,Murphy E,Projan SJ,Blevins JS,Smeltzer MSdoi
10.1128/JB.186.14.4665-4684.2004keywords:
subject
Has Abstractpub_date
2004-07-01 00:00:00pages
4665-84issue
14eissn
0021-9193issn
1098-5530pii
186/14/4665journal_volume
186pub_type
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更新日期:1971-01-01 00:00:00
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更新日期:1967-04-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/jb.169.4.1651-1655.1987
更新日期:1987-04-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1967-02-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1975-05-01 00:00:00
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更新日期:1964-03-01 00:00:00
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更新日期:1996-07-01 00:00:00
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abstract::Structural analogs of S-adenosylhomocysteine were tested in vitro for inhibition of the yeast S-adenosylmethionine:delta 24-sterol-C-methyltransferase enzyme. A wide inhibitory range by these compounds was observed, suggesting which structural features of the parent compound are important for binding to the enzyme. No...
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更新日期:1981-01-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/jb.177.17.4935-4939.1995
更新日期:1995-09-01 00:00:00
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pub_type: 评论,杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.3.1163-1164.1984
更新日期:1984-06-01 00:00:00
abstract::In Salmonella enterica, the biosynthetic pathways for the generation of purines and the essential cofactor thiamine pyrophosphate branch after sharing five enzymatic steps. Phosphoribosyl amine (PRA) is the first intermediate in the common portion of the pathway and is generated from phosphoribosylpyrophosphate and gl...
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pub_type: 杂志文章
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更新日期:2006-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.19.5741-5747.1996
更新日期:1996-10-01 00:00:00
abstract:UNLABELLED:Succinic semialdehyde (SSA), an important metabolite of γ-aminobutyric acid (GABA), is a ligand of the repressor AttJ regulating the expression of the attJ-attKLM gene cluster in the plant pathogen Agrobacterium tumefaciens. While the response of A. tumefaciens to GABA and the function of attKLM have been ex...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2016-01-11 00:00:00