Sporulation in Bacillus subtilis is independent of membrane fatty acid composition.

Abstract:

:Growth and sporulation of a Bacillus subtilis mutant deficient in branched fatty acid synthesis (gene symbol bfmB) were examined. The mutant, which produces an acyl-coenzyme A:acyl carrier protein transacylase with reduced affinity for branched fatty acid primers, could grow in media containing any one of a wide range of low-molecular-weight fatty acids having branched, cyclic, saturated, or unsaturated carbon chains. The fatty acid composition of cellular lipids depended on the compound used to support growth. Cultures of the bfmB mutant grown in the presence of 3-methylcrotonate contained an unusually high fraction (73%) of straight-chain fatty acids in the cellular lipids. The mutant sporulated with any one of the precursors of branched fatty acids in the medium; isolated spores contained mainly this branched fatty acid and only 10% or less straight-chain fatty acids regardless of the straight-chain fatty acid content of vegetative cells. Exceptional were spores grown in the presence of cyclobutane-carboxylic acid, which contained 28% straight-chain fatty acids. The branched fatty acid composition of spores could be modified greatly by changing the supply of precursors in the medium.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Boudreaux DP,Freese E

doi

10.1128/JB.148.2.480-486.1981

subject

Has Abstract

pub_date

1981-11-01 00:00:00

pages

480-6

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

148

pub_type

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